BacMap

Definition	Dehalococcoides ethenogenes 195, complete genome.
Accession	NC_002936
Length	1,469,720

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The map label for this gene is dnaJ [H]

Identifier: 57233813

GI number: 57233813

Start: 1280306

End: 1281298

Strand: Direct

Name: dnaJ [H]

Synonym: DET1411

Alternate gene names: 57233813

Gene position: 1280306-1281298 (Clockwise)

Preceding gene: 57233832

Following gene: 57233812

Centisome position: 87.11

GC content: 53.17

Gene sequence:

>993_bases
ATGGCTAATGAAAAAAACTTATACGAAACACTGGGCGTAGCCAAAACCGCCAGCGCAGACGAAATCAAGAAGGCATACCG
CAAGCTTGCCCGCAAGTACCACCCGGATTTGAATCCGGGAGATAAGACGGCTGAAGAAACCTTCAAGAAGATTAACCAGG
CTTATGAAGTACTTAACAACCCTGAAAACCGGGCCAAGTATGACAAGTATGGTGACCAGTGGCAGTATGGCGAACAGTTT
GAAAAAGCCCGGGCCAGCCAGCAGCAAACCCAGGGCAACCCCTTTGGGGGGTATGATTTCAGCTTTGGCGGCAGCGAAGG
CGGCGGGTTTGAAGATATTTTGAGCCAGATGCTGGGTGGCCGTATGGGCGGGCGCCGAAGCCGCCCCCAGCCTGGACAAA
GCATTGAACAACCCGTAGACATTTCGCTGGAAGAAGCCGCCAGCGGCACCAGCCGTCTGCTAAGTTTCCAGATGCAAAAA
CCCTGCGAGACCTGCGGAGGCAGCGGGGCAAAAGATAAAAAGGTCTGCCCCACCTGCCAGGGTACAGGTATAAATCCGGG
GATTGAAAGACTGGAAGTCAAAATACCGGCCGGGGTTAAAAATGACTCCAAAATCCGTTTGGCAGGCAAGGGCGGGTATG
GACAATCGGGTGGACAAAGGGGTGACCTATTCCTCCGGATACATGTCCGCCCCAATGATAGATTTGAACGCAAGGATGAT
GACCTGACGGTAAACGTACCGGTAAACCTGACTACCGCCATGCTGGGCGGGGAGATATTTGTCCCCGCACTCAAAGGCAA
ACTAGCCTTGAAAATACCGGCCGAAACCCAGAACGGGCGGACTTTCCGCTTAAAAGGGCAGGGCATCCCCCACTTAGGCA
AAGACGGCAGCGGGGATTTACTGGCCAAAATCAACATTGTGCTGCCGGAAAAACTTACCGCCGCTGAAAGAGACCTTTTT
GAAAAACTGCGCCAGCTTAGGCCGGACAGTTAA

Upstream 100 bases:

>100_bases
ACTGTATTGATACTGTTTTATTAACTTTAATAATATATTGTTGACATTATTCATACCACCATAATATAATAGAAAATAGG
TATTTCCCAGGGGGAATTGT

Downstream 100 bases:

>100_bases
AGGGAGATATTTATGGAAAATGCAAACCAGCCCCGTTATGTAATCTCAGTAGCTGCCGAAATACTGGGTATGCAGACTTA
TACCCTGCGGTATTACGAAA

Product: DnaJ family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MANEKNLYETLGVAKTASADEIKKAYRKLARKYHPDLNPGDKTAEETFKKINQAYEVLNNPENRAKYDKYGDQWQYGEQF
EKARASQQQTQGNPFGGYDFSFGGSEGGGFEDILSQMLGGRMGGRRSRPQPGQSIEQPVDISLEEAASGTSRLLSFQMQK
PCETCGGSGAKDKKVCPTCQGTGINPGIERLEVKIPAGVKNDSKIRLAGKGGYGQSGGQRGDLFLRIHVRPNDRFERKDD
DLTVNVPVNLTTAMLGGEIFVPALKGKLALKIPAETQNGRTFRLKGQGIPHLGKDGSGDLLAKINIVLPEKLTAAERDLF
EKLRQLRPDS

Sequences:

>Translated_330_residues
MANEKNLYETLGVAKTASADEIKKAYRKLARKYHPDLNPGDKTAEETFKKINQAYEVLNNPENRAKYDKYGDQWQYGEQF
EKARASQQQTQGNPFGGYDFSFGGSEGGGFEDILSQMLGGRMGGRRSRPQPGQSIEQPVDISLEEAASGTSRLLSFQMQK
PCETCGGSGAKDKKVCPTCQGTGINPGIERLEVKIPAGVKNDSKIRLAGKGGYGQSGGQRGDLFLRIHVRPNDRFERKDD
DLTVNVPVNLTTAMLGGEIFVPALKGKLALKIPAETQNGRTFRLKGQGIPHLGKDGSGDLLAKINIVLPEKLTAAERDLF
EKLRQLRPDS
>Mature_329_residues
ANEKNLYETLGVAKTASADEIKKAYRKLARKYHPDLNPGDKTAEETFKKINQAYEVLNNPENRAKYDKYGDQWQYGEQFE
KARASQQQTQGNPFGGYDFSFGGSEGGGFEDILSQMLGGRMGGRRSRPQPGQSIEQPVDISLEEAASGTSRLLSFQMQKP
CETCGGSGAKDKKVCPTCQGTGINPGIERLEVKIPAGVKNDSKIRLAGKGGYGQSGGQRGDLFLRIHVRPNDRFERKDDD
LTVNVPVNLTTAMLGGEIFVPALKGKLALKIPAETQNGRTFRLKGQGIPHLGKDGSGDLLAKINIVLPEKLTAAERDLFE
KLRQLRPDS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion. Unfolded proteins bind initially to dnaJ; upon

COG id: COG0484

COG function: function code O; DnaJ-class molecular chaperone with C-terminal Zn finger domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain [H]

Homologues:

Organism=Homo sapiens, GI205360838, Length=343, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI205360840, Length=343, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI194328760, Length=375, Percent_Identity=29.0666666666667, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI194328758, Length=375, Percent_Identity=29.0666666666667, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI4504511, Length=374, Percent_Identity=28.6096256684492, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI7706495, Length=349, Percent_Identity=28.3667621776504, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI5453690, Length=356, Percent_Identity=28.3707865168539, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI5031741, Length=368, Percent_Identity=27.9891304347826, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI6631085, Length=354, Percent_Identity=25.7062146892655, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI56549115, Length=360, Percent_Identity=25.5555555555556, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI201862587, Length=360, Percent_Identity=25.5555555555556, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI201862518, Length=360, Percent_Identity=25.5555555555556, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI39204547, Length=351, Percent_Identity=25.3561253561254, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI194328762, Length=339, Percent_Identity=25.3687315634218, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI29126218, Length=75, Percent_Identity=56, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI24308127, Length=103, Percent_Identity=42.7184466019417, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI194306642, Length=118, Percent_Identity=42.3728813559322, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI194306640, Length=118, Percent_Identity=42.3728813559322, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI23503241, Length=74, Percent_Identity=52.7027027027027, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI56687498, Length=65, Percent_Identity=56.9230769230769, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21553335, Length=140, Percent_Identity=39.2857142857143, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI45504382, Length=77, Percent_Identity=50.6493506493506, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI22749377, Length=71, Percent_Identity=54.9295774647887, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI72534740, Length=118, Percent_Identity=42.3728813559322, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27151736, Length=98, Percent_Identity=43.8775510204082, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI88501736, Length=102, Percent_Identity=43.1372549019608, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI17388799, Length=66, Percent_Identity=56.0606060606061, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4885495, Length=66, Percent_Identity=56.0606060606061, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI47777312, Length=161, Percent_Identity=36.6459627329193, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI68077168, Length=64, Percent_Identity=54.6875, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI68077166, Length=65, Percent_Identity=56.9230769230769, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786197, Length=361, Percent_Identity=36.2880886426593, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1787235, Length=322, Percent_Identity=35.0931677018634, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17553096, Length=353, Percent_Identity=28.328611898017, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17553098, Length=353, Percent_Identity=28.328611898017, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17534355, Length=345, Percent_Identity=27.8260869565217, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17507263, Length=359, Percent_Identity=28.4122562674095, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17554644, Length=70, Percent_Identity=55.7142857142857, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17568643, Length=100, Percent_Identity=46, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI115532476, Length=88, Percent_Identity=39.7727272727273, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI86563276, Length=69, Percent_Identity=52.1739130434783, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI115532378, Length=100, Percent_Identity=41, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17563890, Length=70, Percent_Identity=52.8571428571429, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324265, Length=376, Percent_Identity=30.0531914893617, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321094, Length=394, Percent_Identity=28.4263959390863, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323870, Length=378, Percent_Identity=25.3968253968254, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323119, Length=369, Percent_Identity=27.10027100271, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324252, Length=417, Percent_Identity=22.5419664268585, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322194, Length=71, Percent_Identity=54.9295774647887, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323813, Length=128, Percent_Identity=42.96875, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324321, Length=71, Percent_Identity=50.7042253521127, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320888, Length=73, Percent_Identity=42.4657534246575, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324103, Length=67, Percent_Identity=52.2388059701493, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45552813, Length=377, Percent_Identity=26.2599469496021, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45549272, Length=377, Percent_Identity=26.2599469496021, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45552811, Length=384, Percent_Identity=25.78125, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI85726398, Length=369, Percent_Identity=28.9972899728997, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24658562, Length=357, Percent_Identity=29.6918767507003, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24658555, Length=357, Percent_Identity=29.6918767507003, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129487, Length=370, Percent_Identity=29.4594594594595, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24584014, Length=370, Percent_Identity=29.4594594594595, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19920464, Length=346, Percent_Identity=27.4566473988439, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24646562, Length=386, Percent_Identity=26.9430051813472, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24646560, Length=386, Percent_Identity=26.9430051813472, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24646558, Length=386, Percent_Identity=26.9430051813472, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24646556, Length=386, Percent_Identity=26.9430051813472, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21357547, Length=386, Percent_Identity=26.9430051813472, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI18858081, Length=358, Percent_Identity=26.2569832402235, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17737735, Length=76, Percent_Identity=51.3157894736842, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24668523, Length=76, Percent_Identity=51.3157894736842, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574723, Length=76, Percent_Identity=51.3157894736842, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24654066, Length=103, Percent_Identity=35.9223300970874, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24654070, Length=103, Percent_Identity=35.9223300970874, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24654068, Length=103, Percent_Identity=35.9223300970874, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24654072, Length=103, Percent_Identity=35.9223300970874, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI18859921, Length=67, Percent_Identity=49.2537313432836, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012724
- InterPro:   IPR002939
- InterPro:   IPR001623
- InterPro:   IPR018253
- InterPro:   IPR008971
- InterPro:   IPR015609
- InterPro:   IPR003095
- InterPro:   IPR001305 [H]

Pfam domain/function: PF00226 DnaJ; PF01556 DnaJ_C; PF00684 DnaJ_CXXCXGXG [H]

EC number: NA

Molecular weight: Translated: 36147; Mature: 36016

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00636 DNAJ_1 ; PS50076 DNAJ_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEKNLYETLGVAKTASADEIKKAYRKLARKYHPDLNPGDKTAEETFKKINQAYEVLNN
CCCCHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
PENRAKYDKYGDQWQYGEQFEKARASQQQTQGNPFGGYDFSFGGSEGGGFEDILSQMLGG
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
RMGGRRSRPQPGQSIEQPVDISLEEAASGTSRLLSFQMQKPCETCGGSGAKDKKVCPTCQ
CCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCC
GTGINPGIERLEVKIPAGVKNDSKIRLAGKGGYGQSGGQRGDLFLRIHVRPNDRFERKDD
CCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
DLTVNVPVNLTTAMLGGEIFVPALKGKLALKIPAETQNGRTFRLKGQGIPHLGKDGSGDL
CEEEEECCEEEHHHHCCEEEEEECCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCE
LAKINIVLPEKLTAAERDLFEKLRQLRPDS
EEEEEEECCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANEKNLYETLGVAKTASADEIKKAYRKLARKYHPDLNPGDKTAEETFKKINQAYEVLNN
CCCHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
PENRAKYDKYGDQWQYGEQFEKARASQQQTQGNPFGGYDFSFGGSEGGGFEDILSQMLGG
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
RMGGRRSRPQPGQSIEQPVDISLEEAASGTSRLLSFQMQKPCETCGGSGAKDKKVCPTCQ
CCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCC
GTGINPGIERLEVKIPAGVKNDSKIRLAGKGGYGQSGGQRGDLFLRIHVRPNDRFERKDD
CCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
DLTVNVPVNLTTAMLGGEIFVPALKGKLALKIPAETQNGRTFRLKGQGIPHLGKDGSGDL
CEEEEECCEEEHHHHCCEEEEEECCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCE
LAKINIVLPEKLTAAERDLFEKLRQLRPDS
EEEEEEECCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA