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The map label for this gene is purF [H]

Identifier: 57233809

GI number: 57233809

Start: 1284819

End: 1286237

Strand: Direct

Name: purF [H]

Synonym: DET1415

Alternate gene names: 57233809

Gene position: 1284819-1286237 (Clockwise)

Preceding gene: 57233810

Following gene: 57233808

Centisome position: 87.42

GC content: 52.29

Gene sequence:

>1419_bases
TTGCATGAATCTTGCGGCATTTTCGGTGTATTTGCCCCCGGTCAGGATGTAGCCCGGCTCACATTTTTCGCCCTGTTTGC
CTTGCAGCACAGGGGACAGGAAAGCTCAGGTATTTCTACATCTGACGGGCGGGAACTTAAGCTTTATTCCCAGATGGGTC
TGGTCTCCCATATCTTTACTGAAGATATACTCCGCAAACTGGGCGGACATATTGCCATAGGGCATAACCGTTATTCCACC
ACCGGTTCAAGCCAGCAGATAAACGCCCAGCCATTTCTGATGGGCCAAGGCGACAACGTTATGGCCATAGCCCATAACGG
CAATATAGTTAATTCAGAAGCCCTGAATGCCGAACTAAGCTCTCAGGGATATGTTTTTAAAAGCTCTACAGATACCGAAA
TAATCGGCCAGCTTATCCTGTCAGCAGTTGAAACTGACTGGGTCAAACGGATACGCTATGCCATGAACCGCCTTAAAGGG
GCTTTTTCCTGCACTATTATGACCAAAGACACCCTCTACGCCATGCGTGACCCGCTGGGTGTCCGCCCCCTGTGCCTGGG
CAAAATTCAAGGGGGTTACGTAGTTGCTTCCGAAAGCTGTGCCCTTGACCATATCGGGGCTGATTTCATACGCGAAATAG
AGCCGGGTGAAATTCTGGCTATAAATGAAAACGGGGTAACCAGTTTCAAACAGCCGATTTCCCGCCGGGCTTTGTGCATA
TTTGAATTTATTTATTTTGCCCGCCCTGACAGCCAGATAGACGGGCAGCTTTTATATTCCGCCCGCCAGGCTATGGGGGC
AGAGCTGGCCAGAGAGTACCCGGTAGAAGCGGATTTGGTGATAGGCGTACCTGACTCCGCTACAGCAGCCGGTATAGGTT
ATGCTATAAATTCAGGCATACCCCCGGCCGAAGGCCTTATTAAAAACCGCTACATGGGGCGGACATTTATTCAGCCTGAC
CAGCACCTTCGCGACCTGGGAGTAAAGCTCAAGTTCAACCCCCTGAGGAGTGTTCTGGAAGACAAGCGGGTAGTACTGGT
AGATGACAGCATAGTCCGCGGGACTACCACCCCCCAGGTTATCCGCCTGCTGCGCAAAGCCGGTGCCAAAGAAGTACATA
TGAGGGTATGCGCCCCGCCCATAACCAACCCCTGTTTCTTCGGGGTAGACATGGCCACCCGTTCGGAGCTTATTGCCGCT
AAAATGAGCGTGCCTGAGATACAGAAATACATAGGGGCAGATTCACTTGGTTACCTCAGCCTGCCCGGCCTTATAAAGGC
AGTGGGGCTGCCCAAAGATAACTTCTGCCTGGCCTGTTTCACCGGCGAATACGCCCTGCCGGTTCAGCTGGAGATGGATA
AATTTGCACTCGGCAACCAGCGCGGCAACTGCTGCCAGATGCCATCACCCGGCAGCTAA

Upstream 100 bases:

>100_bases
TTTGGTTTTTAAGAGGTTCGTATTACCCTTTAAATTGACCCGTTTCAAAGCTTGGCGTATAATAAAAAGCTGTCACTCAG
ATATAAAGAGGCTGTCTACT

Downstream 100 bases:

>100_bases
TCCTCCCCCCGCAGGGAGTTATTCCGGATTTAACACCGCCCGAAGTTTTATACCTTCGGCGGTGTTTTTTCTTTGCAGTT
GATAGCTATTTCTGATAATC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MHESCGIFGVFAPGQDVARLTFFALFALQHRGQESSGISTSDGRELKLYSQMGLVSHIFTEDILRKLGGHIAIGHNRYST
TGSSQQINAQPFLMGQGDNVMAIAHNGNIVNSEALNAELSSQGYVFKSSTDTEIIGQLILSAVETDWVKRIRYAMNRLKG
AFSCTIMTKDTLYAMRDPLGVRPLCLGKIQGGYVVASESCALDHIGADFIREIEPGEILAINENGVTSFKQPISRRALCI
FEFIYFARPDSQIDGQLLYSARQAMGAELAREYPVEADLVIGVPDSATAAGIGYAINSGIPPAEGLIKNRYMGRTFIQPD
QHLRDLGVKLKFNPLRSVLEDKRVVLVDDSIVRGTTTPQVIRLLRKAGAKEVHMRVCAPPITNPCFFGVDMATRSELIAA
KMSVPEIQKYIGADSLGYLSLPGLIKAVGLPKDNFCLACFTGEYALPVQLEMDKFALGNQRGNCCQMPSPGS

Sequences:

>Translated_472_residues
MHESCGIFGVFAPGQDVARLTFFALFALQHRGQESSGISTSDGRELKLYSQMGLVSHIFTEDILRKLGGHIAIGHNRYST
TGSSQQINAQPFLMGQGDNVMAIAHNGNIVNSEALNAELSSQGYVFKSSTDTEIIGQLILSAVETDWVKRIRYAMNRLKG
AFSCTIMTKDTLYAMRDPLGVRPLCLGKIQGGYVVASESCALDHIGADFIREIEPGEILAINENGVTSFKQPISRRALCI
FEFIYFARPDSQIDGQLLYSARQAMGAELAREYPVEADLVIGVPDSATAAGIGYAINSGIPPAEGLIKNRYMGRTFIQPD
QHLRDLGVKLKFNPLRSVLEDKRVVLVDDSIVRGTTTPQVIRLLRKAGAKEVHMRVCAPPITNPCFFGVDMATRSELIAA
KMSVPEIQKYIGADSLGYLSLPGLIKAVGLPKDNFCLACFTGEYALPVQLEMDKFALGNQRGNCCQMPSPGS
>Mature_472_residues
MHESCGIFGVFAPGQDVARLTFFALFALQHRGQESSGISTSDGRELKLYSQMGLVSHIFTEDILRKLGGHIAIGHNRYST
TGSSQQINAQPFLMGQGDNVMAIAHNGNIVNSEALNAELSSQGYVFKSSTDTEIIGQLILSAVETDWVKRIRYAMNRLKG
AFSCTIMTKDTLYAMRDPLGVRPLCLGKIQGGYVVASESCALDHIGADFIREIEPGEILAINENGVTSFKQPISRRALCI
FEFIYFARPDSQIDGQLLYSARQAMGAELAREYPVEADLVIGVPDSATAAGIGYAINSGIPPAEGLIKNRYMGRTFIQPD
QHLRDLGVKLKFNPLRSVLEDKRVVLVDDSIVRGTTTPQVIRLLRKAGAKEVHMRVCAPPITNPCFFGVDMATRSELIAA
KMSVPEIQKYIGADSLGYLSLPGLIKAVGLPKDNFCLACFTGEYALPVQLEMDKFALGNQRGNCCQMPSPGS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=513, Percent_Identity=43.6647173489279, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI4826742, Length=184, Percent_Identity=29.3478260869565, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788651, Length=469, Percent_Identity=40.724946695096, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1790167, Length=233, Percent_Identity=31.7596566523605, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=41.1642411642412, Blast_Score=355, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6323958, Length=479, Percent_Identity=34.6555323590814, Blast_Score=245, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6322745, Length=157, Percent_Identity=33.1210191082803, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323730, Length=154, Percent_Identity=33.1168831168831, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24659598, Length=492, Percent_Identity=46.1382113821138, Blast_Score=427, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24659604, Length=465, Percent_Identity=47.0967741935484, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI28573187, Length=494, Percent_Identity=44.9392712550607, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI21357745, Length=187, Percent_Identity=27.807486631016, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51438; Mature: 51438

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHESCGIFGVFAPGQDVARLTFFALFALQHRGQESSGISTSDGRELKLYSQMGLVSHIFT
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHH
EDILRKLGGHIAIGHNRYSTTGSSQQINAQPFLMGQGDNVMAIAHNGNIVNSEALNAELS
HHHHHHCCCEEEECCCCCCCCCCCCEECCCCEEEECCCCEEEEEECCCEECCHHCCCCCC
SQGYVFKSSTDTEIIGQLILSAVETDWVKRIRYAMNRLKGAFSCTIMTKDTLYAMRDPLG
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCC
VRPLCLGKIQGGYVVASESCALDHIGADFIREIEPGEILAINENGVTSFKQPISRRALCI
CCCEEEEECCCCEEEEECCCCHHHHCHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHH
FEFIYFARPDSQIDGQLLYSARQAMGAELAREYPVEADLVIGVPDSATAAGIGYAINSGI
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHCCCC
PPAEGLIKNRYMGRTFIQPDQHLRDLGVKLKFNPLRSVLEDKRVVLVDDSIVRGTTTPQV
CCHHHHHHHCCCCCCCCCCHHHHHHCCCEEEEHHHHHHHCCCCEEEECCCEECCCCCHHH
IRLLRKAGAKEVHMRVCAPPITNPCFFGVDMATRSELIAAKMSVPEIQKYIGADSLGYLS
HHHHHHCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEE
LPGLIKAVGLPKDNFCLACFTGEYALPVQLEMDKFALGNQRGNCCQMPSPGS
CCHHHHHHCCCCCCEEEEEEECCEEEEEEEECCHHCCCCCCCCEECCCCCCC
>Mature Secondary Structure
MHESCGIFGVFAPGQDVARLTFFALFALQHRGQESSGISTSDGRELKLYSQMGLVSHIFT
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHH
EDILRKLGGHIAIGHNRYSTTGSSQQINAQPFLMGQGDNVMAIAHNGNIVNSEALNAELS
HHHHHHCCCEEEECCCCCCCCCCCCEECCCCEEEECCCCEEEEEECCCEECCHHCCCCCC
SQGYVFKSSTDTEIIGQLILSAVETDWVKRIRYAMNRLKGAFSCTIMTKDTLYAMRDPLG
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCC
VRPLCLGKIQGGYVVASESCALDHIGADFIREIEPGEILAINENGVTSFKQPISRRALCI
CCCEEEEECCCCEEEEECCCCHHHHCHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHH
FEFIYFARPDSQIDGQLLYSARQAMGAELAREYPVEADLVIGVPDSATAAGIGYAINSGI
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHCCCC
PPAEGLIKNRYMGRTFIQPDQHLRDLGVKLKFNPLRSVLEDKRVVLVDDSIVRGTTTPQV
CCHHHHHHHCCCCCCCCCCHHHHHHCCCEEEEHHHHHHHCCCCEEEECCCEECCCCCHHH
IRLLRKAGAKEVHMRVCAPPITNPCFFGVDMATRSELIAAKMSVPEIQKYIGADSLGYLS
HHHHHHCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEE
LPGLIKAVGLPKDNFCLACFTGEYALPVQLEMDKFALGNQRGNCCQMPSPGS
CCHHHHHHCCCCCCEEEEEEECCEEEEEEEECCHHCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]