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The map label for this gene is gltA [H]

Identifier: 57233612

GI number: 57233612

Start: 33439

End: 34836

Strand: Direct

Name: gltA [H]

Synonym: DET0038

Alternate gene names: 57233612

Gene position: 33439-34836 (Clockwise)

Preceding gene: 57233613

Following gene: 57233598

Centisome position: 2.28

GC content: 53.15

Gene sequence:

>1398_bases
ATGGCTAAAGCGGGGCTGAACAGAATGGATATGCCTAAACAGGCAGTTAGCGACCGTATTCAAAATTTTTCCGAAGTGGC
CACCGGTTATACCCCGGCTGATGCCCATACCGAAGCTGCCCGCTGTTTGCAGTGCAAAAAGCGTTACTGCGTAGAGGGTT
GTCCGGTAAATATAGATATTCCGGAATTTATTTTGGCTTTGCGGGACGGGAATATGCCCGAAGCTGCCAGGGTACTTAAA
AAGACCAACAGTCTGCCGGGGGTCTGCGGCAGGGTATGTCCCCAGGAAACCCAGTGTGAACAGGCCTGTATATTAAATAA
AAAAGAAGCCCCTATAGCTATAGGCCGTTTGGAACGTTTTGTAGCTGATTGGGAACGCGGACATTCGGCTGAAGTTAATC
TGCCCAAAAGCCTTAAGCCCAAGAGCGGGAAAAAGGCGGCGGTAGTGGGAGCCGGCCCGGCCGGACTTACGGCAGCGGCC
GAGCTTGCCCGTTTGGGGCATGATGTTACCCTGTTTGAGTCTTTGCATACGGCAGGCGGGGTGCTTATGTACGGCATACC
CGAATTCCGCCTTCCCAAAGATATAGTTCAGGGTGAGATAGAGTATGTAAAATCTCTGGGAGTAACGCTGGAGTTAAATT
CGGTAGCGGGGCGGCTCTTTTCGCTGGAAGACCTTTTCAAGCAGGGATTTCAGGCGGTTTTTTTAGCAACCGGAGCCGGT
TTGCCCTTATTTTTAAATGTACAGGGTGAAAACCTGAGCGGGGTATATTCGGCTAACGAATTTCTGACTAGAGTCAACCT
GATGAAGGCTCATAATTTTCCTTCCTCGGATACCCCGGTTAAAAAAGGCAGAAAAGTGGCGGTGATTGGGGGCGGAAACG
TAGCCATGGATGCCGCCCGCTGTGCCTTGCGTCTGGGGGCGGAAGAGGTGAGTATCGTCTACCGCCGTTCGGAGCTGGAA
CTGCCGGCCCGCAAAGAGGAAGTGGACAATGCCCGCGAAGAGGGAATAAAGTTTCATTTCCTGACCAGCCCGGTCCGCTT
TCTGGCTAACGAACAGGGGCAGGTGCGGGCTATGGAATGCCAGAAGATGGCACTTGGCGAACCTGACGCCAGCGGCCGCC
GCCGTCCCATACCGGTGGGCGGCAGCGAATTTGAAATGGATATAGATTTGGCCGTTATTGCCCTGGGTACACGCCCCAAT
CCACTGGTTATGCAGTCCGCCCCGGATTTGCAGGTAAACTCAAACGGCACTGTTCTGGCTGATATAAACGGACAGACTTC
GCACCGGGCGGTTTGGGCCGGTGGGGATATAGTAACCGGCTCCGATACTGTCATAAGTGCTATGGGTGCGGCCAAACGTT
CGGCAGTGGTTATAGACGAATACCTGAGAAGCCTTTAA

Upstream 100 bases:

>100_bases
GTAGACTGGGACGGGCTGCTGGTACGCCAGAAATCTTACGTGGCCGAAGAGGCTGTGGCCGCCGAGAGATGCCGCTGTAA
GGAAGAGGGGTGCGGAAACC

Downstream 100 bases:

>100_bases
TTCCGGCGGCTCAGCAGTAACATAATAAATATTACCAGTACCCATTTCCAGACATACTCCAAAGGGTCGGCTGTCAGTTT
GATTACCAGACTCAGGTTCT

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MAKAGLNRMDMPKQAVSDRIQNFSEVATGYTPADAHTEAARCLQCKKRYCVEGCPVNIDIPEFILALRDGNMPEAARVLK
KTNSLPGVCGRVCPQETQCEQACILNKKEAPIAIGRLERFVADWERGHSAEVNLPKSLKPKSGKKAAVVGAGPAGLTAAA
ELARLGHDVTLFESLHTAGGVLMYGIPEFRLPKDIVQGEIEYVKSLGVTLELNSVAGRLFSLEDLFKQGFQAVFLATGAG
LPLFLNVQGENLSGVYSANEFLTRVNLMKAHNFPSSDTPVKKGRKVAVIGGGNVAMDAARCALRLGAEEVSIVYRRSELE
LPARKEEVDNAREEGIKFHFLTSPVRFLANEQGQVRAMECQKMALGEPDASGRRRPIPVGGSEFEMDIDLAVIALGTRPN
PLVMQSAPDLQVNSNGTVLADINGQTSHRAVWAGGDIVTGSDTVISAMGAAKRSAVVIDEYLRSL

Sequences:

>Translated_465_residues
MAKAGLNRMDMPKQAVSDRIQNFSEVATGYTPADAHTEAARCLQCKKRYCVEGCPVNIDIPEFILALRDGNMPEAARVLK
KTNSLPGVCGRVCPQETQCEQACILNKKEAPIAIGRLERFVADWERGHSAEVNLPKSLKPKSGKKAAVVGAGPAGLTAAA
ELARLGHDVTLFESLHTAGGVLMYGIPEFRLPKDIVQGEIEYVKSLGVTLELNSVAGRLFSLEDLFKQGFQAVFLATGAG
LPLFLNVQGENLSGVYSANEFLTRVNLMKAHNFPSSDTPVKKGRKVAVIGGGNVAMDAARCALRLGAEEVSIVYRRSELE
LPARKEEVDNAREEGIKFHFLTSPVRFLANEQGQVRAMECQKMALGEPDASGRRRPIPVGGSEFEMDIDLAVIALGTRPN
PLVMQSAPDLQVNSNGTVLADINGQTSHRAVWAGGDIVTGSDTVISAMGAAKRSAVVIDEYLRSL
>Mature_464_residues
AKAGLNRMDMPKQAVSDRIQNFSEVATGYTPADAHTEAARCLQCKKRYCVEGCPVNIDIPEFILALRDGNMPEAARVLKK
TNSLPGVCGRVCPQETQCEQACILNKKEAPIAIGRLERFVADWERGHSAEVNLPKSLKPKSGKKAAVVGAGPAGLTAAAE
LARLGHDVTLFESLHTAGGVLMYGIPEFRLPKDIVQGEIEYVKSLGVTLELNSVAGRLFSLEDLFKQGFQAVFLATGAGL
PLFLNVQGENLSGVYSANEFLTRVNLMKAHNFPSSDTPVKKGRKVAVIGGGNVAMDAARCALRLGAEEVSIVYRRSELEL
PARKEEVDNAREEGIKFHFLTSPVRFLANEQGQVRAMECQKMALGEPDASGRRRPIPVGGSEFEMDIDLAVIALGTRPNP
LVMQSAPDLQVNSNGTVLADINGQTSHRAVWAGGDIVTGSDTVISAMGAAKRSAVVIDEYLRSL

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=445, Percent_Identity=31.4606741573034, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI1789606, Length=466, Percent_Identity=36.9098712446352, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1788811, Length=457, Percent_Identity=37.199124726477, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI87082180, Length=459, Percent_Identity=35.7298474945534, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1788468, Length=456, Percent_Identity=33.7719298245614, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789243, Length=425, Percent_Identity=34.3529411764706, Blast_Score=205, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI71984108, Length=452, Percent_Identity=31.1946902654867, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17570289, Length=480, Percent_Identity=27.9166666666667, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17543860, Length=173, Percent_Identity=31.7919075144509, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=468, Percent_Identity=28.8461538461538, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI28574881, Length=475, Percent_Identity=29.4736842105263, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24665539, Length=475, Percent_Identity=29.4736842105263, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24665547, Length=481, Percent_Identity=29.1060291060291, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24665543, Length=481, Percent_Identity=29.1060291060291, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24640763, Length=458, Percent_Identity=30.7860262008734, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI18858217, Length=458, Percent_Identity=30.7860262008734, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 50025; Mature: 49894

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAGLNRMDMPKQAVSDRIQNFSEVATGYTPADAHTEAARCLQCKKRYCVEGCPVNIDI
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
PEFILALRDGNMPEAARVLKKTNSLPGVCGRVCPQETQCEQACILNKKEAPIAIGRLERF
HHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
VADWERGHSAEVNLPKSLKPKSGKKAAVVGAGPAGLTAAAELARLGHDVTLFESLHTAGG
HHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCCC
VLMYGIPEFRLPKDIVQGEIEYVKSLGVTLELNSVAGRLFSLEDLFKQGFQAVFLATGAG
EEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCC
LPLFLNVQGENLSGVYSANEFLTRVNLMKAHNFPSSDTPVKKGRKVAVIGGGNVAMDAAR
CEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEEECCCHHHHHHH
CALRLGAEEVSIVYRRSELELPARKEEVDNAREEGIKFHFLTSPVRFLANEQGQVRAMEC
HHHHCCCHHEEEEEEECCCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHCCCCCEEHHHH
QKMALGEPDASGRRRPIPVGGSEFEMDIDLAVIALGTRPNPLVMQSAPDLQVNSNGTVLA
HHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEECCCCEEECCCCEEEE
DINGQTSHRAVWAGGDIVTGSDTVISAMGAAKRSAVVIDEYLRSL
ECCCCCCCEEEEECCCEECCCHHHHHHHCCHHCCHHHHHHHHHCC
>Mature Secondary Structure 
AKAGLNRMDMPKQAVSDRIQNFSEVATGYTPADAHTEAARCLQCKKRYCVEGCPVNIDI
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
PEFILALRDGNMPEAARVLKKTNSLPGVCGRVCPQETQCEQACILNKKEAPIAIGRLERF
HHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
VADWERGHSAEVNLPKSLKPKSGKKAAVVGAGPAGLTAAAELARLGHDVTLFESLHTAGG
HHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCCC
VLMYGIPEFRLPKDIVQGEIEYVKSLGVTLELNSVAGRLFSLEDLFKQGFQAVFLATGAG
EEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCC
LPLFLNVQGENLSGVYSANEFLTRVNLMKAHNFPSSDTPVKKGRKVAVIGGGNVAMDAAR
CEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEEECCCHHHHHHH
CALRLGAEEVSIVYRRSELELPARKEEVDNAREEGIKFHFLTSPVRFLANEQGQVRAMEC
HHHHCCCHHEEEEEEECCCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHCCCCCEEHHHH
QKMALGEPDASGRRRPIPVGGSEFEMDIDLAVIALGTRPNPLVMQSAPDLQVNSNGTVLA
HHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEECCCCEEECCCCEEEE
DINGQTSHRAVWAGGDIVTGSDTVISAMGAAKRSAVVIDEYLRSL
ECCCCCCCEEEEECCCEECCCHHHHHHHCCHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]