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The map label for this gene is mdeA [H]

Identifier: 56965696

GI number: 56965696

Start: 4095141

End: 4096349

Strand: Direct

Name: mdeA [H]

Synonym: ABC3938

Alternate gene names: 56965696

Gene position: 4095141-4096349 (Clockwise)

Preceding gene: 56965695

Following gene: 56965697

Centisome position: 95.15

GC content: 42.76

Gene sequence:

>1209_bases
ATGAAACCAATCGCTACTGGAATCGAAACAAGCAAAAATCGTTTAGGATTTCAAACAGCCTGTACACATGGAGGGGATCA
TTGGAATAAATCAGGCCATTTAAGTCCTCCTATTTGCCAGTCCACTGCTTTCGAATTCCCGACTACTTCCGAAGGCGCCG
TTCGGGCAGCAGACATTAAGGCCGATGAATTTTATGGCAGGTGGGGGAGCAGAAATGAACGTGAGTTAGAAGCTTTGATT
GCTGCCCTCGAAGAAGCGGACGATGCGCTTTGTGCTTCTTCTGGATTAGGTGCTATCTCCATGATTACACATGCATTTTT
AAATCCAGGAGACCATTTTATCGCTGTTCATAACTGTTACTCTGAAACGAAAATATTACATGAGTCTCTAGCAGAGCAGT
TATCTGTTGATGTCACGTTTATTGACTCTAGTGACATCCATCACTATAAACAGGCGATTCAACCGAATACGAAGTTAGTT
TTTGCTGAAACCCCCGCCAACCCAACTCTAAGTTTAGTAGATATATCTAAAGTAGCTACGCTCGTTCATGAACAAAGCGA
TGCGGTCTTTGTAGTCGATTCAACATTTGCTACACCTTATAATCAAAAACCGTTGAAGCACGGCGCGGATATCGTTGTCC
ATTCTGCCACAAAATATTTAGGTGGGCATTCTGATGTTGTAGCAGGTGTATGTGCTGGTTCAACGGAGTTGATCGATAAA
GTTCGCCATAAATATTCCTTCCATGGTCCGCACCTAGACCCTTTTGGAGCGTGGCTGCTATGTAGAGGTATTCGAACTCT
TGGGCTAAGGATGGAAAAGCATAACGAAAACGCGTTGAAATTAGCTCAATTTCTAGAGACACACCCGAAAATTAGACAAG
TGTTTTATCCATTTTCTGAAAGCCATCCTCAATACAAACTAGCTACTAAACAAATGACTGGCGGCGGTGGCATGATTTGT
TTTGAACTTAAAGATGGTATGGATGGCGGGCTTGGCTTGATTTCAAACGTGAAACTAAACAAGATGGCTGTCAGTTTAGG
CGGGTATGCGAGCACCATTACCCATCCAGCGTCGATGACGCATAATTTATTGCCGAGAGAAGAACGTGAAAAAGGCGGCA
TTACAGATGGCATGATAAGGTTTTCTGTCGGAATTGAAGAATATGAAGATATAAGAGACGACTTATCCCAAGCGCTTGAT
AAAATATAA

Upstream 100 bases:

>100_bases
TTTTCAGTTTGGATAAACAAAAGCCGATGTACCAACTATGACAATGTTTATTAGGCCTGTGTGATGTACACTATCCCAAA
GTTGAAAAGGAGCAAAGATC

Downstream 100 bases:

>100_bases
GGCATTGACGACACGCATGTGAAAATTGGCAGTGAGAGGAGGAAAATGAATGGAACACAAATTGGAAAAAGCTACTTTTT
CAAAAGATATTAAAAATCGA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKPIATGIETSKNRLGFQTACTHGGDHWNKSGHLSPPICQSTAFEFPTTSEGAVRAADIKADEFYGRWGSRNERELEALI
AALEEADDALCASSGLGAISMITHAFLNPGDHFIAVHNCYSETKILHESLAEQLSVDVTFIDSSDIHHYKQAIQPNTKLV
FAETPANPTLSLVDISKVATLVHEQSDAVFVVDSTFATPYNQKPLKHGADIVVHSATKYLGGHSDVVAGVCAGSTELIDK
VRHKYSFHGPHLDPFGAWLLCRGIRTLGLRMEKHNENALKLAQFLETHPKIRQVFYPFSESHPQYKLATKQMTGGGGMIC
FELKDGMDGGLGLISNVKLNKMAVSLGGYASTITHPASMTHNLLPREEREKGGITDGMIRFSVGIEEYEDIRDDLSQALD
KI

Sequences:

>Translated_402_residues
MKPIATGIETSKNRLGFQTACTHGGDHWNKSGHLSPPICQSTAFEFPTTSEGAVRAADIKADEFYGRWGSRNERELEALI
AALEEADDALCASSGLGAISMITHAFLNPGDHFIAVHNCYSETKILHESLAEQLSVDVTFIDSSDIHHYKQAIQPNTKLV
FAETPANPTLSLVDISKVATLVHEQSDAVFVVDSTFATPYNQKPLKHGADIVVHSATKYLGGHSDVVAGVCAGSTELIDK
VRHKYSFHGPHLDPFGAWLLCRGIRTLGLRMEKHNENALKLAQFLETHPKIRQVFYPFSESHPQYKLATKQMTGGGGMIC
FELKDGMDGGLGLISNVKLNKMAVSLGGYASTITHPASMTHNLLPREEREKGGITDGMIRFSVGIEEYEDIRDDLSQALD
KI
>Mature_402_residues
MKPIATGIETSKNRLGFQTACTHGGDHWNKSGHLSPPICQSTAFEFPTTSEGAVRAADIKADEFYGRWGSRNERELEALI
AALEEADDALCASSGLGAISMITHAFLNPGDHFIAVHNCYSETKILHESLAEQLSVDVTFIDSSDIHHYKQAIQPNTKLV
FAETPANPTLSLVDISKVATLVHEQSDAVFVVDSTFATPYNQKPLKHGADIVVHSATKYLGGHSDVVAGVCAGSTELIDK
VRHKYSFHGPHLDPFGAWLLCRGIRTLGLRMEKHNENALKLAQFLETHPKIRQVFYPFSESHPQYKLATKQMTGGGGMIC
FELKDGMDGGLGLISNVKLNKMAVSLGGYASTITHPASMTHNLLPREEREKGGITDGMIRFSVGIEEYEDIRDDLSQALD
KI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=388, Percent_Identity=39.9484536082474, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI299473759, Length=273, Percent_Identity=43.2234432234432, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI25453487, Length=388, Percent_Identity=34.020618556701, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1790375, Length=366, Percent_Identity=37.9781420765027, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI1789383, Length=344, Percent_Identity=28.7790697674419, Blast_Score=160, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI71980708, Length=373, Percent_Identity=38.8739946380697, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17538055, Length=389, Percent_Identity=38.8174807197943, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17560056, Length=389, Percent_Identity=38.560411311054, Blast_Score=231, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6319307, Length=394, Percent_Identity=38.3248730964467, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=29.126213592233, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=29.656862745098, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321170, Length=321, Percent_Identity=28.3489096573209, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=400, Percent_Identity=29.25, Blast_Score=122, Evalue=9e-29,
Organism=Drosophila melanogaster, GI19922554, Length=390, Percent_Identity=39.7435897435897, Blast_Score=268, Evalue=5e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43957; Mature: 43957

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIATGIETSKNRLGFQTACTHGGDHWNKSGHLSPPICQSTAFEFPTTSEGAVRAADIK
CCCCCCCCCCCCHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
ADEFYGRWGSRNERELEALIAALEEADDALCASSGLGAISMITHAFLNPGDHFIAVHNCY
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCH
SETKILHESLAEQLSVDVTFIDSSDIHHYKQAIQPNTKLVFAETPANPTLSLVDISKVAT
HHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEHHHHHHH
LVHEQSDAVFVVDSTFATPYNQKPLKHGADIVVHSATKYLGGHSDVVAGVCAGSTELIDK
HHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECHHHHHCCCCHHHHHHHCCCHHHHHH
VRHKYSFHGPHLDPFGAWLLCRGIRTLGLRMEKHNENALKLAQFLETHPKIRQVFYPFSE
HHHHHCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHCHHHHHHCCCCCC
SHPQYKLATKQMTGGGGMICFELKDGMDGGLGLISNVKLNKMAVSLGGYASTITHPASMT
CCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHCCCEEEHEEEECCCCHHHHCCCHHHH
HNLLPREEREKGGITDGMIRFSVGIEEYEDIRDDLSQALDKI
HHCCCCHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPIATGIETSKNRLGFQTACTHGGDHWNKSGHLSPPICQSTAFEFPTTSEGAVRAADIK
CCCCCCCCCCCCHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
ADEFYGRWGSRNERELEALIAALEEADDALCASSGLGAISMITHAFLNPGDHFIAVHNCY
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCH
SETKILHESLAEQLSVDVTFIDSSDIHHYKQAIQPNTKLVFAETPANPTLSLVDISKVAT
HHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEHHHHHHH
LVHEQSDAVFVVDSTFATPYNQKPLKHGADIVVHSATKYLGGHSDVVAGVCAGSTELIDK
HHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECHHHHHCCCCHHHHHHHCCCHHHHHH
VRHKYSFHGPHLDPFGAWLLCRGIRTLGLRMEKHNENALKLAQFLETHPKIRQVFYPFSE
HHHHHCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHCHHHHHHCCCCCC
SHPQYKLATKQMTGGGGMICFELKDGMDGGLGLISNVKLNKMAVSLGGYASTITHPASMT
CCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHCCCEEEHEEEECCCCHHHHCCCHHHH
HNLLPREEREKGGITDGMIRFSVGIEEYEDIRDDLSQALDKI
HHCCCCHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]