BacMap

Definition	Bacillus clausii KSM-K16, complete genome.
Accession	NC_006582
Length	4,303,871

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The map label for this gene is paaG [C]

Identifier: 56965669

GI number: 56965669

Start: 4070554

End: 4071336

Strand: Direct

Name: paaG [C]

Synonym: ABC3911

Alternate gene names: 56965669

Gene position: 4070554-4071336 (Clockwise)

Preceding gene: 56965667

Following gene: 56965670

Centisome position: 94.58

GC content: 47.64

Gene sequence:

>783_bases
ATGGATCAACCACTTATAACACTCCAAGTCGAGGACAAAGTCGCCACAATTACGATTAACAGGCCAGAAGTCAAAAATGC
ATTAAATGACGCTTGCCACGAACAGTTGTACAACCTGCTTTCGGAAGCAAATGAACGGGAAGACGTGACCGTGATTGTCC
TAACAGGCAGTGGCGATGCTTTTTGTTCAGGTGCTGATTTAAAGTCAATTGATTTAGCGGAACAAGAGCATTTTAATTAT
GGAAAGGCGCTGCGGGAAACCTATAACCGCCTCATTGCTTTCATGGTGCACATTGGCAAGCCGCTTATCGCCGATATAAA
TGGCACTGCCGTAGGCGCTGGTTTGAGCATTGCCCTTGCTTGTGATTTTCGCGTCGCCCGCAAAGAAGCACGATTTGCGC
TAGGCTTTTTAGAGATAGGGCTTGTGCCTGATGCCGGTGCATCCTACTTTTTGCCGCGTTTGATTGGCTACCCGCAAAGC
TTGGAACTAGCAATTAAAGGAATGTTCGACGCCGATGAAGCAAAAGCAATGGGCCTTATCCATAGCATTGGCGAAGCCAC
GCCTTATATTAAACGCTGTAAACAATTGCCTTCTACCGCTTACCGGCTCATGAAACAAAATTTCCGCGAAAGTTTTGACC
ACCACTTAGCGGAACTACTGGAAATGGAAGTCACGGCCCAGCGGGAAGCAGGAGCCTCTCGTGAACACCGCTTGGCGCTT
GAGCGTTTCTTAAAAGTACCGGCAAACGGAAAAGCCCTAAACATGGAAGACTCGAAAAGGTAA

Upstream 100 bases:

>100_bases
CAATTTTAAAAATCCTCATTTAGATTACGCTTCCATATAAGGAAAGGGTTTTCAAGCCTATCAGTCGAATACTAAAAGGA
TAAAGGAGGATGCAATGTCG

Downstream 100 bases:

>100_bases
CGATCCGCTGGCTTATTGGCCAGCGGATCGTTCCTTATGTACTTCTTTTTTTAGCTTTCATCCTGCAGCCACATTTGCCC
GGTGATGATTTGCTTGATTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDQPLITLQVEDKVATITINRPEVKNALNDACHEQLYNLLSEANEREDVTVIVLTGSGDAFCSGADLKSIDLAEQEHFNY
GKALRETYNRLIAFMVHIGKPLIADINGTAVGAGLSIALACDFRVARKEARFALGFLEIGLVPDAGASYFLPRLIGYPQS
LELAIKGMFDADEAKAMGLIHSIGEATPYIKRCKQLPSTAYRLMKQNFRESFDHHLAELLEMEVTAQREAGASREHRLAL
ERFLKVPANGKALNMEDSKR

Sequences:

>Translated_260_residues
MDQPLITLQVEDKVATITINRPEVKNALNDACHEQLYNLLSEANEREDVTVIVLTGSGDAFCSGADLKSIDLAEQEHFNY
GKALRETYNRLIAFMVHIGKPLIADINGTAVGAGLSIALACDFRVARKEARFALGFLEIGLVPDAGASYFLPRLIGYPQS
LELAIKGMFDADEAKAMGLIHSIGEATPYIKRCKQLPSTAYRLMKQNFRESFDHHLAELLEMEVTAQREAGASREHRLAL
ERFLKVPANGKALNMEDSKR
>Mature_260_residues
MDQPLITLQVEDKVATITINRPEVKNALNDACHEQLYNLLSEANEREDVTVIVLTGSGDAFCSGADLKSIDLAEQEHFNY
GKALRETYNRLIAFMVHIGKPLIADINGTAVGAGLSIALACDFRVARKEARFALGFLEIGLVPDAGASYFLPRLIGYPQS
LELAIKGMFDADEAKAMGLIHSIGEATPYIKRCKQLPSTAYRLMKQNFRESFDHHLAELLEMEVTAQREAGASREHRLAL
ERFLKVPANGKALNMEDSKR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=173, Percent_Identity=32.9479768786127, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI20127408, Length=185, Percent_Identity=29.7297297297297, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=30.2752293577982, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=30.2752293577982, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=30.2752293577982, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI37594471, Length=153, Percent_Identity=34.640522875817, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI37594469, Length=155, Percent_Identity=34.1935483870968, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI68989263, Length=202, Percent_Identity=28.2178217821782, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI4757966, Length=181, Percent_Identity=27.6243093922652, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538581, Length=181, Percent_Identity=27.6243093922652, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI25453481, Length=181, Percent_Identity=27.6243093922652, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538573, Length=181, Percent_Identity=27.6243093922652, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757968, Length=181, Percent_Identity=27.6243093922652, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI49355787, Length=181, Percent_Identity=27.6243093922652, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI70995211, Length=180, Percent_Identity=28.8888888888889, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI221307494, Length=164, Percent_Identity=28.6585365853659, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI213417737, Length=236, Percent_Identity=25.8474576271186, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI157694516, Length=236, Percent_Identity=25.8474576271186, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI221136756, Length=164, Percent_Identity=28.6585365853659, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI221136753, Length=164, Percent_Identity=28.6585365853659, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI31542718, Length=176, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4502327, Length=177, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=32, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787659, Length=209, Percent_Identity=33.9712918660287, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788597, Length=177, Percent_Identity=28.8135593220339, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788682, Length=183, Percent_Identity=31.6939890710383, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI221142681, Length=186, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=28.4210526315789, Blast_Score=74, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=30.4526748971193, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=181, Percent_Identity=32.0441988950276, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=173, Percent_Identity=34.6820809248555, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=202, Percent_Identity=30.6930693069307, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=185, Percent_Identity=30.8108108108108, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=27.9527559055118, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=221, Percent_Identity=28.5067873303167, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=150, Percent_Identity=36, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=150, Percent_Identity=36, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=175, Percent_Identity=29.7142857142857, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=159, Percent_Identity=31.4465408805031, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=159, Percent_Identity=31.4465408805031, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=159, Percent_Identity=31.4465408805031, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=184, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=163, Percent_Identity=26.3803680981595, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20129971, Length=218, Percent_Identity=29.3577981651376, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24653477, Length=218, Percent_Identity=29.3577981651376, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24654903, Length=214, Percent_Identity=29.9065420560748, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24653139, Length=173, Percent_Identity=31.2138728323699, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28571730, Length=142, Percent_Identity=35.2112676056338, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28571729, Length=142, Percent_Identity=35.2112676056338, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21357171, Length=182, Percent_Identity=32.4175824175824, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24650670, Length=185, Percent_Identity=30.8108108108108, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920382, Length=183, Percent_Identity=28.4153005464481, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19921000, Length=179, Percent_Identity=27.9329608938547, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24583077, Length=179, Percent_Identity=27.9329608938547, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24583079, Length=179, Percent_Identity=27.9329608938547, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28778; Mature: 28778

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQPLITLQVEDKVATITINRPEVKNALNDACHEQLYNLLSEANEREDVTVIVLTGSGDA
CCCCEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
FCSGADLKSIDLAEQEHFNYGKALRETYNRLIAFMVHIGKPLIADINGTAVGAGLSIALA
EECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCEEEEEE
CDFRVARKEARFALGFLEIGLVPDAGASYFLPRLIGYPQSLELAIKGMFDADEAKAMGLI
ECHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCHHHHHEECCCCCHHHHHHHHH
HSIGEATPYIKRCKQLPSTAYRLMKQNFRESFDHHLAELLEMEVTAQREAGASREHRLAL
HHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ERFLKVPANGKALNMEDSKR
HHHHHCCCCCCEECCCCCCC
>Mature Secondary Structure
MDQPLITLQVEDKVATITINRPEVKNALNDACHEQLYNLLSEANEREDVTVIVLTGSGDA
CCCCEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
FCSGADLKSIDLAEQEHFNYGKALRETYNRLIAFMVHIGKPLIADINGTAVGAGLSIALA
EECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCEEEEEE
CDFRVARKEARFALGFLEIGLVPDAGASYFLPRLIGYPQSLELAIKGMFDADEAKAMGLI
ECHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCHHHHHEECCCCCHHHHHHHHH
HSIGEATPYIKRCKQLPSTAYRLMKQNFRESFDHHLAELLEMEVTAQREAGASREHRLAL
HHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ERFLKVPANGKALNMEDSKR
HHHHHCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA