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The map label for this gene is pucF [H]

Identifier: 56965543

GI number: 56965543

Start: 3946674

End: 3947918

Strand: Direct

Name: pucF [H]

Synonym: ABC3785

Alternate gene names: 56965543

Gene position: 3946674-3947918 (Clockwise)

Preceding gene: 56965542

Following gene: 56965544

Centisome position: 91.7

GC content: 51.08

Gene sequence:

>1245_bases
ATGAACACCAATAAGCTAACAGTCAATCACGAGCGGCTTAAACAAACGCTCCAAACGTTCGCAGCATTTGGGGCAACAGG
CAACAATGGCGTCACACGCCTTGCCCTGTCTGAAGAAGACCAGCAAGCACGGGCTTATTTGCGCCAGCAATGTGAAGCGC
TCGGGCTTGTAGTAACGACGGACGATCTCGGAAACATGTACAGTGTAATTCCAGGAAAACAGCGCGATGTCCCGCCAGTC
TACCTTGGTTCCCACCTTGATACGGTAAAAAAAGGAGGGCGTTTCGATGGTGTCCTCGGTGTTGCAGGTGCCTTAGAAGT
ACTACGCACAGTAGTCGAACATAAGCTTGAATTAAATGTGCCCCTCGGACTGATCAATTTCACCAACGAGGAAGGCGCCC
GTTTTGAGCCTTCGATGATGGCATCAGGGATTTTGGCTGGAAAATTTAGCAAAGACGCAATGTTGCAATCAACAGACCAA
GATGGCGTTTCTTTTGGAGAGGCTCTGGCGGCGAGTGGTTTTGCTGGCTCGGCCCACCATCGGCTGACGGACGCAAGCGC
TTTTTTAGAACTGCATATCGAACAAGGACCAGTTTTAGAAGCAGAAAAGCAAACCATTGGCGCCGTTGAGTGCGTTGTGG
GCATGGTTTGTTACGAAATCGAGATTTGCGGAAAATCCAACCACGCTGGAACAACGCCGCAACGGATGCGTCATGATGCA
CTAACCGCAGCCAACGCCTGCATGACCGCTTTCCACGAAAAGCTAGCACCGTTAGACGACACGCTCGTTTATACGATCGG
ACGCTTTAATGTGTTTCCGAACATCCATACAGTCATTCCTGGCCGTGCTGTCTTCACTCTTGAAGCTAGGCATCAAGACG
AAGCGGTACTGGCCGCAGTAGAGACTGTGATTCAGGAAGTCGCCGGAGCGTGCTCCCTTCCTTGTCAGGCAACAAAGCGA
TGGAGCCGAGAGACGGTTTGGTTTAACAACAGCCTCGTCGCCCAAGTAGAAGAATCGGCTAAAGCACTCGGCTATCCTTG
TAAACGCATGGTGAGCGGCGCTGGCCACGATGCTCAATTTATTTCTTCCCTTGTCCCGACAGCCATGATTTTTGTTCCAA
GCCGGGGAGGAATTAGTCACGCTGAGGAAGAATGGACTTCTTGGGAGGACTGCGAAAAGGGCGTCAATGTCCTATTAGAA
ACGGCATTGAAACGAACGATGCACGAGGAGGCGATTAAACAGTGA

Upstream 100 bases:

>100_bases
ACCTTTTTAGCAGCAAATGCCAACGCACGTAAATATGCTTCAACCCTTTTTTGTAAAGGGCAAGCCTGGCAAACCGTTAC
TAGCCAAGGAGGCAATGCCC

Downstream 100 bases:

>100_bases
ACACTGGAATGGAACCGACATTGGCCAAAAAAGACGGGCAATATGTCTGGCATGCGATGCAACGTCCAGGAGCCGCGCCT
CTTATCGCCGATTCAGGTGA

Product: allantoate amidohydrolase

Products: NA

Alternate protein names: Allantoate deiminase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MNTNKLTVNHERLKQTLQTFAAFGATGNNGVTRLALSEEDQQARAYLRQQCEALGLVVTTDDLGNMYSVIPGKQRDVPPV
YLGSHLDTVKKGGRFDGVLGVAGALEVLRTVVEHKLELNVPLGLINFTNEEGARFEPSMMASGILAGKFSKDAMLQSTDQ
DGVSFGEALAASGFAGSAHHRLTDASAFLELHIEQGPVLEAEKQTIGAVECVVGMVCYEIEICGKSNHAGTTPQRMRHDA
LTAANACMTAFHEKLAPLDDTLVYTIGRFNVFPNIHTVIPGRAVFTLEARHQDEAVLAAVETVIQEVAGACSLPCQATKR
WSRETVWFNNSLVAQVEESAKALGYPCKRMVSGAGHDAQFISSLVPTAMIFVPSRGGISHAEEEWTSWEDCEKGVNVLLE
TALKRTMHEEAIKQ

Sequences:

>Translated_414_residues
MNTNKLTVNHERLKQTLQTFAAFGATGNNGVTRLALSEEDQQARAYLRQQCEALGLVVTTDDLGNMYSVIPGKQRDVPPV
YLGSHLDTVKKGGRFDGVLGVAGALEVLRTVVEHKLELNVPLGLINFTNEEGARFEPSMMASGILAGKFSKDAMLQSTDQ
DGVSFGEALAASGFAGSAHHRLTDASAFLELHIEQGPVLEAEKQTIGAVECVVGMVCYEIEICGKSNHAGTTPQRMRHDA
LTAANACMTAFHEKLAPLDDTLVYTIGRFNVFPNIHTVIPGRAVFTLEARHQDEAVLAAVETVIQEVAGACSLPCQATKR
WSRETVWFNNSLVAQVEESAKALGYPCKRMVSGAGHDAQFISSLVPTAMIFVPSRGGISHAEEEWTSWEDCEKGVNVLLE
TALKRTMHEEAIKQ
>Mature_414_residues
MNTNKLTVNHERLKQTLQTFAAFGATGNNGVTRLALSEEDQQARAYLRQQCEALGLVVTTDDLGNMYSVIPGKQRDVPPV
YLGSHLDTVKKGGRFDGVLGVAGALEVLRTVVEHKLELNVPLGLINFTNEEGARFEPSMMASGILAGKFSKDAMLQSTDQ
DGVSFGEALAASGFAGSAHHRLTDASAFLELHIEQGPVLEAEKQTIGAVECVVGMVCYEIEICGKSNHAGTTPQRMRHDA
LTAANACMTAFHEKLAPLDDTLVYTIGRFNVFPNIHTVIPGRAVFTLEARHQDEAVLAAVETVIQEVAGACSLPCQATKR
WSRETVWFNNSLVAQVEESAKALGYPCKRMVSGAGHDAQFISSLVPTAMIFVPSRGGISHAEEEWTSWEDCEKGVNVLLE
TALKRTMHEEAIKQ

Specific function: Involved in the anaerobic utilization of allantoin. Converts allantoate to (S)-ureidoglycolate and ammonia [H]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=402, Percent_Identity=35.0746268656716, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF01546 Peptidase_M20 [H]

EC number: 3.5.3.- [C]

Molecular weight: Translated: 44881; Mature: 44881

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNKLTVNHERLKQTLQTFAAFGATGNNGVTRLALSEEDQQARAYLRQQCEALGLVVTT
CCCCCEEECHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCEEEEE
DDLGNMYSVIPGKQRDVPPVYLGSHLDTVKKGGRFDGVLGVAGALEVLRTVVEHKLELNV
CCCCCHHHHCCCCCCCCCCEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCC
PLGLINFTNEEGARFEPSMMASGILAGKFSKDAMLQSTDQDGVSFGEALAASGFAGSAHH
CEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCHH
RLTDASAFLELHIEQGPVLEAEKQTIGAVECVVGMVCYEIEICGKSNHAGTTPQRMRHDA
HHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHH
LTAANACMTAFHEKLAPLDDTLVYTIGRFNVFPNIHTVIPGRAVFTLEARHQDEAVLAAV
HHHHHHHHHHHHHHHCCCCCHHEEEEEEEEECCCCCEECCCCEEEEEECCCCCHHHHHHH
ETVIQEVAGACSLPCQATKRWSRETVWFNNSLVAQVEESAKALGYPCKRMVSGAGHDAQF
HHHHHHHHHHCCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHH
ISSLVPTAMIFVPSRGGISHAEEEWTSWEDCEKGVNVLLETALKRTMHEEAIKQ
HHHHHCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTNKLTVNHERLKQTLQTFAAFGATGNNGVTRLALSEEDQQARAYLRQQCEALGLVVTT
CCCCCEEECHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCEEEEE
DDLGNMYSVIPGKQRDVPPVYLGSHLDTVKKGGRFDGVLGVAGALEVLRTVVEHKLELNV
CCCCCHHHHCCCCCCCCCCEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCC
PLGLINFTNEEGARFEPSMMASGILAGKFSKDAMLQSTDQDGVSFGEALAASGFAGSAHH
CEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCHH
RLTDASAFLELHIEQGPVLEAEKQTIGAVECVVGMVCYEIEICGKSNHAGTTPQRMRHDA
HHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHH
LTAANACMTAFHEKLAPLDDTLVYTIGRFNVFPNIHTVIPGRAVFTLEARHQDEAVLAAV
HHHHHHHHHHHHHHHCCCCCHHEEEEEEEEECCCCCEECCCCEEEEEECCCCCHHHHHHH
ETVIQEVAGACSLPCQATKRWSRETVWFNNSLVAQVEESAKALGYPCKRMVSGAGHDAQF
HHHHHHHHHHCCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHH
ISSLVPTAMIFVPSRGGISHAEEEWTSWEDCEKGVNVLLETALKRTMHEEAIKQ
HHHHHCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]