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The map label for this gene is aegA [H]

Identifier: 56965539

GI number: 56965539

Start: 3942292

End: 3943638

Strand: Direct

Name: aegA [H]

Synonym: ABC3781

Alternate gene names: 56965539

Gene position: 3942292-3943638 (Clockwise)

Preceding gene: 56965536

Following gene: 56965540

Centisome position: 91.6

GC content: 50.63

Gene sequence:

>1347_bases
ATGGAACGATCGGAATCCATCCCATTGAATTTCCAAGAAGTCGAACCGCCATTATCCACCCAAGAAGCGACAGATGAAGC
AACTAGGTGCCTTTATTGCTATGATGCACCATGCATTACTGCATGTCCTACCGGAATTGACATCCCCTCATTCATTCAAA
AAATCACAACAAATAATTTGACAGGCTCCGCTCGGACGATCATGGAGGCTAACCCGATCGGAGCCAGTTGCGCCAGGGTT
TGCCCGACGGAAGAACTATGTGAAGGCGCGTGTGTCCTGAATCAGACATCGAAACCGATTATGATCGGCAAATTGCAGCG
TTATGCGACTGACTGGGCGATCCAAAACGAGAAACCATTGTTTACAGCGGGCAAACGAAACGGACAGAAAGTAGCGGTTG
TCGGCGGAGGGCCAGCAGGGTTATCTGCCGCGCGGGAGCTTGCTTTGCTTGGCTATGCGGTAACCGTTTATGAGGCAGAA
ACGGAAGCCGGCGGCCTTGATACGTACGGCATCGTCTCATTCCGTCTGCCAAAGCGCATCTCTTTATGGGAAGTCGAACA
GGTCAAGTCTCTCGGCGTCTCCATTCAGACAAACACGCGTATTGGAGTCGACCTTTCTCTTGCAGAGCTACAGCAATCCT
TTGATGCCGTTGTTTTAGCGGTTGGCATGGCAAACGTGCCCCCTCTCGGCATTCCCGGCGAAGAACTTCATGGTGTTTAT
GATGCCATTGATCTTGTAAAGGCAACGAAGTCCACCACGTTGCCCCAACAATTCCAAGGAAAGCGAGGCGTTGTCATCGG
CGCCGGCAACACAGCGATTGATGGAGCGACATGCGCCGTCCGTTTAGGGGCGGAAAACGTCAAAGTTCTGTATCGCCGCA
CAGAAGCAGAAATGACTGCTTACCAATTTGAATATGAGTTTGCCAAACAAGATGGCGTTGAGTTTCGCTGGCTTGTCTCC
CCTATCGCTGTACTTGGTGATGAAAAAGGGCGCGTAACGGCTTTGAAATGCGCCGAAATGGCTTTGACTGAGGCAGATGA
AAGCGGACGAAGAACACCGATTCCAACAGGAGCGACATTTGTCATCAAAACAGACTTTATCATTAAAGCGATTGGGCAAG
CGCGCCATCGTTGGCTCGAAGACGTTGGCATTGCTACACACAACGGTGTTATTTCAATTGACCATCACTATGAAACATCT
GTAAAAGGCGTTTTTGCCTGCGGCGACGCGATATTCGGCCAAGGGCAAGGTGAAGCAATGGTAGTTGCGGCTGCCGAACA
AGGAAAGCAAGTGGCGTACGCAATTGATAAAAAACGACGCAGCCAACGGGCTGAAACGAGCGCATAA

Upstream 100 bases:

>100_bases
AAATCTAACGATAACAGTGATTTTCAATTATTTCTATCATATAGTTATTTAAATACCAGCTTGTGCCTAACTGGTACTGT
CACAAAAGGGAGGCTATTAT

Downstream 100 bases:

>100_bases
GGAGGAATTCGATGGCTGACCTCATTAGCAATTTAGCTGGCATTACATCGCCTAACCCATTTTGGCTTGCCAGCGCTCCG
CCGACCAACTCTGGCTATCA

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MERSESIPLNFQEVEPPLSTQEATDEATRCLYCYDAPCITACPTGIDIPSFIQKITTNNLTGSARTIMEANPIGASCARV
CPTEELCEGACVLNQTSKPIMIGKLQRYATDWAIQNEKPLFTAGKRNGQKVAVVGGGPAGLSAARELALLGYAVTVYEAE
TEAGGLDTYGIVSFRLPKRISLWEVEQVKSLGVSIQTNTRIGVDLSLAELQQSFDAVVLAVGMANVPPLGIPGEELHGVY
DAIDLVKATKSTTLPQQFQGKRGVVIGAGNTAIDGATCAVRLGAENVKVLYRRTEAEMTAYQFEYEFAKQDGVEFRWLVS
PIAVLGDEKGRVTALKCAEMALTEADESGRRTPIPTGATFVIKTDFIIKAIGQARHRWLEDVGIATHNGVISIDHHYETS
VKGVFACGDAIFGQGQGEAMVVAAAEQGKQVAYAIDKKRRSQRAETSA

Sequences:

>Translated_448_residues
MERSESIPLNFQEVEPPLSTQEATDEATRCLYCYDAPCITACPTGIDIPSFIQKITTNNLTGSARTIMEANPIGASCARV
CPTEELCEGACVLNQTSKPIMIGKLQRYATDWAIQNEKPLFTAGKRNGQKVAVVGGGPAGLSAARELALLGYAVTVYEAE
TEAGGLDTYGIVSFRLPKRISLWEVEQVKSLGVSIQTNTRIGVDLSLAELQQSFDAVVLAVGMANVPPLGIPGEELHGVY
DAIDLVKATKSTTLPQQFQGKRGVVIGAGNTAIDGATCAVRLGAENVKVLYRRTEAEMTAYQFEYEFAKQDGVEFRWLVS
PIAVLGDEKGRVTALKCAEMALTEADESGRRTPIPTGATFVIKTDFIIKAIGQARHRWLEDVGIATHNGVISIDHHYETS
VKGVFACGDAIFGQGQGEAMVVAAAEQGKQVAYAIDKKRRSQRAETSA
>Mature_448_residues
MERSESIPLNFQEVEPPLSTQEATDEATRCLYCYDAPCITACPTGIDIPSFIQKITTNNLTGSARTIMEANPIGASCARV
CPTEELCEGACVLNQTSKPIMIGKLQRYATDWAIQNEKPLFTAGKRNGQKVAVVGGGPAGLSAARELALLGYAVTVYEAE
TEAGGLDTYGIVSFRLPKRISLWEVEQVKSLGVSIQTNTRIGVDLSLAELQQSFDAVVLAVGMANVPPLGIPGEELHGVY
DAIDLVKATKSTTLPQQFQGKRGVVIGAGNTAIDGATCAVRLGAENVKVLYRRTEAEMTAYQFEYEFAKQDGVEFRWLVS
PIAVLGDEKGRVTALKCAEMALTEADESGRRTPIPTGATFVIKTDFIIKAIGQARHRWLEDVGIATHNGVISIDHHYETS
VKGVFACGDAIFGQGQGEAMVVAAAEQGKQVAYAIDKKRRSQRAETSA

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=468, Percent_Identity=33.7606837606838, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI237757300, Length=112, Percent_Identity=45.5357142857143, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788811, Length=444, Percent_Identity=34.6846846846847, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI87082180, Length=442, Percent_Identity=35.0678733031674, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1789606, Length=445, Percent_Identity=33.0337078651685, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1788468, Length=429, Percent_Identity=34.032634032634, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1789243, Length=410, Percent_Identity=32.9268292682927, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71984108, Length=420, Percent_Identity=34.5238095238095, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17570289, Length=451, Percent_Identity=26.1640798226164, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320030, Length=462, Percent_Identity=27.9220779220779, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24640763, Length=420, Percent_Identity=33.8095238095238, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI18858217, Length=420, Percent_Identity=33.8095238095238, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24665547, Length=457, Percent_Identity=26.4770240700219, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24665543, Length=457, Percent_Identity=26.4770240700219, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI28574881, Length=457, Percent_Identity=26.4770240700219, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24665539, Length=457, Percent_Identity=26.4770240700219, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 48125; Mature: 48125

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERSESIPLNFQEVEPPLSTQEATDEATRCLYCYDAPCITACPTGIDIPSFIQKITTNNL
CCCCCCCCCCHHHCCCCCCCCCCCCHHHEEEEEECCCCCEECCCCCCHHHHHHHHHCCCC
TGSARTIMEANPIGASCARVCPTEELCEGACVLNQTSKPIMIGKLQRYATDWAIQNEKPL
CCCCEEEEECCCCCCHHHHCCCCHHHHCCCEEECCCCCCEEEHHHHHHHHHHEECCCCCE
FTAGKRNGQKVAVVGGGPAGLSAARELALLGYAVTVYEAETEAGGLDTYGIVSFRLPKRI
EECCCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEEEECCCCCCCCCEEEEEEECCCCC
SLWEVEQVKSLGVSIQTNTRIGVDLSLAELQQSFDAVVLAVGMANVPPLGIPGEELHGVY
CHHHHHHHHHCCCEEEECCEEEEEEEHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHH
DAIDLVKATKSTTLPQQFQGKRGVVIGAGNTAIDGATCAVRLGAENVKVLYRRTEAEMTA
HHHHHHHHHCCCCCCHHHCCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEEEHHHHEEE
YQFEYEFAKQDGVEFRWLVSPIAVLGDEKGRVTALKCAEMALTEADESGRRTPIPTGATF
EEEEEEEHHCCCCEEEEEHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCEE
VIKTDFIIKAIGQARHRWLEDVGIATHNGVISIDHHYETSVKGVFACGDAIFGQGQGEAM
EEECHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCCCCCEEEEECCHHCCCCCCCEE
VVAAAEQGKQVAYAIDKKRRSQRAETSA
EEEECCCCCEEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure
MERSESIPLNFQEVEPPLSTQEATDEATRCLYCYDAPCITACPTGIDIPSFIQKITTNNL
CCCCCCCCCCHHHCCCCCCCCCCCCHHHEEEEEECCCCCEECCCCCCHHHHHHHHHCCCC
TGSARTIMEANPIGASCARVCPTEELCEGACVLNQTSKPIMIGKLQRYATDWAIQNEKPL
CCCCEEEEECCCCCCHHHHCCCCHHHHCCCEEECCCCCCEEEHHHHHHHHHHEECCCCCE
FTAGKRNGQKVAVVGGGPAGLSAARELALLGYAVTVYEAETEAGGLDTYGIVSFRLPKRI
EECCCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEEEECCCCCCCCCEEEEEEECCCCC
SLWEVEQVKSLGVSIQTNTRIGVDLSLAELQQSFDAVVLAVGMANVPPLGIPGEELHGVY
CHHHHHHHHHCCCEEEECCEEEEEEEHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHH
DAIDLVKATKSTTLPQQFQGKRGVVIGAGNTAIDGATCAVRLGAENVKVLYRRTEAEMTA
HHHHHHHHHCCCCCCHHHCCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEEEHHHHEEE
YQFEYEFAKQDGVEFRWLVSPIAVLGDEKGRVTALKCAEMALTEADESGRRTPIPTGATF
EEEEEEEHHCCCCEEEEEHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCEE
VIKTDFIIKAIGQARHRWLEDVGIATHNGVISIDHHYETSVKGVFACGDAIFGQGQGEAM
EEECHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCCCCCEEEEECCHHCCCCCCCEE
VVAAAEQGKQVAYAIDKKRRSQRAETSA
EEEECCCCCEEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]