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The map label for this gene is lmrB [H]

Identifier: 56965193

GI number: 56965193

Start: 3558234

End: 3559715

Strand: Direct

Name: lmrB [H]

Synonym: ABC3431

Alternate gene names: 56965193

Gene position: 3558234-3559715 (Clockwise)

Preceding gene: 56965191

Following gene: 56965196

Centisome position: 82.68

GC content: 47.5

Gene sequence:

>1482_bases
ATGGCTCACGTTTCACAAGAACCATCGGCGGCAGCGACGACGGAAACCGTACAAGTCAAAGTGCTGCCTTTACTTGCTGT
ATTATTATCTGGGGCATTTGTCGCTATCTTAAGCGAAACCATTTTAAATGTTGCCTTAAACGCTATTATGAGCGATTTTG
GTGTGGCTTCCAGTACAGCCCAATGGCTTGTTACAGGCTATATGTTAGTAATAGGCACATTAATCCCCATCTCTGCGTAT
TTTATGCAACGTTTTACGACGCGGCAATTGTTTATTACAGCGATGTCGCTGTTTACACTCGGCACATTAATAGCTGGATT
TAGTCCAGTATTTGCTATGTTGCTTGTCGGCCGGTTGACGCAGGCAGTCGGGACAGCGCTCATGATTCCCCTGCTCACGA
ACGTCATTTTAGCTGTTATTCCAATCGAGCGCCGAGGCGCTGCCATGGGGATGGTCGGACTTGTCATTATGTTTGCCCCA
GCAATCGGACCGACTGTGTCTGGTATCATTGTCGATAGCCTGTCATGGCGCTGGCTTTTTTATTTTATTACGCCCATCTC
TTTATTGTCGTTGCTATTTGGCGTAAAAGTTTTACGCAATATCGGAGAGACATCACGGCCGAAACTTGATGTCGTTTCGT
TTATCCTTTCGACACTCGGTTTTGGTGGAATTGTCTTTGGCTTTAGTGCGGCAGGCAAAGGGGACGCCTCTTTTACAGAC
CCCCTCGTCTATTCGACTCTGTTAATTGGTTTTGCGAGCCTCTGCATTTTTGGCTGGCGCCAACTGAAATTGGAAACGCC
GCTGTTAGACATACGTGTTTTCCGCTACCCGATGTTTCTAATTGGCCTTATTGTCGTGATGCTCGTCATGATGACAATGT
TCGCCATGATGCTCATTATGCCGATTTATATGCAAGGGGCATTGTTATTTACGGCTGTTACAGCTGGGCTCATTATGCTC
CCAGGCGGGCTTCTCAATGGTGCCCTTTCTCCGGTAATGGGACGGCTTTTTGACAAATTTGGTCCACGGCCGCTGTTAAT
TCCAGGCACCATTATCCTTGCAGCGATTGTGTTTAGCTACCGCTATACAACGCCAGGTGTGCCAGTTTGGATCGTCATTG
TCCAACTGGCGATTCTAATGGTTGCTGTCGCCATGATTATGATGCCTACCAATACAAACGGGCTTAACCAACTGCCGGCA
AGACTTTACCCTCACGGAACAGCGATTATGAACACGCTTATGCAAGTCGCTGGCGCAATCGGCGCCGCTTTGTTTATTTC
TGTCATGGAAAACGGCCAACAACGTTATTTGGCTAATTTAGGCATTAGCGAGCCGACCGCTGAACAGACGGTCGAAGCGC
TCCTATACGGTTCCCAACAAAGCTTCTCCACTGGTTTCTTTTTTGCTTGTGCAGCAGTCGTTCTTTCATTTTTTATCAAG
CGCAGCAAACAAACACAAGCCGAAGTAAGTATCCGCCGGTAG

Upstream 100 bases:

>100_bases
AGCAAATACGATTCTATTGCGTTTCGTAATTGGAGGTGGTAAGATAACTTAGCTGAACGCACGCGTTCCGTATGTTTTAT
AAGGAAGGAGAATCTCCAAA

Downstream 100 bases:

>100_bases
AACCGGCGGTTTTAATTTCGCCCTATAAGGGCACTTTACCAACGAAGTCCCTAAAGGGACCTCTGAAGGGACCGCCAACC
GTTTCCTACTTTACTTATCT

Product: lincomycin-resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MAHVSQEPSAAATTETVQVKVLPLLAVLLSGAFVAILSETILNVALNAIMSDFGVASSTAQWLVTGYMLVIGTLIPISAY
FMQRFTTRQLFITAMSLFTLGTLIAGFSPVFAMLLVGRLTQAVGTALMIPLLTNVILAVIPIERRGAAMGMVGLVIMFAP
AIGPTVSGIIVDSLSWRWLFYFITPISLLSLLFGVKVLRNIGETSRPKLDVVSFILSTLGFGGIVFGFSAAGKGDASFTD
PLVYSTLLIGFASLCIFGWRQLKLETPLLDIRVFRYPMFLIGLIVVMLVMMTMFAMMLIMPIYMQGALLFTAVTAGLIML
PGGLLNGALSPVMGRLFDKFGPRPLLIPGTIILAAIVFSYRYTTPGVPVWIVIVQLAILMVAVAMIMMPTNTNGLNQLPA
RLYPHGTAIMNTLMQVAGAIGAALFISVMENGQQRYLANLGISEPTAEQTVEALLYGSQQSFSTGFFFACAAVVLSFFIK
RSKQTQAEVSIRR

Sequences:

>Translated_493_residues
MAHVSQEPSAAATTETVQVKVLPLLAVLLSGAFVAILSETILNVALNAIMSDFGVASSTAQWLVTGYMLVIGTLIPISAY
FMQRFTTRQLFITAMSLFTLGTLIAGFSPVFAMLLVGRLTQAVGTALMIPLLTNVILAVIPIERRGAAMGMVGLVIMFAP
AIGPTVSGIIVDSLSWRWLFYFITPISLLSLLFGVKVLRNIGETSRPKLDVVSFILSTLGFGGIVFGFSAAGKGDASFTD
PLVYSTLLIGFASLCIFGWRQLKLETPLLDIRVFRYPMFLIGLIVVMLVMMTMFAMMLIMPIYMQGALLFTAVTAGLIML
PGGLLNGALSPVMGRLFDKFGPRPLLIPGTIILAAIVFSYRYTTPGVPVWIVIVQLAILMVAVAMIMMPTNTNGLNQLPA
RLYPHGTAIMNTLMQVAGAIGAALFISVMENGQQRYLANLGISEPTAEQTVEALLYGSQQSFSTGFFFACAAVVLSFFIK
RSKQTQAEVSIRR
>Mature_492_residues
AHVSQEPSAAATTETVQVKVLPLLAVLLSGAFVAILSETILNVALNAIMSDFGVASSTAQWLVTGYMLVIGTLIPISAYF
MQRFTTRQLFITAMSLFTLGTLIAGFSPVFAMLLVGRLTQAVGTALMIPLLTNVILAVIPIERRGAAMGMVGLVIMFAPA
IGPTVSGIIVDSLSWRWLFYFITPISLLSLLFGVKVLRNIGETSRPKLDVVSFILSTLGFGGIVFGFSAAGKGDASFTDP
LVYSTLLIGFASLCIFGWRQLKLETPLLDIRVFRYPMFLIGLIVVMLVMMTMFAMMLIMPIYMQGALLFTAVTAGLIMLP
GGLLNGALSPVMGRLFDKFGPRPLLIPGTIILAAIVFSYRYTTPGVPVWIVIVQLAILMVAVAMIMMPTNTNGLNQLPAR
LYPHGTAIMNTLMQVAGAIGAALFISVMENGQQRYLANLGISEPTAEQTVEALLYGSQQSFSTGFFFACAAVVLSFFIKR
SKQTQAEVSIRR

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=443, Percent_Identity=26.410835214447, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1790195, Length=398, Percent_Identity=25.3768844221106, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1788710, Length=411, Percent_Identity=24.330900243309, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081983, Length=403, Percent_Identity=26.7990074441687, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788392, Length=393, Percent_Identity=24.4274809160305, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325455, Length=214, Percent_Identity=24.7663551401869, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53135; Mature: 53004

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCHHHHHCCCC
>Mature Secondary Structure 
AHVSQEPSAAATTETVQVKVLPLLAVLLSGAFVAILSETILNVALNAIMSDFGVASSTA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH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HCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]