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The map label for this gene is cysL [H]

Identifier: 56965027

GI number: 56965027

Start: 3388617

End: 3389501

Strand: Direct

Name: cysL [H]

Synonym: ABC3263

Alternate gene names: 56965027

Gene position: 3388617-3389501 (Clockwise)

Preceding gene: 56965020

Following gene: 56965028

Centisome position: 78.73

GC content: 45.42

Gene sequence:

>885_bases
GTGAACATTCAAAAGCTGGACGTCTTTTTAACGCTTGTCGAAACGAAAAAAATGACCGAAACTGCTCAGCAGTTAAAACT
GTCCGCCCCCACCGTATCGTTTCATATCCGTTCACTTGAAAAAGAATACGGCATAAAGTTGTTCCGTACGAATGCCGGTG
GATACCGGCTAACGCCAGGAGGGGAGATCCTCTACCACTACGCCAAGCAATTGAGCCAAACAAACCAAAAATTAGAACAT
GCCTTGTTTCAATTCAAAAAGGGCGAAGCTGGTTCGCTTCAATTAGGGGCTAGCGGCGTCCCTGCACAACTATTCATGCC
AGAGCTGATCCACAAAATGGCCGAGCGCTACCCGCGCATCAATGTATCACTAGACGTAAAAACCGCACCTGAAATTGAAC
AAAAACTAGTCCGCCAAGAACTTGATTTTGGCTTAATAATGGAAACAAGCAGCCAAGCAAAAGAGCTTGTTTACGAGGCA
TTTGCTTGCGACGAACTAGTACTTGCCTACAGCAAAGACCATCCTTTTTCCAAAGTGGAGACGGTCGCAGAAGTCGACTT
AAGCAAACAACGCCTGCTCGTGCACACACTGTCAAGCTCGACTAAACACTTCTCCAAAACATGGTTAAAAGGCCGTGATG
AAGAGATGGATTTAATTGAGCTTGACTCCGTTTCAACGATTATCAAAATGCTGTCATTCGGCCAAGCCGTCGCCCTCATC
TCCAAGCGCCTAATCGAGAACGAAGACCATCTCGCTTTCACGGAACTAAATGACAACCGATTAAAGCGGCAAATTTATTT
CGTTTATCACCAGCACCTGTGGGAAAACGATGCGATTCTGTTTTTTAAAGAGTCGGTTGCTGCTTTGAAAGAAGTGATTA
CCTGA

Upstream 100 bases:

>100_bases
TGTATAGACATGATTAATCTTCCGTTAACGTTAAATTAAAATAAATCTGCGAAAATCATCGTGTAGTTAGAAAAAATCTA
AAATACGTTAAGGTGATAAA

Downstream 100 bases:

>100_bases
AAAACTAACAGCATTCAGACTGGTAGAAAACGTGAAGAGTGGGCAGGCGAAGGAAGATTCGAATAGAACGGGGTTTATGA
GCAACGATTTTCTTGCGCAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNIQKLDVFLTLVETKKMTETAQQLKLSAPTVSFHIRSLEKEYGIKLFRTNAGGYRLTPGGEILYHYAKQLSQTNQKLEH
ALFQFKKGEAGSLQLGASGVPAQLFMPELIHKMAERYPRINVSLDVKTAPEIEQKLVRQELDFGLIMETSSQAKELVYEA
FACDELVLAYSKDHPFSKVETVAEVDLSKQRLLVHTLSSSTKHFSKTWLKGRDEEMDLIELDSVSTIIKMLSFGQAVALI
SKRLIENEDHLAFTELNDNRLKRQIYFVYHQHLWENDAILFFKESVAALKEVIT

Sequences:

>Translated_294_residues
MNIQKLDVFLTLVETKKMTETAQQLKLSAPTVSFHIRSLEKEYGIKLFRTNAGGYRLTPGGEILYHYAKQLSQTNQKLEH
ALFQFKKGEAGSLQLGASGVPAQLFMPELIHKMAERYPRINVSLDVKTAPEIEQKLVRQELDFGLIMETSSQAKELVYEA
FACDELVLAYSKDHPFSKVETVAEVDLSKQRLLVHTLSSSTKHFSKTWLKGRDEEMDLIELDSVSTIIKMLSFGQAVALI
SKRLIENEDHLAFTELNDNRLKRQIYFVYHQHLWENDAILFFKESVAALKEVIT
>Mature_294_residues
MNIQKLDVFLTLVETKKMTETAQQLKLSAPTVSFHIRSLEKEYGIKLFRTNAGGYRLTPGGEILYHYAKQLSQTNQKLEH
ALFQFKKGEAGSLQLGASGVPAQLFMPELIHKMAERYPRINVSLDVKTAPEIEQKLVRQELDFGLIMETSSQAKELVYEA
FACDELVLAYSKDHPFSKVETVAEVDLSKQRLLVHTLSSSTKHFSKTWLKGRDEEMDLIELDSVSTIIKMLSFGQAVALI
SKRLIENEDHLAFTELNDNRLKRQIYFVYHQHLWENDAILFFKESVAALKEVIT

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=283, Percent_Identity=24.7349823321555, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790399, Length=276, Percent_Identity=22.8260869565217, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788481, Length=286, Percent_Identity=21.6783216783217, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=23.6947791164659, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789204, Length=247, Percent_Identity=22.2672064777328, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693105, Length=240, Percent_Identity=22.0833333333333, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI2367136, Length=271, Percent_Identity=22.5092250922509, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787530, Length=200, Percent_Identity=22.5, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789639, Length=287, Percent_Identity=22.9965156794425, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33625; Mature: 33625

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQKLDVFLTLVETKKMTETAQQLKLSAPTVSFHIRSLEKEYGIKLFRTNAGGYRLTPG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEECCCCEEECCC
GEILYHYAKQLSQTNQKLEHALFQFKKGEAGSLQLGASGVPAQLFMPELIHKMAERYPRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCE
NVSLDVKTAPEIEQKLVRQELDFGLIMETSSQAKELVYEAFACDELVLAYSKDHPFSKVE
EEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHEECCCCCHHHHH
TVAEVDLSKQRLLVHTLSSSTKHFSKTWLKGRDEEMDLIELDSVSTIIKMLSFGQAVALI
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCHHHHHH
SKRLIENEDHLAFTELNDNRLKRQIYFVYHQHLWENDAILFFKESVAALKEVIT
HHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MNIQKLDVFLTLVETKKMTETAQQLKLSAPTVSFHIRSLEKEYGIKLFRTNAGGYRLTPG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEECCCCEEECCC
GEILYHYAKQLSQTNQKLEHALFQFKKGEAGSLQLGASGVPAQLFMPELIHKMAERYPRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCE
NVSLDVKTAPEIEQKLVRQELDFGLIMETSSQAKELVYEAFACDELVLAYSKDHPFSKVE
EEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHEECCCCCHHHHH
TVAEVDLSKQRLLVHTLSSSTKHFSKTWLKGRDEEMDLIELDSVSTIIKMLSFGQAVALI
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCHHHHHH
SKRLIENEDHLAFTELNDNRLKRQIYFVYHQHLWENDAILFFKESVAALKEVIT
HHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]