| Definition | Bacillus clausii KSM-K16, complete genome. |
|---|---|
| Accession | NC_006582 |
| Length | 4,303,871 |
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The map label for this gene is pbpA [H]
Identifier: 56964523
GI number: 56964523
Start: 2892047
End: 2894368
Strand: Direct
Name: pbpA [H]
Synonym: ABC2759
Alternate gene names: 56964523
Gene position: 2892047-2894368 (Clockwise)
Preceding gene: 56964521
Following gene: 56964525
Centisome position: 67.2
GC content: 46.99
Gene sequence:
>2322_bases ATGAAGCACTTTCTTTCGAAGTGGAAGAACAACTGGGATGCCTTTATAGCAAAGCTAGATGACATTCAGTTCTTCCGGAA AGTCGGCATTACATACCAAGTATTATGGAATTTATTCCTTATTGTATTCATTATTGGCTTTTTAGGATTTATGTTCGCTA GCGGAACCGCCGCAGGTTACTTTGCTTCCCTCGTAGTCGATGAAAAAACATATTCAGAAGAAGACATGCGCACATTCGTA GGCTCGCTCTCAAAAACAAGCGACATTTACCTTGCTGGCGGCGTTTACTTAGGAAAAATCCGCAGTGAAGTAGACCGGGA AATTGTCTCCCTTGATGACGTGGCCGATGAAGTAAAATGGGCGATCATTGCTACAGAAGACGAACATTTTTACGAGCATA ATGGCATCGTCCCAAAAGCGTTGCTACGGGCAACGATGCAAGAGCTGACTAGTTCGTCTGTTCAGACAGGCGGCAGCACA TTGACACAACAATTAATAAAGCAACAAATTTTATCCGATGAAGTATCGTTCGACCGCAAAGCAACAGAAATTATGCTCGC GCTTAGGCTCGAACAGTTTATGACAAAAGATGAAATATTGGAAGCCTACTTAAACGTCGTTCCGTTTGGCCGGAATGCCT CGGGCCGAAATGTTGCTGGCATTGAAGCGGCAGCACAAGGGGTCTTTGGTGTTTCGGCAAGCGAGTTAAACTTGCCACAA GCAGCTTACTTAGCTGGCTTGCCGCAAAGCCCGTATGGCTACACGCCTTTTACAAACGACCAACAGCTTAAAGATCAAGA CGGTTTGCAGCCTGGGTTGAACCGTTACCAAACGGTTTTAAGCCGCATGTACGAAAAAGAGTACATCACAAAAGAGCAAT ACGAGGATGCAAAAGACTACGACCTTACTGCTGATTTTACAACAAGCGCGCCTGACCCAATGGAAGAACGGCCGCGGCTT ACTGAAGAAATTATGAACCGGGCAACGGCAGTGCTCGTACAACAAAAAATCGATAACCGCGATGGTTGGGAGAAATTAAG CGACACTGCTAAACAAGTTGAACGCAATCAGTTTAAAGCGGAAGTCGAAAACGCGATTATCAATGGTGGTTACAACATCA TTACGACCATTGATAAAGATTTGTATGATGCCATGAACGAGGCAGCTCAACAATCTGACGCGTATTTTGGTGCCGACAAA CAAGGGCAACGGGAAGAAGTCGGCTCTGTTTTAATCGAAAATAAAACTGGAAAAATCCTTAGCTTTGTCGGTGGTCGTGA AGAAAGCTTAGATCATCTGTATAACCACGCTACACGTGCTTATCGCCAAGCAGGCTCGACGAGCAAACCTGTTCTTGCTT ATGCGGGCGCGATTGAAGCAGGAGTAACCCAACCAGGGCTTGTCATTCCTGATGAACAAGCGTTTTACAAAGCTGGTGGG AGGGAAATTAGAAACTTTGACCACGCTCACAAAGGCAATCTCACTGTGCGGGAATCACTGGCACAATCACGCAACGTGCC CGCTGTAGAGGCGTGGTGGCATGTCCCTGATGACTTAAAAGTAAAACTCCGCGACGCAGCCGGCTTTAATCCAGACATGC ACGGCGTGAATGAAGCAGTCGCTATCGGTGGCGGCAATGTTACCGTTGAACAAAATGTCAGCGCCTTTAGCGCTTTCGGC AACAATGGCGTCCGGATGGAACCCTATATGATCGAACGGATCGAAACGGATTCCGGCGAAGTCATTTATGAGCATCCTGC TAGTAAGCATACAATGTTTAGCCCACAAACAAACTATTTGGTCGCCGATATGATGCGTGATGTGATGTACAGCGCAAATG GCACTGGCCGCACAGCAGCCAACAAGTTGCAGTTTCAAGCCGACTGGGCTGGAAAAACAGGCACGACCAACAGAGACTAT GATTCATGGTTTATTGCCGTCAACCCTGAGGTGACATTAGGCGTATGGAATGGTTATAGCGGTGACAAAACAATTAGTTT ACGCAAACGCGTTGGCGGGCTCGGCACAGGGGCGCGGACACAGTCCATTTGGGCCAATCTTGCTAATGCTGCTTATGGTG TTGACCCGGATCTAATGACAGCAGAAGGCAAACGCTTTGAGCAGCCTGATGGCATTGTCGAAAAACGAGTCTGTGGCTTA ACAGGCGGCCCACCAACAAAAGCATGCGAAGAACAAGGCTTAGTCACTACCGACCTATACAGAAGCGACATGATCGATCA TATCGAGGGGCTAGCCCCGTTTCAAGCGAACTTTAAACCGAATGCTCAACCATCTAATGAAAAAGAAGATGATGACGATT AA
Upstream 100 bases:
>100_bases TTCTAGTTTTACCATACGGTGTGTACACTGTCAACATAGCGAACACACGTATGCGCATCACGCAATTTATGATATACTAA GACGACTAAGGGGGGAGTCG
Downstream 100 bases:
>100_bases AGAAGCGTGCCTCTAAAGGTAGAGGCACGCTTCTTTGCGTTAATCTTCCATTGTCGATAAGTCGCCTGTCGGCAAATCCA ATTCCCACGCTTTTAATACG
Product: penicillin-binding protein 1A
Products: NA
Alternate protein names: PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 773; Mature: 773
Protein sequence:
>773_residues MKHFLSKWKNNWDAFIAKLDDIQFFRKVGITYQVLWNLFLIVFIIGFLGFMFASGTAAGYFASLVVDEKTYSEEDMRTFV GSLSKTSDIYLAGGVYLGKIRSEVDREIVSLDDVADEVKWAIIATEDEHFYEHNGIVPKALLRATMQELTSSSVQTGGST LTQQLIKQQILSDEVSFDRKATEIMLALRLEQFMTKDEILEAYLNVVPFGRNASGRNVAGIEAAAQGVFGVSASELNLPQ AAYLAGLPQSPYGYTPFTNDQQLKDQDGLQPGLNRYQTVLSRMYEKEYITKEQYEDAKDYDLTADFTTSAPDPMEERPRL TEEIMNRATAVLVQQKIDNRDGWEKLSDTAKQVERNQFKAEVENAIINGGYNIITTIDKDLYDAMNEAAQQSDAYFGADK QGQREEVGSVLIENKTGKILSFVGGREESLDHLYNHATRAYRQAGSTSKPVLAYAGAIEAGVTQPGLVIPDEQAFYKAGG REIRNFDHAHKGNLTVRESLAQSRNVPAVEAWWHVPDDLKVKLRDAAGFNPDMHGVNEAVAIGGGNVTVEQNVSAFSAFG NNGVRMEPYMIERIETDSGEVIYEHPASKHTMFSPQTNYLVADMMRDVMYSANGTGRTAANKLQFQADWAGKTGTTNRDY DSWFIAVNPEVTLGVWNGYSGDKTISLRKRVGGLGTGARTQSIWANLANAAYGVDPDLMTAEGKRFEQPDGIVEKRVCGL TGGPPTKACEEQGLVTTDLYRSDMIDHIEGLAPFQANFKPNAQPSNEKEDDDD
Sequences:
>Translated_773_residues MKHFLSKWKNNWDAFIAKLDDIQFFRKVGITYQVLWNLFLIVFIIGFLGFMFASGTAAGYFASLVVDEKTYSEEDMRTFV GSLSKTSDIYLAGGVYLGKIRSEVDREIVSLDDVADEVKWAIIATEDEHFYEHNGIVPKALLRATMQELTSSSVQTGGST LTQQLIKQQILSDEVSFDRKATEIMLALRLEQFMTKDEILEAYLNVVPFGRNASGRNVAGIEAAAQGVFGVSASELNLPQ AAYLAGLPQSPYGYTPFTNDQQLKDQDGLQPGLNRYQTVLSRMYEKEYITKEQYEDAKDYDLTADFTTSAPDPMEERPRL TEEIMNRATAVLVQQKIDNRDGWEKLSDTAKQVERNQFKAEVENAIINGGYNIITTIDKDLYDAMNEAAQQSDAYFGADK QGQREEVGSVLIENKTGKILSFVGGREESLDHLYNHATRAYRQAGSTSKPVLAYAGAIEAGVTQPGLVIPDEQAFYKAGG REIRNFDHAHKGNLTVRESLAQSRNVPAVEAWWHVPDDLKVKLRDAAGFNPDMHGVNEAVAIGGGNVTVEQNVSAFSAFG NNGVRMEPYMIERIETDSGEVIYEHPASKHTMFSPQTNYLVADMMRDVMYSANGTGRTAANKLQFQADWAGKTGTTNRDY DSWFIAVNPEVTLGVWNGYSGDKTISLRKRVGGLGTGARTQSIWANLANAAYGVDPDLMTAEGKRFEQPDGIVEKRVCGL TGGPPTKACEEQGLVTTDLYRSDMIDHIEGLAPFQANFKPNAQPSNEKEDDDD >Mature_773_residues MKHFLSKWKNNWDAFIAKLDDIQFFRKVGITYQVLWNLFLIVFIIGFLGFMFASGTAAGYFASLVVDEKTYSEEDMRTFV GSLSKTSDIYLAGGVYLGKIRSEVDREIVSLDDVADEVKWAIIATEDEHFYEHNGIVPKALLRATMQELTSSSVQTGGST LTQQLIKQQILSDEVSFDRKATEIMLALRLEQFMTKDEILEAYLNVVPFGRNASGRNVAGIEAAAQGVFGVSASELNLPQ AAYLAGLPQSPYGYTPFTNDQQLKDQDGLQPGLNRYQTVLSRMYEKEYITKEQYEDAKDYDLTADFTTSAPDPMEERPRL TEEIMNRATAVLVQQKIDNRDGWEKLSDTAKQVERNQFKAEVENAIINGGYNIITTIDKDLYDAMNEAAQQSDAYFGADK QGQREEVGSVLIENKTGKILSFVGGREESLDHLYNHATRAYRQAGSTSKPVLAYAGAIEAGVTQPGLVIPDEQAFYKAGG REIRNFDHAHKGNLTVRESLAQSRNVPAVEAWWHVPDDLKVKLRDAAGFNPDMHGVNEAVAIGGGNVTVEQNVSAFSAFG NNGVRMEPYMIERIETDSGEVIYEHPASKHTMFSPQTNYLVADMMRDVMYSANGTGRTAANKLQFQADWAGKTGTTNRDY DSWFIAVNPEVTLGVWNGYSGDKTISLRKRVGGLGTGARTQSIWANLANAAYGVDPDLMTAEGKRFEQPDGIVEKRVCGL TGGPPTKACEEQGLVTTDLYRSDMIDHIEGLAPFQANFKPNAQPSNEKEDDDD
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786343, Length=578, Percent_Identity=27.3356401384083, Blast_Score=152, Evalue=1e-37, Organism=Escherichia coli, GI1788867, Length=567, Percent_Identity=24.8677248677249, Blast_Score=146, Evalue=5e-36, Organism=Escherichia coli, GI87082258, Length=302, Percent_Identity=33.7748344370861, Blast_Score=145, Evalue=8e-36, Organism=Escherichia coli, GI1789601, Length=190, Percent_Identity=33.6842105263158, Blast_Score=79, Evalue=1e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.1.129; 3.4.-.- [C]
Molecular weight: Translated: 85721; Mature: 85721
Theoretical pI: Translated: 4.61; Mature: 4.61
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKHFLSKWKNNWDAFIAKLDDIQFFRKVGITYQVLWNLFLIVFIIGFLGFMFASGTAAGY CCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH FASLVVDEKTYSEEDMRTFVGSLSKTSDIYLAGGVYLGKIRSEVDREIVSLDDVADEVKW HHHHEECCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCEE AIIATEDEHFYEHNGIVPKALLRATMQELTSSSVQTGGSTLTQQLIKQQILSDEVSFDRK EEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHH ATEIMLALRLEQFMTKDEILEAYLNVVPFGRNASGRNVAGIEAAAQGVFGVSASELNLPQ HHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCHHHCCCCH AAYLAGLPQSPYGYTPFTNDQQLKDQDGLQPGLNRYQTVLSRMYEKEYITKEQYEDAKDY HHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCC DLTADFTTSAPDPMEERPRLTEEIMNRATAVLVQQKIDNRDGWEKLSDTAKQVERNQFKA CCEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH EVENAIINGGYNIITTIDKDLYDAMNEAAQQSDAYFGADKQGQREEVGSVLIENKTGKIL HHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEEECCCCCCH SFVGGREESLDHLYNHATRAYRQAGSTSKPVLAYAGAIEAGVTQPGLVIPDEQAFYKAGG HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCCCEECCCCHHHHHCCC REIRNFDHAHKGNLTVRESLAQSRNVPAVEAWWHVPDDLKVKLRDAAGFNPDMHGVNEAV HHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCEE AIGGGNVTVEQNVSAFSAFGNNGVRMEPYMIERIETDSGEVIYEHPASKHTMFSPQTNYL EECCCCEEEECCCHHHHHHCCCCCEECCHHHEEEECCCCCEEEECCCCCCCCCCCCCCHH VADMMRDVMYSANGTGRTAANKLQFQADWAGKTGTTNRDYDSWFIAVNPEVTLGVWNGYS HHHHHHHHHHCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCEEEEECCEEEEEEECCCC GDKTISLRKRVGGLGTGARTQSIWANLANAAYGVDPDLMTAEGKRFEQPDGIVEKRVCGL CCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCC TGGPPTKACEEQGLVTTDLYRSDMIDHIEGLAPFQANFKPNAQPSNEKEDDDD CCCCCCHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC >Mature Secondary Structure MKHFLSKWKNNWDAFIAKLDDIQFFRKVGITYQVLWNLFLIVFIIGFLGFMFASGTAAGY CCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH FASLVVDEKTYSEEDMRTFVGSLSKTSDIYLAGGVYLGKIRSEVDREIVSLDDVADEVKW HHHHEECCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCEE AIIATEDEHFYEHNGIVPKALLRATMQELTSSSVQTGGSTLTQQLIKQQILSDEVSFDRK EEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHH ATEIMLALRLEQFMTKDEILEAYLNVVPFGRNASGRNVAGIEAAAQGVFGVSASELNLPQ HHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCHHHCCCCH AAYLAGLPQSPYGYTPFTNDQQLKDQDGLQPGLNRYQTVLSRMYEKEYITKEQYEDAKDY HHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCC DLTADFTTSAPDPMEERPRLTEEIMNRATAVLVQQKIDNRDGWEKLSDTAKQVERNQFKA CCEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH EVENAIINGGYNIITTIDKDLYDAMNEAAQQSDAYFGADKQGQREEVGSVLIENKTGKIL HHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEEECCCCCCH SFVGGREESLDHLYNHATRAYRQAGSTSKPVLAYAGAIEAGVTQPGLVIPDEQAFYKAGG HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCCCEECCCCHHHHHCCC REIRNFDHAHKGNLTVRESLAQSRNVPAVEAWWHVPDDLKVKLRDAAGFNPDMHGVNEAV HHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCEE AIGGGNVTVEQNVSAFSAFGNNGVRMEPYMIERIETDSGEVIYEHPASKHTMFSPQTNYL EECCCCEEEECCCHHHHHHCCCCCEECCHHHEEEECCCCCEEEECCCCCCCCCCCCCCHH VADMMRDVMYSANGTGRTAANKLQFQADWAGKTGTTNRDYDSWFIAVNPEVTLGVWNGYS HHHHHHHHHHCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCEEEEECCEEEEEEECCCC GDKTISLRKRVGGLGTGARTQSIWANLANAAYGVDPDLMTAEGKRFEQPDGIVEKRVCGL CCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCC TGGPPTKACEEQGLVTTDLYRSDMIDHIEGLAPFQANFKPNAQPSNEKEDDDD CCCCCCHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA