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The map label for this gene is prfC [H]

Identifier: 56964314

GI number: 56964314

Start: 2668552

End: 2670159

Strand: Direct

Name: prfC [H]

Synonym: ABC2549

Alternate gene names: 56964314

Gene position: 2668552-2670159 (Clockwise)

Preceding gene: 56964309

Following gene: 56964325

Centisome position: 62.0

GC content: 44.65

Gene sequence:

>1608_bases
ATGAACCCAGCATTAAAAGAAGAAATTTTAGCAAGGCGTACCTTTGCGATTATCTCCCACCCCGATGCCGGAAAAACAAC
ATTAACTGAAAAGTTGCTTCTTTTTGGCGGTGCCATACGGGAAGCAGGAACAGTGAAAGGGCGCAAAAATAGCAAACACG
CAATGAGTGATTGGATGGAAATTGAAAAACAGCGTGGCATCTCGGTTACAAGCTCTGTGCTTGAATTCCAGTACAATGGC
TTTCATGTCAATATTCTCGATACACCTGGCCACGAAGATTTTTCGGAAGATACGTACCGCACATTGACTGCTGCTGATTC
TGCCGCAATGTTAATTGACGCTGCCAAAGGAGTTGAAGCACAGACGAAAAAGCTTTTTAAAGTCTGCCGCCTCCGCAATA
TTCCGATTTTCACGTTCATTAACAAACTTGACCGTCAAGGGCGTGATCCATTTGAATTAATGGAAGAACTAGAAAATCTC
CTTGGCATTCGTTCATACCCAATGGGCTGGCCGATTGGCATGGGCCAATCATTTAAAGCTGTTTATGACCGTCACCAGCA
AAAACTAGAATTATACAATCCTGACAATCCAAAGGCGATTGATACGATTCAACTAAGCGGGCCAGACGATCCGAAGTTGG
ATGAAGCAATTGGCGATGAACAACTGGTCCGTCAGTTCCGGGAAGAGCTAGAACTCCTTGATGTCGCTGGTGATGAATAT
GACAAAGAACGGATTGACAAAGGCGAACTGACACCTGTATTTTTCGGTTCGGCGATTTCAAGCTTCGGCGTACAAACGTT
TCTGGACCATTTTTTAAAGCTTTCTCCTGCGCCAGCTTCCCGGGAGAGCTCGATTGGGACGATTGACCCCCTTTCTCGGG
AACATTTTTCCGGTTTTATTTTTAAAGTGCAAGCCAATATGAATGCTAAACACCGGGACCGCGTCGCATTTTTGCGGATC
ACTTCTGGTACATTTAAACGGGGAATGAACGTCAAGCATGTCCGGACCAACAAACCGTTAAAACTCTCGCAGCCAACACA
ATTTTTAGCACAAAACCGCAGCATTGTTGATGAAGCGTATGCTGGCGACATTATCGGTTTATTTGACCCAGGTACATTTC
GAATCGGCGACACGCTTGTTGATGGTGAATCGTTTGAGTTTAATGAAATGCCTCATTTCTCACCAGAGCACTTCGCAAGC
ATTACGGTGAAAGAAGCTTTAAAGCACAAATCATATGAGAAAGGCTTGCAACAATTAACGGAAGAGGGCGCGATCCAGTT
ATTTAAAACCTATAATAAGTACGTTGAACAACAAATTGTCGGCGTGGTTGGCGTACTCCAATTTGAAGTGCTTGAACACC
GTCTCCACGCTGAATACAATGTCGATATCCAGATGGAACGGTTGCCGTTTTCTTTTGCCCGTTGGATCAGCGGCGGCGAA
GAAGAGATGAAACGATTTAAAGAGAAACAGCGCAACCTCGTCACCGACAGAGAAGGCCGGATTGTTGCTTTGTTTGAGAA
TGAATTCCAAATGAGGACAGCGCAAGATAAATATCCAGGCCTCCATTTTTACGAAAACTCCTTTTACCAAGACAGTATGA
CCGAGTAA

Upstream 100 bases:

>100_bases
GTTGCTCGATGGCAAGGATGGACCGATTTGCTTTTCAAAGATGAAAGCAGGGCCGTTTCTTGCTATAATGGAAAAATCGA
TTTGGAAATGAGGTTGATTC

Downstream 100 bases:

>100_bases
AACACGAAAAAACGGCAGTTGTGAAAAATGCAACTGCCGTTTTTCGTCATCTTTGTCACTGATTTGTGTTTGGATGCTTA
GCTGCCATGCTCTTTGTTTC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MNPALKEEILARRTFAIISHPDAGKTTLTEKLLLFGGAIREAGTVKGRKNSKHAMSDWMEIEKQRGISVTSSVLEFQYNG
FHVNILDTPGHEDFSEDTYRTLTAADSAAMLIDAAKGVEAQTKKLFKVCRLRNIPIFTFINKLDRQGRDPFELMEELENL
LGIRSYPMGWPIGMGQSFKAVYDRHQQKLELYNPDNPKAIDTIQLSGPDDPKLDEAIGDEQLVRQFREELELLDVAGDEY
DKERIDKGELTPVFFGSAISSFGVQTFLDHFLKLSPAPASRESSIGTIDPLSREHFSGFIFKVQANMNAKHRDRVAFLRI
TSGTFKRGMNVKHVRTNKPLKLSQPTQFLAQNRSIVDEAYAGDIIGLFDPGTFRIGDTLVDGESFEFNEMPHFSPEHFAS
ITVKEALKHKSYEKGLQQLTEEGAIQLFKTYNKYVEQQIVGVVGVLQFEVLEHRLHAEYNVDIQMERLPFSFARWISGGE
EEMKRFKEKQRNLVTDREGRIVALFENEFQMRTAQDKYPGLHFYENSFYQDSMTE

Sequences:

>Translated_535_residues
MNPALKEEILARRTFAIISHPDAGKTTLTEKLLLFGGAIREAGTVKGRKNSKHAMSDWMEIEKQRGISVTSSVLEFQYNG
FHVNILDTPGHEDFSEDTYRTLTAADSAAMLIDAAKGVEAQTKKLFKVCRLRNIPIFTFINKLDRQGRDPFELMEELENL
LGIRSYPMGWPIGMGQSFKAVYDRHQQKLELYNPDNPKAIDTIQLSGPDDPKLDEAIGDEQLVRQFREELELLDVAGDEY
DKERIDKGELTPVFFGSAISSFGVQTFLDHFLKLSPAPASRESSIGTIDPLSREHFSGFIFKVQANMNAKHRDRVAFLRI
TSGTFKRGMNVKHVRTNKPLKLSQPTQFLAQNRSIVDEAYAGDIIGLFDPGTFRIGDTLVDGESFEFNEMPHFSPEHFAS
ITVKEALKHKSYEKGLQQLTEEGAIQLFKTYNKYVEQQIVGVVGVLQFEVLEHRLHAEYNVDIQMERLPFSFARWISGGE
EEMKRFKEKQRNLVTDREGRIVALFENEFQMRTAQDKYPGLHFYENSFYQDSMTE
>Mature_535_residues
MNPALKEEILARRTFAIISHPDAGKTTLTEKLLLFGGAIREAGTVKGRKNSKHAMSDWMEIEKQRGISVTSSVLEFQYNG
FHVNILDTPGHEDFSEDTYRTLTAADSAAMLIDAAKGVEAQTKKLFKVCRLRNIPIFTFINKLDRQGRDPFELMEELENL
LGIRSYPMGWPIGMGQSFKAVYDRHQQKLELYNPDNPKAIDTIQLSGPDDPKLDEAIGDEQLVRQFREELELLDVAGDEY
DKERIDKGELTPVFFGSAISSFGVQTFLDHFLKLSPAPASRESSIGTIDPLSREHFSGFIFKVQANMNAKHRDRVAFLRI
TSGTFKRGMNVKHVRTNKPLKLSQPTQFLAQNRSIVDEAYAGDIIGLFDPGTFRIGDTLVDGESFEFNEMPHFSPEHFAS
ITVKEALKHKSYEKGLQQLTEEGAIQLFKTYNKYVEQQIVGVVGVLQFEVLEHRLHAEYNVDIQMERLPFSFARWISGGE
EEMKRFKEKQRNLVTDREGRIVALFENEFQMRTAQDKYPGLHFYENSFYQDSMTE

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=495, Percent_Identity=29.0909090909091, Blast_Score=186, Evalue=7e-47,
Organism=Homo sapiens, GI19923640, Length=534, Percent_Identity=25.4681647940075, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=28.6445012787724, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI25306287, Length=289, Percent_Identity=30.1038062283737, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=33.1210191082803, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=32.8467153284672, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=27.5167785234899, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790835, Length=531, Percent_Identity=50.2824858757062, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1789738, Length=526, Percent_Identity=27.9467680608365, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=31.4102564102564, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI48994988, Length=148, Percent_Identity=33.7837837837838, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=493, Percent_Identity=25.5578093306288, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=494, Percent_Identity=25.3036437246964, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=148, Percent_Identity=37.1621621621622, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=29.8136645962733, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.0751879699248, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.0751879699248, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=490, Percent_Identity=27.5510204081633, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322359, Length=530, Percent_Identity=27.1698113207547, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=27.8481012658228, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=27.8481012658228, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=153, Percent_Identity=28.1045751633987, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24582462, Length=493, Percent_Identity=27.3833671399594, Blast_Score=188, Evalue=8e-48,
Organism=Drosophila melanogaster, GI221458488, Length=514, Percent_Identity=25.875486381323, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI78706572, Length=156, Percent_Identity=35.8974358974359, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=28.7581699346405, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=28.7581699346405, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=28.7581699346405, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 61093; Mature: 61093

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPALKEEILARRTFAIISHPDAGKTTLTEKLLLFGGAIREAGTVKGRKNSKHAMSDWME
CCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
IEKQRGISVTSSVLEFQYNGFHVNILDTPGHEDFSEDTYRTLTAADSAAMLIDAAKGVEA
HHHHCCCHHHHHHHHEEECCEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCHH
QTKKLFKVCRLRNIPIFTFINKLDRQGRDPFELMEELENLLGIRSYPMGWPIGMGQSFKA
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
VYDRHQQKLELYNPDNPKAIDTIQLSGPDDPKLDEAIGDEQLVRQFREELELLDVAGDEY
HHHHHHHHEECCCCCCCCEEEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCH
DKERIDKGELTPVFFGSAISSFGVQTFLDHFLKLSPAPASRESSIGTIDPLSREHFSGFI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCEE
FKVQANMNAKHRDRVAFLRITSGTFKRGMNVKHVRTNKPLKLSQPTQFLAQNRSIVDEAY
EEEEECCCCCCCCCEEEEEEECCHHHHCCCEEEEECCCCEECCCCHHHHHCCCHHHHHHH
AGDIIGLFDPGTFRIGDTLVDGESFEFNEMPHFSPEHFASITVKEALKHKSYEKGLQQLT
CCCEEEEECCCCEEECCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGAIQLFKTYNKYVEQQIVGVVGVLQFEVLEHRLHAEYNVDIQMERLPFSFARWISGGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCH
EEMKRFKEKQRNLVTDREGRIVALFENEFQMRTAQDKYPGLHFYENSFYQDSMTE
HHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MNPALKEEILARRTFAIISHPDAGKTTLTEKLLLFGGAIREAGTVKGRKNSKHAMSDWME
CCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
IEKQRGISVTSSVLEFQYNGFHVNILDTPGHEDFSEDTYRTLTAADSAAMLIDAAKGVEA
HHHHCCCHHHHHHHHEEECCEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCHH
QTKKLFKVCRLRNIPIFTFINKLDRQGRDPFELMEELENLLGIRSYPMGWPIGMGQSFKA
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
VYDRHQQKLELYNPDNPKAIDTIQLSGPDDPKLDEAIGDEQLVRQFREELELLDVAGDEY
HHHHHHHHEECCCCCCCCEEEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCH
DKERIDKGELTPVFFGSAISSFGVQTFLDHFLKLSPAPASRESSIGTIDPLSREHFSGFI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCEE
FKVQANMNAKHRDRVAFLRITSGTFKRGMNVKHVRTNKPLKLSQPTQFLAQNRSIVDEAY
EEEEECCCCCCCCCEEEEEEECCHHHHCCCEEEEECCCCEECCCCHHHHHCCCHHHHHHH
AGDIIGLFDPGTFRIGDTLVDGESFEFNEMPHFSPEHFASITVKEALKHKSYEKGLQQLT
CCCEEEEECCCCEEECCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGAIQLFKTYNKYVEQQIVGVVGVLQFEVLEHRLHAEYNVDIQMERLPFSFARWISGGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCH
EEMKRFKEKQRNLVTDREGRIVALFENEFQMRTAQDKYPGLHFYENSFYQDSMTE
HHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA