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The map label for this gene is cca [H]

Identifier: 56964308

GI number: 56964308

Start: 2660205

End: 2662301

Strand: Direct

Name: cca [H]

Synonym: ABC2543

Alternate gene names: 56964308

Gene position: 2660205-2662301 (Clockwise)

Preceding gene: 56964307

Following gene: 56964309

Centisome position: 61.81

GC content: 48.31

Gene sequence:

>2097_bases
ATGAAAGCAATTGTCAGCCTCAACCATCTTGATTTTCATGGCCTTGCTATTCTTGCAGCGGCCCAAAAAATTCATCCGGA
TGCCATTGTTGTGCTACCGCCTATTTACCAACATGCTGTCAAACGATTTTTGGACGACTATAAAACCGATTTTTCCTTTC
AGCATGATGGCAAGCTTTCATGGAACGAAGTAGATGAAATTGTATATGTCGATTGGGAAGATGAAAAGCAGGAAAGCCTT
TATCGCTCGTTGCCCGCTTCTGCTGTTGAGACGAATTTGTGGCGAACGATCAAAGCGACTAAAAGAGGCGTGCCGATCAC
TACCCTCCTTTATGAGATGAAACGAAAACAAATTTCGGTAACAGCTATAGAAGCCACGCTTTTTGCTCTAGGTTTATATA
GCAGCACCCATCATTTAACGCTCCCTTCAACCACGGCTAGTGATGGCGATGCCTGTGCCTACTTACTTGAGAAAGGCGCT
GATTTGCGCGTAGTCAACGACTATTTGCAACAAGCGCCACTGGCTGAAAAAATCGCTTCTGTAATGAGTAAGCCTGTTAT
AACTGTACAAACATCGCAATTGATTGATGAAGTGTGGCAAACACTGCTTCGTTCAGGACATAGCGGTTTTCCCGTTGTCG
ATGAGACGGGGGCACTAGCAGGCGTTATTACGCGTATGGACCTCGCCAAGGCACGCCAGTTTGGCATGGGAGAAGCTCAA
GTCACCGAAGTGATGAGCATGCCTAACATTACGCTTCGGGCAAACGATTCGATTGATGCCGCTTGTGCCCACCTTGCTTA
CAATCAAGTCGGCCGTCTCCCTGTCGTCGGCGACAACAACGAACCGATTGGCATTGTGACTCGAACAGACATCGTCCGTT
CGCTTTACCCGAATAAGCATGCCGTTGCCCCTTCTGAGCTCGCGTCTTATTTTGGGAAGCAAACATGTTCCTTTCTGCAA
AAGATTGGTGCTTTCGCCGACGAACTCCAAGTACCTGTCTATTTAGTTGGCGGCCTTGTGCGCGACTTTTTCTTAAAGCG
CCCACATAAAGATATTGACTTAGTCGTTGAAGGGGATGGAATTGCCTTTGCCAAGCAACTGGCAACCGCATTTGGGGGAA
GCGTGCGCAGCCATGAGTCATTTGGCACTGCTACGTGGGCCAACGAACAAGACATTGACATTGTGACGTGTAGAAAAGAG
TTTTATTTGCAAAAAGGGGCATTGCCTACCGTGCGCCCGGCTTCGATTTACGAAGACCTAGCAAGGCGGGATTTCTCTAT
AAATGCGATGGCAATCCAAATCAATCGATCCTCATTCGGGAACGTGCTCGATGTGTTTCAGGGAAAACAAGCGCTTATCG
ACAAACACATTCGCATTCTCCATCCGCTCAGTTTCATAGAAGACCCGACCCGGCTTTTCCGCGCTGTCCGTTTTGGCCTG
CGCCTAAACTTCAGCCTCTCCTCAGAGACGATACACCAAGCAACTAAAACAGGTGCTGCTCTGCACCACATTAGTGCTAA
ACGCTTACGGCAGGAACTTGATTTGCTAGCTAATGAAGGCGTGCTTTTTGAAGGCTTTCGCCAATTGGCTGACCTCCATG
TCTGGACAACGTTGTTTGGTTCCCGTTTTTCCGAGCGAGCCTGGCGGCATATGGCCAACTTGCAGCAACATGGTTTGAAT
GATGGCATGTTCTTTTTACTGGCTGGCGCTGTTGATTGCACTCGTCTTGACGTCGCAAGTCGTTACGCTCTTACAAAGCA
AGAAAAACGCTTGGTAGAAGAAACAAGCTTGCCCGTTTGGCAACAACTTGCGCCGACAGCCTCACTCGGAGAAGCACATC
GCGACTTGGCGCGAATTTCAAGCGAAATCGTTCGCTTTTATAGCGAGGCCGAACTTCCGCTAAGCCCGCTCTTACGTCGT
TATGCCGAAAAACGCACACAGTTTAAGCCGCTTTTGACAGGAGCTGATTTGCTCAAAGCGGGTTACCAACCAGGTCCTGT
ATTTACACAATGGTTGCTCGAAATCGAATGCCTACAACTTGACGGGCATATTGCTACAAAAGACCAAGCCCTTGCTTGGA
TCGCTGAGAAAACATAA

Upstream 100 bases:

>100_bases
GGCTGGCCATGGATTACTTCTGTTAAAAAGCAGCGTCTTAGCCCGCTGCTTTTTTGGTATACTACTAGAGAAGAGAAAAA
AGCAAAAGGAGGCGGCTTAT

Downstream 100 bases:

>100_bases
ACAAAGAGGCTGGGGACAAAACGAGCCAAGAAATTCAATGAAAAGGAGTACGCTCATCGAACATCGATAAGCGTACTCTT
TTTTGCCTAGATTTTTCTGT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MKAIVSLNHLDFHGLAILAAAQKIHPDAIVVLPPIYQHAVKRFLDDYKTDFSFQHDGKLSWNEVDEIVYVDWEDEKQESL
YRSLPASAVETNLWRTIKATKRGVPITTLLYEMKRKQISVTAIEATLFALGLYSSTHHLTLPSTTASDGDACAYLLEKGA
DLRVVNDYLQQAPLAEKIASVMSKPVITVQTSQLIDEVWQTLLRSGHSGFPVVDETGALAGVITRMDLAKARQFGMGEAQ
VTEVMSMPNITLRANDSIDAACAHLAYNQVGRLPVVGDNNEPIGIVTRTDIVRSLYPNKHAVAPSELASYFGKQTCSFLQ
KIGAFADELQVPVYLVGGLVRDFFLKRPHKDIDLVVEGDGIAFAKQLATAFGGSVRSHESFGTATWANEQDIDIVTCRKE
FYLQKGALPTVRPASIYEDLARRDFSINAMAIQINRSSFGNVLDVFQGKQALIDKHIRILHPLSFIEDPTRLFRAVRFGL
RLNFSLSSETIHQATKTGAALHHISAKRLRQELDLLANEGVLFEGFRQLADLHVWTTLFGSRFSERAWRHMANLQQHGLN
DGMFFLLAGAVDCTRLDVASRYALTKQEKRLVEETSLPVWQQLAPTASLGEAHRDLARISSEIVRFYSEAELPLSPLLRR
YAEKRTQFKPLLTGADLLKAGYQPGPVFTQWLLEIECLQLDGHIATKDQALAWIAEKT

Sequences:

>Translated_698_residues
MKAIVSLNHLDFHGLAILAAAQKIHPDAIVVLPPIYQHAVKRFLDDYKTDFSFQHDGKLSWNEVDEIVYVDWEDEKQESL
YRSLPASAVETNLWRTIKATKRGVPITTLLYEMKRKQISVTAIEATLFALGLYSSTHHLTLPSTTASDGDACAYLLEKGA
DLRVVNDYLQQAPLAEKIASVMSKPVITVQTSQLIDEVWQTLLRSGHSGFPVVDETGALAGVITRMDLAKARQFGMGEAQ
VTEVMSMPNITLRANDSIDAACAHLAYNQVGRLPVVGDNNEPIGIVTRTDIVRSLYPNKHAVAPSELASYFGKQTCSFLQ
KIGAFADELQVPVYLVGGLVRDFFLKRPHKDIDLVVEGDGIAFAKQLATAFGGSVRSHESFGTATWANEQDIDIVTCRKE
FYLQKGALPTVRPASIYEDLARRDFSINAMAIQINRSSFGNVLDVFQGKQALIDKHIRILHPLSFIEDPTRLFRAVRFGL
RLNFSLSSETIHQATKTGAALHHISAKRLRQELDLLANEGVLFEGFRQLADLHVWTTLFGSRFSERAWRHMANLQQHGLN
DGMFFLLAGAVDCTRLDVASRYALTKQEKRLVEETSLPVWQQLAPTASLGEAHRDLARISSEIVRFYSEAELPLSPLLRR
YAEKRTQFKPLLTGADLLKAGYQPGPVFTQWLLEIECLQLDGHIATKDQALAWIAEKT
>Mature_698_residues
MKAIVSLNHLDFHGLAILAAAQKIHPDAIVVLPPIYQHAVKRFLDDYKTDFSFQHDGKLSWNEVDEIVYVDWEDEKQESL
YRSLPASAVETNLWRTIKATKRGVPITTLLYEMKRKQISVTAIEATLFALGLYSSTHHLTLPSTTASDGDACAYLLEKGA
DLRVVNDYLQQAPLAEKIASVMSKPVITVQTSQLIDEVWQTLLRSGHSGFPVVDETGALAGVITRMDLAKARQFGMGEAQ
VTEVMSMPNITLRANDSIDAACAHLAYNQVGRLPVVGDNNEPIGIVTRTDIVRSLYPNKHAVAPSELASYFGKQTCSFLQ
KIGAFADELQVPVYLVGGLVRDFFLKRPHKDIDLVVEGDGIAFAKQLATAFGGSVRSHESFGTATWANEQDIDIVTCRKE
FYLQKGALPTVRPASIYEDLARRDFSINAMAIQINRSSFGNVLDVFQGKQALIDKHIRILHPLSFIEDPTRLFRAVRFGL
RLNFSLSSETIHQATKTGAALHHISAKRLRQELDLLANEGVLFEGFRQLADLHVWTTLFGSRFSERAWRHMANLQQHGLN
DGMFFLLAGAVDCTRLDVASRYALTKQEKRLVEETSLPVWQQLAPTASLGEAHRDLARISSEIVRFYSEAELPLSPLLRR
YAEKRTQFKPLLTGADLLKAGYQPGPVFTQWLLEIECLQLDGHIATKDQALAWIAEKT

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=219, Percent_Identity=30.5936073059361, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 77879; Mature: 77879

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIVSLNHLDFHGLAILAAAQKIHPDAIVVLPPIYQHAVKRFLDDYKTDFSFQHDGKLS
CCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
WNEVDEIVYVDWEDEKQESLYRSLPASAVETNLWRTIKATKRGVPITTLLYEMKRKQISV
CCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEH
TAIEATLFALGLYSSTHHLTLPSTTASDGDACAYLLEKGADLRVVNDYLQQAPLAEKIAS
HHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHH
VMSKPVITVQTSQLIDEVWQTLLRSGHSGFPVVDETGALAGVITRMDLAKARQFGMGEAQ
HHHCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
VTEVMSMPNITLRANDSIDAACAHLAYNQVGRLPVVGDNNEPIGIVTRTDIVRSLYPNKH
HHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHCCCCC
AVAPSELASYFGKQTCSFLQKIGAFADELQVPVYLVGGLVRDFFLKRPHKDIDLVVEGDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCC
IAFAKQLATAFGGSVRSHESFGTATWANEQDIDIVTCRKEFYLQKGALPTVRPASIYEDL
HHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCEEEEECHHHHHHCCCCCCCCHHHHHHHH
ARRDFSINAMAIQINRSSFGNVLDVFQGKQALIDKHIRILHPLSFIEDPTRLFRAVRFGL
HHCCCCEEEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RLNFSLSSETIHQATKTGAALHHISAKRLRQELDLLANEGVLFEGFRQLADLHVWTTLFG
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SRFSERAWRHMANLQQHGLNDGMFFLLAGAVDCTRLDVASRYALTKQEKRLVEETSLPVW
HHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QQLAPTASLGEAHRDLARISSEIVRFYSEAELPLSPLLRRYAEKRTQFKPLLTGADLLKA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCHHHHHC
GYQPGPVFTQWLLEIECLQLDGHIATKDQALAWIAEKT
CCCCCHHHHHHHHHHHEEEECCCEECCCHHHHEEECCC
>Mature Secondary Structure
MKAIVSLNHLDFHGLAILAAAQKIHPDAIVVLPPIYQHAVKRFLDDYKTDFSFQHDGKLS
CCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
WNEVDEIVYVDWEDEKQESLYRSLPASAVETNLWRTIKATKRGVPITTLLYEMKRKQISV
CCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEH
TAIEATLFALGLYSSTHHLTLPSTTASDGDACAYLLEKGADLRVVNDYLQQAPLAEKIAS
HHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHH
VMSKPVITVQTSQLIDEVWQTLLRSGHSGFPVVDETGALAGVITRMDLAKARQFGMGEAQ
HHHCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
VTEVMSMPNITLRANDSIDAACAHLAYNQVGRLPVVGDNNEPIGIVTRTDIVRSLYPNKH
HHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHCCCCC
AVAPSELASYFGKQTCSFLQKIGAFADELQVPVYLVGGLVRDFFLKRPHKDIDLVVEGDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCC
IAFAKQLATAFGGSVRSHESFGTATWANEQDIDIVTCRKEFYLQKGALPTVRPASIYEDL
HHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCEEEEECHHHHHHCCCCCCCCHHHHHHHH
ARRDFSINAMAIQINRSSFGNVLDVFQGKQALIDKHIRILHPLSFIEDPTRLFRAVRFGL
HHCCCCEEEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RLNFSLSSETIHQATKTGAALHHISAKRLRQELDLLANEGVLFEGFRQLADLHVWTTLFG
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SRFSERAWRHMANLQQHGLNDGMFFLLAGAVDCTRLDVASRYALTKQEKRLVEETSLPVW
HHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QQLAPTASLGEAHRDLARISSEIVRFYSEAELPLSPLLRRYAEKRTQFKPLLTGADLLKA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCHHHHHC
GYQPGPVFTQWLLEIECLQLDGHIATKDQALAWIAEKT
CCCCCHHHHHHHHHHHEEEECCCEECCCHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]