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The map label for this gene is dat [H]

Identifier: 56964254

GI number: 56964254

Start: 2610877

End: 2611731

Strand: Direct

Name: dat [H]

Synonym: ABC2489

Alternate gene names: 56964254

Gene position: 2610877-2611731 (Clockwise)

Preceding gene: 56964250

Following gene: 56964257

Centisome position: 60.66

GC content: 45.61

Gene sequence:

>855_bases
ATGAACCATATTATCGTGAATGACTCCATCGTCCCTGAAACAGAGGCCCATGTTTCATACAATGACCGCGGGTACCATTT
CGGTGATGGCATTTATGAAGTTATACGCATTTATGAAGGAAGTTATTTTGCGCTCGATGCTCATTTAGACCGGTTGTATG
CCAGCGCAGAAAAACTCGACATGCGCATCCCTTATCAAAAAGAAACGTTGGCTGCCCGCCTAAACGATTACAAGAACACC
GAAAAAATTGAAAATGGCTCTGTTTACGTCCAATTTACAAGGGGCGCAGGCGCTCGCAACCATTTGTATACCCGTGAAGA
AACGCCTGTTTTAACTGGCTTTGCACTCCCTGATAAGCCGATGCAAGCAAGTCAGGAAAAAGGCGTTTCCGTTTATGTAA
CGCCTGATATCCGTTGGCTCCGCTGTGACATAAAAACCATCAATTTGCTAGGCAATGTCCTCGCCAAACGGAAAGCAAGC
GACCACGATTGTGCAGAGGCGGTGCTTTACCGTGATGAGGCGGTCACAGAAGGGTCTTCATCTAACTTATTTCTTGTTAA
CAATGAGACTTTATACACACACCCGGCAAACAATCTAATTTTAAATGGCATTACACGCCAAGAAATCATCTCGATTGCCA
AGAACATGGGGATCGATGTTGTGGAAGAGCCGTTTCCAAAAGAAGTGCTTGCTCATGCGGAAGAAGCGTTCATTACTAGC
ACATCGATTGAAATTACGCCTGTCCATACGTTTAAAGGCGATGTAGTGGCTACACTGTCCGTCGGCCCTGTAACAAGAAA
GCTGCAACAAGCACTTGCAGAGCATATTCACACCCAAACAAGCGCTTCCGTTTAG

Upstream 100 bases:

>100_bases
GTATCTCGGGTGAATTTCATTACTCCCATTGACAAACAAGCGTTCTTTTTGGAAAATAGAAGAGACGTCAAGAAGAAAAT
ATAGGAAACGAGGGCTAAAA

Downstream 100 bases:

>100_bases
GAGAGCGCTTGTTTGGGTTTGTCGACTATGTCGACAAACCGTCAGCTGATAGAAAACGCTTGTCAGACGAGGAATGCAGG
CGAGGGAAGATGTGAATAGA

Product: D-alanine aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNHIIVNDSIVPETEAHVSYNDRGYHFGDGIYEVIRIYEGSYFALDAHLDRLYASAEKLDMRIPYQKETLAARLNDYKNT
EKIENGSVYVQFTRGAGARNHLYTREETPVLTGFALPDKPMQASQEKGVSVYVTPDIRWLRCDIKTINLLGNVLAKRKAS
DHDCAEAVLYRDEAVTEGSSSNLFLVNNETLYTHPANNLILNGITRQEIISIAKNMGIDVVEEPFPKEVLAHAEEAFITS
TSIEITPVHTFKGDVVATLSVGPVTRKLQQALAEHIHTQTSASV

Sequences:

>Translated_284_residues
MNHIIVNDSIVPETEAHVSYNDRGYHFGDGIYEVIRIYEGSYFALDAHLDRLYASAEKLDMRIPYQKETLAARLNDYKNT
EKIENGSVYVQFTRGAGARNHLYTREETPVLTGFALPDKPMQASQEKGVSVYVTPDIRWLRCDIKTINLLGNVLAKRKAS
DHDCAEAVLYRDEAVTEGSSSNLFLVNNETLYTHPANNLILNGITRQEIISIAKNMGIDVVEEPFPKEVLAHAEEAFITS
TSIEITPVHTFKGDVVATLSVGPVTRKLQQALAEHIHTQTSASV
>Mature_284_residues
MNHIIVNDSIVPETEAHVSYNDRGYHFGDGIYEVIRIYEGSYFALDAHLDRLYASAEKLDMRIPYQKETLAARLNDYKNT
EKIENGSVYVQFTRGAGARNHLYTREETPVLTGFALPDKPMQASQEKGVSVYVTPDIRWLRCDIKTINLLGNVLAKRKAS
DHDCAEAVLYRDEAVTEGSSSNLFLVNNETLYTHPANNLILNGITRQEIISIAKNMGIDVVEEPFPKEVLAHAEEAFITS
TSIEITPVHTFKGDVVATLSVGPVTRKLQQALAEHIHTQTSASV

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=289, Percent_Identity=28.719723183391, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787338, Length=251, Percent_Identity=27.4900398406374, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 31706; Mature: 31706

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHIIVNDSIVPETEAHVSYNDRGYHFGDGIYEVIRIYEGSYFALDAHLDRLYASAEKLD
CCEEEECCCCCCCCCCEEEECCCCEECCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHE
MRIPYQKETLAARLNDYKNTEKIENGSVYVQFTRGAGARNHLYTREETPVLTGFALPDKP
EECCCHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCCCCEECCCCCEEEEECCCCCC
MQASQEKGVSVYVTPDIRWLRCDIKTINLLGNVLAKRKASDHDCAEAVLYRDEAVTEGSS
CCHHHHCCCEEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
SNLFLVNNETLYTHPANNLILNGITRQEIISIAKNMGIDVVEEPFPKEVLAHAEEAFITS
CCEEEEECCEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEE
TSIEITPVHTFKGDVVATLSVGPVTRKLQQALAEHIHTQTSASV
CCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNHIIVNDSIVPETEAHVSYNDRGYHFGDGIYEVIRIYEGSYFALDAHLDRLYASAEKLD
CCEEEECCCCCCCCCCEEEECCCCEECCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHE
MRIPYQKETLAARLNDYKNTEKIENGSVYVQFTRGAGARNHLYTREETPVLTGFALPDKP
EECCCHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCCCCEECCCCCEEEEECCCCCC
MQASQEKGVSVYVTPDIRWLRCDIKTINLLGNVLAKRKASDHDCAEAVLYRDEAVTEGSS
CCHHHHCCCEEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
SNLFLVNNETLYTHPANNLILNGITRQEIISIAKNMGIDVVEEPFPKEVLAHAEEAFITS
CCEEEEECCEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEE
TSIEITPVHTFKGDVVATLSVGPVTRKLQQALAEHIHTQTSASV
CCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA