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The map label for this gene is aroK [H]

Identifier: 56964246

GI number: 56964246

Start: 2604534

End: 2605079

Strand: Direct

Name: aroK [H]

Synonym: ABC2481

Alternate gene names: 56964246

Gene position: 2604534-2605079 (Clockwise)

Preceding gene: 56964220

Following gene: 56964250

Centisome position: 60.52

GC content: 42.67

Gene sequence:

>546_bases
ATGAGAACAGAAGTGCCTTTAATTGAAAAAAACATTGTTTTAGTCGGGTTTATGGGCGTCGGCAAAACAAGCATTGGCCA
AGCGCTCGCCAAAAAAATGTACCGTGATTTTATTGATATTGACGCCGAAATCGAAGCAAAGTTGAACATGAGCATTCCCA
CGATTTTTGAAAAACATGGGGAAGCTTTTTTTCGCCAACATGAAAAGGAAGCCGTTTTTTCTGCTTGCAATGAAAAATTG
AAAATTATTTCCCTTGGCGGCGGCGCTTTTACACAAGAAGCGATCCGCCAGCGCTGCCTTGCGAACTGCATTGTCATCCA
TTTGGATCTTTCATTTGAGTCTTGGAAAGATCGTGTCCACATGCTGATTGACAACCGCCCGATTCTCCAAGGAAAAACGA
TGCCTGAATTAAAACAACTATACACGGTAAGACGCAAGGCTTATGAAAATTATCACTCCCGCTTTTCGACAGATGCGTTC
AATGTCGATGAAGCCGCAGAGTATTTAAAAGAATCGCTCCACCTCGCCTATGAAATCGAAGGCTAA

Upstream 100 bases:

>100_bases
GCCCATCCTGCCGTTTCGTAAAAACGAGCATACGCCTCCCCACGCTGCTGATTTTTTTGCTATACTAAAAGCAAATTGAT
TGTAAAAGAGAGTGCCACCT

Downstream 100 bases:

>100_bases
AAAAGCGGCTTTTCCTTGGTAGTCAGGAAAAGCCGCTTTCATTCTGTTATGCTCTTGATACGTAAGAAGCCGATCTTGTG
TCAATTACAAGCTTATCGCC

Product: shikimate kinase

Products: NA

Alternate protein names: SK [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MRTEVPLIEKNIVLVGFMGVGKTSIGQALAKKMYRDFIDIDAEIEAKLNMSIPTIFEKHGEAFFRQHEKEAVFSACNEKL
KIISLGGGAFTQEAIRQRCLANCIVIHLDLSFESWKDRVHMLIDNRPILQGKTMPELKQLYTVRRKAYENYHSRFSTDAF
NVDEAAEYLKESLHLAYEIEG

Sequences:

>Translated_181_residues
MRTEVPLIEKNIVLVGFMGVGKTSIGQALAKKMYRDFIDIDAEIEAKLNMSIPTIFEKHGEAFFRQHEKEAVFSACNEKL
KIISLGGGAFTQEAIRQRCLANCIVIHLDLSFESWKDRVHMLIDNRPILQGKTMPELKQLYTVRRKAYENYHSRFSTDAF
NVDEAAEYLKESLHLAYEIEG
>Mature_181_residues
MRTEVPLIEKNIVLVGFMGVGKTSIGQALAKKMYRDFIDIDAEIEAKLNMSIPTIFEKHGEAFFRQHEKEAVFSACNEKL
KIISLGGGAFTQEAIRQRCLANCIVIHLDLSFESWKDRVHMLIDNRPILQGKTMPELKQLYTVRRKAYENYHSRFSTDAF
NVDEAAEYLKESLHLAYEIEG

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate [H]

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family [H]

Homologues:

Organism=Escherichia coli, GI87082255, Length=142, Percent_Identity=31.6901408450704, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786587, Length=154, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000623
- InterPro:   IPR023000 [H]

Pfam domain/function: PF01202 SKI [H]

EC number: =2.7.1.71 [H]

Molecular weight: Translated: 20773; Mature: 20773

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTEVPLIEKNIVLVGFMGVGKTSIGQALAKKMYRDFIDIDAEIEAKLNMSIPTIFEKHG
CCCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
EAFFRQHEKEAVFSACNEKLKIISLGGGAFTQEAIRQRCLANCIVIHLDLSFESWKDRVH
HHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHH
MLIDNRPILQGKTMPELKQLYTVRRKAYENYHSRFSTDAFNVDEAAEYLKESLHLAYEIE
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEEC
G
C
>Mature Secondary Structure
MRTEVPLIEKNIVLVGFMGVGKTSIGQALAKKMYRDFIDIDAEIEAKLNMSIPTIFEKHG
CCCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
EAFFRQHEKEAVFSACNEKLKIISLGGGAFTQEAIRQRCLANCIVIHLDLSFESWKDRVH
HHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHH
MLIDNRPILQGKTMPELKQLYTVRRKAYENYHSRFSTDAFNVDEAAEYLKESLHLAYEIE
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEEC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]