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The map label for this gene is ydgK [H]

Identifier: 56963678

GI number: 56963678

Start: 2028802

End: 2030022

Strand: Direct

Name: ydgK [H]

Synonym: ABC1913

Alternate gene names: 56963678

Gene position: 2028802-2030022 (Clockwise)

Preceding gene: 56963677

Following gene: 56963680

Centisome position: 47.14

GC content: 48.24

Gene sequence:

>1221_bases
ATGAAACAGAATCCAACAGGAATGGAGAGGTTGGCGCTTGCGTTTTTGCTTGGAATGCTAGCTGTCCTTGGGCCGCTCAA
TATTGATATGTATTTGCCGAGCTTTTTGCAAATTAGCGAAGATCTGCATGCGCCCGCTTCGCTTGTACAACTAAGTTTAA
CAACGTGCTTAATCGGTTTAGCGATCGGGCAACTTGTCGTCGGCCCGATCAGCGACGCAAAGGGAAGGAAACGGCCATTG
CTCATTTCAATTACGTTATTTGCCGCTGCTTCGCTTTTATGTGCGTTTGCTCCAAATATTGGCGTTTTGATTGCTGGCCG
CTTTTTACAAGGATTTACGGCAGCAGCTGGGATTGTCGTGTCTCGTGCGGTTGTCCGTGATGTTTTTACAGGCCAAGCAT
TAACAAAATTCTTTGCGTTGCTTATGGTGATCAATGCCGTGGCGCCGATGTTAGCACCAATGGCGGGCGGGGCGATTTTG
CTGTTGCCAGGGGCGCGCTGGGGGTGGATTTTCTTGTTTTTGTCTTTGCTCGGGGTCTTAATCGTTCTCGTTGCAGGAAT
AAAGCTAGAAGAATCGCTTCCACCAAGCAAGCGAGTGCCAAGCTCTTTGTCGCATATCCTATGGACATTCAAAGATTTGC
TTAAAGACCGTTCTTTTATCGGCTATGCACTTACACTCGGGCTCGCTCATGGCGGCAGTTTTGCCTATGTGGCGGGCACT
CCGTTTGTTTATCAAGGCATTTACGGTGTAAACGAACAAGTTTATAGTGTGTTATTTGGCATCAATGGTCTAGCGATCAT
CACCGGTACATTCGTGGTTGGCCGTTTAGCCGGCATAGTTCATGAACGAACGTTGTTGAAAACAGGGGTGATGGTTGCGA
GCTCAGCTACAGCTATTCTTGTCTGCATGGCGGCCATTCAAGGGCCATTATGGACAATTGTCGTTTCCATTTTTATTTAC
ATGACGTCAATGGGAATGATCACAACCTCTTCCTTTACGTTAGGAATTGCCAAACAAAGCCATCGTGCCGGCAGTGCTAG
CGCGATGCTCGGCATGTTGCCCTTATTCCTTGGAGCGATGGTTGCACCGCTTGTCGGCATCGATGAATCAACAGCAGTGC
CGATGGCAGCGATTATGTTTGCCACAAGCGTGTTGGCCCTTAGTACATGCCTTGGTTTGACGAAAAAAGAGAGCAGTCCT
AGCCAAGTCGAAAAACGGTAA

Upstream 100 bases:

>100_bases
AAATGTCAACAGACCATGCTCGTTTTTTGGGGCAAAGCCCTTTACAAACATGGTTTCCCTAAAGTAGACTGAGTTTGTTT
ATCAGAAATGGGGAGACAGA

Downstream 100 bases:

>100_bases
CGGTCCAAAGCAAAATAGCGCTAAATGAAGAAGGGGAATGGCCCTGGAAAAGAAAGAAAAGCAACAGAGAAGCGACAGGC
TCTGTTGCTTTTTTACTGCT

Product: bicyclomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKQNPTGMERLALAFLLGMLAVLGPLNIDMYLPSFLQISEDLHAPASLVQLSLTTCLIGLAIGQLVVGPISDAKGRKRPL
LISITLFAAASLLCAFAPNIGVLIAGRFLQGFTAAAGIVVSRAVVRDVFTGQALTKFFALLMVINAVAPMLAPMAGGAIL
LLPGARWGWIFLFLSLLGVLIVLVAGIKLEESLPPSKRVPSSLSHILWTFKDLLKDRSFIGYALTLGLAHGGSFAYVAGT
PFVYQGIYGVNEQVYSVLFGINGLAIITGTFVVGRLAGIVHERTLLKTGVMVASSATAILVCMAAIQGPLWTIVVSIFIY
MTSMGMITTSSFTLGIAKQSHRAGSASAMLGMLPLFLGAMVAPLVGIDESTAVPMAAIMFATSVLALSTCLGLTKKESSP
SQVEKR

Sequences:

>Translated_406_residues
MKQNPTGMERLALAFLLGMLAVLGPLNIDMYLPSFLQISEDLHAPASLVQLSLTTCLIGLAIGQLVVGPISDAKGRKRPL
LISITLFAAASLLCAFAPNIGVLIAGRFLQGFTAAAGIVVSRAVVRDVFTGQALTKFFALLMVINAVAPMLAPMAGGAIL
LLPGARWGWIFLFLSLLGVLIVLVAGIKLEESLPPSKRVPSSLSHILWTFKDLLKDRSFIGYALTLGLAHGGSFAYVAGT
PFVYQGIYGVNEQVYSVLFGINGLAIITGTFVVGRLAGIVHERTLLKTGVMVASSATAILVCMAAIQGPLWTIVVSIFIY
MTSMGMITTSSFTLGIAKQSHRAGSASAMLGMLPLFLGAMVAPLVGIDESTAVPMAAIMFATSVLALSTCLGLTKKESSP
SQVEKR
>Mature_406_residues
MKQNPTGMERLALAFLLGMLAVLGPLNIDMYLPSFLQISEDLHAPASLVQLSLTTCLIGLAIGQLVVGPISDAKGRKRPL
LISITLFAAASLLCAFAPNIGVLIAGRFLQGFTAAAGIVVSRAVVRDVFTGQALTKFFALLMVINAVAPMLAPMAGGAIL
LLPGARWGWIFLFLSLLGVLIVLVAGIKLEESLPPSKRVPSSLSHILWTFKDLLKDRSFIGYALTLGLAHGGSFAYVAGT
PFVYQGIYGVNEQVYSVLFGINGLAIITGTFVVGRLAGIVHERTLLKTGVMVASSATAILVCMAAIQGPLWTIVVSIFIY
MTSMGMITTSSFTLGIAKQSHRAGSASAMLGMLPLFLGAMVAPLVGIDESTAVPMAAIMFATSVLALSTCLGLTKKESSP
SQVEKR

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=366, Percent_Identity=30.8743169398907, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI48994889, Length=224, Percent_Identity=36.1607142857143, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790146, Length=270, Percent_Identity=28.5185185185185, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082312, Length=254, Percent_Identity=28.3464566929134, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787065, Length=344, Percent_Identity=27.3255813953488, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790794, Length=338, Percent_Identity=22.4852071005917, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321577, Length=151, Percent_Identity=28.476821192053, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325414, Length=151, Percent_Identity=28.476821192053, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319657, Length=171, Percent_Identity=26.9005847953216, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42673; Mature: 42673

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQNPTGMERLALAFLLGMLAVLGPLNIDMYLPSFLQISEDLHAPASLVQLSLTTCLIGL
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AIGQLVVGPISDAKGRKRPLLISITLFAAASLLCAFAPNIGVLIAGRFLQGFTAAAGIVV
HHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHH
SRAVVRDVFTGQALTKFFALLMVINAVAPMLAPMAGGAILLLPGARWGWIFLFLSLLGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
IVLVAGIKLEESLPPSKRVPSSLSHILWTFKDLLKDRSFIGYALTLGLAHGGSFAYVAGT
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECC
PFVYQGIYGVNEQVYSVLFGINGLAIITGTFVVGRLAGIVHERTLLKTGVMVASSATAIL
CHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCMAAIQGPLWTIVVSIFIYMTSMGMITTSSFTLGIAKQSHRAGSASAMLGMLPLFLGAM
HHHHHHCCHHHHHHHHHHHHHHHCCHHEECCEEEEEHHHHCCCCHHHHHHHHHHHHHHHH
VAPLVGIDESTAVPMAAIMFATSVLALSTCLGLTKKESSPSQVEKR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure
MKQNPTGMERLALAFLLGMLAVLGPLNIDMYLPSFLQISEDLHAPASLVQLSLTTCLIGL
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AIGQLVVGPISDAKGRKRPLLISITLFAAASLLCAFAPNIGVLIAGRFLQGFTAAAGIVV
HHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHH
SRAVVRDVFTGQALTKFFALLMVINAVAPMLAPMAGGAILLLPGARWGWIFLFLSLLGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
IVLVAGIKLEESLPPSKRVPSSLSHILWTFKDLLKDRSFIGYALTLGLAHGGSFAYVAGT
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECC
PFVYQGIYGVNEQVYSVLFGINGLAIITGTFVVGRLAGIVHERTLLKTGVMVASSATAIL
CHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCMAAIQGPLWTIVVSIFIYMTSMGMITTSSFTLGIAKQSHRAGSASAMLGMLPLFLGAM
HHHHHHCCHHHHHHHHHHHHHHHCCHHEECCEEEEEHHHHCCCCHHHHHHHHHHHHHHHH
VAPLVGIDESTAVPMAAIMFATSVLALSTCLGLTKKESSPSQVEKR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]