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The map label for this gene is ubiE

Identifier: 56963651

GI number: 56963651

Start: 2003757

End: 2004464

Strand: Direct

Name: ubiE

Synonym: ABC1886

Alternate gene names: 56963651

Gene position: 2003757-2004464 (Clockwise)

Preceding gene: 56963650

Following gene: 56963652

Centisome position: 46.56

GC content: 44.49

Gene sequence:

>708_bases
GTGGCCCAATCGAAAGAACAACGCGTTCATGACGTATTTCAATCGATTTATAAAAAATACGATGTGATGAATTCTGTTAT
CAGTTTACAGCAACATAAAAGCTGGCGTAAAGATACAATGAAGCAAATGGATGTCCAAGCAGGAACGAGTGCTCTCGATG
TATGTTGTGGCACAGGCGATTGGACGTTGGCGCTGTCGGAAGCCGTTGGCGAGCAGGGGTCCGTGATTGGCCTTGATTTT
AGCGAAAATATGCTCGCGGTCGGACGTGAAAAAGTTGCAGCTGCTAAGCGCACTAATATCTCACTCGTCCACGGAAACGC
AATGGCGCTTCCGTATGATGACAACACGTTTGATTATGTGACGATCGGTTTTGGGTTGCGGAATGTTCCTGATTACATGC
AAGTGTTACAGGAGATGTGCCGAGTAGCGAAGCCTGGAGGCAAAATTGTTTGTCTCGAAACGTCGCAGCCAACCATACCA
GTCTTTAAACAACTATACTTTTTTTATTTTAAGCGGATTATGCCCCTTGCAGGAAAAGTGTTTGCAAAAAAATACGAAGA
ATATTCGTGGCTCCAAGAATCAACATTAGCGTTTCCAGGAAAAGACAAACTTCGGGACATGTTCGTTGAAGCTGGAATGA
AGGATGTTACAGTAAAGAGCTACACAGGCGGTGTTTGCGCTATGCATATGGGGATAAAACCGATATAG

Upstream 100 bases:

>100_bases
AGAGGCAATTAGAACAGCTACAAGAAGCAGTGGACAAGCAGAATAATAAAGCAAGTGCGTTTATAAAAACATTCCTTGAA
AAAGCAAGGAGGTAGCGGGA

Downstream 100 bases:

>100_bases
AAATGTGTGATATTGGAGAGGAGTAAGGCAGTAATGGCTACTGCATCTTTAAATAAAACGAAAATCTTTTTGCGAATGAT
TAAATTTGAACATACAATTT

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MAQSKEQRVHDVFQSIYKKYDVMNSVISLQQHKSWRKDTMKQMDVQAGTSALDVCCGTGDWTLALSEAVGEQGSVIGLDF
SENMLAVGREKVAAAKRTNISLVHGNAMALPYDDNTFDYVTIGFGLRNVPDYMQVLQEMCRVAKPGGKIVCLETSQPTIP
VFKQLYFFYFKRIMPLAGKVFAKKYEEYSWLQESTLAFPGKDKLRDMFVEAGMKDVTVKSYTGGVCAMHMGIKPI

Sequences:

>Translated_235_residues
MAQSKEQRVHDVFQSIYKKYDVMNSVISLQQHKSWRKDTMKQMDVQAGTSALDVCCGTGDWTLALSEAVGEQGSVIGLDF
SENMLAVGREKVAAAKRTNISLVHGNAMALPYDDNTFDYVTIGFGLRNVPDYMQVLQEMCRVAKPGGKIVCLETSQPTIP
VFKQLYFFYFKRIMPLAGKVFAKKYEEYSWLQESTLAFPGKDKLRDMFVEAGMKDVTVKSYTGGVCAMHMGIKPI
>Mature_234_residues
AQSKEQRVHDVFQSIYKKYDVMNSVISLQQHKSWRKDTMKQMDVQAGTSALDVCCGTGDWTLALSEAVGEQGSVIGLDFS
ENMLAVGREKVAAAKRTNISLVHGNAMALPYDDNTFDYVTIGFGLRNVPDYMQVLQEMCRVAKPGGKIVCLETSQPTIPV
FKQLYFFYFKRIMPLAGKVFAKKYEEYSWLQESTLAFPGKDKLRDMFVEAGMKDVTVKSYTGGVCAMHMGIKPI

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=265, Percent_Identity=36.6037735849057, Blast_Score=168, Evalue=3e-42,
Organism=Escherichia coli, GI48994983, Length=234, Percent_Identity=41.4529914529915, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17557039, Length=233, Percent_Identity=33.0472103004292, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323526, Length=249, Percent_Identity=30.5220883534137, Blast_Score=119, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24641702, Length=243, Percent_Identity=32.9218106995885, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_BACSK (Q5WGT4)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175382.1
- ProteinModelPortal:   Q5WGT4
- SMR:   Q5WGT4
- STRING:   Q5WGT4
- EnsemblBacteria:   EBBACT00000048552
- GeneID:   3204435
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1886
- NMPDR:   fig|66692.3.peg.1930
- eggNOG:   COG2226
- GeneTree:   EBGT00050000000522
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- BioCyc:   BCLA66692:ABC1886-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR014122
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR02752
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26360; Mature: 26229

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSKEQRVHDVFQSIYKKYDVMNSVISLQQHKSWRKDTMKQMDVQAGTSALDVCCGTGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCC
WTLALSEAVGEQGSVIGLDFSENMLAVGREKVAAAKRTNISLVHGNAMALPYDDNTFDYV
HHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCEEEECCCCCCCEEE
TIGFGLRNVPDYMQVLQEMCRVAKPGGKIVCLETSQPTIPVFKQLYFFYFKRIMPLAGKV
EECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
FAKKYEEYSWLQESTLAFPGKDKLRDMFVEAGMKDVTVKSYTGGVCAMHMGIKPI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCC
>Mature Secondary Structure 
AQSKEQRVHDVFQSIYKKYDVMNSVISLQQHKSWRKDTMKQMDVQAGTSALDVCCGTGD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCC
WTLALSEAVGEQGSVIGLDFSENMLAVGREKVAAAKRTNISLVHGNAMALPYDDNTFDYV
HHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCEEEECCCCCCCEEE
TIGFGLRNVPDYMQVLQEMCRVAKPGGKIVCLETSQPTIPVFKQLYFFYFKRIMPLAGKV
EECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
FAKKYEEYSWLQESTLAFPGKDKLRDMFVEAGMKDVTVKSYTGGVCAMHMGIKPI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA