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The map label for this gene is sleB [H]

Identifier: 56963635

GI number: 56963635

Start: 1989953

End: 1990843

Strand: Direct

Name: sleB [H]

Synonym: ABC1870

Alternate gene names: 56963635

Gene position: 1989953-1990843 (Clockwise)

Preceding gene: 56963634

Following gene: 56963636

Centisome position: 46.24

GC content: 45.68

Gene sequence:

>891_bases
ATGTTTCGAACAAAGCGAATCGCTCGGTGCCTTCTCGGTGCAACTTTAGCGTTTGCGGTTCTTTTTTTTAACAACGAAAT
GGTTGATGCCTTTAGTGACCAAGTCATTCAAAAAGGGGCAACAGGCGACGATGTCGTCGAATTGCAATCTCGTTTGCAGT
ATGTAGGGTATTATAATGGCAAAATTGATGGTGTTTTCGGTTGGGGGACGTACTGGGCTGTGCGGCATTATCAGTACGAA
TATGGGCTTGAAATTGATGGATTGGTCGGAGACGACATGAAAGCAAAGCTGGCAAGCACAACCGACTACGATAAAGCCTT
TGTGACAAAAGCACTGAACGAGGGCAGAAAGTTTTCTCACTATGGCGGTACACCAAAAGACATCCAGAAAGGGCCAAAAG
GTTCTGCAGATAAGCAAAAACAAAAAAAGGAAGCAGCAAAAGGTGGCGAGGCACAGCAGCCAGCTCCGAATCAGCCTGAA
ACTGGAAATGAACAAGAACCGATTATTGAAAAAGCAAACAATGTGCCTTCAGGTTATTCATCGAATGATATTCAACTGAT
GGCTCAGGCAGTGTATGGCGAGGCCCGTGGTGAGCCTTATGAAGGACAAGTCGCGGTGGCAGCAGTGATTGTCAACCGTT
TAAATTCAGCAACTTTCCCAGATACAGTCGCTGGCGTCATATATGAAGACCGCGCTTTTACGGCGGTAGCTGATGGGCAA
ATCAACTTAGGCGCAGATGAAACGTCAAGGCAAGCTGTGCTTGATGCGTTAAATGGGCAAGACCCGACCAACAATGCGCT
TTATTATTTCAATCCAGATACAGCGACATCAGATTGGATTTGGTCGCGGCCACAAATTAAACGGATTGGCAAACATATAT
TCTGCTATTAA

Upstream 100 bases:

>100_bases
TGCGTCTGTTTTTTTTGCATAAAAACACCCCAGCAAAACAAAGACTAGCAGTAACGACCAATTTCCCCACAAAAAAGTGG
GCATAAGGAGGAACGAAAAT

Downstream 100 bases:

>100_bases
AACGGAGGTGCTATTTGCATGTTTCGCAATCTTTTAATCGCTGCTTTAGCCGTTGCAGTAGTTGGCACAGGCGTTTGGGG
CTACCAGCAAAAGCAAGAAA

Product: spore cortex-lytic enzyme

Products: NA

Alternate protein names: SCLE [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MFRTKRIARCLLGATLAFAVLFFNNEMVDAFSDQVIQKGATGDDVVELQSRLQYVGYYNGKIDGVFGWGTYWAVRHYQYE
YGLEIDGLVGDDMKAKLASTTDYDKAFVTKALNEGRKFSHYGGTPKDIQKGPKGSADKQKQKKEAAKGGEAQQPAPNQPE
TGNEQEPIIEKANNVPSGYSSNDIQLMAQAVYGEARGEPYEGQVAVAAVIVNRLNSATFPDTVAGVIYEDRAFTAVADGQ
INLGADETSRQAVLDALNGQDPTNNALYYFNPDTATSDWIWSRPQIKRIGKHIFCY

Sequences:

>Translated_296_residues
MFRTKRIARCLLGATLAFAVLFFNNEMVDAFSDQVIQKGATGDDVVELQSRLQYVGYYNGKIDGVFGWGTYWAVRHYQYE
YGLEIDGLVGDDMKAKLASTTDYDKAFVTKALNEGRKFSHYGGTPKDIQKGPKGSADKQKQKKEAAKGGEAQQPAPNQPE
TGNEQEPIIEKANNVPSGYSSNDIQLMAQAVYGEARGEPYEGQVAVAAVIVNRLNSATFPDTVAGVIYEDRAFTAVADGQ
INLGADETSRQAVLDALNGQDPTNNALYYFNPDTATSDWIWSRPQIKRIGKHIFCY
>Mature_296_residues
MFRTKRIARCLLGATLAFAVLFFNNEMVDAFSDQVIQKGATGDDVVELQSRLQYVGYYNGKIDGVFGWGTYWAVRHYQYE
YGLEIDGLVGDDMKAKLASTTDYDKAFVTKALNEGRKFSHYGGTPKDIQKGPKGSADKQKQKKEAAKGGEAQQPAPNQPE
TGNEQEPIIEKANNVPSGYSSNDIQLMAQAVYGEARGEPYEGQVAVAAVIVNRLNSATFPDTVAGVIYEDRAFTAVADGQ
INLGADETSRQAVLDALNGQDPTNNALYYFNPDTATSDWIWSRPQIKRIGKHIFCY

Specific function: Required for spore cortex hydrolysis during germination [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: NA

Molecular weight: Translated: 32597; Mature: 32597

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRTKRIARCLLGATLAFAVLFFNNEMVDAFSDQVIQKGATGDDVVELQSRLQYVGYYNG
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECC
KIDGVFGWGTYWAVRHYQYEYGLEIDGLVGDDMKAKLASTTDYDKAFVTKALNEGRKFSH
EECEEECCHHEEHHEEEEEECCCEECCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCC
YGGTPKDIQKGPKGSADKQKQKKEAAKGGEAQQPAPNQPETGNEQEPIIEKANNVPSGYS
CCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
SNDIQLMAQAVYGEARGEPYEGQVAVAAVIVNRLNSATFPDTVAGVIYEDRAFTAVADGQ
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHEECCCEEEEEECCE
INLGADETSRQAVLDALNGQDPTNNALYYFNPDTATSDWIWSRPQIKRIGKHIFCY
EECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MFRTKRIARCLLGATLAFAVLFFNNEMVDAFSDQVIQKGATGDDVVELQSRLQYVGYYNG
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECC
KIDGVFGWGTYWAVRHYQYEYGLEIDGLVGDDMKAKLASTTDYDKAFVTKALNEGRKFSH
EECEEECCHHEEHHEEEEEECCCEECCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCC
YGGTPKDIQKGPKGSADKQKQKKEAAKGGEAQQPAPNQPETGNEQEPIIEKANNVPSGYS
CCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
SNDIQLMAQAVYGEARGEPYEGQVAVAAVIVNRLNSATFPDTVAGVIYEDRAFTAVADGQ
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHEECCCEEEEEECCE
INLGADETSRQAVLDALNGQDPTNNALYYFNPDTATSDWIWSRPQIKRIGKHIFCY
EECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132; 10484179 [H]