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The map label for this gene is gudB [H]

Identifier: 56963627

GI number: 56963627

Start: 1978780

End: 1980045

Strand: Direct

Name: gudB [H]

Synonym: ABC1862

Alternate gene names: 56963627

Gene position: 1978780-1980045 (Clockwise)

Preceding gene: 56963626

Following gene: 56963628

Centisome position: 45.98

GC content: 46.84

Gene sequence:

>1266_bases
GTGGAAAAGATGGACGAAAACCAAGAGGATAAATTAGATGTTCTTGCAGCGACTCAGTCCATTATACATAAGGCGCTAAA
AAAACTAGGCTATCCAGATGAAATGTATGAACTACTTAAAGAGCCTGTACGGATGTTGACCGTCCGTATTCCTGTAAGAA
TGGATGATGGATCAACGAAAATTTTTACAGGCTACCGTGCCCAACATAATGACGCTGTTGGTCCCACAAAAGGAGGCGTA
CGGTTTCATCCAGATGTTAGTGAAAAAGAAGTCAAGGCGCTGTCGATTTGGATGAGTTTAAAAGCAGGCATTGTCAACTT
GCCATACGGTGGAGGAAAAGGAGGCATTGTTTGCGACCCAAGGCAGATGTCGTTTAGAGAAGTGGAACGTCTAAGTCGTG
GCTATGTCCGCGCGATTAGCCAAATTGTTGGCCCGACAAAAGACATTCCTGCGCCAGATGTTTTTACCAATTCGCAAATC
ATGGCGTGGATGCTTGATGAATATAGCCGAATTCGCGAATTTGACTCACCCAATTTTATTACGGGAAAGCCGCTTGTGCT
CGGGGGGTCACATGGCCGAGAGGCAGCGACTGCAAAAGGTGTGACGATTTGCATCATGGAAGCGGCAAAAAAGAAAGGAA
TTGACCTTGAAGGGGCGCGCGTCATCATTCAAGGGTTTGGCAATGCAGGGAGCTTTTTGGCGAAGTTTATGGCCGACGCA
GGGGCATTAGTAGTGGGAATTGCCGATGCATATGGCGCTCTTTATGCAGAAGAAGGTTTAGATATTGACTATTTGCTTGA
TCGCCGCGATAGTTTCGGGACAGTAACAAATTTATTTAAAGATACGATTACAAATGAGGAGCTATTAGAAAAAGATTGTG
ATATTTTGGTTCCGGCAGCAATTGAAAACCAAATCACCTCACACAATGCAGGCAAGTTAAAAGCAGCGATTGTCGTCGAA
GCAGCTAATGGCCCGACTACGCTTGAGGCCACGCAAATCCTTGCAGAACGGGGCATTTTGCTTGTGCCTGATGTCTTGGC
AAGTGCTGGGGGCGTAACGGTCTCTTACTTTGAATGGGTGCAAAACAACCAGGGCTACTACTGGACGGATGAAGAAGTCG
AAGGACGGTTGCGGACAGTGCTGACGGAAGCGTTTAACAACATATACAACCTTGCTTTACGTCGTAAAGTCGACATGCGT
CTCGCTGCCTATATGGTCGGTGTGAGACGCATGGCCGAAGCTTCTCGGTTCCGTGGATGGGTTTGA

Upstream 100 bases:

>100_bases
GGAACTTGTTTCAGAAGACTAATTGGTTTTTAGAAAAAAGGATGGCAATTATGTGAGCATTTCATTAAGATAAAGATGTG
AGCAGCTGACTTCCAAAGAG

Downstream 100 bases:

>100_bases
TCTGCTATACTATGATGAGAAAATAAGTGCCCTTGTCCAAAGTTGCCTTTGAGGACAAGGGTTTTGAATGTTTTGATGAG
GTGAGGAATGTGGTAGATGC

Product: NAD-specific glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MEKMDENQEDKLDVLAATQSIIHKALKKLGYPDEMYELLKEPVRMLTVRIPVRMDDGSTKIFTGYRAQHNDAVGPTKGGV
RFHPDVSEKEVKALSIWMSLKAGIVNLPYGGGKGGIVCDPRQMSFREVERLSRGYVRAISQIVGPTKDIPAPDVFTNSQI
MAWMLDEYSRIREFDSPNFITGKPLVLGGSHGREAATAKGVTICIMEAAKKKGIDLEGARVIIQGFGNAGSFLAKFMADA
GALVVGIADAYGALYAEEGLDIDYLLDRRDSFGTVTNLFKDTITNEELLEKDCDILVPAAIENQITSHNAGKLKAAIVVE
AANGPTTLEATQILAERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTDEEVEGRLRTVLTEAFNNIYNLALRRKVDMR
LAAYMVGVRRMAEASRFRGWV

Sequences:

>Translated_421_residues
MEKMDENQEDKLDVLAATQSIIHKALKKLGYPDEMYELLKEPVRMLTVRIPVRMDDGSTKIFTGYRAQHNDAVGPTKGGV
RFHPDVSEKEVKALSIWMSLKAGIVNLPYGGGKGGIVCDPRQMSFREVERLSRGYVRAISQIVGPTKDIPAPDVFTNSQI
MAWMLDEYSRIREFDSPNFITGKPLVLGGSHGREAATAKGVTICIMEAAKKKGIDLEGARVIIQGFGNAGSFLAKFMADA
GALVVGIADAYGALYAEEGLDIDYLLDRRDSFGTVTNLFKDTITNEELLEKDCDILVPAAIENQITSHNAGKLKAAIVVE
AANGPTTLEATQILAERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTDEEVEGRLRTVLTEAFNNIYNLALRRKVDMR
LAAYMVGVRRMAEASRFRGWV
>Mature_421_residues
MEKMDENQEDKLDVLAATQSIIHKALKKLGYPDEMYELLKEPVRMLTVRIPVRMDDGSTKIFTGYRAQHNDAVGPTKGGV
RFHPDVSEKEVKALSIWMSLKAGIVNLPYGGGKGGIVCDPRQMSFREVERLSRGYVRAISQIVGPTKDIPAPDVFTNSQI
MAWMLDEYSRIREFDSPNFITGKPLVLGGSHGREAATAKGVTICIMEAAKKKGIDLEGARVIIQGFGNAGSFLAKFMADA
GALVVGIADAYGALYAEEGLDIDYLLDRRDSFGTVTNLFKDTITNEELLEKDCDILVPAAIENQITSHNAGKLKAAIVVE
AANGPTTLEATQILAERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTDEEVEGRLRTVLTEAFNNIYNLALRRKVDMR
LAAYMVGVRRMAEASRFRGWV

Specific function: The wild-type version has little or no activity [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=338, Percent_Identity=39.3491124260355, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI4885281, Length=338, Percent_Identity=38.4615384615385, Blast_Score=249, Evalue=5e-66,
Organism=Escherichia coli, GI1788059, Length=363, Percent_Identity=31.404958677686, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17544676, Length=339, Percent_Identity=41.0029498525074, Blast_Score=251, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6319256, Length=399, Percent_Identity=28.8220551378446, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324951, Length=395, Percent_Identity=28.1012658227848, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553475, Length=340, Percent_Identity=40, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI45549226, Length=340, Percent_Identity=40, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24649283, Length=328, Percent_Identity=40.2439024390244, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=38.3512544802867, Blast_Score=201, Evalue=8e-52,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 46379; Mature: 46379

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKMDENQEDKLDVLAATQSIIHKALKKLGYPDEMYELLKEPVRMLTVRIPVRMDDGSTK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEEEECCCCEE
IFTGYRAQHNDAVGPTKGGVRFHPDVSEKEVKALSIWMSLKAGIVNLPYGGGKGGIVCDP
EEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCEEECC
RQMSFREVERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMLDEYSRIREFDSPNFI
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
TGKPLVLGGSHGREAATAKGVTICIMEAAKKKGIDLEGARVIIQGFGNAGSFLAKFMADA
CCCCEEEECCCCCCCHHHCCEEEEEEEHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCC
GALVVGIADAYGALYAEEGLDIDYLLDRRDSFGTVTNLFKDTITNEELLEKDCDILVPAA
CEEEEEEHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHCCCCEEECHH
IENQITSHNAGKLKAAIVVEAANGPTTLEATQILAERGILLVPDVLASAGGVTVSYFEWV
HHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCEEECHHHHCCCCCEEHHHHHH
QNNQGYYWTDEEVEGRLRTVLTEAFNNIYNLALRRKVDMRLAAYMVGVRRMAEASRFRGW
HCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
V
C
>Mature Secondary Structure
MEKMDENQEDKLDVLAATQSIIHKALKKLGYPDEMYELLKEPVRMLTVRIPVRMDDGSTK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEEEECCCCEE
IFTGYRAQHNDAVGPTKGGVRFHPDVSEKEVKALSIWMSLKAGIVNLPYGGGKGGIVCDP
EEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCEEECC
RQMSFREVERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMLDEYSRIREFDSPNFI
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
TGKPLVLGGSHGREAATAKGVTICIMEAAKKKGIDLEGARVIIQGFGNAGSFLAKFMADA
CCCCEEEECCCCCCCHHHCCEEEEEEEHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCC
GALVVGIADAYGALYAEEGLDIDYLLDRRDSFGTVTNLFKDTITNEELLEKDCDILVPAA
CEEEEEEHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHCCCCEEECHH
IENQITSHNAGKLKAAIVVEAANGPTTLEATQILAERGILLVPDVLASAGGVTVSYFEWV
HHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCEEECHHHHCCCCCEEHHHHHH
QNNQGYYWTDEEVEGRLRTVLTEAFNNIYNLALRRKVDMRLAAYMVGVRRMAEASRFRGW
HCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829940 [H]