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The map label for this gene is rluB [H]

Identifier: 56963596

GI number: 56963596

Start: 1947734

End: 1948462

Strand: Direct

Name: rluB [H]

Synonym: ABC1831

Alternate gene names: 56963596

Gene position: 1947734-1948462 (Clockwise)

Preceding gene: 56963595

Following gene: 56963597

Centisome position: 45.26

GC content: 44.03

Gene sequence:

>729_bases
ATGGAACGATTGCAAAAAGTTATCGCCAAAGCAGGAGTGGCTTCGAGAAGAAAAGCAGAAGAATATATTGTTGAAGGTAG
AGTGAAAGTAAACGGCGAAACGGTTACAGAACTAGGGACAAAAGTAAATCCAAGCAAAGACGCTGTTGAAGTGGACGGCG
TTCCCCTTGATAAAGAAGAGCCTGTCTACCATTTGTTGTATAAACCGACTGGCGTCGTGTCAACATCAAATGACGAGAAA
GGGCGGAAAGTCGTCACAGATTTTATTGAAAGCGAACAAAGAATTTATCCGATTGGCCGGTTGGATTATGACACATCTGG
TTTGCTTCTGCTTACAAATGATGGAGAGTTTGCCCATCTGCTCATGCATCCCCGTTATAAAATTGAAAAAGAGTACATTG
CCAAAGTGAAAGGAATCCCTGAACGAAAACGGATCAAAGAACTTGAAACCGGGATTGTCTTAGAAGATGGCAAGACTGCC
CCGGCAAAAGCAAAAGTCTTGTCGATTGACAAGAAAAAGCAAACAGCAATTGTGCGGCTCATTATCCATGAAGGCAAAAA
CCGCCAAGTCCGCCGGATGTTTGAAGCAATTGGCCATCCTGTTGTAAAGTTAAAGCGCGAGCGCTACGGGTTTTTAACGC
TTGGCCATTTACATCCAGGCGAGTCACGGCCGTTAAAGCCGATTGAAGTCAAACAATTAAAGGAACTGGCTGTCACGAAA
CCGTCATAA

Upstream 100 bases:

>100_bases
TTATGTCATTTGTTTGATTTTTCGAGTCTGATTAAATCTCTACTTTCGCCTACTTGTATCAGCTGCTTGAAGGCGGTATG
ATGGCAAAGAGGTGAACACG

Downstream 100 bases:

>100_bases
AATTGCAGGCGGACAAAACGAAGAAAGCGCTATAATGTAAGAAAACGTTCACATTTTCGCGAGAGGAGGGGACGTCGCTT
GGGTAAAAGCAAAAAGAAAA

Product: ribosomal large subunit pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MERLQKVIAKAGVASRRKAEEYIVEGRVKVNGETVTELGTKVNPSKDAVEVDGVPLDKEEPVYHLLYKPTGVVSTSNDEK
GRKVVTDFIESEQRIYPIGRLDYDTSGLLLLTNDGEFAHLLMHPRYKIEKEYIAKVKGIPERKRIKELETGIVLEDGKTA
PAKAKVLSIDKKKQTAIVRLIIHEGKNRQVRRMFEAIGHPVVKLKRERYGFLTLGHLHPGESRPLKPIEVKQLKELAVTK
PS

Sequences:

>Translated_242_residues
MERLQKVIAKAGVASRRKAEEYIVEGRVKVNGETVTELGTKVNPSKDAVEVDGVPLDKEEPVYHLLYKPTGVVSTSNDEK
GRKVVTDFIESEQRIYPIGRLDYDTSGLLLLTNDGEFAHLLMHPRYKIEKEYIAKVKGIPERKRIKELETGIVLEDGKTA
PAKAKVLSIDKKKQTAIVRLIIHEGKNRQVRRMFEAIGHPVVKLKRERYGFLTLGHLHPGESRPLKPIEVKQLKELAVTK
PS
>Mature_242_residues
MERLQKVIAKAGVASRRKAEEYIVEGRVKVNGETVTELGTKVNPSKDAVEVDGVPLDKEEPVYHLLYKPTGVVSTSNDEK
GRKVVTDFIESEQRIYPIGRLDYDTSGLLLLTNDGEFAHLLMHPRYKIEKEYIAKVKGIPERKRIKELETGIVLEDGKTA
PAKAKVLSIDKKKQTAIVRLIIHEGKNRQVRRMFEAIGHPVVKLKRERYGFLTLGHLHPGESRPLKPIEVKQLKELAVTK
PS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=249, Percent_Identity=37.7510040160643, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=37.5527426160338, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=35.0210970464135, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87081838, Length=172, Percent_Identity=37.2093023255814, Blast_Score=107, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 27332; Mature: 27332

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKVIAKAGVASRRKAEEYIVEGRVKVNGETVTELGTKVNPSKDAVEVDGVPLDKEE
CHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCHHHHCCCCCCCCCCEEECCCCCCCCC
PVYHLLYKPTGVVSTSNDEKGRKVVTDFIESEQRIYPIGRLDYDTSGLLLLTNDGEFAHL
CEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCEEEE
LMHPRYKIEKEYIAKVKGIPERKRIKELETGIVLEDGKTAPAKAKVLSIDKKKQTAIVRL
EECCCHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCCCCEEEEECCCHHHHEEEEE
IIHEGKNRQVRRMFEAIGHPVVKLKRERYGFLTLGHLHPGESRPLKPIEVKQLKELAVTK
EEECCCCHHHHHHHHHHCCHHEEEHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCC
PS
CC
>Mature Secondary Structure
MERLQKVIAKAGVASRRKAEEYIVEGRVKVNGETVTELGTKVNPSKDAVEVDGVPLDKEE
CHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCHHHHCCCCCCCCCCEEECCCCCCCCC
PVYHLLYKPTGVVSTSNDEKGRKVVTDFIESEQRIYPIGRLDYDTSGLLLLTNDGEFAHL
CEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCEEEE
LMHPRYKIEKEYIAKVKGIPERKRIKELETGIVLEDGKTAPAKAKVLSIDKKKQTAIVRL
EECCCHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCCCCEEEEECCCHHHHEEEEE
IIHEGKNRQVRRMFEAIGHPVVKLKRERYGFLTLGHLHPGESRPLKPIEVKQLKELAVTK
EEECCCCHHHHHHHHHHCCHHEEEHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCC
PS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]