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The map label for this gene is kinA [H]

Identifier: 56963514

GI number: 56963514

Start: 1872251

End: 1873216

Strand: Direct

Name: kinA [H]

Synonym: ABC1749

Alternate gene names: 56963514

Gene position: 1872251-1873216 (Clockwise)

Preceding gene: 56963510

Following gene: 56963515

Centisome position: 43.5

GC content: 43.17

Gene sequence:

>966_bases
ATGGAGATAGAGGAGCTGTTCGGTTTGTTGGATTTATCAGAAGATTGCTACTTCGTTCACGATAGGGCAACCATTTTATT
TGCGAATCGAGCTGCCCATGAGTGTTTAGGAAAACCGATCGGCTATTTGCACGGGAAACGACTTAAACAGCTTATTTCCG
AAGAAGACATCGTATGTGCCGGGAAACCATTTCAGTTGGGCAAAAAACGTCTTTATGCGTCCGTATTAAAGCAACCTTTT
AAACAAGCGCACAAACGAAAAGAAGCAATGGAAGTTGTCTCTCGCTTATCGGCGGGGGTGGCTCACGAACTCCGCAATCC
ACTGACAACGATAAAAGGATTTATCCAACTCTATTCGGAAGTTGGCGAGTTGAGGGAAAGCCAGATCAACATCATGAATA
TAGAGTTGGAGCGAATTGAAAACATTATTAATGACTATGTGCTGTTAGCTGAAGAACCTCCTGCCCGTGAAAACGAATCG
ATTCAAATGGCAGCCTTTTTGCAGTCGCTCTTAAAAAAGAACAAAGTGAAGCGGCTTACCGACGGTCACCCTATTAAGCT
AAACATCAGCGAGGAAGCAACCGTATTGGGGTCAAGAAAAGAGCTTTTTCTATTGTTTTACAATTTGCTTGAAAATGCGA
TTGATGCTAGTGTGAAACATTCGCCTGTGCATATTACTATGTATGCGGAAGGCCCGAATGTTTGTGTGGAAATAACAGAC
TTTGGTTCTGGAATGCCCGCGGAGAGACTCAATTATATAGGCCAGCCCTTTTATTCGACAAAAGAAAAAGGAACAGGCCT
TGGCTTAATGATTTGTTACCGCGTCGTTGAAACGCATGGCGGCTTGATTGATATTGAGTCGAAAGATAAAAGGGGAACGA
ATGTGAAAGTCATCTTGCCAATGACAACGAAAAACAAGGCTGGCTCAATGGTACGCGAAAAAGCGGCGATGTTAACCTAT
GGATAA

Upstream 100 bases:

>100_bases
GAAAGTGGGTTTTAAAAATGAAATCAATGAATGAAAATGGTTTTATCTCAATTTTAGTAGGAGCTGGCGCTTGTCAACAA
GGGAATCAAGGAAGATCGCA

Downstream 100 bases:

>100_bases
AGCGTGCTCAATCGTTAACCATTATTGCAAGCATAAGAACATCGTTGTCGTGTTAACGCAAAACAAAGGAAAAGGAGAAT
AGATCCCGCAAGTTGGGCAT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MEIEELFGLLDLSEDCYFVHDRATILFANRAAHECLGKPIGYLHGKRLKQLISEEDIVCAGKPFQLGKKRLYASVLKQPF
KQAHKRKEAMEVVSRLSAGVAHELRNPLTTIKGFIQLYSEVGELRESQINIMNIELERIENIINDYVLLAEEPPARENES
IQMAAFLQSLLKKNKVKRLTDGHPIKLNISEEATVLGSRKELFLLFYNLLENAIDASVKHSPVHITMYAEGPNVCVEITD
FGSGMPAERLNYIGQPFYSTKEKGTGLGLMICYRVVETHGGLIDIESKDKRGTNVKVILPMTTKNKAGSMVREKAAMLTY
G

Sequences:

>Translated_321_residues
MEIEELFGLLDLSEDCYFVHDRATILFANRAAHECLGKPIGYLHGKRLKQLISEEDIVCAGKPFQLGKKRLYASVLKQPF
KQAHKRKEAMEVVSRLSAGVAHELRNPLTTIKGFIQLYSEVGELRESQINIMNIELERIENIINDYVLLAEEPPARENES
IQMAAFLQSLLKKNKVKRLTDGHPIKLNISEEATVLGSRKELFLLFYNLLENAIDASVKHSPVHITMYAEGPNVCVEITD
FGSGMPAERLNYIGQPFYSTKEKGTGLGLMICYRVVETHGGLIDIESKDKRGTNVKVILPMTTKNKAGSMVREKAAMLTY
G
>Mature_321_residues
MEIEELFGLLDLSEDCYFVHDRATILFANRAAHECLGKPIGYLHGKRLKQLISEEDIVCAGKPFQLGKKRLYASVLKQPF
KQAHKRKEAMEVVSRLSAGVAHELRNPLTTIKGFIQLYSEVGELRESQINIMNIELERIENIINDYVLLAEEPPARENES
IQMAAFLQSLLKKNKVKRLTDGHPIKLNISEEATVLGSRKELFLLFYNLLENAIDASVKHSPVHITMYAEGPNVCVEITD
FGSGMPAERLNYIGQPFYSTKEKGTGLGLMICYRVVETHGGLIDIESKDKRGTNVKVILPMTTKNKAGSMVREKAAMLTY
G

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=29.2372881355932, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=28.448275862069, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788393, Length=237, Percent_Identity=24.4725738396624, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786912, Length=249, Percent_Identity=25.7028112449799, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786600, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790346, Length=222, Percent_Identity=25.6756756756757, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994928, Length=228, Percent_Identity=24.5614035087719, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081816, Length=237, Percent_Identity=28.2700421940928, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 36067; Mature: 36067

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIEELFGLLDLSEDCYFVHDRATILFANRAAHECLGKPIGYLHGKRLKQLISEEDIVCA
CCHHHHHHHHCCCCCEEEEECCEEEEEECHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEC
GKPFQLGKKRLYASVLKQPFKQAHKRKEAMEVVSRLSAGVAHELRNPLTTIKGFIQLYSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGELRESQINIMNIELERIENIINDYVLLAEEPPARENESIQMAAFLQSLLKKNKVKRLT
HHHHHHHCEEEEEEEHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
DGHPIKLNISEEATVLGSRKELFLLFYNLLENAIDASVKHSPVHITMYAEGPNVCVEITD
CCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEE
FGSGMPAERLNYIGQPFYSTKEKGTGLGLMICYRVVETHGGLIDIESKDKRGTNVKVILP
CCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEE
MTTKNKAGSMVREKAAMLTYG
ECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEIEELFGLLDLSEDCYFVHDRATILFANRAAHECLGKPIGYLHGKRLKQLISEEDIVCA
CCHHHHHHHHCCCCCEEEEECCEEEEEECHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEC
GKPFQLGKKRLYASVLKQPFKQAHKRKEAMEVVSRLSAGVAHELRNPLTTIKGFIQLYSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGELRESQINIMNIELERIENIINDYVLLAEEPPARENESIQMAAFLQSLLKKNKVKRLT
HHHHHHHCEEEEEEEHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
DGHPIKLNISEEATVLGSRKELFLLFYNLLENAIDASVKHSPVHITMYAEGPNVCVEITD
CCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEE
FGSGMPAERLNYIGQPFYSTKEKGTGLGLMICYRVVETHGGLIDIESKDKRGTNVKVILP
CCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEE
MTTKNKAGSMVREKAAMLTYG
ECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]