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The map label for this gene is greA

Identifier: 56963364

GI number: 56963364

Start: 1723503

End: 1723979

Strand: Direct

Name: greA

Synonym: ABC1599

Alternate gene names: 56963364

Gene position: 1723503-1723979 (Clockwise)

Preceding gene: 56963363

Following gene: 56963365

Centisome position: 40.05

GC content: 42.35

Gene sequence:

>477_bases
ATGGCAGAAGAGAAAAAACATTACATGACCGCAGAAGGCAAGAAAAAACTTGAAGAAGAACTACATTATTTGATTACAAC
ACGACGTAAAGAAGTCGTTGAACGTATAAAAGTCGCACGTAGCTTTGGCGACCTGTCGGAGAACTCCGAGTATGATTCTG
CCAAAGAAGACCAGGCATTTGTTGAAGGCCGGATTGCCCAGCTGGAAAAAATGATTCGCAATGCCGTCATGATTGAAAAT
GAAAAAGTAGATGGCAATGTTGTTTCATTAGGAAAAACAGTTCGCTTAATGGAACTGCCTGATGGGGAAGAAGAAGAATA
TACGATTGTCGGTTCAGCGGAGTCAGATCCTTTTGAGGGGAAAATTTCAAATGACTCTCCGATGGCGCAAAGTTTGTTAG
GTAAAACAGTTAATGACCGTGTAGTTGTGAATACGCCAGGCGGGGAAATGGAAGTCGAAATCCTTGAAATCCGCTAG

Upstream 100 bases:

>100_bases
GCAACAAAAATTCGCACAATTATTGAACAAAAGAAGTTGGATGCTTAAAAAAAGCCAGTCCCAACCGTCCTTTTGTATAT
ATTAATGAGGAGTGAATAGC

Downstream 100 bases:

>100_bases
ATATAAGAAGTTCTCTTGTTGCACTTGTAACAAGAGGACTCTTTTTTTTCACATGATTTTTCGTCAATCTCCGACAAAAG
GAAGGTTGAAGTTCAACTGA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAEEKKHYMTAEGKKKLEEELHYLITTRRKEVVERIKVARSFGDLSENSEYDSAKEDQAFVEGRIAQLEKMIRNAVMIEN
EKVDGNVVSLGKTVRLMELPDGEEEEYTIVGSAESDPFEGKISNDSPMAQSLLGKTVNDRVVVNTPGGEMEVEILEIR

Sequences:

>Translated_158_residues
MAEEKKHYMTAEGKKKLEEELHYLITTRRKEVVERIKVARSFGDLSENSEYDSAKEDQAFVEGRIAQLEKMIRNAVMIEN
EKVDGNVVSLGKTVRLMELPDGEEEEYTIVGSAESDPFEGKISNDSPMAQSLLGKTVNDRVVVNTPGGEMEVEILEIR
>Mature_157_residues
AEEKKHYMTAEGKKKLEEELHYLITTRRKEVVERIKVARSFGDLSENSEYDSAKEDQAFVEGRIAQLEKMIRNAVMIENE
KVDGNVVSLGKTVRLMELPDGEEEEYTIVGSAESDPFEGKISNDSPMAQSLLGKTVNDRVVVNTPGGEMEVEILEIR

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=150, Percent_Identity=44.6666666666667, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI87082261, Length=141, Percent_Identity=38.2978723404255, Blast_Score=83, Evalue=9e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_BACSK (Q5WHM1)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175095.1
- HSSP:   P0A6W5
- ProteinModelPortal:   Q5WHM1
- SMR:   Q5WHM1
- STRING:   Q5WHM1
- EnsemblBacteria:   EBBACT00000046768
- GeneID:   3204546
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1599
- NMPDR:   fig|66692.3.peg.1561
- eggNOG:   COG0782
- GeneTree:   EBGT00050000001871
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   BCLA66692:ABC1599-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17822; Mature: 17691

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEKKHYMTAEGKKKLEEELHYLITTRRKEVVERIKVARSFGDLSENSEYDSAKEDQAF
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
VEGRIAQLEKMIRNAVMIENEKVDGNVVSLGKTVRLMELPDGEEEEYTIVGSAESDPFEG
HHHHHHHHHHHHHHHHEECCCCCCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCCCCC
KISNDSPMAQSLLGKTVNDRVVVNTPGGEMEVEILEIR
EECCCCHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEC
>Mature Secondary Structure 
AEEKKHYMTAEGKKKLEEELHYLITTRRKEVVERIKVARSFGDLSENSEYDSAKEDQAF
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
VEGRIAQLEKMIRNAVMIENEKVDGNVVSLGKTVRLMELPDGEEEEYTIVGSAESDPFEG
HHHHHHHHHHHHHHHHEECCCCCCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCCCCC
KISNDSPMAQSLLGKTVNDRVVVNTPGGEMEVEILEIR
EECCCCHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA