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The map label for this gene is sacA [H]

Identifier: 56963220

GI number: 56963220

Start: 1574243

End: 1575622

Strand: Direct

Name: sacA [H]

Synonym: ABC1455

Alternate gene names: 56963220

Gene position: 1574243-1575622 (Clockwise)

Preceding gene: 56963219

Following gene: 56963221

Centisome position: 36.58

GC content: 47.39

Gene sequence:

>1380_bases
ATGAGTAAAAGTGACGCTTATAACTTGAAGTACCATATTACGCCGACACACGGCTTATTAAATGACCCGAACGGGCTTGC
CTATTTCCAAGGTCAGTATCATGTTTTTTATCAGTGGAATCCCTATGGCACCGAACATAAAAACAAAAGCTGGGGTCATG
TTGTTTCCGATGACCTAGTGAACTGGCATAGGAAAGAGACGGCATTGGTACCTTCGGAATGGTACGACAAGGACGGCATT
TATTCCGGCGGAAGCATTGTTCATGAAGGAAAGCTTTATCTCTTCTATACAGGTAACGTCATCAACAAAGATGGGACACG
GGCAAGTTATCAATGCGCGGCTGTTTCTGAAGACGGGCACCAGTTCCAGAAACTCGGTCCGTTATTCGAGCATCCTGCTG
GTTATACGAGACATGTCCGCGATCCGAAAGTCTGGAAAGACGATTCAGGCAACTGGTGGTTGATCGTAGGGGCCCAGCGG
GAAGATCTTACGGGAGATGCGCTTGTTTATAAGTCCGAAGACTTGATCAGCTGGTCGTGCCAGGGATCGTTTCTCGAACA
GGAGCATTCATTCGGATATATGTGGGAATGTCCTGATGTGCTCCAGTTTCCTGAACATGATGTCTTTGTCTTTTCCCCGC
AAGGCTTGCCAGAGGAAGGCGATCAATATAAAAATCCGAATCAATCAGGTTATATTATTGGGCGCATTTCAGAGTCCGGG
AAATTTCTTGGTACGTTGTCTGACTTCAACGAGCTCGACCGGGGATTTGATTTTTATGCCCCGCAAACGTTCAAGGTCAA
CAATCGAATCTTGATGTTCGGGTGGATGAGCGCAATGACGGAGGAAACAGAGCAAGCTGTCCCGACAATCGAGGAAGGAT
GGGTTCATGCATTGACTCTTCCGAGGGAAATCGTTTTCGCAGATGGCGTGCTATATCAGAGGCCGTTACATGAGCTCCAG
CTATTAAGGCAGCATGAAGTGCACGAAGGAATTAGCAACAGCGGTCAATGGAGCTTGCCTTCCCTAGCAGCTGAGGTCGT
TATTCGGTTTATGGAGCAACCTGACAGCTTCCAGCTCATCATCAGAAACTCCGTACAGATCGACTATGATCATGAGCAAA
ACAGTTTGACTGTCTGGAGAACGAACTGGTTGACGAACGAGAAGGAACACCGTAGAGCTGTGCTCGGTCATTCGCTTGCA
GAATTGCACATGTTTATTGAAAGCTCCAGCATAGAGCTATTTGTGAATGAAGGGGAGGAAGTATTCTCTTTGCGTTACTT
TCTAGAGGATACGAACGAGCGCAGCGTGCGGTTTGAGTCAGACTGCAGCGAAGCTGTCTTAAGTATGTACCGCTTGCAAG
AAAGTATAGACGAGGAGTGA

Upstream 100 bases:

>100_bases
AAGTTCGAGAGCGAGCCGGGGGATTTTGAGGTTTATATCGGCACAAGCTCACAAGAAACGCCGCTGACGTTCAACTTCGC
TTTACGGGCGGGGGAACATG

Downstream 100 bases:

>100_bases
CTTATCACTTTCCATCGCTCAAAAACGCCATGATAGGCTTGTCAGCGATTAGCCGGTAATTGTTCCCAATATATTTGAAC
CTGCTGGAAGTATAAAGATA

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSKSDAYNLKYHITPTHGLLNDPNGLAYFQGQYHVFYQWNPYGTEHKNKSWGHVVSDDLVNWHRKETALVPSEWYDKDGI
YSGGSIVHEGKLYLFYTGNVINKDGTRASYQCAAVSEDGHQFQKLGPLFEHPAGYTRHVRDPKVWKDDSGNWWLIVGAQR
EDLTGDALVYKSEDLISWSCQGSFLEQEHSFGYMWECPDVLQFPEHDVFVFSPQGLPEEGDQYKNPNQSGYIIGRISESG
KFLGTLSDFNELDRGFDFYAPQTFKVNNRILMFGWMSAMTEETEQAVPTIEEGWVHALTLPREIVFADGVLYQRPLHELQ
LLRQHEVHEGISNSGQWSLPSLAAEVVIRFMEQPDSFQLIIRNSVQIDYDHEQNSLTVWRTNWLTNEKEHRRAVLGHSLA
ELHMFIESSSIELFVNEGEEVFSLRYFLEDTNERSVRFESDCSEAVLSMYRLQESIDEE

Sequences:

>Translated_459_residues
MSKSDAYNLKYHITPTHGLLNDPNGLAYFQGQYHVFYQWNPYGTEHKNKSWGHVVSDDLVNWHRKETALVPSEWYDKDGI
YSGGSIVHEGKLYLFYTGNVINKDGTRASYQCAAVSEDGHQFQKLGPLFEHPAGYTRHVRDPKVWKDDSGNWWLIVGAQR
EDLTGDALVYKSEDLISWSCQGSFLEQEHSFGYMWECPDVLQFPEHDVFVFSPQGLPEEGDQYKNPNQSGYIIGRISESG
KFLGTLSDFNELDRGFDFYAPQTFKVNNRILMFGWMSAMTEETEQAVPTIEEGWVHALTLPREIVFADGVLYQRPLHELQ
LLRQHEVHEGISNSGQWSLPSLAAEVVIRFMEQPDSFQLIIRNSVQIDYDHEQNSLTVWRTNWLTNEKEHRRAVLGHSLA
ELHMFIESSSIELFVNEGEEVFSLRYFLEDTNERSVRFESDCSEAVLSMYRLQESIDEE
>Mature_458_residues
SKSDAYNLKYHITPTHGLLNDPNGLAYFQGQYHVFYQWNPYGTEHKNKSWGHVVSDDLVNWHRKETALVPSEWYDKDGIY
SGGSIVHEGKLYLFYTGNVINKDGTRASYQCAAVSEDGHQFQKLGPLFEHPAGYTRHVRDPKVWKDDSGNWWLIVGAQRE
DLTGDALVYKSEDLISWSCQGSFLEQEHSFGYMWECPDVLQFPEHDVFVFSPQGLPEEGDQYKNPNQSGYIIGRISESGK
FLGTLSDFNELDRGFDFYAPQTFKVNNRILMFGWMSAMTEETEQAVPTIEEGWVHALTLPREIVFADGVLYQRPLHELQL
LRQHEVHEGISNSGQWSLPSLAAEVVIRFMEQPDSFQLIIRNSVQIDYDHEQNSLTVWRTNWLTNEKEHRRAVLGHSLAE
LHMFIESSSIELFVNEGEEVFSLRYFLEDTNERSVRFESDCSEAVLSMYRLQESIDEE

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=491, Percent_Identity=27.2912423625255, Blast_Score=106, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 53081; Mature: 52950

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSDAYNLKYHITPTHGLLNDPNGLAYFQGQYHVFYQWNPYGTEHKNKSWGHVVSDDLV
CCCCCCEEEEEEEECCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCCCCHHHHHH
NWHRKETALVPSEWYDKDGIYSGGSIVHEGKLYLFYTGNVINKDGTRASYQCAAVSEDGH
HHHHHCCEECCHHHCCCCCCCCCCCEEECCEEEEEEECCEECCCCCCCEEEEEEECCCCH
QFQKLGPLFEHPAGYTRHVRDPKVWKDDSGNWWLIVGAQREDLTGDALVYKSEDLISWSC
HHHHHCCHHHCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCCCEEEEECCCEEEEEC
QGSFLEQEHSFGYMWECPDVLQFPEHDVFVFSPQGLPEEGDQYKNPNQSGYIIGRISESG
CCCHHHHCCCCCEEECCCHHHHCCCCCEEEECCCCCCCCCHHCCCCCCCCEEEEEECCCC
KFLGTLSDFNELDRGFDFYAPQTFKVNNRILMFGWMSAMTEETEQAVPTIEEGWVHALTL
CEEEEHHHHHHHHCCCCCCCCEEEEECCEEEEEEEHHHHHHHHHHHCCCHHHCCEEEEEC
PREIVFADGVLYQRPLHELQLLRQHEVHEGISNSGQWSLPSLAAEVVIRFMEQPDSFQLI
CCEEEEECCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
IRNSVQIDYDHEQNSLTVWRTNWLTNEKEHRRAVLGHSLAELHMFIESSSIELFVNEGEE
EECCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCC
VFSLRYFLEDTNERSVRFESDCSEAVLSMYRLQESIDEE
EEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKSDAYNLKYHITPTHGLLNDPNGLAYFQGQYHVFYQWNPYGTEHKNKSWGHVVSDDLV
CCCCCEEEEEEEECCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCCCCHHHHHH
NWHRKETALVPSEWYDKDGIYSGGSIVHEGKLYLFYTGNVINKDGTRASYQCAAVSEDGH
HHHHHCCEECCHHHCCCCCCCCCCCEEECCEEEEEEECCEECCCCCCCEEEEEEECCCCH
QFQKLGPLFEHPAGYTRHVRDPKVWKDDSGNWWLIVGAQREDLTGDALVYKSEDLISWSC
HHHHHCCHHHCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCCCEEEEECCCEEEEEC
QGSFLEQEHSFGYMWECPDVLQFPEHDVFVFSPQGLPEEGDQYKNPNQSGYIIGRISESG
CCCHHHHCCCCCEEECCCHHHHCCCCCEEEECCCCCCCCCHHCCCCCCCCEEEEEECCCC
KFLGTLSDFNELDRGFDFYAPQTFKVNNRILMFGWMSAMTEETEQAVPTIEEGWVHALTL
CEEEEHHHHHHHHCCCCCCCCEEEEECCEEEEEEEHHHHHHHHHHHCCCHHHCCEEEEEC
PREIVFADGVLYQRPLHELQLLRQHEVHEGISNSGQWSLPSLAAEVVIRFMEQPDSFQLI
CCEEEEECCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
IRNSVQIDYDHEQNSLTVWRTNWLTNEKEHRRAVLGHSLAELHMFIESSSIELFVNEGEE
EECCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCC
VFSLRYFLEDTNERSVRFESDCSEAVLSMYRLQESIDEE
EEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3100393; 7934828; 9384377; 3122206 [H]