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The map label for this gene is nos [H]

Identifier: 56963039

GI number: 56963039

Start: 1388004

End: 1389095

Strand: Direct

Name: nos [H]

Synonym: ABC1267

Alternate gene names: 56963039

Gene position: 1388004-1389095 (Clockwise)

Preceding gene: 56963038

Following gene: 56963040

Centisome position: 32.25

GC content: 49.82

Gene sequence:

>1092_bases
ATGGCAAATCGTTTGGAAGATGAGGCTGTTGAGTTTCTTTCCCTTTTTTACGAGGAGCGTTGCTTAGACGGCAAAGAAGC
GCGGGTTCGCGACGTTTGCGAAGCGATCCGTACAGGCGGCACTTACGAGCATACGGCTGAGGAGCTTGCATTTGGGGCGA
GGCTCGCTTGGCGTAACAGCAATCGTTGCATTGGCCGCTTATTTTGGGAACGGCTTACCGTTATTGATGAACGGGAAGCA
CGTACAGAAGAAGCGGTCGCACAAGCGCTTTTTAGGCATATTGAATATGCGACAAACGGTGGAAAAGTCATTCCGACGAT
TACGGTGTTTAAACAAGGGGCTTCAATCCGCATTTGGAATGCGCAACTTGTCCGTTATGCAGGCTATCGCGGTGATGGCC
GCATTATTGGCGATCCTGCTTCCCTTGAATTAACGGACATTTGCAAGCAGCTTGGCTGGGTTGGTGCAGGGGGCGATTTT
GATGTGCTACCCCTTGTCATATCGGTGGGTGCACGTCCCCCTCAGCTATTCCCTATCCCCGAGCATCTGATTAAAGAAGT
GGCCCTTGTCCATCCTGATAACGAAGCATTTGCTAGGCTGAACCTTAAATGGTATGCAGTGCCGTTTATATCAAATATGC
GCCTTGAAATTGGCGGCATTTCTTATTACACAGCGCCGTTTAACGGTTGGTATATGGGCACAGAAATCGGCGCCCGCAAT
TTAGCCGATGATTTCCGCTATAACCGCTTGCCAGAAGTGGCTGCCGTGCTCGGAATCAGCATGGAGCGTGAAGCATCCCT
GTGGCGCGATCAAGCGCTTGTTGAATTAAACCGTGCTGTTCTCTATTCGTATCAGGAACAAGGCGTAAGCATTGTCGATC
ACCATACTGCCGCAAAACAGTTTCAACGTTTTATTGGCAATGAAACAGAAGCAGGGCGCGACGTGACAGGCGACTGGACG
TGGCTAATCCCGCCGATGTCCCCAGCGCAGACGGATATTTTTCACCAGTCGTTTGATAATCGCATCGTTTCGCCGAATTA
CTTCCACGAAAAGCCACCCTATTGTAGGAAAGGGATAAAAGAAGAATATTAA

Upstream 100 bases:

>100_bases
GAAACGATTGAAGCAGATGTCATCGAAGAACTGAAGGCCGATGCATAGAAAAGGCTGTGCAGCATAGGACGCTGCACAGT
TTTTTTAGGAGGATGGCAAT

Downstream 100 bases:

>100_bases
AGGAAGATGCACATGAGTTTACTTCGAGCAGAAGGGCTAACAAAGTCTTTTGGCGACAAGATTTGTTTTGAATCGATTCA
CTTTAGTTTAGAAAAAGGCG

Product: nitric-oxide synthase oxygenase subunit

Products: citrulline; nitric oxide; NADP+

Alternate protein names: NOSoxy-like protein [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MANRLEDEAVEFLSLFYEERCLDGKEARVRDVCEAIRTGGTYEHTAEELAFGARLAWRNSNRCIGRLFWERLTVIDEREA
RTEEAVAQALFRHIEYATNGGKVIPTITVFKQGASIRIWNAQLVRYAGYRGDGRIIGDPASLELTDICKQLGWVGAGGDF
DVLPLVISVGARPPQLFPIPEHLIKEVALVHPDNEAFARLNLKWYAVPFISNMRLEIGGISYYTAPFNGWYMGTEIGARN
LADDFRYNRLPEVAAVLGISMEREASLWRDQALVELNRAVLYSYQEQGVSIVDHHTAAKQFQRFIGNETEAGRDVTGDWT
WLIPPMSPAQTDIFHQSFDNRIVSPNYFHEKPPYCRKGIKEEY

Sequences:

>Translated_363_residues
MANRLEDEAVEFLSLFYEERCLDGKEARVRDVCEAIRTGGTYEHTAEELAFGARLAWRNSNRCIGRLFWERLTVIDEREA
RTEEAVAQALFRHIEYATNGGKVIPTITVFKQGASIRIWNAQLVRYAGYRGDGRIIGDPASLELTDICKQLGWVGAGGDF
DVLPLVISVGARPPQLFPIPEHLIKEVALVHPDNEAFARLNLKWYAVPFISNMRLEIGGISYYTAPFNGWYMGTEIGARN
LADDFRYNRLPEVAAVLGISMEREASLWRDQALVELNRAVLYSYQEQGVSIVDHHTAAKQFQRFIGNETEAGRDVTGDWT
WLIPPMSPAQTDIFHQSFDNRIVSPNYFHEKPPYCRKGIKEEY
>Mature_362_residues
ANRLEDEAVEFLSLFYEERCLDGKEARVRDVCEAIRTGGTYEHTAEELAFGARLAWRNSNRCIGRLFWERLTVIDEREAR
TEEAVAQALFRHIEYATNGGKVIPTITVFKQGASIRIWNAQLVRYAGYRGDGRIIGDPASLELTDICKQLGWVGAGGDFD
VLPLVISVGARPPQLFPIPEHLIKEVALVHPDNEAFARLNLKWYAVPFISNMRLEIGGISYYTAPFNGWYMGTEIGARNL
ADDFRYNRLPEVAAVLGISMEREASLWRDQALVELNRAVLYSYQEQGVSIVDHHTAAKQFQRFIGNETEAGRDVTGDWTW
LIPPMSPAQTDIFHQSFDNRIVSPNYFHEKPPYCRKGIKEEY

Specific function: Catalyzes the production of nitric oxide [H]

COG id: COG4362

COG function: function code PE; Nitric oxide synthase, oxygenase domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NOS family. Bacterial NOS oxygenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=356, Percent_Identity=46.6292134831461, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI231571356, Length=359, Percent_Identity=45.125348189415, Blast_Score=328, Evalue=7e-90,
Organism=Homo sapiens, GI231571314, Length=359, Percent_Identity=45.125348189415, Blast_Score=328, Evalue=7e-90,
Organism=Homo sapiens, GI231571329, Length=359, Percent_Identity=45.125348189415, Blast_Score=327, Evalue=8e-90,
Organism=Homo sapiens, GI40254422, Length=359, Percent_Identity=45.125348189415, Blast_Score=327, Evalue=9e-90,
Organism=Homo sapiens, GI10835173, Length=354, Percent_Identity=44.0677966101695, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI78706866, Length=356, Percent_Identity=42.6966292134831, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI78706868, Length=356, Percent_Identity=42.6966292134831, Blast_Score=317, Evalue=5e-87,
Organism=Drosophila melanogaster, GI78706882, Length=356, Percent_Identity=42.6966292134831, Blast_Score=317, Evalue=7e-87,
Organism=Drosophila melanogaster, GI78706870, Length=356, Percent_Identity=42.6966292134831, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI78706878, Length=356, Percent_Identity=42.6966292134831, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI78706880, Length=356, Percent_Identity=42.6966292134831, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24583543, Length=332, Percent_Identity=44.8795180722892, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI78706876, Length=332, Percent_Identity=44.8795180722892, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI78706872, Length=126, Percent_Identity=47.6190476190476, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017142
- InterPro:   IPR004030 [H]

Pfam domain/function: PF02898 NO_synthase [H]

EC number: 1.14.13.39

Molecular weight: Translated: 41296; Mature: 41165

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS60001 NOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRLEDEAVEFLSLFYEERCLDGKEARVRDVCEAIRTGGTYEHTAEELAFGARLAWRNS
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEECCC
NRCIGRLFWERLTVIDEREARTEEAVAQALFRHIEYATNGGKVIPTITVFKQGASIRIWN
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEC
AQLVRYAGYRGDGRIIGDPASLELTDICKQLGWVGAGGDFDVLPLVISVGARPPQLFPIP
HHHHHHCCCCCCCCEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCH
EHLIKEVALVHPDNEAFARLNLKWYAVPFISNMRLEIGGISYYTAPFNGWYMGTEIGARN
HHHHHHHHCCCCCCHHEEEEEEEEEEEECCCCCEEEECCEEEEECCCCCEEECCHHCCHH
LADDFRYNRLPEVAAVLGISMEREASLWRDQALVELNRAVLYSYQEQGVSIVDHHTAAKQ
HHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHH
FQRFIGNETEAGRDVTGDWTWLIPPMSPAQTDIFHQSFDNRIVSPNYFHEKPPYCRKGIK
HHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCC
EEY
CCC
>Mature Secondary Structure 
ANRLEDEAVEFLSLFYEERCLDGKEARVRDVCEAIRTGGTYEHTAEELAFGARLAWRNS
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEECCC
NRCIGRLFWERLTVIDEREARTEEAVAQALFRHIEYATNGGKVIPTITVFKQGASIRIWN
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEC
AQLVRYAGYRGDGRIIGDPASLELTDICKQLGWVGAGGDFDVLPLVISVGARPPQLFPIP
HHHHHHCCCCCCCCEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCH
EHLIKEVALVHPDNEAFARLNLKWYAVPFISNMRLEIGGISYYTAPFNGWYMGTEIGARN
HHHHHHHHCCCCCCHHEEEEEEEEEEEECCCCCEEEECCEEEEECCCCCEEECCHHCCHH
LADDFRYNRLPEVAAVLGISMEREASLWRDQALVELNRAVLYSYQEQGVSIVDHHTAAKQ
HHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHH
FQRFIGNETEAGRDVTGDWTWLIPPMSPAQTDIFHQSFDNRIVSPNYFHEKPPYCRKGIK
HHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCC
EEY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-arginine; NADPH; H+; O2

Specific reaction: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 11856757; 12220171 [H]