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The map label for this gene is alsT [H]

Identifier: 56963031

GI number: 56963031

Start: 1376926

End: 1378314

Strand: Direct

Name: alsT [H]

Synonym: ABC1259

Alternate gene names: 56963031

Gene position: 1376926-1378314 (Clockwise)

Preceding gene: 56963030

Following gene: 56963032

Centisome position: 31.99

GC content: 46.58

Gene sequence:

>1389_bases
ATGGAAGTTGTTCAACAAATCGTAGATAGCATGAACGGGATTACTTCCTATTTTCTCGTGTTTTCTTTAGTTGCCGTTGG
CCTGTACTTAACGTTCCGGTCAAACTTTGTGCAAATCCGGATGTTTCCTGAAATGTTTCGAGCTGTCACCGAGAAAAAGA
CGAAGAAGGGAGAAGTCTCTGCTTTTCAAGCGTTTTGTATTAGCGCCGCTTCGCGAGTAGGCGCAGCCAACATCTCAGGG
GTGGCGATTGCGATTACAACAGGAGGCCCTGGCGCGATTTTTTGGATGTGGGTGATTGCGACCATCGGCATGGCTACTGG
CTTTGTAGAAAGCATGCTTGCCCAAGTGTACAAAGAAAGGCGCGACGGCCAGTTCCGAGGCGGCCCTGCTTATTATATTG
AAAAGGCCCTTGGCAAACGCTGGCTTGGCATCGTATTTGCCTTATTGATTGCGGTCACATTTGGATTTATCTTTAATTCA
CTACAAGCGAATACGATTGCTGTTGCTATGGATGAAGCATTTGGGCTAAACCGACTAACTGTCGGCATCGTCATTGCTCT
AGCAGTAGGGGTTATTATCTTTGGCGGCATTCGGTCGATTTCTGTCTTTTCAGGAATTGTTGTGCCGATTATGGCAGTGG
CCTATGTCGGTGTTGTCTTTTTTGTGGTGATCAGCAATTATCAACAAATTCCGCAAGTATTTGCTCTTATTATCGGTTCT
GCCTTTGGGTTTAAAGAGGCGACAGTCGGCATTTTTGCCGGGCTCCTTGTCGGGGCGCAGAGAGGCTTGTTTTCCAATGA
AGCTGGAATGGGAAGTGTCCCGAACGCCGCCGCGACAGCCGATGTATCCCATCCAGTCAAGCAAGGGCTTGTGCAATCTT
TAGGTGTGTTTTTTGATACGATTATCATTTGTTCTGCGACTGCTTTTGTTATTTTGCTTACGAATGTTTACCAAACCGCT
GGAGAAGGGGTAGACGGCGTCGCCTTAACACAAGCGTCCTTGAGCGAGCTCATCGGCGGTTGGGCTGTCCCATTTATTGC
GATTGCGATTTTCTTTTTTTCGTTCACTTCGCTCGTCGGCAACTACTACTACGGCGAAACCAATTTAGCATTTATTAAAG
AGGGGACCATTTGGCTCCAAGTTTACCGTTTGCTCGTCGTCGCTATGGTTGTTTTCGGTTCCCTTGCTGCGATTGAACTC
GTTTGGTCTTTAGCTGATGTGTTTATGGCCTTTACGACCGTGGTGAACTTAATGGCGATTCTATTAATCGGCGGGATTTC
CTTTGCGGTTATTTCGGATTACCAGAGCCAGCGTCAACAAGGAAAAAATCCTGTTTTTAAAAGCAAAAACATTCCCGGTT
TAAAAAATGCCAGCACATGGGATGATTGA

Upstream 100 bases:

>100_bases
AAAAAGTCTAAATAGGGCGTTTTCTTTTAAATAGCAAAAAATGTGATATGATGCTTAAGGAAAAAACCTTATTTTTGGGA
GAAGCAAAGGTGGAGCTATT

Downstream 100 bases:

>100_bases
TAACGAAAAGAGGCCTGCACTCACCTGAGCGCAGGCCTTTTTTATGCTTTCAAAAACATACCCATATTTTAATATCTTGT
CTCATAGCCTGTATAGAGAG

Product: sodium:alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MEVVQQIVDSMNGITSYFLVFSLVAVGLYLTFRSNFVQIRMFPEMFRAVTEKKTKKGEVSAFQAFCISAASRVGAANISG
VAIAITTGGPGAIFWMWVIATIGMATGFVESMLAQVYKERRDGQFRGGPAYYIEKALGKRWLGIVFALLIAVTFGFIFNS
LQANTIAVAMDEAFGLNRLTVGIVIALAVGVIIFGGIRSISVFSGIVVPIMAVAYVGVVFFVVISNYQQIPQVFALIIGS
AFGFKEATVGIFAGLLVGAQRGLFSNEAGMGSVPNAAATADVSHPVKQGLVQSLGVFFDTIIICSATAFVILLTNVYQTA
GEGVDGVALTQASLSELIGGWAVPFIAIAIFFFSFTSLVGNYYYGETNLAFIKEGTIWLQVYRLLVVAMVVFGSLAAIEL
VWSLADVFMAFTTVVNLMAILLIGGISFAVISDYQSQRQQGKNPVFKSKNIPGLKNASTWDD

Sequences:

>Translated_462_residues
MEVVQQIVDSMNGITSYFLVFSLVAVGLYLTFRSNFVQIRMFPEMFRAVTEKKTKKGEVSAFQAFCISAASRVGAANISG
VAIAITTGGPGAIFWMWVIATIGMATGFVESMLAQVYKERRDGQFRGGPAYYIEKALGKRWLGIVFALLIAVTFGFIFNS
LQANTIAVAMDEAFGLNRLTVGIVIALAVGVIIFGGIRSISVFSGIVVPIMAVAYVGVVFFVVISNYQQIPQVFALIIGS
AFGFKEATVGIFAGLLVGAQRGLFSNEAGMGSVPNAAATADVSHPVKQGLVQSLGVFFDTIIICSATAFVILLTNVYQTA
GEGVDGVALTQASLSELIGGWAVPFIAIAIFFFSFTSLVGNYYYGETNLAFIKEGTIWLQVYRLLVVAMVVFGSLAAIEL
VWSLADVFMAFTTVVNLMAILLIGGISFAVISDYQSQRQQGKNPVFKSKNIPGLKNASTWDD
>Mature_462_residues
MEVVQQIVDSMNGITSYFLVFSLVAVGLYLTFRSNFVQIRMFPEMFRAVTEKKTKKGEVSAFQAFCISAASRVGAANISG
VAIAITTGGPGAIFWMWVIATIGMATGFVESMLAQVYKERRDGQFRGGPAYYIEKALGKRWLGIVFALLIAVTFGFIFNS
LQANTIAVAMDEAFGLNRLTVGIVIALAVGVIIFGGIRSISVFSGIVVPIMAVAYVGVVFFVVISNYQQIPQVFALIIGS
AFGFKEATVGIFAGLLVGAQRGLFSNEAGMGSVPNAAATADVSHPVKQGLVQSLGVFFDTIIICSATAFVILLTNVYQTA
GEGVDGVALTQASLSELIGGWAVPFIAIAIFFFSFTSLVGNYYYGETNLAFIKEGTIWLQVYRLLVVAMVVFGSLAAIEL
VWSLADVFMAFTTVVNLMAILLIGGISFAVISDYQSQRQQGKNPVFKSKNIPGLKNASTWDD

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=464, Percent_Identity=39.6551724137931, Blast_Score=323, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49527; Mature: 49527

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVVQQIVDSMNGITSYFLVFSLVAVGLYLTFRSNFVQIRMFPEMFRAVTEKKTKKGEVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCCHHH
AFQAFCISAASRVGAANISGVAIAITTGGPGAIFWMWVIATIGMATGFVESMLAQVYKER
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDGQFRGGPAYYIEKALGKRWLGIVFALLIAVTFGFIFNSLQANTIAVAMDEAFGLNRLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCHHHH
VGIVIALAVGVIIFGGIRSISVFSGIVVPIMAVAYVGVVFFVVISNYQQIPQVFALIIGS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGFKEATVGIFAGLLVGAQRGLFSNEAGMGSVPNAAATADVSHPVKQGLVQSLGVFFDT
HCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHH
IIICSATAFVILLTNVYQTAGEGVDGVALTQASLSELIGGWAVPFIAIAIFFFSFTSLVG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NYYYGETNLAFIKEGTIWLQVYRLLVVAMVVFGSLAAIELVWSLADVFMAFTTVVNLMAI
CCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLIGGISFAVISDYQSQRQQGKNPVFKSKNIPGLKNASTWDD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEVVQQIVDSMNGITSYFLVFSLVAVGLYLTFRSNFVQIRMFPEMFRAVTEKKTKKGEVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCCHHH
AFQAFCISAASRVGAANISGVAIAITTGGPGAIFWMWVIATIGMATGFVESMLAQVYKER
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDGQFRGGPAYYIEKALGKRWLGIVFALLIAVTFGFIFNSLQANTIAVAMDEAFGLNRLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCHHHH
VGIVIALAVGVIIFGGIRSISVFSGIVVPIMAVAYVGVVFFVVISNYQQIPQVFALIIGS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGFKEATVGIFAGLLVGAQRGLFSNEAGMGSVPNAAATADVSHPVKQGLVQSLGVFFDT
HCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHH
IIICSATAFVILLTNVYQTAGEGVDGVALTQASLSELIGGWAVPFIAIAIFFFSFTSLVG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NYYYGETNLAFIKEGTIWLQVYRLLVVAMVVFGSLAAIELVWSLADVFMAFTTVVNLMAI
CCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLIGGISFAVISDYQSQRQQGKNPVFKSKNIPGLKNASTWDD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]