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The map label for this gene is gsiB [H]

Identifier: 56962991

GI number: 56962991

Start: 1329268

End: 1330869

Strand: Direct

Name: gsiB [H]

Synonym: ABC1219

Alternate gene names: 56962991

Gene position: 1329268-1330869 (Clockwise)

Preceding gene: 56962990

Following gene: 56962993

Centisome position: 30.89

GC content: 46.94

Gene sequence:

>1602_bases
ATGTTTAAGAAAAACGCTTTATGGTTAGCGGTTGCAGCTTCTGCCATGCTTGTCGTGACTGCATGCTCAAACGACCCTGA
ACAAGCAGAAAATACAAGCGATGAGGCGGAAAACAGCGCAAGCGGGGCCGAGCTAACGATTGATTTGTCGTCTGATCCTG
TTTCTCTCGACCCCCATGGGGCAAATGATGGGAATTCATTATATGTAATGGGAACTCTTTATAATAAGTTGGTTGAGTTT
GATGAAAACAATGAGATTCAACCAAGCTTGGCGACAAGCCTTGAACAAATAGAAGATGATGTCTGGGAAGCAAAGTTGCG
TGAAGACGTGACATTCCACGACGGCAGTGCATTTAATGCTGAAGTCGTTAAAGCCAACTTGGACCGTGTTCGCGACCCGG
ATCTAGGTTCCCCTGTTGCGTTTTTGTTTGATAAAATCACCGATGTTGAAGTCATCGATGAGTACACGGTTCATATCCAT
ACAGACGGCCCATTTTCGCCGCTTCCGTCCCATTTAGCTCACCCAGCTGGTGGAATGATTAGCAAAGAAGCGATTGACGC
TGATTATGAAGCAATCGAAAACGGCGAACCTGCTTTTTCATACGTAGATGCACATCCGATCGGCACTGGTTTTTTTGAAT
TGGACAGCCGTACATCAGGCGAATCGGTCTCTGTAACCCGGAATGAAGATTACTGGGACAAGGGCAATCTTGCTAAAGCA
AGCGGGATTACCTTTAAAACGGTTCCAGAGGAAGCCACGCGAATTGCTGAGTTGCAAGGCGATGTAGCGGATATTATTTA
CCCAGTTAACCCGAGCAGCATTTCGGAAATTGAGTCTGCCGAAAACACGACTGTGAAACAGTCCCCGAGCTCGAACTTGA
CTTATCTAGGCTTTAACACTGAAGTTGAACCGTTTACGGATAAGCGAGTACGCCAAGCCATTAATATGGCGATTGACAAA
GACAGCATTATTAACGGCTTATTGGACGGCGTTGCTGAACCGGCGATTGGGCCACTTGCTCCTACTGTTACAGGTTACAG
CCAAAACATCGATACGTTCGAATACGACCAAGAAGAAGCGCGGCAGCTACTGGCAGAAGCAGGCTACGAAGACGGTTTTG
ATGCGACATTAACTGTCAACAGCAGCACTGCCTCATTAGTTGATTTGGCTACCTACTTGCAAGCCCAACTTGAAGAAATT
GGCATTCATGTTTCCGTTAATCCGGTTGAGAGTGCTACGTATTTGGAAATGACCGGCGATGGCGAGACGGAAATGTTTAT
CGGCACATGGGGAACCGTAACTGTCGATGCCGATTATGGCTTGTACCCGATGTTCCATTCGTCCAACTTTGGCGCCCCTG
GCAACAGAACGTTCTTTGCCAATGAGCAATTAGACGATTTGCTAGAGCAAGCGCGTAAAGAGACGGATGAACAAAAGCGT
TTGGCCCTTTATGAAGAAGCGCAAAACGTGTTGGCCGAGGAAGCGCCAGTCGTCAATTTGTACCACTCTGTGTTATTGGC
CGGCATGGGCGATGACATTGAAGGGTTCTGGCAATATCCAGCAAGTATTTTCTATTTACGTGATACGGAAAAAGTCGAGT
AA

Upstream 100 bases:

>100_bases
AGGCGAGAAATGATGGACTTCACCAAACCCTTTTATAAAAAGTCTAGTAATCTTATGGGAGATTAAGTATAATAGGGGAA
ACAACAAAAGAGGTGACATC

Downstream 100 bases:

>100_bases
TTTAATTGGCTAGTAAATGGCTATAAAAGCGGCGCGCTGCGATTGACAGTGGCGCTGCTTTTTTTGTAATGAGCGCGATT
TCCCTAGTAGGGATGGCTAT

Product: oligopeptide ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MFKKNALWLAVAASAMLVVTACSNDPEQAENTSDEAENSASGAELTIDLSSDPVSLDPHGANDGNSLYVMGTLYNKLVEF
DENNEIQPSLATSLEQIEDDVWEAKLREDVTFHDGSAFNAEVVKANLDRVRDPDLGSPVAFLFDKITDVEVIDEYTVHIH
TDGPFSPLPSHLAHPAGGMISKEAIDADYEAIENGEPAFSYVDAHPIGTGFFELDSRTSGESVSVTRNEDYWDKGNLAKA
SGITFKTVPEEATRIAELQGDVADIIYPVNPSSISEIESAENTTVKQSPSSNLTYLGFNTEVEPFTDKRVRQAINMAIDK
DSIINGLLDGVAEPAIGPLAPTVTGYSQNIDTFEYDQEEARQLLAEAGYEDGFDATLTVNSSTASLVDLATYLQAQLEEI
GIHVSVNPVESATYLEMTGDGETEMFIGTWGTVTVDADYGLYPMFHSSNFGAPGNRTFFANEQLDDLLEQARKETDEQKR
LALYEEAQNVLAEEAPVVNLYHSVLLAGMGDDIEGFWQYPASIFYLRDTEKVE

Sequences:

>Translated_533_residues
MFKKNALWLAVAASAMLVVTACSNDPEQAENTSDEAENSASGAELTIDLSSDPVSLDPHGANDGNSLYVMGTLYNKLVEF
DENNEIQPSLATSLEQIEDDVWEAKLREDVTFHDGSAFNAEVVKANLDRVRDPDLGSPVAFLFDKITDVEVIDEYTVHIH
TDGPFSPLPSHLAHPAGGMISKEAIDADYEAIENGEPAFSYVDAHPIGTGFFELDSRTSGESVSVTRNEDYWDKGNLAKA
SGITFKTVPEEATRIAELQGDVADIIYPVNPSSISEIESAENTTVKQSPSSNLTYLGFNTEVEPFTDKRVRQAINMAIDK
DSIINGLLDGVAEPAIGPLAPTVTGYSQNIDTFEYDQEEARQLLAEAGYEDGFDATLTVNSSTASLVDLATYLQAQLEEI
GIHVSVNPVESATYLEMTGDGETEMFIGTWGTVTVDADYGLYPMFHSSNFGAPGNRTFFANEQLDDLLEQARKETDEQKR
LALYEEAQNVLAEEAPVVNLYHSVLLAGMGDDIEGFWQYPASIFYLRDTEKVE
>Mature_533_residues
MFKKNALWLAVAASAMLVVTACSNDPEQAENTSDEAENSASGAELTIDLSSDPVSLDPHGANDGNSLYVMGTLYNKLVEF
DENNEIQPSLATSLEQIEDDVWEAKLREDVTFHDGSAFNAEVVKANLDRVRDPDLGSPVAFLFDKITDVEVIDEYTVHIH
TDGPFSPLPSHLAHPAGGMISKEAIDADYEAIENGEPAFSYVDAHPIGTGFFELDSRTSGESVSVTRNEDYWDKGNLAKA
SGITFKTVPEEATRIAELQGDVADIIYPVNPSSISEIESAENTTVKQSPSSNLTYLGFNTEVEPFTDKRVRQAINMAIDK
DSIINGLLDGVAEPAIGPLAPTVTGYSQNIDTFEYDQEEARQLLAEAGYEDGFDATLTVNSSTASLVDLATYLQAQLEEI
GIHVSVNPVESATYLEMTGDGETEMFIGTWGTVTVDADYGLYPMFHSSNFGAPGNRTFFANEQLDDLLEQARKETDEQKR
LALYEEAQNVLAEEAPVVNLYHSVLLAGMGDDIEGFWQYPASIFYLRDTEKVE

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=506, Percent_Identity=32.6086956521739, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1789966, Length=467, Percent_Identity=28.4796573875803, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787762, Length=483, Percent_Identity=27.1221532091097, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI87081878, Length=496, Percent_Identity=26.2096774193548, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789887, Length=484, Percent_Identity=26.6528925619835, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789397, Length=543, Percent_Identity=23.3885819521179, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1787551, Length=470, Percent_Identity=23.6170212765957, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787495, Length=480, Percent_Identity=24.375, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58235; Mature: 58235

Theoretical pI: Translated: 3.84; Mature: 3.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKNALWLAVAASAMLVVTACSNDPEQAENTSDEAENSASGAELTIDLSSDPVSLDPHG
CCCCCEEEEEEHHCEEEEEEECCCCHHHHCCCCHHHHCCCCCCEEEEEECCCCCCCCCCC
ANDGNSLYVMGTLYNKLVEFDENNEIQPSLATSLEQIEDDVWEAKLREDVTFHDGSAFNA
CCCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCH
EVVKANLDRVRDPDLGSPVAFLFDKITDVEVIDEYTVHIHTDGPFSPLPSHLAHPAGGMI
HHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCHHHCCCCCCCC
SKEAIDADYEAIENGEPAFSYVDAHPIGTGFFELDSRTSGESVSVTRNEDYWDKGNLAKA
CHHHHCCCHHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCC
SGITFKTVPEEATRIAELQGDVADIIYPVNPSSISEIESAENTTVKQSPSSNLTYLGFNT
CCCEEEECCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCEEECCCCCCEEEEECCC
EVEPFTDKRVRQAINMAIDKDSIINGLLDGVAEPAIGPLAPTVTGYSQNIDTFEYDQEEA
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RQLLAEAGYEDGFDATLTVNSSTASLVDLATYLQAQLEEIGIHVSVNPVESATYLEMTGD
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEECC
GETEMFIGTWGTVTVDADYGLYPMFHSSNFGAPGNRTFFANEQLDDLLEQARKETDEQKR
CCCEEEEEECEEEEEECCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHCCCHHHH
LALYEEAQNVLAEEAPVVNLYHSVLLAGMGDDIEGFWQYPASIFYLRDTEKVE
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MFKKNALWLAVAASAMLVVTACSNDPEQAENTSDEAENSASGAELTIDLSSDPVSLDPHG
CCCCCEEEEEEHHCEEEEEEECCCCHHHHCCCCHHHHCCCCCCEEEEEECCCCCCCCCCC
ANDGNSLYVMGTLYNKLVEFDENNEIQPSLATSLEQIEDDVWEAKLREDVTFHDGSAFNA
CCCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCH
EVVKANLDRVRDPDLGSPVAFLFDKITDVEVIDEYTVHIHTDGPFSPLPSHLAHPAGGMI
HHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCHHHCCCCCCCC
SKEAIDADYEAIENGEPAFSYVDAHPIGTGFFELDSRTSGESVSVTRNEDYWDKGNLAKA
CHHHHCCCHHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCC
SGITFKTVPEEATRIAELQGDVADIIYPVNPSSISEIESAENTTVKQSPSSNLTYLGFNT
CCCEEEECCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCEEECCCCCCEEEEECCC
EVEPFTDKRVRQAINMAIDKDSIINGLLDGVAEPAIGPLAPTVTGYSQNIDTFEYDQEEA
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RQLLAEAGYEDGFDATLTVNSSTASLVDLATYLQAQLEEIGIHVSVNPVESATYLEMTGD
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEECC
GETEMFIGTWGTVTVDADYGLYPMFHSSNFGAPGNRTFFANEQLDDLLEQARKETDEQKR
CCCEEEEEECEEEEEECCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHCCCHHHH
LALYEEAQNVLAEEAPVVNLYHSVLLAGMGDDIEGFWQYPASIFYLRDTEKVE
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA