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The map label for this gene is nadE [H]

Identifier: 55981869

GI number: 55981869

Start: 1785930

End: 1786775

Strand: Reverse

Name: nadE [H]

Synonym: TTHA1900

Alternate gene names: 55981869

Gene position: 1786775-1785930 (Counterclockwise)

Preceding gene: 55981870

Following gene: 55981868

Centisome position: 96.6

GC content: 67.26

Gene sequence:

>846_bases
ATGCGGATACTTGAAGCGCCCAAAGCCCAGGAGGTCCTGGAACTCAACTGGCCCCTGGTGGCGGACTTCCTCACCCGGTT
TATCCGGGAGGAGCTTTCCTGGCGGGGCTACGAGAAGGCCATCGTGGCCGTCTCCGGCGGGGTGGACTCGGCCACCACCC
TGGCCTTGGCGGTGCGGGCCTTGGGGGCGGAGAGGGTCCACGCCCTCTTCCTGCCCCACCGGGAGAGTAGCCCCACCTCC
CGGGAGCACGCCTACCTGGTGGCCGAGACCTTCGGCGTCGCCCTGGAGGAGGTGGACATCACCCCCATGGTGGAGGGCTA
CGCCGCCCTCACCCCGGACCTCACCCCCCACCGCAAGGGAAACGTCATGGCCCGGGCGCGGATGATCGTCCTCTTTGACA
AGTCCGAGGCCTACAGGGCCCTCCCCTTGGGCACGGGCAACAAGACGGAAAGGCTTTTCGGCTACTTCACCTGGCACGGG
GACGACACGCCCCCCGTGAACCCCCTGGGAGACCTCTACAAGACCCAGGTCTGGCGGCTCGCCGAGCACCTCGGGGTGCC
GGAGGAGGTGGTGAGGAAGCCCCCCACCGCCGACCTCATCCCCGGGCAGACGGACGAGGCCGATTTGGGGTTGCGCTACC
TCCGGGCCGACGTGATCCTGGAGCACTACCTGAAGGGCTATTCCGACCAGTACATCCTGGGCCTGGGCTTCACCGAGGAG
GAGCTTAGGCGGGTGAAGGAGCGGGTGAACCGCACCCACTGGAAGAGGGCCCTGCCCACGGTGGCCCTACTTTCTTCCAC
CGCCATCGGGGAGTTCTACCTGAGGCCCTTGGACTACCGGCCATGA

Upstream 100 bases:

>100_bases
CGGATCCCCCCGGTGCGCTACGACAGCCCCCTCCTCTCCGACCTCGAGGCGGGCCTGCCCCTGCTTCTTTCCGACCTGCA
AAGGGTCTTAGGGAGGCGGG

Downstream 100 bases:

>100_bases
ACGACCAGGAACTCAGAGCTTATCTGAGCCAGGCCAAGACCATCGCCGTCCTCGGGGCCCACAAGGACCCTTCCCGCCCC
GCCCACTACGTGCCCAGATA

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MRILEAPKAQEVLELNWPLVADFLTRFIREELSWRGYEKAIVAVSGGVDSATTLALAVRALGAERVHALFLPHRESSPTS
REHAYLVAETFGVALEEVDITPMVEGYAALTPDLTPHRKGNVMARARMIVLFDKSEAYRALPLGTGNKTERLFGYFTWHG
DDTPPVNPLGDLYKTQVWRLAEHLGVPEEVVRKPPTADLIPGQTDEADLGLRYLRADVILEHYLKGYSDQYILGLGFTEE
ELRRVKERVNRTHWKRALPTVALLSSTAIGEFYLRPLDYRP

Sequences:

>Translated_281_residues
MRILEAPKAQEVLELNWPLVADFLTRFIREELSWRGYEKAIVAVSGGVDSATTLALAVRALGAERVHALFLPHRESSPTS
REHAYLVAETFGVALEEVDITPMVEGYAALTPDLTPHRKGNVMARARMIVLFDKSEAYRALPLGTGNKTERLFGYFTWHG
DDTPPVNPLGDLYKTQVWRLAEHLGVPEEVVRKPPTADLIPGQTDEADLGLRYLRADVILEHYLKGYSDQYILGLGFTEE
ELRRVKERVNRTHWKRALPTVALLSSTAIGEFYLRPLDYRP
>Mature_281_residues
MRILEAPKAQEVLELNWPLVADFLTRFIREELSWRGYEKAIVAVSGGVDSATTLALAVRALGAERVHALFLPHRESSPTS
REHAYLVAETFGVALEEVDITPMVEGYAALTPDLTPHRKGNVMARARMIVLFDKSEAYRALPLGTGNKTERLFGYFTWHG
DDTPPVNPLGDLYKTQVWRLAEHLGVPEEVVRKPPTADLIPGQTDEADLGLRYLRADVILEHYLKGYSDQYILGLGFTEE
ELRRVKERVNRTHWKRALPTVALLSSTAIGEFYLRPLDYRP

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=229, Percent_Identity=26.6375545851528, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 31736; Mature: 31736

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILEAPKAQEVLELNWPLVADFLTRFIREELSWRGYEKAIVAVSGGVDSATTLALAVRA
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHH
LGAERVHALFLPHRESSPTSREHAYLVAETFGVALEEVDITPMVEGYAALTPDLTPHRKG
HCHHHEEEEEECCCCCCCCCCHHEEEEEHHHCCHHHHCCCCHHHCCHHHCCCCCCCCCCC
NVMARARMIVLFDKSEAYRALPLGTGNKTERLFGYFTWHGDDTPPVNPLGDLYKTQVWRL
CEEEEEEEEEEEECCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHH
AEHLGVPEEVVRKPPTADLIPGQTDEADLGLRYLRADVILEHYLKGYSDQYILGLGFTEE
HHHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHH
ELRRVKERVNRTHWKRALPTVALLSSTAIGEFYLRPLDYRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRILEAPKAQEVLELNWPLVADFLTRFIREELSWRGYEKAIVAVSGGVDSATTLALAVRA
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHH
LGAERVHALFLPHRESSPTSREHAYLVAETFGVALEEVDITPMVEGYAALTPDLTPHRKG
HCHHHEEEEEECCCCCCCCCCHHEEEEEHHHCCHHHHCCCCHHHCCHHHCCCCCCCCCCC
NVMARARMIVLFDKSEAYRALPLGTGNKTERLFGYFTWHGDDTPPVNPLGDLYKTQVWRL
CEEEEEEEEEEEECCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHH
AEHLGVPEEVVRKPPTADLIPGQTDEADLGLRYLRADVILEHYLKGYSDQYILGLGFTEE
HHHCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHH
ELRRVKERVNRTHWKRALPTVALLSSTAIGEFYLRPLDYRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA