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The map label for this gene is thiB [H]

Identifier: 55981777

GI number: 55981777

Start: 1686772

End: 1687806

Strand: Reverse

Name: thiB [H]

Synonym: TTHA1808

Alternate gene names: 55981777

Gene position: 1687806-1686772 (Counterclockwise)

Preceding gene: 55981778

Following gene: 55981776

Centisome position: 91.25

GC content: 70.14

Gene sequence:

>1035_bases
ATGCGGAAAATCCTGGCGGCTTTGGCGTTCCTGACCCTGGGCTTGGCCCAGGAGCTCGTGGTCCTCACCCACTCCAGCTT
CTCCCTGGACAAGGCCCTCATCGCCCGGTTCCAGGAGGAGACGGGGATCCGGCTCCGCTTCCTCAAGGGAGGCGACGCGG
GGGAGACCCTGAACAAGGCCATCCTCTCCAAGGGGGCCCCCATCGCCGACGTCCTCTACGGCTTTGACAACACCTTCCTC
TCCCGGGCCCTCGAGGCGGACATCCTCCTCCCCTACGTGAGCCCGGAGATCCGGAACCTCCGCTCCGAGCTCCTTCTGGA
CCCCAGCTTCCGGGCCATCCCCGTGGACTACGGCTTCGTCAGCCTCAACTACGACCGGGCCTACTTCCGGGGCAAGGCCC
TGCCGCAAAGGCCGGAGGACCTCGCCCGCCCGGAGTACGCCCGCCTGCTCGTGGTGGAGAACCCCGCCACCAGCTCCCCG
GGCCTCGCCTTCCTGATGGCCACGGTGGCCCGCTTCGGGGAGGACGGGTACCTGGACTTCTGGGCCAGGCTCCGGGACGG
GGGCGTCCGGGTGGCCAAGGGCTGGAGCGAGGCCTACTACACCCACTTCACCCTCCACGGCGGGGACCGGCCCCTGGTGG
TCTCCTACACCACGAGCCCCGCCGCCGAGGTCTACTACTCCGAGGGCAAGTACAAGGAGCCCCCCACCGGGAACCTCTTC
CCGGAGCTCGCCTTCTTCCAGGTGGAGTTCGTGGGCATCCTCAAGGGGACCAAGAACCTGGAGGCCGCCCGCCGCTTCGT
GGACTGGCTCCTCTCCCGCCCCGTGCAGGAGAACATCCCCACGGAGATGTGGGTCTACCCCGCCAGGCGGGACGCCCGCC
TGCCCGAGGTCTTCCGCTTCACCCCGCCTCCCGCCGGGGCCGTGCGCCTGGACCCGGCCCGCATGGCGGCGAACCGGGAG
CGGTGGATTGAGGAGTGGACCCGGGTGGTCCTCCAGGGCCAGGACCCCGAGGCGGTGCGGGCCGGGCGGCGGTGA

Upstream 100 bases:

>100_bases
GCCCCAAAGGGGCTGAGAGCATACCCTTGGAACCTGATCCGGGTCATGCCGGCGTAGGGAAGGTGACGGCCATCCCCTCC
CGGTGACAAGGAGGGGCGCG

Downstream 100 bases:

>100_bases
GCTGGGGTGGGCGCGGCCGCCCTGGTCCTCGCCTTCCTCGCCTTCGCCCTCTTCTACCCCTTAGGCCGGGTCCTGGTCCT
GGGGGTGGGGGAGGGGTTCG

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRKILAALAFLTLGLAQELVVLTHSSFSLDKALIARFQEETGIRLRFLKGGDAGETLNKAILSKGAPIADVLYGFDNTFL
SRALEADILLPYVSPEIRNLRSELLLDPSFRAIPVDYGFVSLNYDRAYFRGKALPQRPEDLARPEYARLLVVENPATSSP
GLAFLMATVARFGEDGYLDFWARLRDGGVRVAKGWSEAYYTHFTLHGGDRPLVVSYTTSPAAEVYYSEGKYKEPPTGNLF
PELAFFQVEFVGILKGTKNLEAARRFVDWLLSRPVQENIPTEMWVYPARRDARLPEVFRFTPPPAGAVRLDPARMAANRE
RWIEEWTRVVLQGQDPEAVRAGRR

Sequences:

>Translated_344_residues
MRKILAALAFLTLGLAQELVVLTHSSFSLDKALIARFQEETGIRLRFLKGGDAGETLNKAILSKGAPIADVLYGFDNTFL
SRALEADILLPYVSPEIRNLRSELLLDPSFRAIPVDYGFVSLNYDRAYFRGKALPQRPEDLARPEYARLLVVENPATSSP
GLAFLMATVARFGEDGYLDFWARLRDGGVRVAKGWSEAYYTHFTLHGGDRPLVVSYTTSPAAEVYYSEGKYKEPPTGNLF
PELAFFQVEFVGILKGTKNLEAARRFVDWLLSRPVQENIPTEMWVYPARRDARLPEVFRFTPPPAGAVRLDPARMAANRE
RWIEEWTRVVLQGQDPEAVRAGRR
>Mature_344_residues
MRKILAALAFLTLGLAQELVVLTHSSFSLDKALIARFQEETGIRLRFLKGGDAGETLNKAILSKGAPIADVLYGFDNTFL
SRALEADILLPYVSPEIRNLRSELLLDPSFRAIPVDYGFVSLNYDRAYFRGKALPQRPEDLARPEYARLLVVENPATSSP
GLAFLMATVARFGEDGYLDFWARLRDGGVRVAKGWSEAYYTHFTLHGGDRPLVVSYTTSPAAEVYYSEGKYKEPPTGNLF
PELAFFQVEFVGILKGTKNLEAARRFVDWLLSRPVQENIPTEMWVYPARRDARLPEVFRFTPPPAGAVRLDPARMAANRE
RWIEEWTRVVLQGQDPEAVRAGRR

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=317, Percent_Identity=30.5993690851735, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38761; Mature: 38761

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILAALAFLTLGLAQELVVLTHSSFSLDKALIARFQEETGIRLRFLKGGDAGETLNKA
CHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
ILSKGAPIADVLYGFDNTFLSRALEADILLPYVSPEIRNLRSELLLDPSFRAIPVDYGFV
HHHCCCCHHHHHHCCCHHHHHHHHHCCEEECCCCHHHHHHHHHHEECCCCCEEEECCCEE
SLNYDRAYFRGKALPQRPEDLARPEYARLLVVENPATSSPGLAFLMATVARFGEDGYLDF
EEECCHHHHCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHH
WARLRDGGVRVAKGWSEAYYTHFTLHGGDRPLVVSYTTSPAAEVYYSEGKYKEPPTGNLF
HHHHHCCCEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCC
PELAFFQVEFVGILKGTKNLEAARRFVDWLLSRPVQENIPTEMWVYPARRDARLPEVFRF
CHHHHHHEEEHHHHCCCCCHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCCCCCHHEEE
TPPPAGAVRLDPARMAANRERWIEEWTRVVLQGQDPEAVRAGRR
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MRKILAALAFLTLGLAQELVVLTHSSFSLDKALIARFQEETGIRLRFLKGGDAGETLNKA
CHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
ILSKGAPIADVLYGFDNTFLSRALEADILLPYVSPEIRNLRSELLLDPSFRAIPVDYGFV
HHHCCCCHHHHHHCCCHHHHHHHHHCCEEECCCCHHHHHHHHHHEECCCCCEEEECCCEE
SLNYDRAYFRGKALPQRPEDLARPEYARLLVVENPATSSPGLAFLMATVARFGEDGYLDF
EEECCHHHHCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHH
WARLRDGGVRVAKGWSEAYYTHFTLHGGDRPLVVSYTTSPAAEVYYSEGKYKEPPTGNLF
HHHHHCCCEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCC
PELAFFQVEFVGILKGTKNLEAARRFVDWLLSRPVQENIPTEMWVYPARRDARLPEVFRF
CHHHHHHEEEHHHHCCCCCHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCCCCCHHEEE
TPPPAGAVRLDPARMAANRERWIEEWTRVVLQGQDPEAVRAGRR
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]