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The map label for this gene is thiI

Identifier: 55981765

GI number: 55981765

Start: 1677039

End: 1678259

Strand: Direct

Name: thiI

Synonym: TTHA1796

Alternate gene names: 55981765

Gene position: 1677039-1678259 (Clockwise)

Preceding gene: 55981764

Following gene: 55981766

Centisome position: 90.66

GC content: 70.19

Gene sequence:

>1221_bases
ATGGAAACCCTGCTTCTCGTGCAACTCTTCCACGAGCTCGCCCTTAAGGGGAAAAACCGCCCCTTCTTCCTCAAGCGGGC
CAAGGCCCACGTGCGGCGGGCCCTAAAGGGCCTGGGGGTGGCCCTCGAGGGGGAGTGGCCCATGGCCCTCCTCTTCCGCC
TCCCCGAGGAGGCCTGGCCCGAGGCCAAGGCCCGCCTCCAGGACACCCTGGGGGTGGAACGCTTCGCCCGGGTCCATAGG
ACCCCGCCCGACCTCGAGGCCCTGAAGGCCGCCCTAGAAAAGGCCCTGGAGGGCCAGGCCTTCGGGAGCTTCCGCATCAC
CGCCAAGCGCTCCGACAAAGCGTTTCCCCTCACCTCCCCGGAGATAGAGCGGGCCCTCGGGGCTTTCGTGAAGGGAAAAA
CGGGCGCCCCGGTGCGGCTCAAGGGGGCGGAGCGGGAGTTCGTGGTCCGGGTGCTCCCCGGGGCGGCCCTCCTGGAGGTG
GAGCGCCACCCGGGCCCCGGGGGGCTTCCCCCCGGGGTCTCGGGGCGGGTGGTGGCCCTCCTCTCCGGGGGGATTGACTC
CCCGGTGGCCGCCTACCGCCTCATGCGCCGGGGGGCGGAGGTGGTCCTCGTGCACTTCCACCCCTTCCCCCTCCTCTCGG
GGGCGAGCCGGGAGAAGGCCAAGGCCCTCGCCGAGCGCCTGGCCCGCTTCCAGCACCGCCTGAGGCTCCACCTCGTCCCC
TTCAGCGAGGTCCAGCGCCACATCATCGTGGAGGCCCCCACGGCCTACCGGGTGGTCCTCTACCGCCGCTACATGCTGAG
GATCGCCGAGGCCATCGCCAGGGAAGAAGGCGCCCTCGCCCTTTGCACCGGGGACAGCCTGGGCCAGGTGGCCTCCCAGA
CCCTGGAGAACCTCCACGCCGTGAACCAGGCGGCCACCCTCCCCGTCTTCCGGCCCCTCATCGGGTGGGACAAAGAGGAG
ATCGTGGCCGAGGCCCAGAGGATCGGCACCTACGCCACCTCCATCCTCCCCGACGAGGAGTGCTGCACCCTCTTCGCCCC
CAAGCACCCCGTGACCCGGGCGAGGCTGGAGGTGGTCCTAGAGACGGAGGCCCGCCTGCCCACGGAGGAGCTTTTGGCCC
TGGCCCTAAAGGAGCGGGAAGTCCTCACCTACACCTGGCCCGGAAAGCCCCTACCCGAGGAGCCGGAAGGCGCTTTTATA
ATGGAGCATGGACCTGCTTGA

Upstream 100 bases:

>100_bases
TTCGGCCGGCCCGAGGAGCTCAAGGCGGCCGCGAGGCAAGGCTCCCTTTGCAGCCGGCTCTTCCTCGCGGTCAAGGGGCT
TTGCCCCTAGAAAGGACGTC

Downstream 100 bases:

>100_bases
GGCGAAGCGCCTTCTGGAAACGGGAAGGACCACCCCTCTAGCCCTTTTGGAAGAGGCCCTGGAGCGGGCCAAGGCCTTCC
AGGACCGGAACGCCCTCGCC

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
METLLLVQLFHELALKGKNRPFFLKRAKAHVRRALKGLGVALEGEWPMALLFRLPEEAWPEAKARLQDTLGVERFARVHR
TPPDLEALKAALEKALEGQAFGSFRITAKRSDKAFPLTSPEIERALGAFVKGKTGAPVRLKGAEREFVVRVLPGAALLEV
ERHPGPGGLPPGVSGRVVALLSGGIDSPVAAYRLMRRGAEVVLVHFHPFPLLSGASREKAKALAERLARFQHRLRLHLVP
FSEVQRHIIVEAPTAYRVVLYRRYMLRIAEAIAREEGALALCTGDSLGQVASQTLENLHAVNQAATLPVFRPLIGWDKEE
IVAEAQRIGTYATSILPDEECCTLFAPKHPVTRARLEVVLETEARLPTEELLALALKEREVLTYTWPGKPLPEEPEGAFI
MEHGPA

Sequences:

>Translated_406_residues
METLLLVQLFHELALKGKNRPFFLKRAKAHVRRALKGLGVALEGEWPMALLFRLPEEAWPEAKARLQDTLGVERFARVHR
TPPDLEALKAALEKALEGQAFGSFRITAKRSDKAFPLTSPEIERALGAFVKGKTGAPVRLKGAEREFVVRVLPGAALLEV
ERHPGPGGLPPGVSGRVVALLSGGIDSPVAAYRLMRRGAEVVLVHFHPFPLLSGASREKAKALAERLARFQHRLRLHLVP
FSEVQRHIIVEAPTAYRVVLYRRYMLRIAEAIAREEGALALCTGDSLGQVASQTLENLHAVNQAATLPVFRPLIGWDKEE
IVAEAQRIGTYATSILPDEECCTLFAPKHPVTRARLEVVLETEARLPTEELLALALKEREVLTYTWPGKPLPEEPEGAFI
MEHGPA
>Mature_406_residues
METLLLVQLFHELALKGKNRPFFLKRAKAHVRRALKGLGVALEGEWPMALLFRLPEEAWPEAKARLQDTLGVERFARVHR
TPPDLEALKAALEKALEGQAFGSFRITAKRSDKAFPLTSPEIERALGAFVKGKTGAPVRLKGAEREFVVRVLPGAALLEV
ERHPGPGGLPPGVSGRVVALLSGGIDSPVAAYRLMRRGAEVVLVHFHPFPLLSGASREKAKALAERLARFQHRLRLHLVP
FSEVQRHIIVEAPTAYRVVLYRRYMLRIAEAIAREEGALALCTGDSLGQVASQTLENLHAVNQAATLPVFRPLIGWDKEE
IVAEAQRIGTYATSILPDEECCTLFAPKHPVTRARLEVVLETEARLPTEELLALALKEREVLTYTWPGKPLPEEPEGAFI
MEHGPA

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=364, Percent_Identity=30.2197802197802, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_THET2 (Q72HP1)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005414.1
- HSSP:   Q81KU0
- ProteinModelPortal:   Q72HP1
- SMR:   Q72HP1
- STRING:   Q72HP1
- GeneID:   2775901
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0301
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   TTHE262724:TT_C1445-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 44875; Mature: 44875

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS51165 THUMP

Important sites: BINDING 262-262 BINDING 284-284 BINDING 293-293

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLLLVQLFHELALKGKNRPFFLKRAKAHVRRALKGLGVALEGEWPMALLFRLPEEAWP
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCHHHCH
EAKARLQDTLGVERFARVHRTPPDLEALKAALEKALEGQAFGSFRITAKRSDKAFPLTSP
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCH
EIERALGAFVKGKTGAPVRLKGAEREFVVRVLPGAALLEVERHPGPGGLPPGVSGRVVAL
HHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEE
LSGGIDSPVAAYRLMRRGAEVVLVHFHPFPLLSGASREKAKALAERLARFQHRLRLHLVP
EECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECC
FSEVQRHIIVEAPTAYRVVLYRRYMLRIAEAIAREEGALALCTGDSLGQVASQTLENLHA
HHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
VNQAATLPVFRPLIGWDKEEIVAEAQRIGTYATSILPDEECCTLFAPKHPVTRARLEVVL
HHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCCHHHHHHEEE
ETEARLPTEELLALALKEREVLTYTWPGKPLPEEPEGAFIMEHGPA
ECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEECCCCC
>Mature Secondary Structure
METLLLVQLFHELALKGKNRPFFLKRAKAHVRRALKGLGVALEGEWPMALLFRLPEEAWP
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCHHHCH
EAKARLQDTLGVERFARVHRTPPDLEALKAALEKALEGQAFGSFRITAKRSDKAFPLTSP
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCH
EIERALGAFVKGKTGAPVRLKGAEREFVVRVLPGAALLEVERHPGPGGLPPGVSGRVVAL
HHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEE
LSGGIDSPVAAYRLMRRGAEVVLVHFHPFPLLSGASREKAKALAERLARFQHRLRLHLVP
EECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECC
FSEVQRHIIVEAPTAYRVVLYRRYMLRIAEAIAREEGALALCTGDSLGQVASQTLENLHA
HHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
VNQAATLPVFRPLIGWDKEEIVAEAQRIGTYATSILPDEECCTLFAPKHPVTRARLEVVL
HHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCCHHHHHHEEE
ETEARLPTEELLALALKEREVLTYTWPGKPLPEEPEGAFIMEHGPA
ECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA