Click here to switch to the map view.

The map label for this gene is pilT [H]

Identifier: 55981743

GI number: 55981743

Start: 1661957

End: 1663063

Strand: Reverse

Name: pilT [H]

Synonym: TTHA1774

Alternate gene names: 55981743

Gene position: 1663063-1661957 (Counterclockwise)

Preceding gene: 55981744

Following gene: 55981742

Centisome position: 89.91

GC content: 67.12

Gene sequence:

>1107_bases
ATGAGCGAGGCCCAAGGCCAGGGCAAGCAGAGCCTCGTGGAGATGCTGAAGGCCATGGTCCAGGCCCGGGCCTCGGACAT
CCACCTGCAGGCCGGGGCCCCGCCCACCGTCCGGATAGACGGCAAGCTCCGCCCCTTCGGCAACCGCCCCCTGACCCCCA
AGGAGGTGGAGGCCATCGCCCGGGCCCTCCTCACCCCGGAACAGCTGGAGGAGCTGGAGTACCGCAAGGAGATGGACTTC
GCCTACACCATCCCCGGGGTGGCCCGCTTCCGCTGCAACCTCCTGAGGCAACGGGGGAGCTTCGGCCTGGTGATGCGGGT
GGTCTCCGAGGTGATCCCGAGCTTTGAGGCCCTGGGCCTCCCCCGGGAGGTGATGGAAAGCCTCGCCGCCAAGGAGCGGG
GCCTCATCCTGGTCACGGGGCCCACGGGGAGCGGCAAGAGCACCACCCTGGCGGCCCTCATTGACCACATCAACCTTCAC
TACGCCAAGAACATCATCACCATTGAGGACCCCATTGAGTTCCTGCACAAGCACAAGAAGAGCCTCGTGGTCCAGCGGGA
GGTGGGGCTGGACACGGACAGCTTCTACACCGGCCTCAAGTACGCCCTCCGCCAGGACCCCGACGTGATCATGGTGGGGG
AGATGCGGGACCGGGAGACGGTGGAGGCCGCCCTCATGGCGGCGCAGACGGGCCACCTGGTCCTCTCCACCCTCCACACC
CTGGACGCCTGGCGGACCATCAACCGGATCATTGACTTCTTTCCCCTGCACGAGCACCGACAGGTGCGCGTCCTCCTCGC
CGAGTCCCTCCTCGGCATCCTCTCCCAGCGCCTCCTCCCCAAGGCCGACGGCCAGGGCCGGGTCCTCGCCCTGGAGATCC
TCATCGCTACCCCCTACGTGCGGGAGCTTCTCAAGGACGAGGAGAAGATGCCCCAGATCAAGGAGGCCATGATGGAGGGG
TCCCTCTACGGGATGCGCACCTTTGACCAGCACCTGGTGGAGCTCTACACCGAGGGGCTCATCTCCCTGGAGGACGCCCT
CTCCGCCGCCACCAGCCCCCACGAGTTCCGGCTCCTCCTCACCAAGGCCACGGGGCAGACCTACTGA

Upstream 100 bases:

>100_bases
AGACCCTCCTGCCCCTCTCCGACTGGGTCGCGGGGGCCAGGGGGATCGTGGCCGGGAGGTTCCCCGAGGCGCGGCTTATA
GACAACCTGGAGGTCTACCC

Downstream 100 bases:

>100_bases
CGCCCTTTCCCTCGCCCGCCTGGCCCCGAAGAAGCCCCGTGGGAACCCTTTTCCGGGCCCCCAGGGGGTGGGGCCGTTCC
ACGCTTCCAGAGGGGGTATA

Product: pili retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSEAQGQGKQSLVEMLKAMVQARASDIHLQAGAPPTVRIDGKLRPFGNRPLTPKEVEAIARALLTPEQLEELEYRKEMDF
AYTIPGVARFRCNLLRQRGSFGLVMRVVSEVIPSFEALGLPREVMESLAAKERGLILVTGPTGSGKSTTLAALIDHINLH
YAKNIITIEDPIEFLHKHKKSLVVQREVGLDTDSFYTGLKYALRQDPDVIMVGEMRDRETVEAALMAAQTGHLVLSTLHT
LDAWRTINRIIDFFPLHEHRQVRVLLAESLLGILSQRLLPKADGQGRVLALEILIATPYVRELLKDEEKMPQIKEAMMEG
SLYGMRTFDQHLVELYTEGLISLEDALSAATSPHEFRLLLTKATGQTY

Sequences:

>Translated_368_residues
MSEAQGQGKQSLVEMLKAMVQARASDIHLQAGAPPTVRIDGKLRPFGNRPLTPKEVEAIARALLTPEQLEELEYRKEMDF
AYTIPGVARFRCNLLRQRGSFGLVMRVVSEVIPSFEALGLPREVMESLAAKERGLILVTGPTGSGKSTTLAALIDHINLH
YAKNIITIEDPIEFLHKHKKSLVVQREVGLDTDSFYTGLKYALRQDPDVIMVGEMRDRETVEAALMAAQTGHLVLSTLHT
LDAWRTINRIIDFFPLHEHRQVRVLLAESLLGILSQRLLPKADGQGRVLALEILIATPYVRELLKDEEKMPQIKEAMMEG
SLYGMRTFDQHLVELYTEGLISLEDALSAATSPHEFRLLLTKATGQTY
>Mature_367_residues
SEAQGQGKQSLVEMLKAMVQARASDIHLQAGAPPTVRIDGKLRPFGNRPLTPKEVEAIARALLTPEQLEELEYRKEMDFA
YTIPGVARFRCNLLRQRGSFGLVMRVVSEVIPSFEALGLPREVMESLAAKERGLILVTGPTGSGKSTTLAALIDHINLHY
AKNIITIEDPIEFLHKHKKSLVVQREVGLDTDSFYTGLKYALRQDPDVIMVGEMRDRETVEAALMAAQTGHLVLSTLHTL
DAWRTINRIIDFFPLHEHRQVRVLLAESLLGILSQRLLPKADGQGRVLALEILIATPYVRELLKDEEKMPQIKEAMMEGS
LYGMRTFDQHLVELYTEGLISLEDALSAATSPHEFRLLLTKATGQTY

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=330, Percent_Identity=41.5151515151515, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1786296, Length=390, Percent_Identity=30.5128205128205, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1789723, Length=293, Percent_Identity=35.4948805460751, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41171; Mature: 41040

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAQGQGKQSLVEMLKAMVQARASDIHLQAGAPPTVRIDGKLRPFGNRPLTPKEVEAIA
CCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCEECCCCCCCCCHHHHHHHH
RALLTPEQLEELEYRKEMDFAYTIPGVARFRCNLLRQRGSFGLVMRVVSEVIPSFEALGL
HHHCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCC
PREVMESLAAKERGLILVTGPTGSGKSTTLAALIDHINLHYAKNIITIEDPIEFLHKHKK
CHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHH
SLVVQREVGLDTDSFYTGLKYALRQDPDVIMVGEMRDRETVEAALMAAQTGHLVLSTLHT
HHEEHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHH
LDAWRTINRIIDFFPLHEHRQVRVLLAESLLGILSQRLLPKADGQGRVLALEILIATPYV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHCHHH
RELLKDEEKMPQIKEAMMEGSLYGMRTFDQHLVELYTEGLISLEDALSAATSPHEFRLLL
HHHHHCHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
TKATGQTY
EECCCCCC
>Mature Secondary Structure 
SEAQGQGKQSLVEMLKAMVQARASDIHLQAGAPPTVRIDGKLRPFGNRPLTPKEVEAIA
CCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCEECCCCCCCCCHHHHHHHH
RALLTPEQLEELEYRKEMDFAYTIPGVARFRCNLLRQRGSFGLVMRVVSEVIPSFEALGL
HHHCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCC
PREVMESLAAKERGLILVTGPTGSGKSTTLAALIDHINLHYAKNIITIEDPIEFLHKHKK
CHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHH
SLVVQREVGLDTDSFYTGLKYALRQDPDVIMVGEMRDRETVEAALMAAQTGHLVLSTLHT
HHEEHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHH
LDAWRTINRIIDFFPLHEHRQVRVLLAESLLGILSQRLLPKADGQGRVLALEILIATPYV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHCHHH
RELLKDEEKMPQIKEAMMEGSLYGMRTFDQHLVELYTEGLISLEDALSAATSPHEFRLLL
HHHHHCHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
TKATGQTY
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]