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The map label for this gene is afsQ2 [H]

Identifier: 55981561

GI number: 55981561

Start: 1514022

End: 1515326

Strand: Reverse

Name: afsQ2 [H]

Synonym: TTHA1592

Alternate gene names: 55981561

Gene position: 1515326-1514022 (Counterclockwise)

Preceding gene: 55981562

Following gene: 55981560

Centisome position: 81.92

GC content: 73.87

Gene sequence:

>1305_bases
ATGGCTACCGCCTTTTCCTCCCTGAGGGCTAGGCTCTTCGCCCTCCTCCTCCTCGCCCTTCTCCTCCTCCTCGCCCCCTT
GGCCTGGCTTTCCGCCCGGGAGGCGGAGCGGGCCGCGAGCGAGGACCTGAGGCGGGCCCTCTACACCCGGCTTTACCTCC
TCCAGGCGGAGGGGGTCCAGGACGAGGAGCGGCTCCTCCTGGAGCTTTTCCGCCTGGCCCAGCTCTTCGGGGGCGGGGTG
GGGTTTGTCCTCCGGGAGGGGGAGGCCCGCTTCACCCAGCTCGGGGACTGGCCTCTGCCCCCCGGGCTTCCCGCGGCTTT
GGCGGCGGAAGGGGTCTACCAGGGGGTCTTTCGGGGGACGCTCTATGTGGCCCTGGCCCAAGGGGGGCTCGCCTACGGCC
TTGCGGTGCCCCTGGAAGGGGTTTCCGGGCTCGGCCGGCGCCTCCTCGCCCGCTACGCCCTCTGGGGCGGGGGGCTTTTG
CTTGGGGTCTTCCTCCTCGCCGCCCTCGCCCTCTCCTGGACCCTCCGTCCCCTCCGGGACCTGGAGAAGGCCTTGCGGGC
CCGGGCGCCCGAGGACCTGAGCCCCCTCCCCGACCCGGGCCTGGCCGAGCTAAGGCCGGTGGTGGCGGCGCTGAACGCCC
TTTTCGCCCGGGTGGGGAGGCTCCTCGCCGAGCTCGCCGAGAAGGAGGAGGCGGCCCGGCGCTTCGCCCGCCACGCCTCC
CACGAGCTCCGCACCCCCCTCACCGCCCTCAAGGGCTACCTGGAGGTGCTCCGCCGGAAAAACGAGCCCCGGGCCCTGGA
GGGGGCCTTGCGGGAGGCCGGGCGCATGGAGGCCCTCCTCGCGGGGCTTCTCCGCCTCTCCCGCCTCGAGGCCCTTCCGC
CCCAGAGGGTCCCCTTGGACCTCCGGGCCTTCCTGGAGGCCCAGGGCCTCGAGGTCCAGGGGGAGGGGAGGGTCCTGGCC
GACCCCGAGCTTTTGGCCCTCGCGGTGGAGAACGTTCTGGAGAACGCCCGCCGCCACGGCAGGCTTCCCGTGCGGGCGGA
GCTTTCCCGGGAAGGGGAAGGGGTCTGGGTCTGGCTCGTGGACGCGGGCCCCGGGTTCCCCGAGGACCTCCTTCCCCGGG
TCCTGGAGCCCTTCGTCCACGGGGGCAAGGGGACGGGGCTCGGCCTCGCCCTGGTGGCCGCGGTGGCCCGGGCCCACGGG
GGCAGGGTCCGCGTGGAGAACCGCGGGGGGGCGGGGGTGGGGCTTTACCTGCCTTTGGCTCCGCTTAAGGTTTCCCCCGG
TGTGCCCTTTGGGGAGGGGGGCTAG

Upstream 100 bases:

>100_bases
GTCTGGGGGGAGGAGGTGGAGCCCGCCACCTTGGAGGTCCACCTCTCCGCCCTGAGAAAGGCCCTGGGGGAGCCCAGTCC
CATCCAGACGGTGCGCGGGT

Downstream 100 bases:

>100_bases
CCTGGGTCCTGGGAGGTGATGCGGGATGAAGAAAGCGGTTGTCGGCGTGCTGGCTCTTTTGGCGGGCCTCTCCCTGGCCC
AGCAGGTGGACTACCGGGAG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MATAFSSLRARLFALLLLALLLLLAPLAWLSAREAERAASEDLRRALYTRLYLLQAEGVQDEERLLLELFRLAQLFGGGV
GFVLREGEARFTQLGDWPLPPGLPAALAAEGVYQGVFRGTLYVALAQGGLAYGLAVPLEGVSGLGRRLLARYALWGGGLL
LGVFLLAALALSWTLRPLRDLEKALRARAPEDLSPLPDPGLAELRPVVAALNALFARVGRLLAELAEKEEAARRFARHAS
HELRTPLTALKGYLEVLRRKNEPRALEGALREAGRMEALLAGLLRLSRLEALPPQRVPLDLRAFLEAQGLEVQGEGRVLA
DPELLALAVENVLENARRHGRLPVRAELSREGEGVWVWLVDAGPGFPEDLLPRVLEPFVHGGKGTGLGLALVAAVARAHG
GRVRVENRGGAGVGLYLPLAPLKVSPGVPFGEGG

Sequences:

>Translated_434_residues
MATAFSSLRARLFALLLLALLLLLAPLAWLSAREAERAASEDLRRALYTRLYLLQAEGVQDEERLLLELFRLAQLFGGGV
GFVLREGEARFTQLGDWPLPPGLPAALAAEGVYQGVFRGTLYVALAQGGLAYGLAVPLEGVSGLGRRLLARYALWGGGLL
LGVFLLAALALSWTLRPLRDLEKALRARAPEDLSPLPDPGLAELRPVVAALNALFARVGRLLAELAEKEEAARRFARHAS
HELRTPLTALKGYLEVLRRKNEPRALEGALREAGRMEALLAGLLRLSRLEALPPQRVPLDLRAFLEAQGLEVQGEGRVLA
DPELLALAVENVLENARRHGRLPVRAELSREGEGVWVWLVDAGPGFPEDLLPRVLEPFVHGGKGTGLGLALVAAVARAHG
GRVRVENRGGAGVGLYLPLAPLKVSPGVPFGEGG
>Mature_433_residues
ATAFSSLRARLFALLLLALLLLLAPLAWLSAREAERAASEDLRRALYTRLYLLQAEGVQDEERLLLELFRLAQLFGGGVG
FVLREGEARFTQLGDWPLPPGLPAALAAEGVYQGVFRGTLYVALAQGGLAYGLAVPLEGVSGLGRRLLARYALWGGGLLL
GVFLLAALALSWTLRPLRDLEKALRARAPEDLSPLPDPGLAELRPVVAALNALFARVGRLLAELAEKEEAARRFARHASH
ELRTPLTALKGYLEVLRRKNEPRALEGALREAGRMEALLAGLLRLSRLEALPPQRVPLDLRAFLEAQGLEVQGEGRVLAD
PELLALAVENVLENARRHGRLPVRAELSREGEGVWVWLVDAGPGFPEDLLPRVLEPFVHGGKGTGLGLALVAAVARAHGG
RVRVENRGGAGVGLYLPLAPLKVSPGVPFGEGG

Specific function: Forms part of a two-component regulatory system AfsQ1/AfsQ2 involved in secondary metabolism. May activate AfsQ1 by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790861, Length=292, Percent_Identity=30.4794520547945, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1786600, Length=239, Percent_Identity=34.3096234309623, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789403, Length=267, Percent_Identity=32.5842696629214, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=27.1062271062271, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788393, Length=242, Percent_Identity=30.1652892561983, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=27.5, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786783, Length=322, Percent_Identity=25.776397515528, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=28.6919831223629, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789808, Length=264, Percent_Identity=27.6515151515151, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787894, Length=235, Percent_Identity=29.3617021276596, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46685; Mature: 46554

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAFSSLRARLFALLLLALLLLLAPLAWLSAREAERAASEDLRRALYTRLYLLQAEGVQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEERLLLELFRLAQLFGGGVGFVLREGEARFTQLGDWPLPPGLPAALAAEGVYQGVFRGT
HHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LYVALAQGGLAYGLAVPLEGVSGLGRRLLARYALWGGGLLLGVFLLAALALSWTLRPLRD
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LEKALRARAPEDLSPLPDPGLAELRPVVAALNALFARVGRLLAELAEKEEAARRFARHAS
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HELRTPLTALKGYLEVLRRKNEPRALEGALREAGRMEALLAGLLRLSRLEALPPQRVPLD
HHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
LRAFLEAQGLEVQGEGRVLADPELLALAVENVLENARRHGRLPVRAELSREGEGVWVWLV
HHHHHHHCCCEECCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEEE
DAGPGFPEDLLPRVLEPFVHGGKGTGLGLALVAAVARAHGGRVRVENRGGAGVGLYLPLA
ECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCC
PLKVSPGVPFGEGG
CEEECCCCCCCCCC
>Mature Secondary Structure 
ATAFSSLRARLFALLLLALLLLLAPLAWLSAREAERAASEDLRRALYTRLYLLQAEGVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEERLLLELFRLAQLFGGGVGFVLREGEARFTQLGDWPLPPGLPAALAAEGVYQGVFRGT
HHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LYVALAQGGLAYGLAVPLEGVSGLGRRLLARYALWGGGLLLGVFLLAALALSWTLRPLRD
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LEKALRARAPEDLSPLPDPGLAELRPVVAALNALFARVGRLLAELAEKEEAARRFARHAS
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HELRTPLTALKGYLEVLRRKNEPRALEGALREAGRMEALLAGLLRLSRLEALPPQRVPLD
HHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
LRAFLEAQGLEVQGEGRVLADPELLALAVENVLENARRHGRLPVRAELSREGEGVWVWLV
HHHHHHHCCCEECCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEEE
DAGPGFPEDLLPRVLEPFVHGGKGTGLGLALVAAVARAHGGRVRVENRGGAGVGLYLPLA
ECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCC
PLKVSPGVPFGEGG
CEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1339426; 12000953 [H]