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The map label for this gene is tilS [H]

Identifier: 55981511

GI number: 55981511

Start: 1469424

End: 1470947

Strand: Direct

Name: tilS [H]

Synonym: TTHA1542

Alternate gene names: 55981511

Gene position: 1469424-1470947 (Clockwise)

Preceding gene: 55981510

Following gene: 55981515

Centisome position: 79.44

GC content: 74.34

Gene sequence:

>1524_bases
ATGGATGTCCCGGCCTTCCGCGAGCGCCTTCTTCGCCTCGCCCCCAAAGACCCCCTCGTCCTCGCCGTCTCCGGGGGCGG
GGACTCCGTGGCCCTGGCCCTCCTGGTGAAGGAAGCAGGGCGCCAGGCGGTGGTGGCCCACCTGGACCACGGCCTGCGCC
CGGAAAGCCCCCTGGACCAGGCCTTCGTCCGGGCCCTGGCGGAAAGGCTGGGCTTCCCCTTCTTCACCGAGCGGGTGGAG
GTGGCCCGGATCGCCCAGGCCCGGGGCGAGAACCTGGAGGCGGTGGCCCGGGAAGTGCGCTACGCCTTCCTCCACCGGGT
GGCCCGGGAGGTGGGGGCCCGGGCGATCCTCACCGCCCACACCCTGGACGACCAGGCGGAGACCGTGCTCCTCCAGCTCC
TCCAGGGGACCGCCCGGGCCACGGGCATCCGGGAGCGGGAGGGGATCGTGGTCCGCCCCCTTTTGGCCCACACCCGGGAG
GAGCTTCGGGCCTACCTCCGGGCCCGAGGAGAGGCCTGGCGCGAGGACCCCACGAACCAGGACCCCGCCCTGGACCGGAA
CTTCCTCCGGCTCTTCGTCTTTCCCCTCCTGGAGGAGCGCTTTCCCGCGGCCAAGCGCGCCCTGGCCCGCTTCGCCGAGG
CCCGGGCCGAGGAGGAAGGGGTCCTGGAGCGCCAGGCCGAGGCCCGGCTCCTCCCCGACCCCCGCTTCTTCGTCCCCGCC
TTCCGGGCGGCGCCCCTCCTCGAGGCTCCCCTGGCGGTGCGCCGCCGGGCCTTGAGGCGGCTTCTGGAAAAGCTCGGCCT
TAGGCCGGAGGCCCGCCTCGTCCTCCTCCTGGAGGAGGCCCTCCGCGGCCGGCCCCAGACCCTCCCGGGGGGCGTCCTGG
CCCGGCGCAAGGGGGGCACCCTCTTCCTCCTCCCCCCAAGGCCCCGCCTGCCCCTGCCCCCGGGGTTCCGCCGCCCCGCG
CCCGGGGACTACCTGGAGCGGCCTTCCGGGAGGAAGCGCCTCGTGGACTTCCTGGCGGAAAAAGGAGTCCCCCGGGAGCT
CAAGCCCCTCTGGCCCGTGCGCGCCGAGGGCAACCGGGTGGTGGAGGTCCTGGGCCTTTACCCGCCCCCTCCCGAGGAGG
CCCACATGGCGGAGGCCTTGGCGGAGGCGGCCTCGGCCTTCCGGGAAGGGGAGGTGCCGGTGGGGGCGGTCCTCGTCCTC
CCGGGCCGCGTCCTCCGGGCCCACAACCGGGTGGAGGGGCTAAGGGACCCCACGGCCCACGCGGAGATGCTCCTCCTTAG
GGAGGCGGGGCCCGAGGCCCGGGGCGGGCGGCTTTACGTGACCCTCGAGCCCTGCCTCATGTGCCACCACGCCCTGGCCC
AGGCGGGGGTGGAAGTGGTCTACGGGGCGGAAAACCTCAAGGAAGGCGCCCTCACCCGCTTCGGCCTCCCCACCCGGGCC
CGGGGTGGTGTGCGGGAAAGGGAGTGTGCTAAGCTCTTAAGGGACTTCTTCGCCCGGCTCAGGGAGGGGTGCCGGAGCGG
TTGA

Upstream 100 bases:

>100_bases
AGGCGATGCCTCATAATCGTGGTGTCGTGGGTTCGAGTCCCACCGCCGCCACCAGGAGGCCCCCCGGGAAACCGGGGGGC
CGTGGCATCATGGAAGGCGC

Downstream 100 bases:

>100_bases
ACGGGCCGGTCTCGAAAACCGGTAGGCCCCTCGCGGGGCCTCGCGGGTTCGAATCCCGCCCCCTCCGCCAAAAGGGATGG
AGGCGGCTTCTGGCCGCCTC

Product: cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MDVPAFRERLLRLAPKDPLVLAVSGGGDSVALALLVKEAGRQAVVAHLDHGLRPESPLDQAFVRALAERLGFPFFTERVE
VARIAQARGENLEAVAREVRYAFLHRVAREVGARAILTAHTLDDQAETVLLQLLQGTARATGIREREGIVVRPLLAHTRE
ELRAYLRARGEAWREDPTNQDPALDRNFLRLFVFPLLEERFPAAKRALARFAEARAEEEGVLERQAEARLLPDPRFFVPA
FRAAPLLEAPLAVRRRALRRLLEKLGLRPEARLVLLLEEALRGRPQTLPGGVLARRKGGTLFLLPPRPRLPLPPGFRRPA
PGDYLERPSGRKRLVDFLAEKGVPRELKPLWPVRAEGNRVVEVLGLYPPPPEEAHMAEALAEAASAFREGEVPVGAVLVL
PGRVLRAHNRVEGLRDPTAHAEMLLLREAGPEARGGRLYVTLEPCLMCHHALAQAGVEVVYGAENLKEGALTRFGLPTRA
RGGVRERECAKLLRDFFARLREGCRSG

Sequences:

>Translated_507_residues
MDVPAFRERLLRLAPKDPLVLAVSGGGDSVALALLVKEAGRQAVVAHLDHGLRPESPLDQAFVRALAERLGFPFFTERVE
VARIAQARGENLEAVAREVRYAFLHRVAREVGARAILTAHTLDDQAETVLLQLLQGTARATGIREREGIVVRPLLAHTRE
ELRAYLRARGEAWREDPTNQDPALDRNFLRLFVFPLLEERFPAAKRALARFAEARAEEEGVLERQAEARLLPDPRFFVPA
FRAAPLLEAPLAVRRRALRRLLEKLGLRPEARLVLLLEEALRGRPQTLPGGVLARRKGGTLFLLPPRPRLPLPPGFRRPA
PGDYLERPSGRKRLVDFLAEKGVPRELKPLWPVRAEGNRVVEVLGLYPPPPEEAHMAEALAEAASAFREGEVPVGAVLVL
PGRVLRAHNRVEGLRDPTAHAEMLLLREAGPEARGGRLYVTLEPCLMCHHALAQAGVEVVYGAENLKEGALTRFGLPTRA
RGGVRERECAKLLRDFFARLREGCRSG
>Mature_507_residues
MDVPAFRERLLRLAPKDPLVLAVSGGGDSVALALLVKEAGRQAVVAHLDHGLRPESPLDQAFVRALAERLGFPFFTERVE
VARIAQARGENLEAVAREVRYAFLHRVAREVGARAILTAHTLDDQAETVLLQLLQGTARATGIREREGIVVRPLLAHTRE
ELRAYLRARGEAWREDPTNQDPALDRNFLRLFVFPLLEERFPAAKRALARFAEARAEEEGVLERQAEARLLPDPRFFVPA
FRAAPLLEAPLAVRRRALRRLLEKLGLRPEARLVLLLEEALRGRPQTLPGGVLARRKGGTLFLLPPRPRLPLPPGFRRPA
PGDYLERPSGRKRLVDFLAEKGVPRELKPLWPVRAEGNRVVEVLGLYPPPPEEAHMAEALAEAASAFREGEVPVGAVLVL
PGRVLRAHNRVEGLRDPTAHAEMLLLREAGPEARGGRLYVTLEPCLMCHHALAQAGVEVVYGAENLKEGALTRFGLPTRA
RGGVRERECAKLLRDFFARLREGCRSG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CMP/dCMP deaminase zinc-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=213, Percent_Identity=37.5586854460094, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693172, Length=143, Percent_Identity=43.3566433566434, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17541138, Length=148, Percent_Identity=35.1351351351351, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355527, Length=143, Percent_Identity=39.1608391608392, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016192
- InterPro:   IPR002125
- InterPro:   IPR016193
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00383 dCMP_cyt_deam_1 [H]

EC number: NA

Molecular weight: Translated: 56309; Mature: 56309

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVPAFRERLLRLAPKDPLVLAVSGGGDSVALALLVKEAGRQAVVAHLDHGLRPESPLDQ
CCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHH
AFVRALAERLGFPFFTERVEVARIAQARGENLEAVAREVRYAFLHRVAREVGARAILTAH
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
TLDDQAETVLLQLLQGTARATGIREREGIVVRPLLAHTREELRAYLRARGEAWREDPTNQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DPALDRNFLRLFVFPLLEERFPAAKRALARFAEARAEEEGVLERQAEARLLPDPRFFVPA
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
FRAAPLLEAPLAVRRRALRRLLEKLGLRPEARLVLLLEEALRGRPQTLPGGVLARRKGGT
HHCCHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHEECCCCE
LFLLPPRPRLPLPPGFRRPAPGDYLERPSGRKRLVDFLAEKGVPRELKPLWPVRAEGNRV
EEEECCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
VEVLGLYPPPPEEAHMAEALAEAASAFREGEVPVGAVLVLPGRVLRAHNRVEGLRDPTAH
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHH
AEMLLLREAGPEARGGRLYVTLEPCLMCHHALAQAGVEVVYGAENLKEGALTRFGLPTRA
HHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHCCCEEEECCHHHHHCCHHHCCCCCCC
RGGVRERECAKLLRDFFARLREGCRSG
CCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVPAFRERLLRLAPKDPLVLAVSGGGDSVALALLVKEAGRQAVVAHLDHGLRPESPLDQ
CCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHH
AFVRALAERLGFPFFTERVEVARIAQARGENLEAVAREVRYAFLHRVAREVGARAILTAH
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
TLDDQAETVLLQLLQGTARATGIREREGIVVRPLLAHTREELRAYLRARGEAWREDPTNQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DPALDRNFLRLFVFPLLEERFPAAKRALARFAEARAEEEGVLERQAEARLLPDPRFFVPA
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
FRAAPLLEAPLAVRRRALRRLLEKLGLRPEARLVLLLEEALRGRPQTLPGGVLARRKGGT
HHCCHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHEECCCCE
LFLLPPRPRLPLPPGFRRPAPGDYLERPSGRKRLVDFLAEKGVPRELKPLWPVRAEGNRV
EEEECCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
VEVLGLYPPPPEEAHMAEALAEAASAFREGEVPVGAVLVLPGRVLRAHNRVEGLRDPTAH
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHH
AEMLLLREAGPEARGGRLYVTLEPCLMCHHALAQAGVEVVYGAENLKEGALTRFGLPTRA
HHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHCCCEEEECCHHHHHCCHHHCCCCCCC
RGGVRERECAKLLRDFFARLREGCRSG
CCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA