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The map label for this gene is rlmI [H]

Identifier: 55981462

GI number: 55981462

Start: 1421065

End: 1422222

Strand: Reverse

Name: rlmI [H]

Synonym: TTHA1493

Alternate gene names: 55981462

Gene position: 1422222-1421065 (Counterclockwise)

Preceding gene: 55981465

Following gene: 55981460

Centisome position: 76.89

GC content: 71.33

Gene sequence:

>1158_bases
ATGCTGGGGCCTGTGCTGAGGCTTGTGGTCAAGGCGGGCAAGGAGCGGAAGCTCCGCAACTTCTACCCGAACCTCTACCG
GGACGAGATCGCCGCCCCGCCCGAGGGGGTGGGGGTGGCGGAGGCCGTGGACGCGGAGGGGCACTTTTTGGCCGTGGGCT
ACTACGACCCCCGGAGCCGGGTGCCCTTTCGGGCCTTCCGCTTTGACCCGGGGCCCCTGAACCGCGCCTTCTTCCAGGGG
CGGTTCGCCCGGGCGCTCCGGCGCAGGCAAGGCCTGGGGGAGAGCCACCGCCTTGTCCACGGGGAGGCGGACGGGCTTCC
CGGCCTCGTGGTGGACCGCTTTGGGGAGGTCCTGGTCCTCCAGGTCCGCTCCCGGGGCATGGAGGCCCTGCGGGAGGTGT
GGCTTCCCGCCCTCTTGGAGGTGGTGGCCCCCAAAGGGGTCTACGAGCGGAGCGACGTGGAGGCCCGGCGGCAGGAGGGC
CTTCCCGAAAGGGTGGGGGTGGTTTACGGGGAGGTGCCGGAGGTCCTCGAGGTGGAGGAGGACGGCCTCCGCTTCCCCAT
CCCCCTCGCCCTCGCCCAGAAGACGGGGTACTACCTGGACCAGCGGGAGAACCGCCGCCTCTTTGAGGCCATGGTGCGTC
CCGGGGAGCGGGTCCTGGACGTGTACAGCTACGTGGGGGGGTTCGCCCTGAGGGCGGCCCGCAAGGGGGCGTACGCCCTC
GCGGTGGACAAGGACCTCGAGGCCTTGGGTGTCCTGGACCAGGCGGCCCTGCGCCTGGGCCTCCGGGTGGACATCCGCCA
CGGGGAGGCCCTCCCCACCCTCCGGGGCCTGGAGGGGCCCTTCCACCACGTCCTCCTGGACCCCCCCACCCTGGTGAAGC
GCCCGGAGGAGCTTCCCGCCATGAAGCGCCACCTGGTGGACCTCGTCCGGGAAGCCTTGCGGCTCCTCGCGGAGGAGGGG
TTTTTGTGGCTTTCCTCCTGTAGCTACCACCTCCGCCTGGAGGACCTCTTGGAGGTGGCCCGCCGGGCCGCCGCCGACCT
CGGCCGCAGGCTCCGGGTCCACCGGGTGACCTACCAGCCCGAGGACCACCCCTGGAGCCTCCACATCCCTGAAAGCCTCT
ACCTCAAGACCCTCGTCCTCCAGGACGACCCCCTCTGA

Upstream 100 bases:

>100_bases
GCCGTGAGCCGGCCCAGCCCCACCACCAGAAGGGCCACGCCGAGGAAGAGGGCGAGGGCGAGCCATCGCTTCCGGTTCAT
GGCTCCACTATAGCAGGCGT

Downstream 100 bases:

>100_bases
TACCGCCCCACGCGGGCGCAAGCCCGCGTGGGGCCTTCCCGTGGCCCTCGCCGGGGCCTCAGGCCCCGCCCAGGGCGCCC
CCCGGCTTCACCTTGGGCAC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MLGPVLRLVVKAGKERKLRNFYPNLYRDEIAAPPEGVGVAEAVDAEGHFLAVGYYDPRSRVPFRAFRFDPGPLNRAFFQG
RFARALRRRQGLGESHRLVHGEADGLPGLVVDRFGEVLVLQVRSRGMEALREVWLPALLEVVAPKGVYERSDVEARRQEG
LPERVGVVYGEVPEVLEVEEDGLRFPIPLALAQKTGYYLDQRENRRLFEAMVRPGERVLDVYSYVGGFALRAARKGAYAL
AVDKDLEALGVLDQAALRLGLRVDIRHGEALPTLRGLEGPFHHVLLDPPTLVKRPEELPAMKRHLVDLVREALRLLAEEG
FLWLSSCSYHLRLEDLLEVARRAAADLGRRLRVHRVTYQPEDHPWSLHIPESLYLKTLVLQDDPL

Sequences:

>Translated_385_residues
MLGPVLRLVVKAGKERKLRNFYPNLYRDEIAAPPEGVGVAEAVDAEGHFLAVGYYDPRSRVPFRAFRFDPGPLNRAFFQG
RFARALRRRQGLGESHRLVHGEADGLPGLVVDRFGEVLVLQVRSRGMEALREVWLPALLEVVAPKGVYERSDVEARRQEG
LPERVGVVYGEVPEVLEVEEDGLRFPIPLALAQKTGYYLDQRENRRLFEAMVRPGERVLDVYSYVGGFALRAARKGAYAL
AVDKDLEALGVLDQAALRLGLRVDIRHGEALPTLRGLEGPFHHVLLDPPTLVKRPEELPAMKRHLVDLVREALRLLAEEG
FLWLSSCSYHLRLEDLLEVARRAAADLGRRLRVHRVTYQPEDHPWSLHIPESLYLKTLVLQDDPL
>Mature_385_residues
MLGPVLRLVVKAGKERKLRNFYPNLYRDEIAAPPEGVGVAEAVDAEGHFLAVGYYDPRSRVPFRAFRFDPGPLNRAFFQG
RFARALRRRQGLGESHRLVHGEADGLPGLVVDRFGEVLVLQVRSRGMEALREVWLPALLEVVAPKGVYERSDVEARRQEG
LPERVGVVYGEVPEVLEVEEDGLRFPIPLALAQKTGYYLDQRENRRLFEAMVRPGERVLDVYSYVGGFALRAARKGAYAL
AVDKDLEALGVLDQAALRLGLRVDIRHGEALPTLRGLEGPFHHVLLDPPTLVKRPEELPAMKRHLVDLVREALRLLAEEG
FLWLSSCSYHLRLEDLLEVARRAAADLGRRLRVHRVTYQPEDHPWSLHIPESLYLKTLVLQDDPL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=390, Percent_Identity=34.8717948717949, Blast_Score=195, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43564; Mature: 43564

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGPVLRLVVKAGKERKLRNFYPNLYRDEIAAPPEGVGVAEAVDAEGHFLAVGYYDPRSR
CCHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCCCCCCCHHHCCCCCCEEEEEEECCCCC
VPFRAFRFDPGPLNRAFFQGRFARALRRRQGLGESHRLVHGEADGLPGLVVDRFGEVLVL
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHCCCEEEH
QVRSRGMEALREVWLPALLEVVAPKGVYERSDVEARRQEGLPERVGVVYGEVPEVLEVEE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHCC
DGLRFPIPLALAQKTGYYLDQRENRRLFEAMVRPGERVLDVYSYVGGFALRAARKGAYAL
CCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEE
AVDKDLEALGVLDQAALRLGLRVDIRHGEALPTLRGLEGPFHHVLLDPPTLVKRPEELPA
EECCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHCCCCCCCEEEECCHHHHCCCCCCHH
MKRHLVDLVREALRLLAEEGFLWLSSCSYHLRLEDLLEVARRAAADLGRRLRVHRVTYQP
HHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHEEEEEEEECC
EDHPWSLHIPESLYLKTLVLQDDPL
CCCCEEEECCHHHHHEEEEECCCCC
>Mature Secondary Structure
MLGPVLRLVVKAGKERKLRNFYPNLYRDEIAAPPEGVGVAEAVDAEGHFLAVGYYDPRSR
CCHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCCCCCCCHHHCCCCCCEEEEEEECCCCC
VPFRAFRFDPGPLNRAFFQGRFARALRRRQGLGESHRLVHGEADGLPGLVVDRFGEVLVL
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHCCCEEEH
QVRSRGMEALREVWLPALLEVVAPKGVYERSDVEARRQEGLPERVGVVYGEVPEVLEVEE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHCC
DGLRFPIPLALAQKTGYYLDQRENRRLFEAMVRPGERVLDVYSYVGGFALRAARKGAYAL
CCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEE
AVDKDLEALGVLDQAALRLGLRVDIRHGEALPTLRGLEGPFHHVLLDPPTLVKRPEELPA
EECCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHCCCCCCCEEEECCHHHHCCCCCCHH
MKRHLVDLVREALRLLAEEGFLWLSSCSYHLRLEDLLEVARRAAADLGRRLRVHRVTYQP
HHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHEEEEEEEECC
EDHPWSLHIPESLYLKTLVLQDDPL
CCCCEEEECCHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA