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The map label for this gene is trmFO [H]

Identifier: 55981411

GI number: 55981411

Start: 1370542

End: 1371873

Strand: Reverse

Name: trmFO [H]

Synonym: TTHA1442

Alternate gene names: 55981411

Gene position: 1371873-1370542 (Counterclockwise)

Preceding gene: 55981422

Following gene: 55981410

Centisome position: 74.17

GC content: 70.27

Gene sequence:

>1332_bases
ATGGAACGGGTGAACGTGGTGGGGGCGGGCCTCGCGGGGAGCGAGGCCGCCTGGACCCTCTTGCGCCTGGGCGTTCCCGT
GCGCCTTTTTGAGATGCGCCCCAAGCGGATGACCCCGGCCCACGGGACGGACCGCTTCGCCGAGATCGTCTGCTCCAACT
CCCTGGGCGGGGAAGGGGAGACCAACGCCAAGGGGCTTCTCCAGGCGGAGATGCGCCGGGCGGGAAGCCTCGTCATGGAG
GCGGCGGACCTCGCCCGAGTGCCCGCCGGGGGGGCCTTGGCCGTGGACCGGGAGGAGTTCAGCGGCTACATCACGGAAAG
GCTCACGGGCCACCCCCTCCTCGAGGTGGTGCGGGAGGAGGTGCGGGAGATCCCCCCTGGGATCACCGTGCTCGCCACGG
GGCCTTTGACCTCCGAGGCCCTGGCCGAGGCCCTGAAGCGGCGCTTTGGCGACCACTTCCTCGCCTACTACGACGCCGCA
AGCCCCATCGTCCTCTACGAGAGCATTGACCTCACGAAGTGCTTCCGCGCGGGCCGCTACGGCCAGAGCGCCGACTACCT
CAACTGCCCCATGACCGAGGAGGAGTACCGGCGCTTCCACCAGGCCCTCCTCGAGGCCCAGCGCCACACCCCCCACGACT
GGGAGAAGCTGGAGTTCTTTGAGGCCTGCGTCCCCGTGGAGGAGCTCGCCCGGAGGGGCTACCAGACCCTGCTCTTTGGC
CCCATGAAGCCCGTGGGGCTCGTGGACCCCCGCACGGGGAAGGAGCCCTTCGCCGTGGTCCAGCTCCGCCAGGAGGACAA
GGCGGGGCGGATGTGGAGCCTGGTGGGGTTCCAGACGGGGCTCAAGTGGCCCGAGCAGAAGCGCCTCATCCAGATGATCC
CCGGCCTGGAAAACGCCGAGATCGTCCGCTACGGGGTGATGCACCGGAACACCTACCTAAACGCCCCGAGGCTCCTTGGC
GAGACCCTGGAGTTCAGGGAGGCGGAGGGGCTTTACGCCGCCGGGGTGTTGGCCGGGGTGGAGGGGTACCTGGAGAGCGC
CGCCACGGGCTTCCTCGCCGGGCTCAACGCCGCGCGGAAGGCCCTGGGGCTTCCCCCCGTGGCGCCCCCGGAGGAGAGTA
TGCTGGGGGGGCTTGTGCGCTACCTGGCCACGGCGAACCCGGAGGGCTTCCAGCCCATGTACGCCAACTGGGGCCTGGTG
CCCCCGGTGGAGGGGAGGATGGGCAAGAAGGAGAAGCGCCAGGCCATGTACCGGAGGGGCCTCGAGGCCTTCTCCGCCTG
GCTTTCCGGCCTGAACCCGCCCCTTCCCCGCCCCGAGGCGGCCCTCGTCTGA

Upstream 100 bases:

>100_bases
CTGGGTGTAGAAGTCCTCCTCCACCCGGAACCGGTGCCGCACCACGGAGGCCATTATAGTCCACTTTCCCCTAGGCCTTT
CCGGTGCTACCATCTAAAGG

Downstream 100 bases:

>100_bases
GATGCCCCTCCGCGTCCTCCTCTTCGCCGTGCCCGAGAAGGACGAGGAGGCGGTGGCCGCCCTCCTGGCGGAGGCGGGGC
CCTGGGCCGCCTGGAGGAGC

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MERVNVVGAGLAGSEAAWTLLRLGVPVRLFEMRPKRMTPAHGTDRFAEIVCSNSLGGEGETNAKGLLQAEMRRAGSLVME
AADLARVPAGGALAVDREEFSGYITERLTGHPLLEVVREEVREIPPGITVLATGPLTSEALAEALKRRFGDHFLAYYDAA
SPIVLYESIDLTKCFRAGRYGQSADYLNCPMTEEEYRRFHQALLEAQRHTPHDWEKLEFFEACVPVEELARRGYQTLLFG
PMKPVGLVDPRTGKEPFAVVQLRQEDKAGRMWSLVGFQTGLKWPEQKRLIQMIPGLENAEIVRYGVMHRNTYLNAPRLLG
ETLEFREAEGLYAAGVLAGVEGYLESAATGFLAGLNAARKALGLPPVAPPEESMLGGLVRYLATANPEGFQPMYANWGLV
PPVEGRMGKKEKRQAMYRRGLEAFSAWLSGLNPPLPRPEAALV

Sequences:

>Translated_443_residues
MERVNVVGAGLAGSEAAWTLLRLGVPVRLFEMRPKRMTPAHGTDRFAEIVCSNSLGGEGETNAKGLLQAEMRRAGSLVME
AADLARVPAGGALAVDREEFSGYITERLTGHPLLEVVREEVREIPPGITVLATGPLTSEALAEALKRRFGDHFLAYYDAA
SPIVLYESIDLTKCFRAGRYGQSADYLNCPMTEEEYRRFHQALLEAQRHTPHDWEKLEFFEACVPVEELARRGYQTLLFG
PMKPVGLVDPRTGKEPFAVVQLRQEDKAGRMWSLVGFQTGLKWPEQKRLIQMIPGLENAEIVRYGVMHRNTYLNAPRLLG
ETLEFREAEGLYAAGVLAGVEGYLESAATGFLAGLNAARKALGLPPVAPPEESMLGGLVRYLATANPEGFQPMYANWGLV
PPVEGRMGKKEKRQAMYRRGLEAFSAWLSGLNPPLPRPEAALV
>Mature_443_residues
MERVNVVGAGLAGSEAAWTLLRLGVPVRLFEMRPKRMTPAHGTDRFAEIVCSNSLGGEGETNAKGLLQAEMRRAGSLVME
AADLARVPAGGALAVDREEFSGYITERLTGHPLLEVVREEVREIPPGITVLATGPLTSEALAEALKRRFGDHFLAYYDAA
SPIVLYESIDLTKCFRAGRYGQSADYLNCPMTEEEYRRFHQALLEAQRHTPHDWEKLEFFEACVPVEELARRGYQTLLFG
PMKPVGLVDPRTGKEPFAVVQLRQEDKAGRMWSLVGFQTGLKWPEQKRLIQMIPGLENAEIVRYGVMHRNTYLNAPRLLG
ETLEFREAEGLYAAGVLAGVEGYLESAATGFLAGLNAARKALGLPPVAPPEESMLGGLVRYLATANPEGFQPMYANWGLV
PPVEGRMGKKEKRQAMYRRGLEAFSAWLSGLNPPLPRPEAALV

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=110, Percent_Identity=40.9090909090909, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17534255, Length=92, Percent_Identity=42.3913043478261, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 48864; Mature: 48864

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERVNVVGAGLAGSEAAWTLLRLGVPVRLFEMRPKRMTPAHGTDRFAEIVCSNSLGGEGE
CCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
TNAKGLLQAEMRRAGSLVMEAADLARVPAGGALAVDREEFSGYITERLTGHPLLEVVREE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHCCCCHHHHHHHHH
VREIPPGITVLATGPLTSEALAEALKRRFGDHFLAYYDAASPIVLYESIDLTKCFRAGRY
HHHCCCCEEEEECCCCCHHHHHHHHHHHHCCHHEEEECCCCCEEEEECCCHHHHHHCCCC
GQSADYLNCPMTEEEYRRFHQALLEAQRHTPHDWEKLEFFEACVPVEELARRGYQTLLFG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCCCEEEEC
PMKPVGLVDPRTGKEPFAVVQLRQEDKAGRMWSLVGFQTGLKWPEQKRLIQMIPGLENAE
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHCCCCCHHHHHHHHCCCCCCCH
IVRYGVMHRNTYLNAPRLLGETLEFREAEGLYAAGVLAGVEGYLESAATGFLAGLNAARK
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGLPPVAPPEESMLGGLVRYLATANPEGFQPMYANWGLVPPVEGRMGKKEKRQAMYRRG
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LEAFSAWLSGLNPPLPRPEAALV
HHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MERVNVVGAGLAGSEAAWTLLRLGVPVRLFEMRPKRMTPAHGTDRFAEIVCSNSLGGEGE
CCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
TNAKGLLQAEMRRAGSLVMEAADLARVPAGGALAVDREEFSGYITERLTGHPLLEVVREE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHCCCCHHHHHHHHH
VREIPPGITVLATGPLTSEALAEALKRRFGDHFLAYYDAASPIVLYESIDLTKCFRAGRY
HHHCCCCEEEEECCCCCHHHHHHHHHHHHCCHHEEEECCCCCEEEEECCCHHHHHHCCCC
GQSADYLNCPMTEEEYRRFHQALLEAQRHTPHDWEKLEFFEACVPVEELARRGYQTLLFG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCCCEEEEC
PMKPVGLVDPRTGKEPFAVVQLRQEDKAGRMWSLVGFQTGLKWPEQKRLIQMIPGLENAE
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHCCCCCHHHHHHHHCCCCCCCH
IVRYGVMHRNTYLNAPRLLGETLEFREAEGLYAAGVLAGVEGYLESAATGFLAGLNAARK
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGLPPVAPPEESMLGGLVRYLATANPEGFQPMYANWGLVPPVEGRMGKKEKRQAMYRRG
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LEAFSAWLSGLNPPLPRPEAALV
HHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA