Click here to switch to the map view.

The map label for this gene is braF [H]

Identifier: 55981303

GI number: 55981303

Start: 1274009

End: 1274512

Strand: Direct

Name: braF [H]

Synonym: TTHA1334

Alternate gene names: 55981303

Gene position: 1274009-1274512 (Clockwise)

Preceding gene: 55981302

Following gene: 55981304

Centisome position: 68.87

GC content: 67.46

Gene sequence:

>504_bases
ATGACCGTCCTGGAGAACATCCTGGTGGGGCGGCACATCCACACCCGCGTCCCCTACCTGCACGCCCTCCTCCGCACCCC
CCTCGCCCGAAAGGAGGAGAGAAAGGCCCTGGAGGAGGCCTTGAGCCTCTTGGAGTACGTGGGCCTCCTGCACCGCAAGG
ACGAGCTCGCCCGCAACCTCCCCTACGGCGAGCAGAGGAAGCTGGAGATCGCCCGCGCCCTCGCCCTCAAGCCCAAGCTC
CTCCTCCTGGACGAGCCCGCCGCGGGCATGAACCCCAAGGAGACGGAGGCCCTGCAGGAGTTCATCCTGAAAATCCGCAG
CGAGATGGGGCTCACCATCCTCCTCATTGAGCACGACATGCGCCTCGTGATGCGCATCTCCGACCGGATCGCCGTGCTGG
ACTACGGCTCCAAGATCGCCGAAGGCAAGCCCGAGGAGGTGCGGACCAACCCCCGGGTCATCGAGGCCTACCTGGGCCGG
GGGGCCGCGGGAGGTGCCGCATGA

Upstream 100 bases:

>100_bases
GATCCTCTTCCTCGGCCAGGACATCACGGGCTCCACCCCCGACAAGGCGGCCAAGCTCGGCATCGGGCGCACCTTCCAGA
ACATCCGCCTCTTCGGGGCC

Downstream 100 bases:

>100_bases
GCCTCCTGGAGCTCAAAAACGTCCACACCTACTACGGGCACATCCACGCCCTGAAGGGCATCTCCCTCACGGTGGAGGAG
GGGGAGATCGTCACCCTCAT

Product: branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTVLENILVGRHIHTRVPYLHALLRTPLARKEERKALEEALSLLEYVGLLHRKDELARNLPYGEQRKLEIARALALKPKL
LLLDEPAAGMNPKETEALQEFILKIRSEMGLTILLIEHDMRLVMRISDRIAVLDYGSKIAEGKPEEVRTNPRVIEAYLGR
GAAGGAA

Sequences:

>Translated_167_residues
MTVLENILVGRHIHTRVPYLHALLRTPLARKEERKALEEALSLLEYVGLLHRKDELARNLPYGEQRKLEIARALALKPKL
LLLDEPAAGMNPKETEALQEFILKIRSEMGLTILLIEHDMRLVMRISDRIAVLDYGSKIAEGKPEEVRTNPRVIEAYLGR
GAAGGAA
>Mature_166_residues
TVLENILVGRHIHTRVPYLHALLRTPLARKEERKALEEALSLLEYVGLLHRKDELARNLPYGEQRKLEIARALALKPKLL
LLDEPAAGMNPKETEALQEFILKIRSEMGLTILLIEHDMRLVMRISDRIAVLDYGSKIAEGKPEEVRTNPRVIEAYLGRG
AAGGAA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI105990541, Length=124, Percent_Identity=35.4838709677419, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI1789864, Length=159, Percent_Identity=52.2012578616352, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI1786398, Length=148, Percent_Identity=37.1621621621622, Blast_Score=90, Evalue=7e-20,
Organism=Escherichia coli, GI1789593, Length=122, Percent_Identity=36.0655737704918, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1789032, Length=151, Percent_Identity=32.4503311258278, Blast_Score=78, Evalue=3e-16,
Organism=Escherichia coli, GI1787029, Length=154, Percent_Identity=31.8181818181818, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1790525, Length=160, Percent_Identity=30.625, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788450, Length=158, Percent_Identity=31.0126582278481, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790190, Length=160, Percent_Identity=30, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1789672, Length=140, Percent_Identity=27.8571428571429, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786872, Length=125, Percent_Identity=29.6, Blast_Score=73, Evalue=8e-15,
Organism=Escherichia coli, GI87081791, Length=111, Percent_Identity=34.2342342342342, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI1787548, Length=149, Percent_Identity=32.2147651006711, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1788165, Length=125, Percent_Identity=33.6, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1788761, Length=164, Percent_Identity=28.0487804878049, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1790544, Length=157, Percent_Identity=27.3885350318471, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI87081709, Length=132, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787370, Length=161, Percent_Identity=29.1925465838509, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1789962, Length=131, Percent_Identity=28.2442748091603, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1786345, Length=147, Percent_Identity=32.6530612244898, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1787500, Length=132, Percent_Identity=31.8181818181818, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1788225, Length=166, Percent_Identity=31.9277108433735, Blast_Score=67, Evalue=5e-13,
Organism=Escherichia coli, GI1787712, Length=160, Percent_Identity=26.25, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI87082268, Length=129, Percent_Identity=34.8837209302326, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788644, Length=154, Percent_Identity=31.8181818181818, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787758, Length=127, Percent_Identity=30.7086614173228, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1790162, Length=151, Percent_Identity=28.476821192053, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI145693107, Length=114, Percent_Identity=35.9649122807018, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1786563, Length=132, Percent_Identity=31.8181818181818, Blast_Score=63, Evalue=8e-12,
Organism=Escherichia coli, GI48995001, Length=140, Percent_Identity=32.1428571428571, Blast_Score=61, Evalue=3e-11,
Organism=Escherichia coli, GI1787164, Length=90, Percent_Identity=34.4444444444444, Blast_Score=61, Evalue=4e-11,
Organism=Escherichia coli, GI87081834, Length=127, Percent_Identity=29.1338582677165, Blast_Score=60, Evalue=6e-11,
Organism=Escherichia coli, GI48994997, Length=101, Percent_Identity=28.7128712871287, Blast_Score=60, Evalue=6e-11,
Organism=Escherichia coli, GI1786703, Length=121, Percent_Identity=30.5785123966942, Blast_Score=60, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71983516, Length=107, Percent_Identity=34.5794392523364, Blast_Score=73, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=107, Percent_Identity=34.5794392523364, Blast_Score=73, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71983523, Length=107, Percent_Identity=34.5794392523364, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 18803; Mature: 18672

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLENILVGRHIHTRVPYLHALLRTPLARKEERKALEEALSLLEYVGLLHRKDELARNL
CCHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PYGEQRKLEIARALALKPKLLLLDEPAAGMNPKETEALQEFILKIRSEMGLTILLIEHDM
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHH
RLVMRISDRIAVLDYGSKIAEGKPEEVRTNPRVIEAYLGRGAAGGAA
HHHHHHHCCEEEEECCCHHCCCCCHHHCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVLENILVGRHIHTRVPYLHALLRTPLARKEERKALEEALSLLEYVGLLHRKDELARNL
CHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PYGEQRKLEIARALALKPKLLLLDEPAAGMNPKETEALQEFILKIRSEMGLTILLIEHDM
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHH
RLVMRISDRIAVLDYGSKIAEGKPEEVRTNPRVIEAYLGRGAAGGAA
HHHHHHHCCEEEEECCCHHCCCCCHHHCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]