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The map label for this gene is yfkN [H]

Identifier: 55981297

GI number: 55981297

Start: 1266884

End: 1268542

Strand: Reverse

Name: yfkN [H]

Synonym: TTHA1328

Alternate gene names: 55981297

Gene position: 1268542-1266884 (Counterclockwise)

Preceding gene: 55981309

Following gene: 55981293

Centisome position: 68.58

GC content: 67.57

Gene sequence:

>1659_bases
GTGAAACGGCGGGAAGTGTTGCAGGCGGGTATGGCCCTAGGGGGGCTTGCGGGGCTCGGGCGGGCCCTGGCCCAGGGGGG
GTTCACCCTGACCCTGGTCCACACCAACGACACCCACGCCCACCTGGAGCCCGTGGAGCTCACCCTCTCCGGCGAGAAGA
CCCCCGTGGGGGGCGTGGCCCGGCGCGTGGCCCTTTTTGACCGCGTCTGGGCCCGGGCGAAAAACCCCCTCTTCCTGGAC
GCCGGGGACGTCTTCCAGGGCACCCTCTACTTCAACCAGTACCGGGGCCTGGCGGACCGGTACTTCATGCACCGGCTCCG
CTACCGGGCGATGGCCCTGGGGAACCACGAGTTTGACCTGGGCCCCGGGCCCTTGGCGGACTTCCTCAAGGGGGCGAGGT
TTAAGGTGGTCTCCGCCAACGTGGACGCAAGCCGGGAGCCGAGGCTCAAGGGCCTCTTCGCCCCTTACGCCGTGGTCGTG
GTGGGCGGGGAGCGGGTGGGGATCATCGGCCTCACCACCCCGGACACCAGGGAGATCTCCAACCCCGGGCCCACCGTGGC
CTTCCTGGACCCCTATGAGTCCGCCCAGAAGGCGGTCTACGAGCTCCTCGCCAAGGGGGTCAACAAGATCGTGGTCCTCT
CCCACCTGGGCTACGGCGAGGACCTGAAGCTCGCCCGGAGGCTCGTGGGGGTCCAGGTGATCGTGGGCGGCCACTCCCAC
ACCCTCTTGGGGAGCTTCCCCCACAAGGAGCTTTCCCCGGCTGGACCCTACCCCACGGTGGTGAAGAACCCCGAGGGGAA
GGACGTCCTGGTGGTCCAGGCCTGGGAGTGGGGGAAGGTGGTGGGCCTCTTGGAGGTCACCTTTGACGCCAAGGGGGAGC
TTCTCGCCTACAAGGGGGAGGCCCTCCTCATGACCCCCGAGGCCGCCCCCGAGGACTTCTTCGCCAAGGAGGCCCTCCTC
GCCTACGCCCAGCCGGTGATGGCCTTGATGCAGCAGGTGATCGCCGAGGCCAAGGTGGACCTCGTGGGGGAAAGGGCTGT
GGTGCGCAGGCGGGAGAGCAACCTGGGGAACCTCATCACGGATGGGATGCTCTGGAAGACGAGAAACGCCGGGACCCAGA
TCGCCCTGCAAAACGGCGGCGGCATCCGGGCCTCCATCCCCAAAGGCCCCATCACCGTGGGCAAGGTCTACGAGGTCCTG
CCCTTCGGGAACACCCTGGTGGTGATGGACCTCAAGGGCAAGGAGATCTTGGCCGCCCTGGAGAACGGGGTCTCCCAGTG
GGAGAACACCGCGGGGCGCTTCCTCCAGGTTTCGGGCCTCCGCTACGCCTTTGACCTCTCCCGCCCCGCGGGGAGCCGGG
TGGTGCGGGTGGAGGTGAAGACGGAGAAGGGGTATGTGCCCCTGGACCTCGAGGCCACCTACCGTGTGGTGGTGAACAAC
TTCATCGCCAACGGCGGCGACGGCTTCACCGTCCTCAAGGAGGCCCAGGGCTACCGGGTGGACACGGGCTTTAGTGACGC
TGAAAGCTTCATGGACTACCTCAAGGAGCTCAAGGTGGTGGAGGCGGGCCTCGAGGGCCGGATTGAGGTCCTGAACGAGC
CTAAAGGGGAAAGGCCCGCCTACTTCGCCTACCGGGTGCCGGGGCTCGTGGGGGTGTAG

Upstream 100 bases:

>100_bases
TCAGATGTCAAGCTTGGACGGATCAAATTGCCAATAAAGCCTTCACGCCTTGTTTATGCGGGCACGGCGGGGGTGGTAGA
CTGGAGCGGGAGGTGAAGCC

Downstream 100 bases:

>100_bases
CCCGAAAGGACGCCCCCCGGGGAGGCCTCCCCGGGGGTTTTTCCTTACTTCTTCAGCGGGCCGGGGCGAGGACGAGGACC
GGGTCCCCCACCCGCACCAC

Product: 5'-nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MKRREVLQAGMALGGLAGLGRALAQGGFTLTLVHTNDTHAHLEPVELTLSGEKTPVGGVARRVALFDRVWARAKNPLFLD
AGDVFQGTLYFNQYRGLADRYFMHRLRYRAMALGNHEFDLGPGPLADFLKGARFKVVSANVDASREPRLKGLFAPYAVVV
VGGERVGIIGLTTPDTREISNPGPTVAFLDPYESAQKAVYELLAKGVNKIVVLSHLGYGEDLKLARRLVGVQVIVGGHSH
TLLGSFPHKELSPAGPYPTVVKNPEGKDVLVVQAWEWGKVVGLLEVTFDAKGELLAYKGEALLMTPEAAPEDFFAKEALL
AYAQPVMALMQQVIAEAKVDLVGERAVVRRRESNLGNLITDGMLWKTRNAGTQIALQNGGGIRASIPKGPITVGKVYEVL
PFGNTLVVMDLKGKEILAALENGVSQWENTAGRFLQVSGLRYAFDLSRPAGSRVVRVEVKTEKGYVPLDLEATYRVVVNN
FIANGGDGFTVLKEAQGYRVDTGFSDAESFMDYLKELKVVEAGLEGRIEVLNEPKGERPAYFAYRVPGLVGV

Sequences:

>Translated_552_residues
MKRREVLQAGMALGGLAGLGRALAQGGFTLTLVHTNDTHAHLEPVELTLSGEKTPVGGVARRVALFDRVWARAKNPLFLD
AGDVFQGTLYFNQYRGLADRYFMHRLRYRAMALGNHEFDLGPGPLADFLKGARFKVVSANVDASREPRLKGLFAPYAVVV
VGGERVGIIGLTTPDTREISNPGPTVAFLDPYESAQKAVYELLAKGVNKIVVLSHLGYGEDLKLARRLVGVQVIVGGHSH
TLLGSFPHKELSPAGPYPTVVKNPEGKDVLVVQAWEWGKVVGLLEVTFDAKGELLAYKGEALLMTPEAAPEDFFAKEALL
AYAQPVMALMQQVIAEAKVDLVGERAVVRRRESNLGNLITDGMLWKTRNAGTQIALQNGGGIRASIPKGPITVGKVYEVL
PFGNTLVVMDLKGKEILAALENGVSQWENTAGRFLQVSGLRYAFDLSRPAGSRVVRVEVKTEKGYVPLDLEATYRVVVNN
FIANGGDGFTVLKEAQGYRVDTGFSDAESFMDYLKELKVVEAGLEGRIEVLNEPKGERPAYFAYRVPGLVGV
>Mature_552_residues
MKRREVLQAGMALGGLAGLGRALAQGGFTLTLVHTNDTHAHLEPVELTLSGEKTPVGGVARRVALFDRVWARAKNPLFLD
AGDVFQGTLYFNQYRGLADRYFMHRLRYRAMALGNHEFDLGPGPLADFLKGARFKVVSANVDASREPRLKGLFAPYAVVV
VGGERVGIIGLTTPDTREISNPGPTVAFLDPYESAQKAVYELLAKGVNKIVVLSHLGYGEDLKLARRLVGVQVIVGGHSH
TLLGSFPHKELSPAGPYPTVVKNPEGKDVLVVQAWEWGKVVGLLEVTFDAKGELLAYKGEALLMTPEAAPEDFFAKEALL
AYAQPVMALMQQVIAEAKVDLVGERAVVRRRESNLGNLITDGMLWKTRNAGTQIALQNGGGIRASIPKGPITVGKVYEVL
PFGNTLVVMDLKGKEILAALENGVSQWENTAGRFLQVSGLRYAFDLSRPAGSRVVRVEVKTEKGYVPLDLEATYRVVVNN
FIANGGDGFTVLKEAQGYRVDTGFSDAESFMDYLKELKVVEAGLEGRIEVLNEPKGERPAYFAYRVPGLVGV

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=538, Percent_Identity=38.6617100371747, Blast_Score=322, Evalue=4e-88,
Organism=Escherichia coli, GI1786687, Length=549, Percent_Identity=30.7832422586521, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1790658, Length=543, Percent_Identity=24.3093922651934, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI221329836, Length=536, Percent_Identity=33.3955223880597, Blast_Score=281, Evalue=9e-76,
Organism=Drosophila melanogaster, GI19922446, Length=535, Percent_Identity=35.7009345794393, Blast_Score=278, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24654424, Length=535, Percent_Identity=35.7009345794393, Blast_Score=278, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24641187, Length=545, Percent_Identity=33.5779816513761, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI28573524, Length=531, Percent_Identity=33.5216572504708, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI19922444, Length=539, Percent_Identity=33.5807050092764, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19921980, Length=473, Percent_Identity=28.7526427061311, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24652512, Length=473, Percent_Identity=28.7526427061311, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI161076508, Length=473, Percent_Identity=28.7526427061311, Blast_Score=166, Evalue=5e-41,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 59971; Mature: 59971

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRREVLQAGMALGGLAGLGRALAQGGFTLTLVHTNDTHAHLEPVELTLSGEKTPVGGVA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEECEEEEEECCCCCCCHHHH
RRVALFDRVWARAKNPLFLDAGDVFQGTLYFNQYRGLADRYFMHRLRYRAMALGNHEFDL
HHHHHHHHHHHHCCCCEEEECCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
GPGPLADFLKGARFKVVSANVDASREPRLKGLFAPYAVVVVGGERVGIIGLTTPDTREIS
CCCCHHHHHCCCEEEEEEECCCCCCCCCCCEECCCEEEEEECCCEEEEEEECCCCCCCCC
NPGPTVAFLDPYESAQKAVYELLAKGVNKIVVLSHLGYGEDLKLARRLVGVQVIVGGHSH
CCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCEEEEECCCCC
TLLGSFPHKELSPAGPYPTVVKNPEGKDVLVVQAWEWGKVVGLLEVTFDAKGELLAYKGE
EEECCCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEEECC
ALLMTPEAAPEDFFAKEALLAYAQPVMALMQQVIAEAKVDLVGERAVVRRRESNLGNLIT
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHC
DGMLWKTRNAGTQIALQNGGGIRASIPKGPITVGKVYEVLPFGNTLVVMDLKGKEILAAL
CCEEEEECCCCCEEEEECCCCEEEECCCCCCCHHHHEEEEECCCEEEEEECCCHHHHHHH
ENGVSQWENTAGRFLQVSGLRYAFDLSRPAGSRVVRVEVKTEKGYVPLDLEATYRVVVNN
HHHHHHHHHCCCCEEEECCCEEEEECCCCCCCEEEEEEEEECCCEEEEECCCHHHHHHHH
FIANGGDGFTVLKEAQGYRVDTGFSDAESFMDYLKELKVVEAGLEGRIEVLNEPKGERPA
HHCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCE
YFAYRVPGLVGV
EEEEECCCCCCC
>Mature Secondary Structure
MKRREVLQAGMALGGLAGLGRALAQGGFTLTLVHTNDTHAHLEPVELTLSGEKTPVGGVA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEECEEEEEECCCCCCCHHHH
RRVALFDRVWARAKNPLFLDAGDVFQGTLYFNQYRGLADRYFMHRLRYRAMALGNHEFDL
HHHHHHHHHHHHCCCCEEEECCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
GPGPLADFLKGARFKVVSANVDASREPRLKGLFAPYAVVVVGGERVGIIGLTTPDTREIS
CCCCHHHHHCCCEEEEEEECCCCCCCCCCCEECCCEEEEEECCCEEEEEEECCCCCCCCC
NPGPTVAFLDPYESAQKAVYELLAKGVNKIVVLSHLGYGEDLKLARRLVGVQVIVGGHSH
CCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCEEEEECCCCC
TLLGSFPHKELSPAGPYPTVVKNPEGKDVLVVQAWEWGKVVGLLEVTFDAKGELLAYKGE
EEECCCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEEECC
ALLMTPEAAPEDFFAKEALLAYAQPVMALMQQVIAEAKVDLVGERAVVRRRESNLGNLIT
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHC
DGMLWKTRNAGTQIALQNGGGIRASIPKGPITVGKVYEVLPFGNTLVVMDLKGKEILAAL
CCEEEEECCCCCEEEEECCCCEEEECCCCCCCHHHHEEEEECCCEEEEEECCCHHHHHHH
ENGVSQWENTAGRFLQVSGLRYAFDLSRPAGSRVVRVEVKTEKGYVPLDLEATYRVVVNN
HHHHHHHHHCCCCEEEECCCEEEEECCCCCCCEEEEEEEEECCCEEEEECCCHHHHHHHH
FIANGGDGFTVLKEAQGYRVDTGFSDAESFMDYLKELKVVEAGLEGRIEVLNEPKGERPA
HHCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCE
YFAYRVPGLVGV
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]