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The map label for this gene is leuC

Identifier: 55981197

GI number: 55981197

Start: 1166248

End: 1167666

Strand: Direct

Name: leuC

Synonym: TTHA1228

Alternate gene names: 55981197

Gene position: 1166248-1167666 (Clockwise)

Preceding gene: 55981193

Following gene: 55981198

Centisome position: 63.05

GC content: 67.86

Gene sequence:

>1419_bases
ATGGGAAAGACGCTCTACGAGAAGGTTTGGGAAGCCCACGAGGTGAGGAAGCTCAAAAACGGCCAAAGCCAGCTCTTCAT
AGACCTCCACCTCCTCCACGAGGTCACGAGCCCCCAGGCCTTCGGGATGCTCAAGGACCTTGGCCTTCGGGTACGCTACC
CCCACCGCACCTTCGCCACCGTGGACCACATCGTCCCCACCCACGACCGCACCGAGCCCTTCCAGGACCCTCTGGCCCAG
AGCATGCTGGAGGCCCTCAGGGCGAACACAAGGGAGCACGGCATCACCTTCTTTGACCTGGGAAGCGGCAACCAAGGCAT
CGTCCACGTCATCGGGCCCCAGCTCGGCCTCACCCAGCCCGGGATGACCATCGCCTGCGGGGACTCCCACACCTCCACCC
ACGGGGCCTTCGGGGCCGTGGCCTTCGGCATCGGCACGAGCCAGGTCCGGGACGTCCTCGCCACCCAGACCCTCGCCGCC
CAAAAGCTCAAGGTGCGGCGGATCAACGTGGAGGGGAGGCTCGCCCCCGGGGTCTACGCCAAGGACGTGATCCTCCACAT
CATCCGCCACCTGGGGGTGAAGGGGGGCTTGGGCTACGCCTATGAATACGGGGGAAGCGCCGTGGAGGCCATGGACATGG
AAAGCCGCATGACCCTCTGCAACATGTCCATTGAGGGCGGAGCCCGCATCGGCTACGTGAACCCCGACGAGACCACCTTC
CAGTACCTCGAGGGCCGCCCCTACGTCCCCAAGGGGTCCGAGTGGGAGGAGGCCAAGAGGAGGTGGCTCGCTTGGCGCTC
CGACCCCGACGCCTCCTACGACGACGTGGTCACCTTCCGCGCCGAGGAGATCGCCCCCACCGTCACCTGGGGCATCACCC
CGGGCCAGGCCATCCCCATTGACGGCAGGATCCCCCTCCTGGAGGAGCTCCCCGAGGAGGAGCGCCCCGTGGCCGAGGAG
GCTTTGGCCTACATGGGCTTCAGGCCGGGCCAGCCCATCAAGGGGGTGCCCATCCAGGTGGCCTTCATCGGAAGCTGCAC
CAACGCCAGGCTTTCCGACCTCCGGGAGGTGGCCCGCTACCTCAAGGGGCACAAGGTGAAGAAGGGGGTGCGGGCCCTGG
TGGTGCCGGGCTCGGAGTGGGTGGCGAGGAAGGCCGAGGAGGAGGGGATCGCCGAGGTCTTCCGCGAGGCGGGGTTTGAA
TGGCGGATGCCCGGCTGCTCCATGTGCCTCGCCATGAACCCGGACCGGCTGGAAGGGGACGAGCTTTGCGCCAGCAGCTC
CAACCGGAACTACAAGGGACGCATGGGAAGCCCCAGGGGCCGCACCGTCCTCATGAGCCCCCTCATGGTGGCGGCGGCGG
CCGTGGCCGGGGAGATCGCCGACGCCCGGGAGGTCTTCGGCCTGGCGGGCGTCCGCTGA

Upstream 100 bases:

>100_bases
GGTGATCCTAGTCCTAGACCGGGCGGGCCCGGTCTAGGGATGGCCGTCGCGTATCCCCCGGGCCCCAAAGGACCCGGGGG
TTTTGCGTAAGGGGGGTTTC

Downstream 100 bases:

>100_bases
GGAGGGAAAAGCCATGCTGGAGAAGTTCACCGTGATCCGCGGAAAAGCGGTGCCCTTGAGGGGCGAGGACATTGACACGG
ACCGGATCCTCCCCGCCCGC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MGKTLYEKVWEAHEVRKLKNGQSQLFIDLHLLHEVTSPQAFGMLKDLGLRVRYPHRTFATVDHIVPTHDRTEPFQDPLAQ
SMLEALRANTREHGITFFDLGSGNQGIVHVIGPQLGLTQPGMTIACGDSHTSTHGAFGAVAFGIGTSQVRDVLATQTLAA
QKLKVRRINVEGRLAPGVYAKDVILHIIRHLGVKGGLGYAYEYGGSAVEAMDMESRMTLCNMSIEGGARIGYVNPDETTF
QYLEGRPYVPKGSEWEEAKRRWLAWRSDPDASYDDVVTFRAEEIAPTVTWGITPGQAIPIDGRIPLLEELPEEERPVAEE
ALAYMGFRPGQPIKGVPIQVAFIGSCTNARLSDLREVARYLKGHKVKKGVRALVVPGSEWVARKAEEEGIAEVFREAGFE
WRMPGCSMCLAMNPDRLEGDELCASSSNRNYKGRMGSPRGRTVLMSPLMVAAAAVAGEIADAREVFGLAGVR

Sequences:

>Translated_472_residues
MGKTLYEKVWEAHEVRKLKNGQSQLFIDLHLLHEVTSPQAFGMLKDLGLRVRYPHRTFATVDHIVPTHDRTEPFQDPLAQ
SMLEALRANTREHGITFFDLGSGNQGIVHVIGPQLGLTQPGMTIACGDSHTSTHGAFGAVAFGIGTSQVRDVLATQTLAA
QKLKVRRINVEGRLAPGVYAKDVILHIIRHLGVKGGLGYAYEYGGSAVEAMDMESRMTLCNMSIEGGARIGYVNPDETTF
QYLEGRPYVPKGSEWEEAKRRWLAWRSDPDASYDDVVTFRAEEIAPTVTWGITPGQAIPIDGRIPLLEELPEEERPVAEE
ALAYMGFRPGQPIKGVPIQVAFIGSCTNARLSDLREVARYLKGHKVKKGVRALVVPGSEWVARKAEEEGIAEVFREAGFE
WRMPGCSMCLAMNPDRLEGDELCASSSNRNYKGRMGSPRGRTVLMSPLMVAAAAVAGEIADAREVFGLAGVR
>Mature_471_residues
GKTLYEKVWEAHEVRKLKNGQSQLFIDLHLLHEVTSPQAFGMLKDLGLRVRYPHRTFATVDHIVPTHDRTEPFQDPLAQS
MLEALRANTREHGITFFDLGSGNQGIVHVIGPQLGLTQPGMTIACGDSHTSTHGAFGAVAFGIGTSQVRDVLATQTLAAQ
KLKVRRINVEGRLAPGVYAKDVILHIIRHLGVKGGLGYAYEYGGSAVEAMDMESRMTLCNMSIEGGARIGYVNPDETTFQ
YLEGRPYVPKGSEWEEAKRRWLAWRSDPDASYDDVVTFRAEEIAPTVTWGITPGQAIPIDGRIPLLEELPEEERPVAEEA
LAYMGFRPGQPIKGVPIQVAFIGSCTNARLSDLREVARYLKGHKVKKGVRALVVPGSEWVARKAEEEGIAEVFREAGFEW
RMPGCSMCLAMNPDRLEGDELCASSSNRNYKGRMGSPRGRTVLMSPLMVAAAAVAGEIADAREVFGLAGVR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=401, Percent_Identity=27.6807980049875, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI41352693, Length=370, Percent_Identity=23.7837837837838, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=53.862660944206, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI2367097, Length=110, Percent_Identity=32.7272727272727, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32564738, Length=392, Percent_Identity=28.8265306122449, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25149337, Length=392, Percent_Identity=28.8265306122449, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=319, Percent_Identity=29.153605015674, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=373, Percent_Identity=22.7882037533512, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=49.3617021276596, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322261, Length=411, Percent_Identity=27.4939172749392, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=391, Percent_Identity=28.6445012787724, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=495, Percent_Identity=26.0606060606061, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI161076999, Length=389, Percent_Identity=28.2776349614396, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281365315, Length=389, Percent_Identity=28.2776349614396, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17864292, Length=389, Percent_Identity=28.2776349614396, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28571643, Length=473, Percent_Identity=26.4270613107822, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24645686, Length=379, Percent_Identity=26.6490765171504, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137564, Length=368, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_THET2 (Q72JB3)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_004836.1
- ProteinModelPortal:   Q72JB3
- SMR:   Q72JB3
- STRING:   Q72JB3
- GeneID:   2774847
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q72JB3
- ProtClustDB:   PRK05478
- BioCyc:   TTHE262724:TT_C0865-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51838; Mature: 51707

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTLYEKVWEAHEVRKLKNGQSQLFIDLHLLHEVTSPQAFGMLKDLGLRVRYPHRTFAT
CCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCHHHHHHHHCCCEEECCCHHHHH
VDHIVPTHDRTEPFQDPLAQSMLEALRANTREHGITFFDLGSGNQGIVHVIGPQLGLTQP
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCEEEEEECCCCCCEEEEECCCCCCCCC
GMTIACGDSHTSTHGAFGAVAFGIGTSQVRDVLATQTLAAQKLKVRRINVEGRLAPGVYA
CCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHH
KDVILHIIRHLGVKGGLGYAYEYGGSAVEAMDMESRMTLCNMSIEGGARIGYVNPDETTF
HHHHHHHHHHCCCCCCCCEEEECCCCEEEHHHHHHHHEEEEEEECCCCEEEECCCCCHHH
QYLEGRPYVPKGSEWEEAKRRWLAWRSDPDASYDDVVTFRAEEIAPTVTWGITPGQAIPI
HHHCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCEEEEEHHHCCCEEEECCCCCCEECC
DGRIPLLEELPEEERPVAEEALAYMGFRPGQPIKGVPIQVAFIGSCTNARLSDLREVARY
CCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
LKGHKVKKGVRALVVPGSEWVARKAEEEGIAEVFREAGFEWRMPGCSMCLAMNPDRLEGD
HCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEECCCCCCCHH
ELCASSSNRNYKGRMGSPRGRTVLMSPLMVAAAAVAGEIADAREVFGLAGVR
HHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GKTLYEKVWEAHEVRKLKNGQSQLFIDLHLLHEVTSPQAFGMLKDLGLRVRYPHRTFAT
CCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCHHHHHHHHCCCEEECCCHHHHH
VDHIVPTHDRTEPFQDPLAQSMLEALRANTREHGITFFDLGSGNQGIVHVIGPQLGLTQP
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCEEEEEECCCCCCEEEEECCCCCCCCC
GMTIACGDSHTSTHGAFGAVAFGIGTSQVRDVLATQTLAAQKLKVRRINVEGRLAPGVYA
CCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHH
KDVILHIIRHLGVKGGLGYAYEYGGSAVEAMDMESRMTLCNMSIEGGARIGYVNPDETTF
HHHHHHHHHHCCCCCCCCEEEECCCCEEEHHHHHHHHEEEEEEECCCCEEEECCCCCHHH
QYLEGRPYVPKGSEWEEAKRRWLAWRSDPDASYDDVVTFRAEEIAPTVTWGITPGQAIPI
HHHCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCEEEEEHHHCCCEEEECCCCCCEECC
DGRIPLLEELPEEERPVAEEALAYMGFRPGQPIKGVPIQVAFIGSCTNARLSDLREVARY
CCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
LKGHKVKKGVRALVVPGSEWVARKAEEEGIAEVFREAGFEWRMPGCSMCLAMNPDRLEGD
HCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEECCCCCCCHH
ELCASSSNRNYKGRMGSPRGRTVLMSPLMVAAAAVAGEIADAREVFGLAGVR
HHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA