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The map label for this gene is ilvB [H]

Identifier: 55981182

GI number: 55981182

Start: 1154495

End: 1156183

Strand: Reverse

Name: ilvB [H]

Synonym: TTHA1213

Alternate gene names: 55981182

Gene position: 1156183-1154495 (Counterclockwise)

Preceding gene: 55981183

Following gene: 55981181

Centisome position: 62.51

GC content: 68.5

Gene sequence:

>1689_bases
ATGAAGGGAGCGGAGGCACTTTTAAGGGCGCTGGAGCGGGAAGGGGTGGAGGTGATCTTCGGCCACCCGGGCGGGGCGAT
CATGCCCACCTACGACGCCCTCTACGACAGCCCCATCCGCCACATCCTGGTGCGGCACGAGCAGGGCGGGGTCCATGCGG
CCACCGCCTACGCCCGCGCCTCAGGCCGGGTGGGGGTGGTCATGGCCACCTCGGGCCCCGGGGCCCTGAACCTGGTCACG
GGCCTCGCGGACGCCATGATGGACTCCACCCCGGTGGTGGCCATCACCGGGAACGTCCCCCGGGCCCTCATCGGCACCGA
CGCCTTCCAGGAGGCGGACGTCACCGGGGTTACCATGCCCATCACCAAGCACAACTACCTGGTCCAGGACGTGAACGAGA
TCCCCCGGGTGGTGAAGGAGGCCTTCCACATCGCCGCCACCGGCCGCCCCGGCCCCGTGCTCATTGACATCCCCAAGGAC
GTGCAGCTTGCGGAGTTCACGGGGAGCTTTGACGTGAAGCTGGACCTCCCCGGGTACAAGCCCACCCTGAAGGGGCACCC
CAAGCAGATTGAGAGGGCCCTGGACGCCCTGGAGAAGGCGGAGCGCCCCGTCCTCATGGTGGGGGGTGGGGCGCAGCACG
CCCACGCCGAGCTCCTCGCCTTCGCCGAGAAGACGGGGCTTCCCGTGATCACCACCCTCATGGGCCTCGGGGCCTTCCCC
GGGAACCACCCCTTGTGGCTCGGGATGCCGGGGATGCACGGCACCGTGGCCGCCAACCGGGCCATCCACCACGCGGACGT
GATCCTCGCCATCGGCCTCCGCTTTGACGACCGGGTCACGGGCAAGGTCTCCCGCTTCGCCCCCCACGCCCACACCATCA
TCCACGTGGACATAGACCCCGCCGAGATCGGCAAGGTGGTGCGCACCCACATCCCCATCGTGGGGGACTCGAGGCTCGTC
CTCAAGGAGCTCCTCAAGGGGGCGAAGGCCTTGAGGCTTGCCGCCTGGTGGCGGGAGCTCGAGGAGTGGCGCACCCGCTA
CCCCTTGCGCTACAGGCCCAAGCCCCACCTGCAGAGCCAGGAGGTGATCCGGGCCTTCTACGAGGCCACGGGAGGACACG
CCATCGTCACCACGGGGGTGGGGCAGCACCAGATGTTCGCCGCCCAGTTCTTCCCCGTGACGAGGCCGAGGAGCTTCCTC
ACCAGTGGGGGCCTCGGCACCATGGGGGTGGGGCTTCCCTTCGCCATCGGGGCCAAGATCGCCCGCCCTGAGGAGCTCGT
CATTGACTTTGACGGGGACGGCTCCTTCCAGATGACCCTGCAGGAGCTGGCCACGGTGGTGAAGTACAAGCTGGACGTGA
AGGTCGTCATCCTGAACAACGGCTACCTGGGCATGGTGCGCCAGTGGCAGGACCTCTTCCACGCCAAGCGCTACAGCGAG
GTCTACCTGGCGGACTCCAACCCCGACTTCGCCCGCCTCGCCGAGGCCTACGGGATCAAGGGGGTGAGGGTGGAGCGGAA
GGAGGACCTCATGAAGGGGGTGGAGGCCGTCCTGAACGCCGACGGCCCCGTGGTGGCCGAGTTCAAGGTCTACCAGGAGG
AGGGGGTCTTCCCCATGATCCCCGCCGGGGGCGCGGCCGAGGACATGATCCTCGAGCCTCCCGAGGAGAAGGAGGAGGTG
GGGGCGTGA

Upstream 100 bases:

>100_bases
GTCCTAATCGTAGGGCCAGCGGGGGGTAGGGCGTAGCGCTTTAGGGCGCATCCCATAACCCCCCGGGCCAACCGGGGGTT
TCAAGTTTAAGGAGGGGTCC

Downstream 100 bases:

>100_bases
GGCACGTCATCTCCGTTTTGGTGCAGGACCACCCCAGGGTCTTGAACCGCATCACGGGCCTTTTCGCAAGGCGCGGCTTC
AACCTGGAAAGCCTCGCCGT

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MKGAEALLRALEREGVEVIFGHPGGAIMPTYDALYDSPIRHILVRHEQGGVHAATAYARASGRVGVVMATSGPGALNLVT
GLADAMMDSTPVVAITGNVPRALIGTDAFQEADVTGVTMPITKHNYLVQDVNEIPRVVKEAFHIAATGRPGPVLIDIPKD
VQLAEFTGSFDVKLDLPGYKPTLKGHPKQIERALDALEKAERPVLMVGGGAQHAHAELLAFAEKTGLPVITTLMGLGAFP
GNHPLWLGMPGMHGTVAANRAIHHADVILAIGLRFDDRVTGKVSRFAPHAHTIIHVDIDPAEIGKVVRTHIPIVGDSRLV
LKELLKGAKALRLAAWWRELEEWRTRYPLRYRPKPHLQSQEVIRAFYEATGGHAIVTTGVGQHQMFAAQFFPVTRPRSFL
TSGGLGTMGVGLPFAIGAKIARPEELVIDFDGDGSFQMTLQELATVVKYKLDVKVVILNNGYLGMVRQWQDLFHAKRYSE
VYLADSNPDFARLAEAYGIKGVRVERKEDLMKGVEAVLNADGPVVAEFKVYQEEGVFPMIPAGGAAEDMILEPPEEKEEV
GA

Sequences:

>Translated_562_residues
MKGAEALLRALEREGVEVIFGHPGGAIMPTYDALYDSPIRHILVRHEQGGVHAATAYARASGRVGVVMATSGPGALNLVT
GLADAMMDSTPVVAITGNVPRALIGTDAFQEADVTGVTMPITKHNYLVQDVNEIPRVVKEAFHIAATGRPGPVLIDIPKD
VQLAEFTGSFDVKLDLPGYKPTLKGHPKQIERALDALEKAERPVLMVGGGAQHAHAELLAFAEKTGLPVITTLMGLGAFP
GNHPLWLGMPGMHGTVAANRAIHHADVILAIGLRFDDRVTGKVSRFAPHAHTIIHVDIDPAEIGKVVRTHIPIVGDSRLV
LKELLKGAKALRLAAWWRELEEWRTRYPLRYRPKPHLQSQEVIRAFYEATGGHAIVTTGVGQHQMFAAQFFPVTRPRSFL
TSGGLGTMGVGLPFAIGAKIARPEELVIDFDGDGSFQMTLQELATVVKYKLDVKVVILNNGYLGMVRQWQDLFHAKRYSE
VYLADSNPDFARLAEAYGIKGVRVERKEDLMKGVEAVLNADGPVVAEFKVYQEEGVFPMIPAGGAAEDMILEPPEEKEEV
GA
>Mature_562_residues
MKGAEALLRALEREGVEVIFGHPGGAIMPTYDALYDSPIRHILVRHEQGGVHAATAYARASGRVGVVMATSGPGALNLVT
GLADAMMDSTPVVAITGNVPRALIGTDAFQEADVTGVTMPITKHNYLVQDVNEIPRVVKEAFHIAATGRPGPVLIDIPKD
VQLAEFTGSFDVKLDLPGYKPTLKGHPKQIERALDALEKAERPVLMVGGGAQHAHAELLAFAEKTGLPVITTLMGLGAFP
GNHPLWLGMPGMHGTVAANRAIHHADVILAIGLRFDDRVTGKVSRFAPHAHTIIHVDIDPAEIGKVVRTHIPIVGDSRLV
LKELLKGAKALRLAAWWRELEEWRTRYPLRYRPKPHLQSQEVIRAFYEATGGHAIVTTGVGQHQMFAAQFFPVTRPRSFL
TSGGLGTMGVGLPFAIGAKIARPEELVIDFDGDGSFQMTLQELATVVKYKLDVKVVILNNGYLGMVRQWQDLFHAKRYSE
VYLADSNPDFARLAEAYGIKGVRVERKEDLMKGVEAVLNADGPVVAEFKVYQEEGVFPMIPAGGAAEDMILEPPEEKEEV
GA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=549, Percent_Identity=27.3224043715847, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI21361361, Length=562, Percent_Identity=27.7580071174377, Blast_Score=165, Evalue=1e-40,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=47.9020979020979, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=44.4444444444444, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786717, Length=536, Percent_Identity=36.0074626865672, Blast_Score=324, Evalue=1e-89,
Organism=Escherichia coli, GI1787096, Length=547, Percent_Identity=30.3473491773309, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788716, Length=510, Percent_Identity=25.8823529411765, Blast_Score=154, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=557, Percent_Identity=28.7253141831239, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=557, Percent_Identity=28.7253141831239, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=574, Percent_Identity=25.609756097561, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=588, Percent_Identity=44.2176870748299, Blast_Score=475, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320816, Length=470, Percent_Identity=24.8936170212766, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323163, Length=492, Percent_Identity=22.5609756097561, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922626, Length=553, Percent_Identity=28.5714285714286, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60982; Mature: 60982

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGAEALLRALEREGVEVIFGHPGGAIMPTYDALYDSPIRHILVRHEQGGVHAATAYARA
CCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCCHHHHEEEECCCCEEHHHHHHHC
SGRVGVVMATSGPGALNLVTGLADAMMDSTPVVAITGNVPRALIGTDAFQEADVTGVTMP
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCCEEEEE
ITKHNYLVQDVNEIPRVVKEAFHIAATGRPGPVLIDIPKDVQLAEFTGSFDVKLDLPGYK
EECCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCEEEEECCCEEEEEECCCCC
PTLKGHPKQIERALDALEKAERPVLMVGGGAQHAHAELLAFAEKTGLPVITTLMGLGAFP
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCC
GNHPLWLGMPGMHGTVAANRAIHHADVILAIGLRFDDRVTGKVSRFAPHAHTIIHVDIDP
CCCCEEEECCCCCCHHHHCCCHHHCEEEEEEECCCCCCCCCCHHHCCCCCEEEEEEECCH
AEIGKVVRTHIPIVGDSRLVLKELLKGAKALRLAAWWRELEEWRTRYPLRYRPKPHLQSQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHH
EVIRAFYEATGGHAIVTTGVGQHQMFAAQFFPVTRPRSFLTSGGLGTMGVGLPFAIGAKI
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHCCCC
ARPEELVIDFDGDGSFQMTLQELATVVKYKLDVKVVILNNGYLGMVRQWQDLFHAKRYSE
CCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCE
VYLADSNPDFARLAEAYGIKGVRVERKEDLMKGVEAVLNADGPVVAEFKVYQEEGVFPMI
EEEECCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCEEEE
PAGGAAEDMILEPPEEKEEVGA
CCCCCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MKGAEALLRALEREGVEVIFGHPGGAIMPTYDALYDSPIRHILVRHEQGGVHAATAYARA
CCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCCHHHHEEEECCCCEEHHHHHHHC
SGRVGVVMATSGPGALNLVTGLADAMMDSTPVVAITGNVPRALIGTDAFQEADVTGVTMP
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCCEEEEE
ITKHNYLVQDVNEIPRVVKEAFHIAATGRPGPVLIDIPKDVQLAEFTGSFDVKLDLPGYK
EECCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCEEEEECCCEEEEEECCCCC
PTLKGHPKQIERALDALEKAERPVLMVGGGAQHAHAELLAFAEKTGLPVITTLMGLGAFP
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCC
GNHPLWLGMPGMHGTVAANRAIHHADVILAIGLRFDDRVTGKVSRFAPHAHTIIHVDIDP
CCCCEEEECCCCCCHHHHCCCHHHCEEEEEEECCCCCCCCCCHHHCCCCCEEEEEEECCH
AEIGKVVRTHIPIVGDSRLVLKELLKGAKALRLAAWWRELEEWRTRYPLRYRPKPHLQSQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHH
EVIRAFYEATGGHAIVTTGVGQHQMFAAQFFPVTRPRSFLTSGGLGTMGVGLPFAIGAKI
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHCCCC
ARPEELVIDFDGDGSFQMTLQELATVVKYKLDVKVVILNNGYLGMVRQWQDLFHAKRYSE
CCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCE
VYLADSNPDFARLAEAYGIKGVRVERKEDLMKGVEAVLNADGPVVAEFKVYQEEGVFPMI
EEEECCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCEEEE
PAGGAAEDMILEPPEEKEEVGA
CCCCCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]