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The map label for this gene is leuA [H]

Identifier: 55981179

GI number: 55981179

Start: 1151356

End: 1152918

Strand: Reverse

Name: leuA [H]

Synonym: TTHA1210

Alternate gene names: 55981179

Gene position: 1152918-1151356 (Counterclockwise)

Preceding gene: 55981180

Following gene: 55981178

Centisome position: 62.33

GC content: 67.43

Gene sequence:

>1563_bases
ATGGAGAAGGAACGGCACATCCGGATCTTTGACACCACGCTCAGGGACGGGGAGCAAAGCCCAGGGGTGGCCCTCTCCCT
GGACCAGAAGCTGGAGATCGCCCAGGCCCTCGCCCAGCTCAACGTGGACATCATTGAGGCGGGCTTCCCCGTATCGGGGC
CCATGGAGTTTGAGGCGGTGCGCCGCATCGCCACCGAGGTCAAGGGCCCCATCATCGCCGCCCTCGCCCGCACCCACACC
CTGGACATTGACCAGGCGGCCAAGGCCCTGGAGAAGGCGGAGAAGCCCAGGATCCACGTCTTCACCTCCGCCTCCAAGGT
CCACCTGCAGTACATGCTGAGGAAGACGGAGGAGGAGGTCCTGGAGATGGCGGATAGAATGGTCCGCTACGCCAGGAGGT
ACGTGGACGACGTGGAGTTCTCCGCCCAGGACGTGATGCGGGCGGACTGGGAGTTCGTCAAGAGGCTTTACGAGGTGGCC
ATTGAGGCCGGGGCCACCACCATCAACATCCCCGACACCACGGGCTACGGCACGCCCCAGGAGTACGGGGCCCTGATCCG
CAGGATCCGGGACGAGGTGGTGCGGGGGCGGGACGTGGTCATCTCCACCCACACCCACGACGACCTGGGCATGGCCACGG
CCAACGCCCTGGCGGGCGTGGAGAACGGGGCGGGCCAGATTGAGTGCACCATCAACGGCATCGGGGAGCGGGCGGGGAAC
TGCGCCCTGGAGGAGGTGGTCATGGCCCTCTACGTCCGCCGGGACTGGTACAAGGCCTACACGCGGATCAACACCCGGGA
GATCTACCGGGTGAGCCGCCTGGTGGAGCGCTACACCGGGATGCCCGTCCCCCCCAACAAGGCCATCGTGGGGGACAACG
CCTTCGCCCACGAGTCGGGGATCCACCAGGACGGGGTCATCAAGCACCGGGCCACCTACGAGATCATGGACGCCGAGCTC
ATCGGGAGGCGGCCCGCCGTGCTCGTCCTCGGCAAGCACTCGGGCCGGGCGGCTTTTAAAAAGGCCCTCGAGGACCTGGG
CTACAAGGACCTCTCCGAGGAGGAGGTGAAGAAGCTCTTCGCCCGCTTCAAGGAGATCGCCGAGAAGAAGGGGCCCCTTT
CCGCCGAGGAGCTCCAGGCCCTGGTGGAAAGCGAGCGGGAGCCCACCTCCCACTTCTTCCAGCTGGAGCATGTCCAGTTT
TTCTCGGGCTCCGGGCTCCTGCCCACGGCCACGGTCAAGGTGAAGACCCCGGACGGGGAGAGGCTCGCCACCCACACCGG
GGACGGTCCCGTGGATGCCGTGTACAAGGCCATCCAGGAGGCCATCGGCCTGAGGCCGGAGCTTGAGCTCTACCGGGTGG
AGGCCATCACGGGGAGCACCGAGGCCTTGGGCCAGGTCACGGTGCGCCTCCGGCTCGGCGAGCTCCAGGCCGTGGGGGTG
GGGGTCTCCCCGGACATCATTGAGGCGAGCGCCTTGGCCTTTTTGGACGCCGCCGGGAAGCTCGCCTCGGGCCGGGCCAC
CCGGCACCCGCCCTCCATTGAGGAGGTCCACCGTGGGGTCTAG

Upstream 100 bases:

>100_bases
GATGCCCTTCCTCAAGGCCAGGGTAATGGAGGAGGTAGGCTAAGGGCTTGGACGGGGCCCCTTCGGGGGCCCCTGTGCGC
TTTTAGGGAGGTGAAGGAAG

Downstream 100 bases:

>100_bases
GGGTGGGGGCTTTTAGGAAGCGTCCGTGGTGCGGATCCGTAGGGCCGGGCTAGAGGACCTCCCTGGGGTGGCTCGGGTCC
TGGTGGATACTTGGCGGGCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MEKERHIRIFDTTLRDGEQSPGVALSLDQKLEIAQALAQLNVDIIEAGFPVSGPMEFEAVRRIATEVKGPIIAALARTHT
LDIDQAAKALEKAEKPRIHVFTSASKVHLQYMLRKTEEEVLEMADRMVRYARRYVDDVEFSAQDVMRADWEFVKRLYEVA
IEAGATTINIPDTTGYGTPQEYGALIRRIRDEVVRGRDVVISTHTHDDLGMATANALAGVENGAGQIECTINGIGERAGN
CALEEVVMALYVRRDWYKAYTRINTREIYRVSRLVERYTGMPVPPNKAIVGDNAFAHESGIHQDGVIKHRATYEIMDAEL
IGRRPAVLVLGKHSGRAAFKKALEDLGYKDLSEEEVKKLFARFKEIAEKKGPLSAEELQALVESEREPTSHFFQLEHVQF
FSGSGLLPTATVKVKTPDGERLATHTGDGPVDAVYKAIQEAIGLRPELELYRVEAITGSTEALGQVTVRLRLGELQAVGV
GVSPDIIEASALAFLDAAGKLASGRATRHPPSIEEVHRGV

Sequences:

>Translated_520_residues
MEKERHIRIFDTTLRDGEQSPGVALSLDQKLEIAQALAQLNVDIIEAGFPVSGPMEFEAVRRIATEVKGPIIAALARTHT
LDIDQAAKALEKAEKPRIHVFTSASKVHLQYMLRKTEEEVLEMADRMVRYARRYVDDVEFSAQDVMRADWEFVKRLYEVA
IEAGATTINIPDTTGYGTPQEYGALIRRIRDEVVRGRDVVISTHTHDDLGMATANALAGVENGAGQIECTINGIGERAGN
CALEEVVMALYVRRDWYKAYTRINTREIYRVSRLVERYTGMPVPPNKAIVGDNAFAHESGIHQDGVIKHRATYEIMDAEL
IGRRPAVLVLGKHSGRAAFKKALEDLGYKDLSEEEVKKLFARFKEIAEKKGPLSAEELQALVESEREPTSHFFQLEHVQF
FSGSGLLPTATVKVKTPDGERLATHTGDGPVDAVYKAIQEAIGLRPELELYRVEAITGSTEALGQVTVRLRLGELQAVGV
GVSPDIIEASALAFLDAAGKLASGRATRHPPSIEEVHRGV
>Mature_520_residues
MEKERHIRIFDTTLRDGEQSPGVALSLDQKLEIAQALAQLNVDIIEAGFPVSGPMEFEAVRRIATEVKGPIIAALARTHT
LDIDQAAKALEKAEKPRIHVFTSASKVHLQYMLRKTEEEVLEMADRMVRYARRYVDDVEFSAQDVMRADWEFVKRLYEVA
IEAGATTINIPDTTGYGTPQEYGALIRRIRDEVVRGRDVVISTHTHDDLGMATANALAGVENGAGQIECTINGIGERAGN
CALEEVVMALYVRRDWYKAYTRINTREIYRVSRLVERYTGMPVPPNKAIVGDNAFAHESGIHQDGVIKHRATYEIMDAEL
IGRRPAVLVLGKHSGRAAFKKALEDLGYKDLSEEEVKKLFARFKEIAEKKGPLSAEELQALVESEREPTSHFFQLEHVQF
FSGSGLLPTATVKVKTPDGERLATHTGDGPVDAVYKAIQEAIGLRPELELYRVEAITGSTEALGQVTVRLRLGELQAVGV
GVSPDIIEASALAFLDAAGKLASGRATRHPPSIEEVHRGV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=493, Percent_Identity=46.8559837728195, Blast_Score=420, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320071, Length=386, Percent_Identity=26.6839378238342, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320019, Length=388, Percent_Identity=26.5463917525773, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=540, Percent_Identity=25, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6324682, Length=548, Percent_Identity=23.3576642335766, Blast_Score=111, Evalue=3e-25,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57441; Mature: 57441

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKERHIRIFDTTLRDGEQSPGVALSLDQKLEIAQALAQLNVDIIEAGFPVSGPMEFEAV
CCCCCEEEEEECHHCCCCCCCCEEEECHHHHHHHHHHHHCCHHEEECCCCCCCCCHHHHH
RRIATEVKGPIIAALARTHTLDIDQAAKALEKAEKPRIHVFTSASKVHLQYMLRKTEEEV
HHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
LEMADRMVRYARRYVDDVEFSAQDVMRADWEFVKRLYEVAIEAGATTINIPDTTGYGTPQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHH
EYGALIRRIRDEVVRGRDVVISTHTHDDLGMATANALAGVENGAGQIECTINGIGERAGN
HHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
CALEEVVMALYVRRDWYKAYTRINTREIYRVSRLVERYTGMPVPPNKAIVGDNAFAHESG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHCCC
IHQDGVIKHRATYEIMDAELIGRRPAVLVLGKHSGRAAFKKALEDLGYKDLSEEEVKKLF
CCCCCCEECCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHH
ARFKEIAEKKGPLSAEELQALVESEREPTSHFFQLEHVQFFSGSGLLPTATVKVKTPDGE
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHEEEHHEEEECCCCCCCEEEEEEECCCCC
RLATHTGDGPVDAVYKAIQEAIGLRPELELYRVEAITGSTEALGQVTVRLRLGELQAVGV
EEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHEEEEEEEECCEEEEEC
GVSPDIIEASALAFLDAAGKLASGRATRHPPSIEEVHRGV
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MEKERHIRIFDTTLRDGEQSPGVALSLDQKLEIAQALAQLNVDIIEAGFPVSGPMEFEAV
CCCCCEEEEEECHHCCCCCCCCEEEECHHHHHHHHHHHHCCHHEEECCCCCCCCCHHHHH
RRIATEVKGPIIAALARTHTLDIDQAAKALEKAEKPRIHVFTSASKVHLQYMLRKTEEEV
HHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
LEMADRMVRYARRYVDDVEFSAQDVMRADWEFVKRLYEVAIEAGATTINIPDTTGYGTPQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHH
EYGALIRRIRDEVVRGRDVVISTHTHDDLGMATANALAGVENGAGQIECTINGIGERAGN
HHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
CALEEVVMALYVRRDWYKAYTRINTREIYRVSRLVERYTGMPVPPNKAIVGDNAFAHESG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHCCC
IHQDGVIKHRATYEIMDAELIGRRPAVLVLGKHSGRAAFKKALEDLGYKDLSEEEVKKLF
CCCCCCEECCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHH
ARFKEIAEKKGPLSAEELQALVESEREPTSHFFQLEHVQFFSGSGLLPTATVKVKTPDGE
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHEEEHHEEEECCCCCCCEEEEEEECCCCC
RLATHTGDGPVDAVYKAIQEAIGLRPELELYRVEAITGSTEALGQVTVRLRLGELQAVGV
EEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHEEEEEEEECCEEEEEC
GVSPDIIEASALAFLDAAGKLASGRATRHPPSIEEVHRGV
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA