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The map label for this gene is polA

Identifier: 55981023

GI number: 55981023

Start: 996840

End: 999344

Strand: Direct

Name: polA

Synonym: TTHA1054

Alternate gene names: 55981023

Gene position: 996840-999344 (Clockwise)

Preceding gene: 55981022

Following gene: 55981025

Centisome position: 53.89

GC content: 67.86

Gene sequence:

>2505_bases
ATGGAGGCGATGCTTCCGCTCTTTGAACCCAAAGGCCGGGTCCTCCTGGTGGACGGCCACCACCTGGCCTACCGCACCTT
CTTCGCCCTGAAGGGCCTCACCACGAGCCGGGGCGAACCGGTGCAGGCGGTCTACGGCTTCGCCAAGAGCCTCCTCAAGG
CCCTGAAGGAGGACGGGTACAAGGCCGTCTTCGTGGTCTTTGACGCCAAGGCCCCCTCCTTCCGCCACGAGGCCTACGAG
GCCTACAAGGCGGGGAGGGCCCCGACCCCCGAGGACTTCCCCCGGCAGCTCGCCCTCATCAAGGAGCTGGTGGACCTCCT
GGGGTTTACCCGCCTCGAGGTCCCCGGCTACGAGGCGGACGACGTTCTCGCCACCCTGGCCAAGAAGGCGGAAAAGGAGG
GGTACGAGGTGCGCATCCTCACCGCCGACCGCGACCTCTACCAACTCGTCTCCGACCGCGTCGCCGTCCTCCACCCCGAG
GGCCACCTCATCACCCCGGAGTGGCTTTGGGAGAAGTACGGCCTCAGGCCGGAGCAGTGGGTGGACTTCCGCGCCCTCGT
GGGGGACCCCTCCGACAACCTCCCCGGGGTCAAGGGCATCGGGGAGAAGACCGCCCTCAAGCTCCTCAAGGAGTGGGGAA
GCCTGGAAAACCTCCTCAAGAACCTGGACCGGGTAAAGCCAGAAAACGTCCGGGAGAAGATCAAGGCCCACCTGGAAGAC
CTCAGGCTCTCCTTGGAGCTCTCCCGGGTGCGCACCGACCTCCCCCTGGAGGTGGACCTCGCCCAGGGGCGGGAGCCCGA
CCGGGAGGGGCTTAGGGCCTTCCTGGAGAGGCTGGAGTTCGGCAGCCTCCTCCACGAGTTCGGCCTCCTGGAGGCCCCCG
CCCCCCTGGAGGAGGCCCCCTGGCCCCCGCCGGAAGGGGCCTTCGTGGGCTTCGTCCTCTCCCGCCCCGAGCCCATGTGG
GCGGAGCTTAAAGCCCTGGCCGCCTGCAGGGACGGCCGGGTGCACCGGGCAGCAGACCCCTTGGCGGGGCTAAAGGACCT
CAAGGAGGTCCGGGGCCTCCTCGCCAAGGACCTCGCCGTCTTGGCCTCGAGGGAGGGGCTAGACCTCGTGCCCGGGGACG
ACCCCATGCTCCTCGCCTACCTCCTGGACCCCTCCAACACCACCCCCGAGGGGGTGGCGCGGCGCTACGGGGGGGAGTGG
ACGGAGGACGCCGCCCACCGGGCCCTCCTCTCGGAGAGGCTCCATCGGAACCTCCTTAAGCGCCTCGAGGGGGAGGAGAA
GCTCCTTTGGCTCTACCACGAGGTGGAAAAGCCCCTCTCCCGGGTCCTGGCCCACATGGAGGCCACCGGGGTACGGCTGG
ACGTGGCCTACCTTCAGGCCCTTTCCCTGGAGCTTGCGGAGGAGATCCGCCGCCTCGAGGAGGAGGTCTTCCGCTTGGCG
GGCCACCCCTTCAACCTCAACTCCCGGGACCAGCTGGAAAGGGTGCTCTTTGACGAGCTTAGGCTTCCCGCCTTGGGGAA
GACGCAAAAGACAGGCAAGCGCTCCACCAGCGCCGCGGTGCTGGAGGCCCTACGGGAGGCCCACCCCATCGTGGAGAAGA
TCCTCCAGCACCGGGAGCTCACCAAGCTCAAGAACACCTACGTGGACCCCCTCCCAAGCCTCGTCCACCCGAGGACGGGC
CGCCTCCACACCCGCTTCAACCAGACGGCCACGGCCACGGGGAGGCTTAGTAGCTCCGACCCCAACCTGCAGAACATCCC
CGTCCGCACCCCCTTGGGCCAGAGGATCCGCCGGGCCTTCGTGGCCGAGGCGGGTTGGGCGTTGGTGGCCCTGGACTATA
GCCAGATAGAGCTCCGCGTCCTCGCCCACCTCTCCGGGGACGAAAACCTGATCAGGGTCTTCCAGGAGGGGAAGGACATC
CACACCCAGACCGCAAGCTGGATGTTCGGCGTCCCCCCGGAGGCCGTGGACCCCCTGATGCGCCGGGCGGCCAAGACGGT
GAACTTCGGCGTCCTCTACGGCATGTCCGCCCATAGGCTCTCCCAGGAGCTTGCCATCCCCTACGAGGAGGCGGTGGCCT
TTATAGAGCGCTACTTCCAAAGCTTCCCCAAGGTGCGGGCCTGGATAGAAAAGACCCTGGAGGAGGGGAGGAAGCGGGGC
TACGTGGAAACCCTCTTCGGAAGAAGGCGCTACGTGCCCGACCTCAACGCCCGGGTGAAGAGCGTCAGGGAGGCCGCGGA
GCGCATGGCCTTCAACATGCCCGTCCAGGGCACCGCCGCCGACCTCATGAAGCTCGCCATGGTGAAGCTCTTCCCCCGCC
TCCGGGAGATGGGGGCCCGCATGCTCCTCCAGGTCCACGACGAGCTCCTCCTGGAGGCCCCCCAAGCGCGGGCCGAGGAG
GTGGCGGCTTTGGCCAAGGAGGCCATGGAGAAGGCCTATCCCCTCGCCGTGCCCCTGGAGGTGGAGGTGGGGATGGGGGA
GGACTGGCTTTCCGCCAAGGGTTAG

Upstream 100 bases:

>100_bases
GAGGGCGTGCGCTTCGCCCTCCTCCTCCCCAAGCCCGAGCGGGAAGGTTTCCTCAGGGCGCTCCTGGACGCCACCCGGGG
ACAGGTGGCCCTGGAGTAGC

Downstream 100 bases:

>100_bases
GGGGGCCCTGCCGTTTAGAGGAAGTTCAAGGGGTTGTCCCTCAGAAACGCCTCCAGGGGAACGCCCTCTGGGCCTACCAG
GAGGCCTTTAGCCCCAAAGG

Product: DNA polymerase I

Products: NA

Alternate protein names: Tth polymerase 1

Number of amino acids: Translated: 834; Mature: 834

Protein sequence:

>834_residues
MEAMLPLFEPKGRVLLVDGHHLAYRTFFALKGLTTSRGEPVQAVYGFAKSLLKALKEDGYKAVFVVFDAKAPSFRHEAYE
AYKAGRAPTPEDFPRQLALIKELVDLLGFTRLEVPGYEADDVLATLAKKAEKEGYEVRILTADRDLYQLVSDRVAVLHPE
GHLITPEWLWEKYGLRPEQWVDFRALVGDPSDNLPGVKGIGEKTALKLLKEWGSLENLLKNLDRVKPENVREKIKAHLED
LRLSLELSRVRTDLPLEVDLAQGREPDREGLRAFLERLEFGSLLHEFGLLEAPAPLEEAPWPPPEGAFVGFVLSRPEPMW
AELKALAACRDGRVHRAADPLAGLKDLKEVRGLLAKDLAVLASREGLDLVPGDDPMLLAYLLDPSNTTPEGVARRYGGEW
TEDAAHRALLSERLHRNLLKRLEGEEKLLWLYHEVEKPLSRVLAHMEATGVRLDVAYLQALSLELAEEIRRLEEEVFRLA
GHPFNLNSRDQLERVLFDELRLPALGKTQKTGKRSTSAAVLEALREAHPIVEKILQHRELTKLKNTYVDPLPSLVHPRTG
RLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAFVAEAGWALVALDYSQIELRVLAHLSGDENLIRVFQEGKDI
HTQTASWMFGVPPEAVDPLMRRAAKTVNFGVLYGMSAHRLSQELAIPYEEAVAFIERYFQSFPKVRAWIEKTLEEGRKRG
YVETLFGRRRYVPDLNARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLREMGARMLLQVHDELLLEAPQARAEE
VAALAKEAMEKAYPLAVPLEVEVGMGEDWLSAKG

Sequences:

>Translated_834_residues
MEAMLPLFEPKGRVLLVDGHHLAYRTFFALKGLTTSRGEPVQAVYGFAKSLLKALKEDGYKAVFVVFDAKAPSFRHEAYE
AYKAGRAPTPEDFPRQLALIKELVDLLGFTRLEVPGYEADDVLATLAKKAEKEGYEVRILTADRDLYQLVSDRVAVLHPE
GHLITPEWLWEKYGLRPEQWVDFRALVGDPSDNLPGVKGIGEKTALKLLKEWGSLENLLKNLDRVKPENVREKIKAHLED
LRLSLELSRVRTDLPLEVDLAQGREPDREGLRAFLERLEFGSLLHEFGLLEAPAPLEEAPWPPPEGAFVGFVLSRPEPMW
AELKALAACRDGRVHRAADPLAGLKDLKEVRGLLAKDLAVLASREGLDLVPGDDPMLLAYLLDPSNTTPEGVARRYGGEW
TEDAAHRALLSERLHRNLLKRLEGEEKLLWLYHEVEKPLSRVLAHMEATGVRLDVAYLQALSLELAEEIRRLEEEVFRLA
GHPFNLNSRDQLERVLFDELRLPALGKTQKTGKRSTSAAVLEALREAHPIVEKILQHRELTKLKNTYVDPLPSLVHPRTG
RLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAFVAEAGWALVALDYSQIELRVLAHLSGDENLIRVFQEGKDI
HTQTASWMFGVPPEAVDPLMRRAAKTVNFGVLYGMSAHRLSQELAIPYEEAVAFIERYFQSFPKVRAWIEKTLEEGRKRG
YVETLFGRRRYVPDLNARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLREMGARMLLQVHDELLLEAPQARAEE
VAALAKEAMEKAYPLAVPLEVEVGMGEDWLSAKG
>Mature_834_residues
MEAMLPLFEPKGRVLLVDGHHLAYRTFFALKGLTTSRGEPVQAVYGFAKSLLKALKEDGYKAVFVVFDAKAPSFRHEAYE
AYKAGRAPTPEDFPRQLALIKELVDLLGFTRLEVPGYEADDVLATLAKKAEKEGYEVRILTADRDLYQLVSDRVAVLHPE
GHLITPEWLWEKYGLRPEQWVDFRALVGDPSDNLPGVKGIGEKTALKLLKEWGSLENLLKNLDRVKPENVREKIKAHLED
LRLSLELSRVRTDLPLEVDLAQGREPDREGLRAFLERLEFGSLLHEFGLLEAPAPLEEAPWPPPEGAFVGFVLSRPEPMW
AELKALAACRDGRVHRAADPLAGLKDLKEVRGLLAKDLAVLASREGLDLVPGDDPMLLAYLLDPSNTTPEGVARRYGGEW
TEDAAHRALLSERLHRNLLKRLEGEEKLLWLYHEVEKPLSRVLAHMEATGVRLDVAYLQALSLELAEEIRRLEEEVFRLA
GHPFNLNSRDQLERVLFDELRLPALGKTQKTGKRSTSAAVLEALREAHPIVEKILQHRELTKLKNTYVDPLPSLVHPRTG
RLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAFVAEAGWALVALDYSQIELRVLAHLSGDENLIRVFQEGKDI
HTQTASWMFGVPPEAVDPLMRRAAKTVNFGVLYGMSAHRLSQELAIPYEEAVAFIERYFQSFPKVRAWIEKTLEEGRKRG
YVETLFGRRRYVPDLNARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLREMGARMLLQVHDELLLEAPQARAEE
VAALAKEAMEKAYPLAVPLEVEVGMGEDWLSAKG

Specific function: In Addition To Polymerase Activity, This DNA Polymerase Exhibits 3' To 5' And 5' To 3' Exonuclease Activity. It Is Able To Utilize Nicked Circular Duplex DNA As A Template And Can Unwind The Parental DNA Strand From Its Template. [C]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain

Homologues:

Organism=Homo sapiens, GI112420958, Length=509, Percent_Identity=35.1669941060904, Blast_Score=267, Evalue=4e-71,
Organism=Homo sapiens, GI139394648, Length=525, Percent_Identity=28.5714285714286, Blast_Score=191, Evalue=2e-48,
Organism=Escherichia coli, GI1790294, Length=431, Percent_Identity=45.4756380510441, Blast_Score=351, Evalue=1e-97,
Organism=Escherichia coli, GI226510970, Length=202, Percent_Identity=33.6633663366337, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI86563272, Length=407, Percent_Identity=31.4496314496315, Blast_Score=213, Evalue=4e-55,
Organism=Drosophila melanogaster, GI17933644, Length=423, Percent_Identity=30.4964539007092, Blast_Score=210, Evalue=4e-54,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DPO1T_THET8 (P52028)

Other databases:

- EMBL:   D28878
- EMBL:   AP008226
- RefSeq:   YP_144320.1
- ProteinModelPortal:   P52028
- SMR:   P52028
- STRING:   P52028
- GeneID:   3169068
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA1054
- NMPDR:   fig|300852.3.peg.1191
- eggNOG:   COG0749
- HOGENOM:   HBG284758
- OMA:   IPIRTEE
- ProtClustDB:   CLSK738582
- BioCyc:   TTHE300852:TTHA1054-MONOMER
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337
- InterPro:   IPR015361
- PRINTS:   PR00868
- SMART:   SM00475
- SMART:   SM00278
- SMART:   SM00279
- SMART:   SM00482
- TIGRFAMs:   TIGR00593

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A; PF09281 Taq-exonuc; SSF47807 5_3_exo_C; SSF53098 RNaseH_fold

EC number: =2.7.7.7

Molecular weight: Translated: 94008; Mature: 94008

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAMLPLFEPKGRVLLVDGHHLAYRTFFALKGLTTSRGEPVQAVYGFAKSLLKALKEDGY
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
KAVFVVFDAKAPSFRHEAYEAYKAGRAPTPEDFPRQLALIKELVDLLGFTRLEVPGYEAD
EEEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHH
DVLATLAKKAEKEGYEVRILTADRDLYQLVSDRVAVLHPEGHLITPEWLWEKYGLRPEQW
HHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCEEEECCCCCEECHHHHHHHHCCCHHHH
VDFRALVGDPSDNLPGVKGIGEKTALKLLKEWGSLENLLKNLDRVKPENVREKIKAHLED
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LRLSLELSRVRTDLPLEVDLAQGREPDREGLRAFLERLEFGSLLHEFGLLEAPAPLEEAP
HHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
WPPPEGAFVGFVLSRPEPMWAELKALAACRDGRVHRAADPLAGLKDLKEVRGLLAKDLAV
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
LASREGLDLVPGDDPMLLAYLLDPSNTTPEGVARRYGGEWTEDAAHRALLSERLHRNLLK
HHCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RLEGEEKLLWLYHEVEKPLSRVLAHMEATGVRLDVAYLQALSLELAEEIRRLEEEVFRLA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHC
GHPFNLNSRDQLERVLFDELRLPALGKTQKTGKRSTSAAVLEALREAHPIVEKILQHREL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TKLKNTYVDPLPSLVHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAF
HHHHHHHCCCCHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VAEAGWALVALDYSQIELRVLAHLSGDENLIRVFQEGKDIHTQTASWMFGVPPEAVDPLM
HHHCCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHCCCEECCCHHHHHHHH
RRAAKTVNFGVLYGMSAHRLSQELAIPYEEAVAFIERYFQSFPKVRAWIEKTLEEGRKRG
HHHHHHHCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YVETLFGRRRYVPDLNARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLREMGAR
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MLLQVHDELLLEAPQARAEEVAALAKEAMEKAYPLAVPLEVEVGMGEDWLSAKG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCC
>Mature Secondary Structure
MEAMLPLFEPKGRVLLVDGHHLAYRTFFALKGLTTSRGEPVQAVYGFAKSLLKALKEDGY
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
KAVFVVFDAKAPSFRHEAYEAYKAGRAPTPEDFPRQLALIKELVDLLGFTRLEVPGYEAD
EEEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHH
DVLATLAKKAEKEGYEVRILTADRDLYQLVSDRVAVLHPEGHLITPEWLWEKYGLRPEQW
HHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCEEEECCCCCEECHHHHHHHHCCCHHHH
VDFRALVGDPSDNLPGVKGIGEKTALKLLKEWGSLENLLKNLDRVKPENVREKIKAHLED
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LRLSLELSRVRTDLPLEVDLAQGREPDREGLRAFLERLEFGSLLHEFGLLEAPAPLEEAP
HHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
WPPPEGAFVGFVLSRPEPMWAELKALAACRDGRVHRAADPLAGLKDLKEVRGLLAKDLAV
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
LASREGLDLVPGDDPMLLAYLLDPSNTTPEGVARRYGGEWTEDAAHRALLSERLHRNLLK
HHCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RLEGEEKLLWLYHEVEKPLSRVLAHMEATGVRLDVAYLQALSLELAEEIRRLEEEVFRLA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHC
GHPFNLNSRDQLERVLFDELRLPALGKTQKTGKRSTSAAVLEALREAHPIVEKILQHREL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TKLKNTYVDPLPSLVHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAF
HHHHHHHCCCCHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VAEAGWALVALDYSQIELRVLAHLSGDENLIRVFQEGKDIHTQTASWMFGVPPEAVDPLM
HHHCCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHCCCEECCCHHHHHHHH
RRAAKTVNFGVLYGMSAHRLSQELAIPYEEAVAFIERYFQSFPKVRAWIEKTLEEGRKRG
HHHHHHHCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YVETLFGRRRYVPDLNARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLREMGAR
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MLLQVHDELLLEAPQARAEEVAALAKEAMEKAYPLAVPLEVEVGMGEDWLSAKG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA