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The map label for this gene is thiC [H]

Identifier: 55980647

GI number: 55980647

Start: 642770

End: 644077

Strand: Direct

Name: thiC [H]

Synonym: TTHA0678

Alternate gene names: 55980647

Gene position: 642770-644077 (Clockwise)

Preceding gene: 55980646

Following gene: 55980648

Centisome position: 34.75

GC content: 66.82

Gene sequence:

>1308_bases
ATGACGCAGCTTGAGGCGGCAAGGAAAGGGAAGATCACCGAGGAGATGGCCTACGTGGCCGAGAAGGAAGGGGTTTCCCC
CGAGTTCGTGCGGGAAGGGGTGGCGGCGGGCCGGATCGTCATCCCCAGGAACCCGAACCACAAGACCCTCACCGACTTCA
AGGGGATCGGGGAGGGGCTTTCCGTCAAGGTGAACGCCAACCTGGGCACCTCCTACGACTACGTGGACGTGGAGGAGGAG
GTGGAGAAGGCCAGGGTGGCCATCCAGTACGGGGCGGACACCGTTATGGACCTCTCCACCGGGGGAGACCTCAAGGCGAT
CCGCAAGCGGATCCTGGAGGTGGCCACCGTCCCCTTGGGCACCGTGCCCATCTACGAGGCGGAGTTTCGGGCGGCCAGGC
GCAAGAACTTCTTTGACATGTCGGCGGACGAGCTCTTCCAGGTGATTGAGGAGCACGGCAAGGAGGGGGTGGACTACATC
ACCGTGCACGTGGGGGTGACCCTCAAGAACCTCGAGGTCTACCGGAATAGCCCCCGCACCACGGGCATCGTGAGCCGGGG
CGGGGGGCTCATGGCCGCCTGGATGCTCCACCGCGGCGAGGAAAACCCCCTCTACGCCCGCTTTGACGACCTTTTGGACA
TCGCCCGCACCTACGACATGACCCTCTCCCTGGGGGACGGCCTCCGCCCAGGCTCCCTCGCCGACAGCACCGACCGGGCC
CAGATCGCAGAGCTCCTCACCATCGGGGAGCTCGTGGAAAGGGCCAGAAGGGCCGGGGTCCAGGCCATGGTGGAAGGCCC
CGGGCACATCCCCTTGAACGAGGTGGCGGCCAACGTCCAGATCCAGAAGAAGCTCACGGGGCACGCGCCCTTCTACATCC
TGGGGATGCTTCCCGTGGACACCGCCGCCGGCTTTGACCACATCGCCGGGGCCATCGGCGGGGCCTTGGCGGGATGGTGG
GGTGCGGACATGCTCTGCTACCTGACCCCGGCGGAGCACCTGGGCCTGCCCACCCCTGAGCACGTGAAGCAAGGGGTGAT
CGCCTTTAAGATCGCCGCCCACGCCGCCGACGTGGCCCGGGGGAACAAAAGGGCCCTGGAGCGGAACCGGAGGATGTCCG
AGGCCCGCTACCGGTTGGATTGGGAGGGCCAGTTCGCCCTCGCCCTCTTCCCCGAGGAGGCGCGGCGCCTCAAGGAGGAG
CGGGGCTCCAAGACCAAGGCCTGCAGCATGTGCGGCCCCTTCTGCCCCATGAACCTGGTGGAGGCGGTGCTTAAGGGGAA
GGGGCGGATGGAGCTTCCGGTAGCCTGA

Upstream 100 bases:

>100_bases
GTGGAGGAAGGCGTGTACGCGGCGGTGGGGCACGGGAGGAACGGCGTCCTCCTCGCCCCTTGGACGGCAAAGAGGCTTTT
AAAGCTTTTGGGGGTGGAGG

Downstream 100 bases:

>100_bases
GGCCATGGACGCCCTCGCCCGTCTGGAACGCCTGCCCTTGGGCAGGCCCCACTACCGCCTCCTCCTGCTTCTCGGGTTCG
GCTGGGCCCTGGACGCCATG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTQLEAARKGKITEEMAYVAEKEGVSPEFVREGVAAGRIVIPRNPNHKTLTDFKGIGEGLSVKVNANLGTSYDYVDVEEE
VEKARVAIQYGADTVMDLSTGGDLKAIRKRILEVATVPLGTVPIYEAEFRAARRKNFFDMSADELFQVIEEHGKEGVDYI
TVHVGVTLKNLEVYRNSPRTTGIVSRGGGLMAAWMLHRGEENPLYARFDDLLDIARTYDMTLSLGDGLRPGSLADSTDRA
QIAELLTIGELVERARRAGVQAMVEGPGHIPLNEVAANVQIQKKLTGHAPFYILGMLPVDTAAGFDHIAGAIGGALAGWW
GADMLCYLTPAEHLGLPTPEHVKQGVIAFKIAAHAADVARGNKRALERNRRMSEARYRLDWEGQFALALFPEEARRLKEE
RGSKTKACSMCGPFCPMNLVEAVLKGKGRMELPVA

Sequences:

>Translated_435_residues
MTQLEAARKGKITEEMAYVAEKEGVSPEFVREGVAAGRIVIPRNPNHKTLTDFKGIGEGLSVKVNANLGTSYDYVDVEEE
VEKARVAIQYGADTVMDLSTGGDLKAIRKRILEVATVPLGTVPIYEAEFRAARRKNFFDMSADELFQVIEEHGKEGVDYI
TVHVGVTLKNLEVYRNSPRTTGIVSRGGGLMAAWMLHRGEENPLYARFDDLLDIARTYDMTLSLGDGLRPGSLADSTDRA
QIAELLTIGELVERARRAGVQAMVEGPGHIPLNEVAANVQIQKKLTGHAPFYILGMLPVDTAAGFDHIAGAIGGALAGWW
GADMLCYLTPAEHLGLPTPEHVKQGVIAFKIAAHAADVARGNKRALERNRRMSEARYRLDWEGQFALALFPEEARRLKEE
RGSKTKACSMCGPFCPMNLVEAVLKGKGRMELPVA
>Mature_434_residues
TQLEAARKGKITEEMAYVAEKEGVSPEFVREGVAAGRIVIPRNPNHKTLTDFKGIGEGLSVKVNANLGTSYDYVDVEEEV
EKARVAIQYGADTVMDLSTGGDLKAIRKRILEVATVPLGTVPIYEAEFRAARRKNFFDMSADELFQVIEEHGKEGVDYIT
VHVGVTLKNLEVYRNSPRTTGIVSRGGGLMAAWMLHRGEENPLYARFDDLLDIARTYDMTLSLGDGLRPGSLADSTDRAQ
IAELLTIGELVERARRAGVQAMVEGPGHIPLNEVAANVQIQKKLTGHAPFYILGMLPVDTAAGFDHIAGAIGGALAGWWG
ADMLCYLTPAEHLGLPTPEHVKQGVIAFKIAAHAADVARGNKRALERNRRMSEARYRLDWEGQFALALFPEEARRLKEER
GSKTKACSMCGPFCPMNLVEAVLKGKGRMELPVA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=456, Percent_Identity=44.9561403508772, Blast_Score=370, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 47586; Mature: 47455

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLEAARKGKITEEMAYVAEKEGVSPEFVREGVAAGRIVIPRNPNHKTLTDFKGIGEGL
CCCCCCHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCC
SVKVNANLGTSYDYVDVEEEVEKARVAIQYGADTVMDLSTGGDLKAIRKRILEVATVPLG
EEEEECCCCCCCCEECHHHHHHHHHEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCC
TVPIYEAEFRAARRKNFFDMSADELFQVIEEHGKEGVDYITVHVGVTLKNLEVYRNSPRT
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEHHCCCCCCC
TGIVSRGGGLMAAWMLHRGEENPLYARFDDLLDIARTYDMTLSLGDGLRPGSLADSTDRA
CEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCHHH
QIAELLTIGELVERARRAGVQAMVEGPGHIPLNEVAANVQIQKKLTGHAPFYILGMLPVD
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCEEEEEHHCCCCCEEEEEEECCC
TAAGFDHIAGAIGGALAGWWGADMLCYLTPAEHLGLPTPEHVKQGVIAFKIAAHAADVAR
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHCCHHEEEHHHHHHHHHC
GNKRALERNRRMSEARYRLDWEGQFALALFPEEARRLKEERGSKTKACSMCGPFCPMNLV
CCHHHHHHHHHHHHHHEEECCCCCEEEEECCHHHHHHHHHHCCCCCHHHHCCCCCHHHHH
EAVLKGKGRMELPVA
HHHHCCCCCEECCCC
>Mature Secondary Structure 
TQLEAARKGKITEEMAYVAEKEGVSPEFVREGVAAGRIVIPRNPNHKTLTDFKGIGEGL
CCCCCHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCC
SVKVNANLGTSYDYVDVEEEVEKARVAIQYGADTVMDLSTGGDLKAIRKRILEVATVPLG
EEEEECCCCCCCCEECHHHHHHHHHEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCC
TVPIYEAEFRAARRKNFFDMSADELFQVIEEHGKEGVDYITVHVGVTLKNLEVYRNSPRT
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEHHCCCCCCC
TGIVSRGGGLMAAWMLHRGEENPLYARFDDLLDIARTYDMTLSLGDGLRPGSLADSTDRA
CEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCHHH
QIAELLTIGELVERARRAGVQAMVEGPGHIPLNEVAANVQIQKKLTGHAPFYILGMLPVD
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCEEEEEHHCCCCCEEEEEEECCC
TAAGFDHIAGAIGGALAGWWGADMLCYLTPAEHLGLPTPEHVKQGVIAFKIAAHAADVAR
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHCCHHEEEHHHHHHHHHC
GNKRALERNRRMSEARYRLDWEGQFALALFPEEARRLKEERGSKTKACSMCGPFCPMNLV
CCHHHHHHHHHHHHHHEEECCCCCEEEEECCHHHHHHHHHHCCCCCHHHHCCCCCHHHHH
EAVLKGKGRMELPVA
HHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA