Click here to switch to the map view.

The map label for this gene is ilvE [C]

Identifier: 55980590

GI number: 55980590

Start: 593608

End: 594348

Strand: Direct

Name: ilvE [C]

Synonym: TTHA0621

Alternate gene names: 55980590

Gene position: 593608-594348 (Clockwise)

Preceding gene: 55980589

Following gene: 55980591

Centisome position: 32.09

GC content: 72.87

Gene sequence:

>741_bases
ATGAGGCTTTTAAACGGCACGCCCCTCGCCCTCGCCCTCCCCGAGGCCTTCCTCTACCACGGGGCGAGCGTCTTCACCAC
GCTCCGGGCGGAAGGGGGAAGGCCCCTTTGGCTTGAGGAGCACCTGGCCCGCCTCCGCCGCCACGCCCTGGCCCTGGGGC
TTTCCTACCCGGGGGACGAGGCCTTCCTCGAGGACCTCGAGGCCCTCCTCCGGGCCTTTCCCAAGGCGCCCTGCCTCCGC
CTCCGCTTCACCGTGGGGGAGGGGGTGCGGCTCTCCGAGGCGAGGCCCTACGCCCCCCTGCCCCTTTCCCTCTACCGGGA
GGGGGTCCGGGTCCGCCTCACGGGCTACCGGGTCCACCCCGACCTCGCCCGCTACAAGACGGGGAACTACCTCCCCTACC
GCCTGGCCCTGGAGGAGGCCCGGAAGGAGGGGGCCTTTGAGGGCCTCCTCCTGGACGCCTTCGGCCACGTGGTGGACGGG
AGCCGCACGAGCCCCCTCCTCTTCCGGGAGGGGACGCTCTACCTCCTGGAGGGGGGCCTCGAGGGGATCACCCGGGAGAA
GGTGGCGGAGGCCGCCCGGGGCCTGGGCCTCCGGGTGGAGCGGGGCCTCTTTCGCCCCGAGGGGCTCCGGGGCCACCTCC
TCCTCGCGGGAAGCGGGGTGGGCCTCCTCCCCGTGAGGCCGCCCCCTCCCGAGCTCCTTCCCCTCATAGAGCGCTTCCTT
CCCGCCTGCTATACTGAGTAG

Upstream 100 bases:

>100_bases
CCATCTGGGACGGGAGGCGGGCCTACGGGGTCCAGTTCCACCCGGAAAGCCTCCTTTCCCCTTACGGCATGCTCCTTCTC
GCCCGGTTCCTGGAGGTGGG

Downstream 100 bases:

>100_bases
CGGTACCGCCAGCCCAAAGGCCTTGGGCTGGTGCCCCTTTTTGCACGGGAGGAGTCCATGAAGAAGCCCGTCTACCTGAC
CCCGGAAGGCTACCGGCGCC

Product: 4-amino-4-deoxychorismate lyase

Products: 2-Oxoglutarate; L-Leucine; 3-Methyl-2-oxobutanoate; L-Glutamate; (S)-3-Methyl-2-oxopentanoate [C]

Alternate protein names: None

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRLLNGTPLALALPEAFLYHGASVFTTLRAEGGRPLWLEEHLARLRRHALALGLSYPGDEAFLEDLEALLRAFPKAPCLR
LRFTVGEGVRLSEARPYAPLPLSLYREGVRVRLTGYRVHPDLARYKTGNYLPYRLALEEARKEGAFEGLLLDAFGHVVDG
SRTSPLLFREGTLYLLEGGLEGITREKVAEAARGLGLRVERGLFRPEGLRGHLLLAGSGVGLLPVRPPPPELLPLIERFL
PACYTE

Sequences:

>Translated_246_residues
MRLLNGTPLALALPEAFLYHGASVFTTLRAEGGRPLWLEEHLARLRRHALALGLSYPGDEAFLEDLEALLRAFPKAPCLR
LRFTVGEGVRLSEARPYAPLPLSLYREGVRVRLTGYRVHPDLARYKTGNYLPYRLALEEARKEGAFEGLLLDAFGHVVDG
SRTSPLLFREGTLYLLEGGLEGITREKVAEAARGLGLRVERGLFRPEGLRGHLLLAGSGVGLLPVRPPPPELLPLIERFL
PACYTE
>Mature_246_residues
MRLLNGTPLALALPEAFLYHGASVFTTLRAEGGRPLWLEEHLARLRRHALALGLSYPGDEAFLEDLEALLRAFPKAPCLR
LRFTVGEGVRLSEARPYAPLPLSLYREGVRVRLTGYRVHPDLARYKTGNYLPYRLALEEARKEGAFEGLLLDAFGHVVDG
SRTSPLLFREGTLYLLEGGLEGITREKVAEAARGLGLRVERGLFRPEGLRGHLLLAGSGVGLLPVRPPPPELLPLIERFL
PACYTE

Specific function: Acts On Leucine, Isoleucine And Valine. [C]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.6.1.42 [C]

Molecular weight: Translated: 27253; Mature: 27253

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLNGTPLALALPEAFLYHGASVFTTLRAEGGRPLWLEEHLARLRRHALALGLSYPGDE
CCCCCCCCCEEEHHHHHHHCCHHHEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCHH
AFLEDLEALLRAFPKAPCLRLRFTVGEGVRLSEARPYAPLPLSLYREGVRVRLTGYRVHP
HHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCCCCCCCHHHHHCCEEEEEEEEEECC
DLARYKTGNYLPYRLALEEARKEGAFEGLLLDAFGHVVDGSRTSPLLFREGTLYLLEGGL
HHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCC
EGITREKVAEAARGLGLRVERGLFRPEGLRGHLLLAGSGVGLLPVRPPPPELLPLIERFL
CCCHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEECCCEEEECCCCCHHHHHHHHHHH
PACYTE
HHHCCC
>Mature Secondary Structure
MRLLNGTPLALALPEAFLYHGASVFTTLRAEGGRPLWLEEHLARLRRHALALGLSYPGDE
CCCCCCCCCEEEHHHHHHHCCHHHEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCHH
AFLEDLEALLRAFPKAPCLRLRFTVGEGVRLSEARPYAPLPLSLYREGVRVRLTGYRVHP
HHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCCCCCCCHHHHHCCEEEEEEEEEECC
DLARYKTGNYLPYRLALEEARKEGAFEGLLLDAFGHVVDGSRTSPLLFREGTLYLLEGGL
HHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCC
EGITREKVAEAARGLGLRVERGLFRPEGLRGHLLLAGSGVGLLPVRPPPPELLPLIERFL
CCCHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEECCCEEEECCCCCHHHHHHHHHHH
PACYTE
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.2 {3-methyl-2-oxopentanoate}} 19 {L-methionine}} 2.2 {L-leucine}} 0.56 {L-leucine}} 3.13 {L-valine}} 2.7 {L-valine}} 1.26 {L-valine}} 0.97 {2-oxoglutarate}} 0.42 {L-isoleucine}} [C]

Substrates: 4-Methyl-2-oxopentanoate; L-Glutamate; 2-Oxoglutarate; L-Valine; L-Isoleucine [C]

Specific reaction: 4-Methyl-2-oxopentanoate + L-Glutamate --> 2-Oxoglutarate + L-Leucine 2-Oxoglutarate + L-Valine <==> 3-Methyl-2-oxobutanoate + L-Glutamate 2-Oxoglutarate + L-Isoleucine <==> (S)-3-Methyl-2-oxopentanoate + L-Glutamate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA