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The map label for this gene is ask

Identifier: 55980503

GI number: 55980503

Start: 496759

End: 497976

Strand: Direct

Name: ask

Synonym: TTHA0534

Alternate gene names: 55980503

Gene position: 496759-497976 (Clockwise)

Preceding gene: 55980499

Following gene: 55980504

Centisome position: 26.86

GC content: 67.98

Gene sequence:

>1218_bases
GTGGCCCTGGTGGTTCAGAAGTACGGCGGCACCTCCGTGGGCGACCTGGAGCGCATCCACAAGGTGGCCCAGCGCATCGC
CCACTACCGCGAGAAGGGGCATCGGCTTGCGGTGGTGGTCTCGGCCATGGGGCACACCACCGACGAGCTCATCGCCTTGG
CCAAGCGGGTGAACCCGAGACCTCCCTTTCGCGAGCTGGACCTCCTCACCACCACCGGGGAGCAGGTCTCCGTGGCCCTC
CTCTCCATGCAGCTTTGGGCCATGGGGATCCCCGCCAAGGGCTTCGTCCAGCACCAGATCGGCATCACCACCGACGGGCG
CTACGGGGACGCCCGGATCCTCGAGGTGAACCCGGCCCGCATCCGGGAGGCCCTGGAGCAGGGCTTTGTGGCGGTGATCG
CGGGCTTCATGGGCACCACCCCGGAAGGGGAGATCACCACCTTGGGCCGGGGAGGGTCGGACACCACCGCCGTGGCCATC
GCCGCCGCCCTCGGGGCCAAGGAGTGCGAGATCTACACGGACACGGAGGGGGTCTACACCACGGACCCCCACCTGATCCC
CGAGGCGAGGAAGCTTTCCGTCATCGGCTACGACCAGATGCTGGAGATGGCCGCTTTGGGGGCCAGGGTCCTCCACCCCC
GGGCGGTCTACTACGCCAAGCGCTACGGGGTGGTCCTCCACGTGCGCTCTAGCTTCTCCTACAACCCCGGTACCCTGGTG
AAGGAGGTCGCCATGGAGATGGACAAGGCGGTGACGGGCGTGGCCTTGGACCTGGACCACGCCCAGATCGGGCTTATCGG
CATCCCGGACCAGCCGGGCATCGCGGCCAAGGTCTTCCAGGCCCTGGCCGAGCGGGGCATCGCCGTGGACATGATCATCC
AGGGGGTTCCCGGCCACGACCCCTCGAGGCAGCAGATGGCCTTCACGGTGAAGAAGGACTTCGCCCAGGAGGCCCTGGAG
GCTTTGGAGCCCGTCCTCGCCGAGATCGGGGGCGAGGCCATCTTGAGGCCGGACATCGCCAAGGTCTCCATCGTGGGCGT
GGGCCTCGCCTCCACCCCGGAGGTCCCCGCCAAGATGTTCCAGGCGGTGGCCTCCACCGGGGCCAACATTGAGATGATCG
CCACCAGCGAGGTGCGCATCTCCGTGATCATCCCCGCCGAGTACGCCGAGGCGGCGCTTAGGGCCGTCCACCAGGCTTTT
GAGCTGGACAAGGCCTGA

Upstream 100 bases:

>100_bases
TCAGGCTCACGTCCTCCCAGGATAAGGGCCTTGGGCGGAGGGGGCAAATGTGCTAACATTCTCTTTCAAACCGCCCGGGG
GGGCGGGGAAGGAGGGAAGC

Downstream 100 bases:

>100_bases
TGGAGCGCCTCCACCTTTCCTGGGAGGACCTCCTCCGGCTGGTCCGCCGCCTGGCGGAGGCCCTTTCTGGGGAGCCTTTT
GACCTCGTCCTGGGGGTGGC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase; ASK

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MALVVQKYGGTSVGDLERIHKVAQRIAHYREKGHRLAVVVSAMGHTTDELIALAKRVNPRPPFRELDLLTTTGEQVSVAL
LSMQLWAMGIPAKGFVQHQIGITTDGRYGDARILEVNPARIREALEQGFVAVIAGFMGTTPEGEITTLGRGGSDTTAVAI
AAALGAKECEIYTDTEGVYTTDPHLIPEARKLSVIGYDQMLEMAALGARVLHPRAVYYAKRYGVVLHVRSSFSYNPGTLV
KEVAMEMDKAVTGVALDLDHAQIGLIGIPDQPGIAAKVFQALAERGIAVDMIIQGVPGHDPSRQQMAFTVKKDFAQEALE
ALEPVLAEIGGEAILRPDIAKVSIVGVGLASTPEVPAKMFQAVASTGANIEMIATSEVRISVIIPAEYAEAALRAVHQAF
ELDKA

Sequences:

>Translated_405_residues
MALVVQKYGGTSVGDLERIHKVAQRIAHYREKGHRLAVVVSAMGHTTDELIALAKRVNPRPPFRELDLLTTTGEQVSVAL
LSMQLWAMGIPAKGFVQHQIGITTDGRYGDARILEVNPARIREALEQGFVAVIAGFMGTTPEGEITTLGRGGSDTTAVAI
AAALGAKECEIYTDTEGVYTTDPHLIPEARKLSVIGYDQMLEMAALGARVLHPRAVYYAKRYGVVLHVRSSFSYNPGTLV
KEVAMEMDKAVTGVALDLDHAQIGLIGIPDQPGIAAKVFQALAERGIAVDMIIQGVPGHDPSRQQMAFTVKKDFAQEALE
ALEPVLAEIGGEAILRPDIAKVSIVGVGLASTPEVPAKMFQAVASTGANIEMIATSEVRISVIIPAEYAEAALRAVHQAF
ELDKA
>Mature_404_residues
ALVVQKYGGTSVGDLERIHKVAQRIAHYREKGHRLAVVVSAMGHTTDELIALAKRVNPRPPFRELDLLTTTGEQVSVALL
SMQLWAMGIPAKGFVQHQIGITTDGRYGDARILEVNPARIREALEQGFVAVIAGFMGTTPEGEITTLGRGGSDTTAVAIA
AALGAKECEIYTDTEGVYTTDPHLIPEARKLSVIGYDQMLEMAALGARVLHPRAVYYAKRYGVVLHVRSSFSYNPGTLVK
EVAMEMDKAVTGVALDLDHAQIGLIGIPDQPGIAAKVFQALAERGIAVDMIIQGVPGHDPSRQQMAFTVKKDFAQEALEA
LEPVLAEIGGEAILRPDIAKVSIVGVGLASTPEVPAKMFQAVASTGANIEMIATSEVRISVIIPAEYAEAALRAVHQAFE
LDKA

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains

Homologues:

Organism=Escherichia coli, GI1786183, Length=285, Percent_Identity=32.280701754386, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790455, Length=461, Percent_Identity=27.9826464208243, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790376, Length=278, Percent_Identity=28.4172661870504, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320893, Length=370, Percent_Identity=23.5135135135135, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AK_THET2 (P61488)

Other databases:

- EMBL:   AB013131
- EMBL:   AB013131
- EMBL:   AE017221
- RefSeq:   YP_004141.1
- ProteinModelPortal:   P61488
- SMR:   P61488
- STRING:   P61488
- GeneID:   2775578
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0527
- HOGENOM:   HBG724395
- OMA:   NPSKREM
- PhylomeDB:   P61488
- ProtClustDB:   PRK06635
- BioCyc:   TTHE262724:TT_C0166-MONOMER
- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042
- Gene3D:   G3DSA:3.40.1160.10
- TIGRFAMs:   TIGR00656
- TIGRFAMs:   TIGR00657

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; SSF53633 Aa_kinase

EC number: =2.7.2.4

Molecular weight: Translated: 43334; Mature: 43202

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVVQKYGGTSVGDLERIHKVAQRIAHYREKGHRLAVVVSAMGHTTDELIALAKRVNPR
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCHHHHHHHHHHCCCC
PPFRELDLLTTTGEQVSVALLSMQLWAMGIPAKGFVQHQIGITTDGRYGDARILEVNPAR
CCHHHCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEECHHH
IREALEQGFVAVIAGFMGTTPEGEITTLGRGGSDTTAVAIAAALGAKECEIYTDTEGVYT
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCEEE
TDPHLIPEARKLSVIGYDQMLEMAALGARVLHPRAVYYAKRYGVVLHVRSSFSYNPGTLV
CCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCHHH
KEVAMEMDKAVTGVALDLDHAQIGLIGIPDQPGIAAKVFQALAERGIAVDMIIQGVPGHD
HHHHHHHHHHHHEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
PSRQQMAFTVKKDFAQEALEALEPVLAEIGGEAILRPDIAKVSIVGVGLASTPEVPAKMF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCCCCHHHHHH
QAVASTGANIEMIATSEVRISVIIPAEYAEAALRAVHQAFELDKA
HHHHHCCCCEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALVVQKYGGTSVGDLERIHKVAQRIAHYREKGHRLAVVVSAMGHTTDELIALAKRVNPR
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCHHHHHHHHHHCCCC
PPFRELDLLTTTGEQVSVALLSMQLWAMGIPAKGFVQHQIGITTDGRYGDARILEVNPAR
CCHHHCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEECHHH
IREALEQGFVAVIAGFMGTTPEGEITTLGRGGSDTTAVAIAAALGAKECEIYTDTEGVYT
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCEEE
TDPHLIPEARKLSVIGYDQMLEMAALGARVLHPRAVYYAKRYGVVLHVRSSFSYNPGTLV
CCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCHHH
KEVAMEMDKAVTGVALDLDHAQIGLIGIPDQPGIAAKVFQALAERGIAVDMIIQGVPGHD
HHHHHHHHHHHHEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
PSRQQMAFTVKKDFAQEALEALEPVLAEIGGEAILRPDIAKVSIVGVGLASTPEVPAKMF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCCCCHHHHHH
QAVASTGANIEMIATSEVRISVIIPAEYAEAALRAVHQAFELDKA
HHHHHCCCCEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10074061