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The map label for this gene is glcF [H]

Identifier: 55980472

GI number: 55980472

Start: 466849

End: 468105

Strand: Reverse

Name: glcF [H]

Synonym: TTHA0503

Alternate gene names: 55980472

Gene position: 468105-466849 (Counterclockwise)

Preceding gene: 55980473

Following gene: 55980471

Centisome position: 25.31

GC content: 69.77

Gene sequence:

>1257_bases
ATGCAACACCAAATCCCCGTAGAAAAGCTGGGCAAGGAGGGGGAGGTGATGGCCCACGCCGTTGAGGCCTGCGTCCACTG
CGGCTTCTGCCTCCCCACCTGCCCCACCTACGTGGTCCTCCAGGAGGAGATGGACTCCCCCAGGGGACGGATCTTCCTCA
TGAAGGAGGTCTTGGAGGGGAACCTGGGGTTGGAAGAAGCCCTCCCCTACCTGGACCGCTGCCTCGCCTGCCAGGCCTGC
GTCACCGCCTGCCCAAGCGGCGTCCCCTACGGCGAGCTCATCGCCACCTTCCGCCTGTGGAGCGAGGAAAAGCGGCACCG
CTTCCCCTTGGAAAGGGTCTTCCGCCTGGGCCTCCTCCGCACCCTCCCCTACCCGGAGCGCTTCCGGCCCCTGGCGAAGC
TCGGCGCGGGCCTGAAGCCCCTCCTCAGGCCCCTTCCCCTTCCCAAGCCCCTGAAGGCCCCCCTCGCCCTCCTCCCGGAC
CGCCTTCCCGAGGAGGCCCCCTACGGGGAGGTCTACCCCGCCAAGGGGGAGAGGCGGGCGCGGGTGGGGCTCCTTCTGGG
GTGCGCCCAGAGGGTCTTGAGGCCTTCCATCAACCAGGCCACCATCCGGGTCCTCCAGGAAAACGGGGTGGAGGTGGTGG
CGGTGCCGGAGCAGGCCTGCTGCGGCGCCCTCAACCTCCACGCCGGGGACAAGGAGGGGGCGCGGGCCCTCGCCCGGAAG
AACCTGAAGGCCTTCCGGGACGTGGACTACGTGGTCACCAACGCCGCCGGGTGCGGCTCGGGGATGAAGGAGTACCCCCT
CCTCTTCCGGGGGGAGCCCGAGGAGGAGGAGGCCCGGGCCCTCGCCGCCAAGGTGAAGGACTTGAGCGTCCTCCTGGACG
AGCTGGGCTTCACCCCGCCCCCACCCCCCAGGCGCCCCTTGCGGGTGGCCTACCACGACGCCTGCCACCTGGCCCACGCC
CAGGGGGTGAGGGAGGCCCCAAGAAGGCTTCTCCAGGCGGCGGGCCTCGAGGTCTTGGAACCCAGGGAGTGGGAGCTCTG
CTGCGGCTCCGCCGGGACCTACAACCTCTTCCAGCCGGAGATCGCCGAGGCCCTGGGAAGGAGGAAGGCGGAAAACCTCA
AGGCCACGGGGGCGGACCTCGTGGCCACGGGGAACATCGGCTGCCTCACCCAGATCCAGGCCTACCTGGACCTCCCCGTG
CTTCACACCGCCGAGCTTCTGGCCCTCCTCTACGAGGGAAAGGACCCTCTGGCTTGA

Upstream 100 bases:

>100_bases
GGGGCGGAAGCGCCCCTCTTCCCCGAGCCCCAAGGGGCCTTCTTCGCCGGGGTCAAGAAGGCCCTGGACCCCAAAGGCCG
CTTTCCCTTAGGGTGAGAGC

Downstream 100 bases:

>100_bases
CGTGCATATCTTCCTGCAGCCATGCTTAAAGCATGGCCCTCACCATTCGCAACAAGGAGGTGGAGCGCCTGGCGGAGGAG
GTGGCCCGCCTCGCCGGAGA

Product: glycolate oxidase iron-sulfur subunit GlcF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MQHQIPVEKLGKEGEVMAHAVEACVHCGFCLPTCPTYVVLQEEMDSPRGRIFLMKEVLEGNLGLEEALPYLDRCLACQAC
VTACPSGVPYGELIATFRLWSEEKRHRFPLERVFRLGLLRTLPYPERFRPLAKLGAGLKPLLRPLPLPKPLKAPLALLPD
RLPEEAPYGEVYPAKGERRARVGLLLGCAQRVLRPSINQATIRVLQENGVEVVAVPEQACCGALNLHAGDKEGARALARK
NLKAFRDVDYVVTNAAGCGSGMKEYPLLFRGEPEEEEARALAAKVKDLSVLLDELGFTPPPPPRRPLRVAYHDACHLAHA
QGVREAPRRLLQAAGLEVLEPREWELCCGSAGTYNLFQPEIAEALGRRKAENLKATGADLVATGNIGCLTQIQAYLDLPV
LHTAELLALLYEGKDPLA

Sequences:

>Translated_418_residues
MQHQIPVEKLGKEGEVMAHAVEACVHCGFCLPTCPTYVVLQEEMDSPRGRIFLMKEVLEGNLGLEEALPYLDRCLACQAC
VTACPSGVPYGELIATFRLWSEEKRHRFPLERVFRLGLLRTLPYPERFRPLAKLGAGLKPLLRPLPLPKPLKAPLALLPD
RLPEEAPYGEVYPAKGERRARVGLLLGCAQRVLRPSINQATIRVLQENGVEVVAVPEQACCGALNLHAGDKEGARALARK
NLKAFRDVDYVVTNAAGCGSGMKEYPLLFRGEPEEEEARALAAKVKDLSVLLDELGFTPPPPPRRPLRVAYHDACHLAHA
QGVREAPRRLLQAAGLEVLEPREWELCCGSAGTYNLFQPEIAEALGRRKAENLKATGADLVATGNIGCLTQIQAYLDLPV
LHTAELLALLYEGKDPLA
>Mature_418_residues
MQHQIPVEKLGKEGEVMAHAVEACVHCGFCLPTCPTYVVLQEEMDSPRGRIFLMKEVLEGNLGLEEALPYLDRCLACQAC
VTACPSGVPYGELIATFRLWSEEKRHRFPLERVFRLGLLRTLPYPERFRPLAKLGAGLKPLLRPLPLPKPLKAPLALLPD
RLPEEAPYGEVYPAKGERRARVGLLLGCAQRVLRPSINQATIRVLQENGVEVVAVPEQACCGALNLHAGDKEGARALARK
NLKAFRDVDYVVTNAAGCGSGMKEYPLLFRGEPEEEEARALAAKVKDLSVLLDELGFTPPPPPRRPLRVAYHDACHLAHA
QGVREAPRRLLQAAGLEVLEPREWELCCGSAGTYNLFQPEIAEALGRRKAENLKATGADLVATGNIGCLTQIQAYLDLPV
LHTAELLALLYEGKDPLA

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=416, Percent_Identity=36.2980769230769, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788576, Length=420, Percent_Identity=28.8095238095238, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786497, Length=236, Percent_Identity=27.5423728813559, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45883; Mature: 45883

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHQIPVEKLGKEGEVMAHAVEACVHCGFCLPTCPTYVVLQEEMDSPRGRIFLMKEVLEG
CCCCCCHHHCCCCCHHHHHHHHHHHHCCCHHCCCCEEEEEHHHHCCCCCEEEEEHHHHCC
NLGLEEALPYLDRCLACQACVTACPSGVPYGELIATFRLWSEEKRHRFPLERVFRLGLLR
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TLPYPERFRPLAKLGAGLKPLLRPLPLPKPLKAPLALLPDRLPEEAPYGEVYPAKGERRA
HCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHCCHHCCCCCCCCCCCCCCCCCHH
RVGLLLGCAQRVLRPSINQATIRVLQENGVEVVAVPEQACCGALNLHAGDKEGARALARK
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCHHHHHHHHHH
NLKAFRDVDYVVTNAAGCGSGMKEYPLLFRGEPEEEEARALAAKVKDLSVLLDELGFTPP
HHHHHHHHHHHHCCCCCCCCCHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PPPRRPLRVAYHDACHLAHAQGVREAPRRLLQAAGLEVLEPREWELCCGSAGTYNLFQPE
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCHH
IAEALGRRKAENLKATGADLVATGNIGCLTQIQAYLDLPVLHTAELLALLYEGKDPLA
HHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQHQIPVEKLGKEGEVMAHAVEACVHCGFCLPTCPTYVVLQEEMDSPRGRIFLMKEVLEG
CCCCCCHHHCCCCCHHHHHHHHHHHHCCCHHCCCCEEEEEHHHHCCCCCEEEEEHHHHCC
NLGLEEALPYLDRCLACQACVTACPSGVPYGELIATFRLWSEEKRHRFPLERVFRLGLLR
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TLPYPERFRPLAKLGAGLKPLLRPLPLPKPLKAPLALLPDRLPEEAPYGEVYPAKGERRA
HCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHCCHHCCCCCCCCCCCCCCCCCHH
RVGLLLGCAQRVLRPSINQATIRVLQENGVEVVAVPEQACCGALNLHAGDKEGARALARK
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCHHHHHHHHHH
NLKAFRDVDYVVTNAAGCGSGMKEYPLLFRGEPEEEEARALAAKVKDLSVLLDELGFTPP
HHHHHHHHHHHHCCCCCCCCCHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PPPRRPLRVAYHDACHLAHAQGVREAPRRLLQAAGLEVLEPREWELCCGSAGTYNLFQPE
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCHH
IAEALGRRKAENLKATGADLVATGNIGCLTQIQAYLDLPVLHTAELLALLYEGKDPLA
HHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]