BacMap

Definition	Thermus thermophilus HB8 chromosome, complete genome.
Accession	NC_006461
Length	1,849,742

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The map label for this gene is yogA [H]

Identifier: 55980435

GI number: 55980435

Start: 435191

End: 436222

Strand: Reverse

Name: yogA [H]

Synonym: TTHA0466

Alternate gene names: 55980435

Gene position: 436222-435191 (Counterclockwise)

Preceding gene: 55980436

Following gene: 55980434

Centisome position: 23.58

GC content: 70.45

Gene sequence:

>1032_bases
ATGCGCGCGGTGGTCATGCGAGCCAGGGGAGGCCCCGAGGTGCTGGAGGTGGCCGACCTTCCCGTCCCCGAACCCGGCCC
CAAGGAGGTGCGGGTACGCCTCAAGGCGGCCGCCCTCAACCACCTGGACGTGTGGGTGCGAAAAGGGGTGGCGAGCCCAA
AGCTCCCCCTCCCCCACGTCCTGGGTGCGGACGGAAGCGGCGTGGTGGACGCCGTGGGGCCGGGGGTGGAGGGCTTCGCC
CCCGGGGACGAAGTGGTCATCAACCCCGGCCTCTCCTGCGGGCGGTGCGAGCGCTGCCTGGCCGGGGAGGACAACCTCTG
CCCCCGTTACCAGATCCTCGGGGAGCACCGGCACGGCACCTACGCCGAGTACGTGGTCCTGCCCGAGGCCAACCTGGCCC
CCAAGCCCAAGAACCTTTCCTTTGAGGAGGCCGCCGCCATCCCCCTCACCTTCCTCACCGCCTGGCAGATGGTGGTGGAC
AAGCTAGGGGTGCGCCCGGGGGACGACGTGCTGGTGATGGCCGCCGGAAGCGGGGTGAGCGTGGCCGCCATCCAGATCGC
CAAGCTCTTTGGGGCGCGGGTCATCGCCACGGCGGGCTCGGAGGACAAACTCCGCCGGGCGAAGGCCCTCGGGGCCGACG
AGACGGTGAACTACACCCACCCGGACTGGCCCAAGGAAGTGCGCCGCCTCACGGGGGGCAAGGGCGCGGACAAGGTGGTG
GACCACACCGGGGCCCTTTACTTTGAGGGGGTGATCAAGGCCACGGCGAACGGGGGACGGATCGCCATCGCCGGGGCCTC
CTCGGGGTACGAGGGCACCCTGCCCTTCGCCCACGTCTTCTACCGTCAGCTCTCCATCCTGGGCTCCACCATGGCCTCTA
AAAGCCGCCTTTTCCCCATCTTGCGCTTCGTGGAGGAGGGGAAGCTGAAGCCCGTGGTGGGCCAGGTCCTGCCCCTGGAG
GCGGCGGCGGAGGGGCACCGGCTCCTGGAGGAGCGCCGGGTCTTCGGGAAGGTGGTCCTCCAGGTGGGGTAG

Upstream 100 bases:

>100_bases
CCCGCCCCCTCCGGGGTACAAGGCGATTTACTAGGCAAGCCCGTCAAGGGTTGACCCCCTAAAGCCCTTCGTGAAAAAAT
CCCCTCCATAAGGAGGGAGC

Downstream 100 bases:

>100_bases
ACAACCCCGCCCACGTGCATAAAATAACAAGCATGGCGGCGGACCACACGGACGTGCTCATCGTAGGGGCGGGGCCTACA
GGGCTTTTCGCCGGGTTTTA

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRAVVMRARGGPEVLEVADLPVPEPGPKEVRVRLKAAALNHLDVWVRKGVASPKLPLPHVLGADGSGVVDAVGPGVEGFA
PGDEVVINPGLSCGRCERCLAGEDNLCPRYQILGEHRHGTYAEYVVLPEANLAPKPKNLSFEEAAAIPLTFLTAWQMVVD
KLGVRPGDDVLVMAAGSGVSVAAIQIAKLFGARVIATAGSEDKLRRAKALGADETVNYTHPDWPKEVRRLTGGKGADKVV
DHTGALYFEGVIKATANGGRIAIAGASSGYEGTLPFAHVFYRQLSILGSTMASKSRLFPILRFVEEGKLKPVVGQVLPLE
AAAEGHRLLEERRVFGKVVLQVG

Sequences:

>Translated_343_residues
MRAVVMRARGGPEVLEVADLPVPEPGPKEVRVRLKAAALNHLDVWVRKGVASPKLPLPHVLGADGSGVVDAVGPGVEGFA
PGDEVVINPGLSCGRCERCLAGEDNLCPRYQILGEHRHGTYAEYVVLPEANLAPKPKNLSFEEAAAIPLTFLTAWQMVVD
KLGVRPGDDVLVMAAGSGVSVAAIQIAKLFGARVIATAGSEDKLRRAKALGADETVNYTHPDWPKEVRRLTGGKGADKVV
DHTGALYFEGVIKATANGGRIAIAGASSGYEGTLPFAHVFYRQLSILGSTMASKSRLFPILRFVEEGKLKPVVGQVLPLE
AAAEGHRLLEERRVFGKVVLQVG
>Mature_343_residues
MRAVVMRARGGPEVLEVADLPVPEPGPKEVRVRLKAAALNHLDVWVRKGVASPKLPLPHVLGADGSGVVDAVGPGVEGFA
PGDEVVINPGLSCGRCERCLAGEDNLCPRYQILGEHRHGTYAEYVVLPEANLAPKPKNLSFEEAAAIPLTFLTAWQMVVD
KLGVRPGDDVLVMAAGSGVSVAAIQIAKLFGARVIATAGSEDKLRRAKALGADETVNYTHPDWPKEVRRLTGGKGADKVV
DHTGALYFEGVIKATANGGRIAIAGASSGYEGTLPFAHVFYRQLSILGSTMASKSRLFPILRFVEEGKLKPVVGQVLPLE
AAAEGHRLLEERRVFGKVVLQVG

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=358, Percent_Identity=31.5642458100559, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI22538444, Length=358, Percent_Identity=31.5642458100559, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI194239674, Length=350, Percent_Identity=28.8571428571429, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI13236495, Length=350, Percent_Identity=28.8571428571429, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194239676, Length=348, Percent_Identity=28.735632183908, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI18379349, Length=336, Percent_Identity=24.702380952381, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI156627571, Length=330, Percent_Identity=30, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI47519420, Length=383, Percent_Identity=28.1984334203655, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI24308257, Length=375, Percent_Identity=25.8666666666667, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI71565152, Length=367, Percent_Identity=25.6130790190736, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=25.8152173913043, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI34577061, Length=238, Percent_Identity=29.8319327731092, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4501939, Length=358, Percent_Identity=26.2569832402235, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI71565154, Length=238, Percent_Identity=29.8319327731092, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI262073058, Length=364, Percent_Identity=25, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI71743840, Length=364, Percent_Identity=25, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI4501933, Length=238, Percent_Identity=28.9915966386555, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4501929, Length=241, Percent_Identity=29.045643153527, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI197927207, Length=186, Percent_Identity=28.494623655914, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI28557745, Length=281, Percent_Identity=27.7580071174377, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI67078404, Length=223, Percent_Identity=30.0448430493274, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI41872631, Length=234, Percent_Identity=26.0683760683761, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI226059159, Length=171, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI226059133, Length=171, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790485, Length=342, Percent_Identity=28.0701754385965, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790045, Length=337, Percent_Identity=27.893175074184, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788407, Length=356, Percent_Identity=27.8089887640449, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787863, Length=342, Percent_Identity=26.3157894736842, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788073, Length=266, Percent_Identity=29.3233082706767, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI87081918, Length=315, Percent_Identity=27.6190476190476, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082125, Length=345, Percent_Identity=28.4057971014493, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI226510992, Length=291, Percent_Identity=25.7731958762887, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=28.1842818428184, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788075, Length=354, Percent_Identity=25.1412429378531, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786518, Length=242, Percent_Identity=31.8181818181818, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507255, Length=350, Percent_Identity=27.4285714285714, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17562876, Length=264, Percent_Identity=32.1969696969697, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71988145, Length=343, Percent_Identity=27.6967930029155, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17562584, Length=347, Percent_Identity=27.3775216138329, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17562582, Length=323, Percent_Identity=28.7925696594427, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17562878, Length=207, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25146526, Length=225, Percent_Identity=31.1111111111111, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71997431, Length=227, Percent_Identity=30.8370044052863, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565904, Length=235, Percent_Identity=28.936170212766, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17536829, Length=304, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319520, Length=353, Percent_Identity=26.628895184136, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6319621, Length=349, Percent_Identity=26.9340974212034, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323961, Length=346, Percent_Identity=26.0115606936416, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319955, Length=339, Percent_Identity=29.4985250737463, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322619, Length=339, Percent_Identity=29.4985250737463, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324486, Length=335, Percent_Identity=25.9701492537313, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=335, Percent_Identity=24.7761194029851, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320033, Length=252, Percent_Identity=26.5873015873016, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323099, Length=329, Percent_Identity=26.4437689969605, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319258, Length=232, Percent_Identity=29.7413793103448, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17737895, Length=234, Percent_Identity=25.6410256410256, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137530, Length=296, Percent_Identity=25.6756756756757, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550770, Length=186, Percent_Identity=30.6451612903226, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45551930, Length=186, Percent_Identity=30.6451612903226, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221457811, Length=186, Percent_Identity=30.6451612903226, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17737897, Length=295, Percent_Identity=26.1016949152542, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 36421; Mature: 36421

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVVMRARGGPEVLEVADLPVPEPGPKEVRVRLKAAALNHLDVWVRKGVASPKLPLPHV
CCEEEEECCCCCCEEEEECCCCCCCCCHHHEEEEHHHHHHHHHHHHHHCCCCCCCCCCCE
LGADGSGVVDAVGPGVEGFAPGDEVVINPGLSCGRCERCLAGEDNLCPRYQILGEHRHGT
ECCCCCCEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCC
YAEYVVLPEANLAPKPKNLSFEEAAAIPLTFLTAWQMVVDKLGVRPGDDVLVMAAGSGVS
EEEEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
VAAIQIAKLFGARVIATAGSEDKLRRAKALGADETVNYTHPDWPKEVRRLTGGKGADKVV
HHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHH
DHTGALYFEGVIKATANGGRIAIAGASSGYEGTLPFAHVFYRQLSILGSTMASKSRLFPI
HHCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRFVEEGKLKPVVGQVLPLEAAAEGHRLLEERRVFGKVVLQVG
HHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MRAVVMRARGGPEVLEVADLPVPEPGPKEVRVRLKAAALNHLDVWVRKGVASPKLPLPHV
CCEEEEECCCCCCEEEEECCCCCCCCCHHHEEEEHHHHHHHHHHHHHHCCCCCCCCCCCE
LGADGSGVVDAVGPGVEGFAPGDEVVINPGLSCGRCERCLAGEDNLCPRYQILGEHRHGT
ECCCCCCEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCC
YAEYVVLPEANLAPKPKNLSFEEAAAIPLTFLTAWQMVVDKLGVRPGDDVLVMAAGSGVS
EEEEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
VAAIQIAKLFGARVIATAGSEDKLRRAKALGADETVNYTHPDWPKEVRRLTGGKGADKVV
HHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHH
DHTGALYFEGVIKATANGGRIAIAGASSGYEGTLPFAHVFYRQLSILGSTMASKSRLFPI
HHCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRFVEEGKLKPVVGQVLPLEAAAEGHRLLEERRVFGKVVLQVG
HHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]