| Definition | Thermus thermophilus HB8 chromosome, complete genome. |
|---|---|
| Accession | NC_006461 |
| Length | 1,849,742 |
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The map label for this gene is hdhA [C]
Identifier: 55980338
GI number: 55980338
Start: 352186
End: 352956
Strand: Direct
Name: hdhA [C]
Synonym: TTHA0369
Alternate gene names: 55980338
Gene position: 352186-352956 (Clockwise)
Preceding gene: 55980337
Following gene: 55980340
Centisome position: 19.04
GC content: 72.11
Gene sequence:
>771_bases ATGGGCCTTTTCGCTGGCAAAGGGGTGCTGGTGACCGGGGGGGCCCGGGGCATCGGCCGGGCCATCGCCCAGGCCTTCGC CCGGGAGGGGGCCTTGGTGGCCCTGTGCGACCTCCGCCCGGAGGGGAAGGAGGTGGCGGAGGCCATCGGGGGGGCGTTTT TCCAGGTGGACCTCGAGGACGAGAGGGAGCGGGTGCGCTTCGTGGAGGAGGCCGCCTACGCCCTGGGCCGGGTGGACGTT CTGGTCAACAACGCCGCCATCGCCGCCCCCGGCTCGGCCCTCACGGTGCGGCTTCCCGAGTGGCGCAGGGTGCTTGAGGT CAACCTCACCGCCCCCATGCACCTTTCCGCCTTGGCCGCGCGGGAGATGCGGAAGGTGGGCGGTGGGGCCATCGTCAACG TGGCCAGCGTGCAGGGGCTTTTCGCCGAGCAGGAAAACGCCGCCTACAACGCTTCCAAGGGGGGGCTTGTGAACCTCACC CGCTCCCTGGCGCTGGACCTCGCCCCCCTCCGCATCCGGGTGAACGCGGTGGCGCCCGGGGCCATCGCCACGGAGGCGGT CCTGGAGGCCATCGCCCTCTCCCCGGACCCGGAGAGGACCCGGAGGGACTGGGAGGACCTCCACGCCCTGAGGCGCCTGG GGAAGCCCGAGGAGGTGGCGGAGGCCGTCCTCTTCCTGGCCTCGGAGAAGGCCAGCTTCATCACCGGGGCCATCCTGCCC GTGGACGGCGGGATGACGGCGAGCTTCATGATGGCGGGGCGGCCGGTGTAG
Upstream 100 bases:
>100_bases CCCCCCTCAGGGCAGCGCAAAAGGGAAGATACCAAGGAGGCATGGCCTTTGTCAAGGGCCCCCTCTTGACGGGAGGCCCT CGGGGGAAAATCCTTCGGGT
Downstream 100 bases:
>100_bases GCCCCCACCGGGGCGAGGGCCCCGCGGAGGCGAAGCGCGTGGGGACTAGAGGGCGTGGTCCTTGTAGGGCTCGCCCCGCA GGTCGGAGACCAGGTGGACG
Product: short chain dehydrogenase/reductase family oxidoreductase
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: NA
Number of amino acids: Translated: 256; Mature: 255
Protein sequence:
>256_residues MGLFAGKGVLVTGGARGIGRAIAQAFAREGALVALCDLRPEGKEVAEAIGGAFFQVDLEDERERVRFVEEAAYALGRVDV LVNNAAIAAPGSALTVRLPEWRRVLEVNLTAPMHLSALAAREMRKVGGGAIVNVASVQGLFAEQENAAYNASKGGLVNLT RSLALDLAPLRIRVNAVAPGAIATEAVLEAIALSPDPERTRRDWEDLHALRRLGKPEEVAEAVLFLASEKASFITGAILP VDGGMTASFMMAGRPV
Sequences:
>Translated_256_residues MGLFAGKGVLVTGGARGIGRAIAQAFAREGALVALCDLRPEGKEVAEAIGGAFFQVDLEDERERVRFVEEAAYALGRVDV LVNNAAIAAPGSALTVRLPEWRRVLEVNLTAPMHLSALAAREMRKVGGGAIVNVASVQGLFAEQENAAYNASKGGLVNLT RSLALDLAPLRIRVNAVAPGAIATEAVLEAIALSPDPERTRRDWEDLHALRRLGKPEEVAEAVLFLASEKASFITGAILP VDGGMTASFMMAGRPV >Mature_255_residues GLFAGKGVLVTGGARGIGRAIAQAFAREGALVALCDLRPEGKEVAEAIGGAFFQVDLEDERERVRFVEEAAYALGRVDVL VNNAAIAAPGSALTVRLPEWRRVLEVNLTAPMHLSALAAREMRKVGGGAIVNVASVQGLFAEQENAAYNASKGGLVNLTR SLALDLAPLRIRVNAVAPGAIATEAVLEAIALSPDPERTRRDWEDLHALRRLGKPEEVAEAVLFLASEKASFITGAILPV DGGMTASFMMAGRPV
Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]
COG id: COG1028
COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=37.6, Blast_Score=131, Evalue=6e-31, Organism=Homo sapiens, GI40254992, Length=238, Percent_Identity=38.655462184874, Blast_Score=130, Evalue=1e-30, Organism=Homo sapiens, GI59889578, Length=229, Percent_Identity=36.6812227074236, Blast_Score=121, Evalue=8e-28, Organism=Homo sapiens, GI7705925, Length=252, Percent_Identity=38.0952380952381, Blast_Score=116, Evalue=2e-26, Organism=Homo sapiens, GI15277342, Length=251, Percent_Identity=33.4661354581673, Blast_Score=115, Evalue=3e-26, Organism=Homo sapiens, GI19923817, Length=255, Percent_Identity=32.5490196078431, Blast_Score=110, Evalue=1e-24, Organism=Homo sapiens, GI5031737, Length=256, Percent_Identity=33.59375, Blast_Score=107, Evalue=9e-24, Organism=Homo sapiens, GI4758504, Length=252, Percent_Identity=34.5238095238095, Blast_Score=101, Evalue=7e-22, Organism=Homo sapiens, GI31542939, Length=252, Percent_Identity=31.3492063492063, Blast_Score=100, Evalue=1e-21, Organism=Homo sapiens, GI10190704, Length=256, Percent_Identity=29.296875, Blast_Score=100, Evalue=2e-21, Organism=Homo sapiens, GI66933014, Length=248, Percent_Identity=30.241935483871, Blast_Score=97, Evalue=1e-20, Organism=Homo sapiens, GI4504505, Length=245, Percent_Identity=34.6938775510204, Blast_Score=95, Evalue=6e-20, Organism=Homo sapiens, GI126723750, Length=253, Percent_Identity=32.8063241106719, Blast_Score=92, Evalue=4e-19, Organism=Homo sapiens, GI33667109, Length=195, Percent_Identity=35.3846153846154, Blast_Score=86, Evalue=2e-17, Organism=Homo sapiens, GI109715829, Length=243, Percent_Identity=28.8065843621399, Blast_Score=85, Evalue=5e-17, Organism=Homo sapiens, GI83715985, Length=252, Percent_Identity=32.5396825396825, Blast_Score=84, Evalue=9e-17, Organism=Homo sapiens, GI126723191, Length=184, Percent_Identity=33.695652173913, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI224922801, Length=170, Percent_Identity=34.7058823529412, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI20149619, Length=246, Percent_Identity=27.6422764227642, Blast_Score=74, Evalue=1e-13, Organism=Homo sapiens, GI142976729, Length=246, Percent_Identity=26.8292682926829, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI302344755, Length=103, Percent_Identity=37.8640776699029, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI210032110, Length=189, Percent_Identity=28.042328042328, Blast_Score=69, Evalue=4e-12, Organism=Homo sapiens, GI223718074, Length=267, Percent_Identity=29.2134831460674, Blast_Score=68, Evalue=8e-12, Organism=Homo sapiens, GI4503817, Length=230, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI32455239, Length=199, Percent_Identity=29.1457286432161, Blast_Score=65, Evalue=8e-11, Organism=Homo sapiens, GI5031765, Length=199, Percent_Identity=29.1457286432161, Blast_Score=65, Evalue=8e-11, Organism=Escherichia coli, GI1787905, Length=248, Percent_Identity=37.9032258064516, Blast_Score=136, Evalue=1e-33, Organism=Escherichia coli, GI1787335, Length=245, Percent_Identity=37.5510204081633, Blast_Score=128, Evalue=3e-31, Organism=Escherichia coli, GI87082100, Length=255, Percent_Identity=34.9019607843137, Blast_Score=128, Evalue=4e-31, Organism=Escherichia coli, GI2367365, Length=250, Percent_Identity=37.6, Blast_Score=127, Evalue=6e-31, Organism=Escherichia coli, GI87082160, Length=251, Percent_Identity=33.0677290836653, Blast_Score=126, Evalue=1e-30, Organism=Escherichia coli, GI1788459, Length=252, Percent_Identity=35.3174603174603, Blast_Score=121, Evalue=5e-29, Organism=Escherichia coli, GI1790717, Length=248, Percent_Identity=33.8709677419355, Blast_Score=116, Evalue=2e-27, Organism=Escherichia coli, GI1786812, Length=250, Percent_Identity=33.2, Blast_Score=105, Evalue=4e-24, Organism=Escherichia coli, GI2367175, Length=247, Percent_Identity=38.4615384615385, Blast_Score=104, Evalue=5e-24, Organism=Escherichia coli, GI1789208, Length=247, Percent_Identity=31.1740890688259, Blast_Score=100, Evalue=8e-23, Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=2e-21, Organism=Escherichia coli, GI1789057, Length=250, Percent_Identity=29.6, Blast_Score=96, Evalue=3e-21, Organism=Escherichia coli, GI1787820, Length=229, Percent_Identity=26.2008733624454, Blast_Score=64, Evalue=8e-12, Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=36.6533864541833, Blast_Score=133, Evalue=9e-32, Organism=Caenorhabditis elegans, GI17560676, Length=245, Percent_Identity=33.8775510204082, Blast_Score=131, Evalue=3e-31, Organism=Caenorhabditis elegans, GI17561402, Length=257, Percent_Identity=35.408560311284, Blast_Score=123, Evalue=1e-28, Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=33.4661354581673, Blast_Score=121, Evalue=3e-28, Organism=Caenorhabditis elegans, GI72000259, Length=269, Percent_Identity=34.9442379182156, Blast_Score=111, Evalue=4e-25, Organism=Caenorhabditis elegans, GI17562910, Length=267, Percent_Identity=32.9588014981273, Blast_Score=107, Evalue=4e-24, Organism=Caenorhabditis elegans, GI17563726, Length=266, Percent_Identity=31.203007518797, Blast_Score=105, Evalue=2e-23, Organism=Caenorhabditis elegans, GI71994600, Length=254, Percent_Identity=32.6771653543307, Blast_Score=105, Evalue=2e-23, Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=33.201581027668, Blast_Score=105, Evalue=3e-23, Organism=Caenorhabditis elegans, GI17562904, Length=268, Percent_Identity=32.089552238806, Blast_Score=103, Evalue=8e-23, Organism=Caenorhabditis elegans, GI17560150, Length=268, Percent_Identity=32.8358208955224, Blast_Score=103, Evalue=9e-23, Organism=Caenorhabditis elegans, GI17562990, Length=271, Percent_Identity=31.3653136531365, Blast_Score=101, Evalue=3e-22, Organism=Caenorhabditis elegans, GI17560332, Length=267, Percent_Identity=29.2134831460674, Blast_Score=100, Evalue=6e-22, Organism=Caenorhabditis elegans, GI17559104, Length=273, Percent_Identity=31.1355311355311, Blast_Score=100, Evalue=6e-22, Organism=Caenorhabditis elegans, GI17538480, Length=266, Percent_Identity=31.203007518797, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17538182, Length=252, Percent_Identity=32.9365079365079, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI115534694, Length=260, Percent_Identity=31.1538461538462, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17562906, Length=265, Percent_Identity=31.6981132075472, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17531453, Length=266, Percent_Identity=32.3308270676692, Blast_Score=95, Evalue=3e-20, Organism=Caenorhabditis elegans, GI17536651, Length=255, Percent_Identity=30.1960784313725, Blast_Score=94, Evalue=8e-20, Organism=Caenorhabditis elegans, GI193204405, Length=270, Percent_Identity=29.6296296296296, Blast_Score=94, Evalue=8e-20, Organism=Caenorhabditis elegans, GI17562908, Length=265, Percent_Identity=30.9433962264151, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17536025, Length=256, Percent_Identity=31.25, Blast_Score=90, Evalue=9e-19, Organism=Caenorhabditis elegans, GI17565030, Length=274, Percent_Identity=29.9270072992701, Blast_Score=84, Evalue=8e-17, Organism=Caenorhabditis elegans, GI17551412, Length=265, Percent_Identity=29.0566037735849, Blast_Score=82, Evalue=3e-16, Organism=Caenorhabditis elegans, GI17560220, Length=265, Percent_Identity=28.6792452830189, Blast_Score=81, Evalue=5e-16, Organism=Caenorhabditis elegans, GI17561272, Length=241, Percent_Identity=30.7053941908714, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17508651, Length=251, Percent_Identity=29.8804780876494, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17544670, Length=263, Percent_Identity=26.9961977186312, Blast_Score=77, Evalue=6e-15, Organism=Caenorhabditis elegans, GI193203117, Length=246, Percent_Identity=28.8617886178862, Blast_Score=73, Evalue=2e-13, Organism=Caenorhabditis elegans, GI17538486, Length=267, Percent_Identity=27.3408239700375, Blast_Score=72, Evalue=2e-13, Organism=Caenorhabditis elegans, GI193203115, Length=245, Percent_Identity=28.9795918367347, Blast_Score=72, Evalue=3e-13, Organism=Caenorhabditis elegans, GI17564282, Length=188, Percent_Identity=31.9148936170213, Blast_Score=71, Evalue=6e-13, Organism=Caenorhabditis elegans, GI17567345, Length=168, Percent_Identity=29.7619047619048, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI71997099, Length=202, Percent_Identity=32.6732673267327, Blast_Score=69, Evalue=2e-12, Organism=Caenorhabditis elegans, GI17508895, Length=187, Percent_Identity=28.8770053475936, Blast_Score=68, Evalue=5e-12, Organism=Caenorhabditis elegans, GI17568967, Length=212, Percent_Identity=28.7735849056604, Blast_Score=66, Evalue=1e-11, Organism=Saccharomyces cerevisiae, GI6322861, Length=255, Percent_Identity=32.5490196078431, Blast_Score=95, Evalue=1e-20, Organism=Saccharomyces cerevisiae, GI6324126, Length=253, Percent_Identity=27.6679841897233, Blast_Score=84, Evalue=2e-17, Organism=Saccharomyces cerevisiae, GI6322226, Length=186, Percent_Identity=27.4193548387097, Blast_Score=68, Evalue=2e-12, Organism=Saccharomyces cerevisiae, GI6323882, Length=248, Percent_Identity=26.2096774193548, Blast_Score=65, Evalue=2e-11, Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=42.6294820717131, Blast_Score=146, Evalue=2e-35, Organism=Drosophila melanogaster, GI28571526, Length=254, Percent_Identity=40.5511811023622, Blast_Score=137, Evalue=9e-33, Organism=Drosophila melanogaster, GI21357041, Length=257, Percent_Identity=37.7431906614786, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI23397609, Length=248, Percent_Identity=36.2903225806452, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI24644337, Length=253, Percent_Identity=40.3162055335968, Blast_Score=122, Evalue=2e-28, Organism=Drosophila melanogaster, GI21355319, Length=263, Percent_Identity=34.2205323193916, Blast_Score=109, Evalue=1e-24, Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=32.5396825396825, Blast_Score=107, Evalue=6e-24, Organism=Drosophila melanogaster, GI17737361, Length=252, Percent_Identity=34.1269841269841, Blast_Score=100, Evalue=7e-22, Organism=Drosophila melanogaster, GI24643142, Length=247, Percent_Identity=31.5789473684211, Blast_Score=93, Evalue=2e-19, Organism=Drosophila melanogaster, GI21358495, Length=197, Percent_Identity=30.9644670050761, Blast_Score=80, Evalue=9e-16, Organism=Drosophila melanogaster, GI18859837, Length=207, Percent_Identity=29.4685990338164, Blast_Score=74, Evalue=8e-14, Organism=Drosophila melanogaster, GI221331218, Length=253, Percent_Identity=28.4584980237154, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI24665243, Length=220, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=2e-12, Organism=Drosophila melanogaster, GI24580925, Length=201, Percent_Identity=30.3482587064677, Blast_Score=68, Evalue=5e-12, Organism=Drosophila melanogaster, GI24649181, Length=193, Percent_Identity=26.4248704663212, Blast_Score=68, Evalue=6e-12, Organism=Drosophila melanogaster, GI221331216, Length=254, Percent_Identity=28.740157480315, Blast_Score=65, Evalue=4e-11, Organism=Drosophila melanogaster, GI24665247, Length=221, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=4e-11, Organism=Drosophila melanogaster, GI24584752, Length=183, Percent_Identity=30.6010928961749, Blast_Score=65, Evalue=6e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 26961; Mature: 26830
Theoretical pI: Translated: 5.46; Mature: 5.46
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGLFAGKGVLVTGGARGIGRAIAQAFAREGALVALCDLRPEGKEVAEAIGGAFFQVDLED CCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCEEEEEECCC ERERVRFVEEAAYALGRVDVLVNNAAIAAPGSALTVRLPEWRRVLEVNLTAPMHLSALAA HHHHHHHHHHHHHHHHHEEEEEECCEEECCCCEEEEECHHHHEEEEEECCCCHHHHHHHH REMRKVGGGAIVNVASVQGLFAEQENAAYNASKGGLVNLTRSLALDLAPLRIRVNAVAPG HHHHHHCCCEEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEEEEEECCC AIATEAVLEAIALSPDPERTRRDWEDLHALRRLGKPEEVAEAVLFLASEKASFITGAILP HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHEEEEEE VDGGMTASFMMAGRPV CCCCCEEEEEECCCCC >Mature Secondary Structure GLFAGKGVLVTGGARGIGRAIAQAFAREGALVALCDLRPEGKEVAEAIGGAFFQVDLED CCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCEEEEEECCC ERERVRFVEEAAYALGRVDVLVNNAAIAAPGSALTVRLPEWRRVLEVNLTAPMHLSALAA HHHHHHHHHHHHHHHHHEEEEEECCEEECCCCEEEEECHHHHEEEEEECCCCHHHHHHHH REMRKVGGGAIVNVASVQGLFAEQENAAYNASKGGLVNLTRSLALDLAPLRIRVNAVAPG HHHHHHCCCEEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEEEEEECCC AIATEAVLEAIALSPDPERTRRDWEDLHALRRLGKPEEVAEAVLFLASEKASFITGAILP HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHEEEEEE VDGGMTASFMMAGRPV CCCCCEEEEEECCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 10360571 [H]