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The map label for this gene is rutB [H]

Identifier: 55980297

GI number: 55980297

Start: 315142

End: 315732

Strand: Direct

Name: rutB [H]

Synonym: TTHA0328

Alternate gene names: 55980297

Gene position: 315142-315732 (Clockwise)

Preceding gene: 55980285

Following gene: 55980298

Centisome position: 17.04

GC content: 67.34

Gene sequence:

>591_bases
ATGGTCCAGGTGCCCGAGATCCCCAAGGTGGAAACCGTGGAACTCCCCGCCAAGGAGACGGCCCTCATCGTGGTGGACAT
GCAGAACGACTTCGCCCACCCCAAGGGCGCCCTCTTCGTCCCCGACGCCCCCCAAAGCGTCCCCGCCATCCGGCTCCTCC
TGGAGAGGGCGCGGCAAGCGGGGGCCAAGGTGGTCTACACCCAGGACTGGCACCGGGAGGACGACCCCGAGTTCCAGATC
TGGCCCCGGCACGCCGTGGCCGGGACCTGGGGCGCGGAGATCCTGGAGGAGCTTAAGCCCGAGCCCGAAGACCTCGTCAT
CCGGAAGGTCCGCTACGACGCCTTCTACGGCACCCCCTTGGACCACTACCTGCACCTCTTCGGGGTAAAGCACGTGGTGG
TCACGGGGACGGTGGCCAACATCTGCGTCCTGCACACGGCGGGGTCCGCGGCCTTGCGCTGGTACAACGTGGTCCTTCCC
GAGGATGCCACCAGCGCCCTCACCCCCTTTGACCTGGAGGCCACCTTGCGCCAGGTGACCTTCCTCTACCAGGGCAAGGT
CACCCGGGCCGAGGGGGTTCGGTTTGTTTGA

Upstream 100 bases:

>100_bases
AGCGGGCCCCGCCGCGCCTTGAGGAGGTCCCGTATTTTCATGCGAACGAGTGTAGCCCCCCTCCTCCCCGGGGTCAAGGC
GGCCGGGGTACCATGAGGGC

Downstream 100 bases:

>100_bases
GCACCCCGTCCTCGCCGCCTTTTACCGGCGGCACGGCCCCGCCCCCTTCGCCCCCAACCCCTTTCCCCAAAGGCCCCCCT
TCCGCGTGCTCGCGGAGAGC

Product: isochorismatase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MVQVPEIPKVETVELPAKETALIVVDMQNDFAHPKGALFVPDAPQSVPAIRLLLERARQAGAKVVYTQDWHREDDPEFQI
WPRHAVAGTWGAEILEELKPEPEDLVIRKVRYDAFYGTPLDHYLHLFGVKHVVVTGTVANICVLHTAGSAALRWYNVVLP
EDATSALTPFDLEATLRQVTFLYQGKVTRAEGVRFV

Sequences:

>Translated_196_residues
MVQVPEIPKVETVELPAKETALIVVDMQNDFAHPKGALFVPDAPQSVPAIRLLLERARQAGAKVVYTQDWHREDDPEFQI
WPRHAVAGTWGAEILEELKPEPEDLVIRKVRYDAFYGTPLDHYLHLFGVKHVVVTGTVANICVLHTAGSAALRWYNVVLP
EDATSALTPFDLEATLRQVTFLYQGKVTRAEGVRFV
>Mature_196_residues
MVQVPEIPKVETVELPAKETALIVVDMQNDFAHPKGALFVPDAPQSVPAIRLLLERARQAGAKVVYTQDWHREDDPEFQI
WPRHAVAGTWGAEILEELKPEPEDLVIRKVRYDAFYGTPLDHYLHLFGVKHVVVTGTVANICVLHTAGSAALRWYNVVLP
EDATSALTPFDLEATLRQVTFLYQGKVTRAEGVRFV

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=185, Percent_Identity=31.8918918918919, Blast_Score=89, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321401, Length=191, Percent_Identity=26.1780104712042, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 21977; Mature: 21977

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQVPEIPKVETVELPAKETALIVVDMQNDFAHPKGALFVPDAPQSVPAIRLLLERARQA
CCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHC
GAKVVYTQDWHREDDPEFQIWPRHAVAGTWGAEILEELKPEPEDLVIRKVRYDAFYGTPL
CCEEEEECCCCCCCCCCEEEECCHHCCCCHHHHHHHHHCCCCHHHEEEEEEECEEECCCH
DHYLHLFGVKHVVVTGTVANICVLHTAGSAALRWYNVVLPEDATSALTPFDLEATLRQVT
HHHHHHHHHCEEEEECCHHHEEEEECCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHH
FLYQGKVTRAEGVRFV
HHHCCCEECCCCCCCC
>Mature Secondary Structure
MVQVPEIPKVETVELPAKETALIVVDMQNDFAHPKGALFVPDAPQSVPAIRLLLERARQA
CCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHC
GAKVVYTQDWHREDDPEFQIWPRHAVAGTWGAEILEELKPEPEDLVIRKVRYDAFYGTPL
CCEEEEECCCCCCCCCCEEEECCHHCCCCHHHHHHHHHCCCCHHHEEEEEEECEEECCCH
DHYLHLFGVKHVVVTGTVANICVLHTAGSAALRWYNVVLPEDATSALTPFDLEATLRQVT
HHHHHHHHHCEEEEECCHHHEEEEECCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHH
FLYQGKVTRAEGVRFV
HHHCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA