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The map label for this gene is benM [H]

Identifier: 55980155

GI number: 55980155

Start: 183132

End: 183989

Strand: Direct

Name: benM [H]

Synonym: TTHA0186

Alternate gene names: 55980155

Gene position: 183132-183989 (Clockwise)

Preceding gene: 55980152

Following gene: 55980156

Centisome position: 9.9

GC content: 73.43

Gene sequence:

>858_bases
ATGGACCCGCGCCGCCTCAAGGCCTTCCTGGTCCTGGCGGAGGAAAAGAGCTTCCACAAGGCTGCGGAGCGCCTCTACCT
CTCCCAGCCCGCCCTCACCCAGAGGATCCAGGCCCTGGAGCGGGAGCTCGGGGTGCGCCTTCTGGAAAGGCGCCCCTTCC
GCCTGACCCCGGCGGGGGATCTGCTCCGCCGGGAAGGGGCGCGGCTCCTTAAGGAGCTGGAGGCCCTCAAGGAGGCGGTC
CGGCGGGCGGGGCAGGAGGCCCTCCGCTTCGGGGTGCCGGAGAACCTCCTCCCCGACCTCATGCCCCTCCTGGACCACCT
GCGCCGGGGGCTCGGCCAGGCGGTGGAGGTTCTGGAGATGCACACCCCCGAGCAGGTCCGGGCCCTCAAGGAGGGGCGCC
TGGACTACGGCCTCGCGGGGCTCAAGGTGGAGGACCCGGAGGTGGCGAAGGAGCCCCTCCTCCGGGTGCCCATCGTGGTG
GCCCTGCCCGAAACCCACCCCCTGGCCCCTAGGGCCCGGGTGCCCCTTCGGGCCCTGAAGGAGGAGCCCTTCCTCCTCCT
TCCCAAGGACTTCCTCCCCCCCCTCTACGAGGCCTTCATGGAGGTCTTCCGCCGGGCGGGCTTCGCCCCCAAGGTGGTCC
GGGAGGTGGCCCGCTTCTCCCAGGCGGTGAGCCTGGTGGCCGCGGGCCTCGGGGTCCACCTCACCCTCGCCCCCTACCGG
GTCTACCCCCACCCCGGGGTGGTCCTGAGGCCCCTGGAGGAGGAGGCCGCCCTCGAGGTGGCCCTCATCTACGCCCGGCG
CCCGCCCCCGCCCAGGCTGGAGGAGGTGCGCCGGCTCCTCCGCGCCCTCGCCCTGTAG

Upstream 100 bases:

>100_bases
CTCCAAAACTACACTACACCTTCCCAGGACCAGTCTGCCCTCGAGGAGGTCCCCGCGTCCAATAAGACCTTCGCCGCGGT
TGTGATAAGATCTTTTTATC

Downstream 100 bases:

>100_bases
AATGCCCCCATGGACCGCCTCGAGGCCGCCCTGAAGACCCACCCCGGCCTCAAGCGCCCCAAGAACGAGGACGCGGTGGG
CGGGGAGGCCACCCCCTGGG

Product: transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MDPRRLKAFLVLAEEKSFHKAAERLYLSQPALTQRIQALERELGVRLLERRPFRLTPAGDLLRREGARLLKELEALKEAV
RRAGQEALRFGVPENLLPDLMPLLDHLRRGLGQAVEVLEMHTPEQVRALKEGRLDYGLAGLKVEDPEVAKEPLLRVPIVV
ALPETHPLAPRARVPLRALKEEPFLLLPKDFLPPLYEAFMEVFRRAGFAPKVVREVARFSQAVSLVAAGLGVHLTLAPYR
VYPHPGVVLRPLEEEAALEVALIYARRPPPPRLEEVRRLLRALAL

Sequences:

>Translated_285_residues
MDPRRLKAFLVLAEEKSFHKAAERLYLSQPALTQRIQALERELGVRLLERRPFRLTPAGDLLRREGARLLKELEALKEAV
RRAGQEALRFGVPENLLPDLMPLLDHLRRGLGQAVEVLEMHTPEQVRALKEGRLDYGLAGLKVEDPEVAKEPLLRVPIVV
ALPETHPLAPRARVPLRALKEEPFLLLPKDFLPPLYEAFMEVFRRAGFAPKVVREVARFSQAVSLVAAGLGVHLTLAPYR
VYPHPGVVLRPLEEEAALEVALIYARRPPPPRLEEVRRLLRALAL
>Mature_285_residues
MDPRRLKAFLVLAEEKSFHKAAERLYLSQPALTQRIQALERELGVRLLERRPFRLTPAGDLLRREGARLLKELEALKEAV
RRAGQEALRFGVPENLLPDLMPLLDHLRRGLGQAVEVLEMHTPEQVRALKEGRLDYGLAGLKVEDPEVAKEPLLRVPIVV
ALPETHPLAPRARVPLRALKEEPFLLLPKDFLPPLYEAFMEVFRRAGFAPKVVREVARFSQAVSLVAAGLGVHLTLAPYR
VYPHPGVVLRPLEEEAALEVALIYARRPPPPRLEEVRRLLRALAL

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=270, Percent_Identity=31.4814814814815, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI2367136, Length=280, Percent_Identity=31.0714285714286, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI157672245, Length=233, Percent_Identity=33.9055793991416, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1788887, Length=265, Percent_Identity=31.6981132075472, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790399, Length=298, Percent_Identity=32.5503355704698, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788748, Length=248, Percent_Identity=29.4354838709677, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693105, Length=184, Percent_Identity=31.5217391304348, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788297, Length=261, Percent_Identity=29.5019157088123, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=238, Percent_Identity=27.7310924369748, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790262, Length=241, Percent_Identity=25.3112033195021, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32230; Mature: 32230

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPRRLKAFLVLAEEKSFHKAAERLYLSQPALTQRIQALERELGVRLLERRPFRLTPAGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LLRREGARLLKELEALKEAVRRAGQEALRFGVPENLLPDLMPLLDHLRRGLGQAVEVLEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTPEQVRALKEGRLDYGLAGLKVEDPEVAKEPLLRVPIVVALPETHPLAPRARVPLRALK
CCHHHHHHHHHCCCCCCCCCCEECCHHHHHCCHHHCCEEEEECCCCCCCCCCCCCHHHHC
EEPFLLLPKDFLPPLYEAFMEVFRRAGFAPKVVREVARFSQAVSLVAAGLGVHLTLAPYR
CCCCEECCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECEE
VYPHPGVVLRPLEEEAALEVALIYARRPPPPRLEEVRRLLRALAL
ECCCCCCEEECCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDPRRLKAFLVLAEEKSFHKAAERLYLSQPALTQRIQALERELGVRLLERRPFRLTPAGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LLRREGARLLKELEALKEAVRRAGQEALRFGVPENLLPDLMPLLDHLRRGLGQAVEVLEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTPEQVRALKEGRLDYGLAGLKVEDPEVAKEPLLRVPIVVALPETHPLAPRARVPLRALK
CCHHHHHHHHHCCCCCCCCCCEECCHHHHHCCHHHCCEEEEECCCCCCCCCCCCCHHHHC
EEPFLLLPKDFLPPLYEAFMEVFRRAGFAPKVVREVARFSQAVSLVAAGLGVHLTLAPYR
CCCCEECCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECEE
VYPHPGVVLRPLEEEAALEVALIYARRPPPPRLEEVRRLLRALAL
ECCCCCCEEECCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]