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The map label for this gene is aceF [H]

Identifier: 55980153

GI number: 55980153

Start: 179036

End: 180298

Strand: Reverse

Name: aceF [H]

Synonym: TTHA0184

Alternate gene names: 55980153

Gene position: 180298-179036 (Counterclockwise)

Preceding gene: 55980154

Following gene: 55980149

Centisome position: 9.75

GC content: 71.18

Gene sequence:

>1263_bases
ATGGAGGTCAAGCTACCCGAACTCGGCGACAACGTAACGCAGGCGACCGTGGTGGGCGTCCTGGTGAAGGAAGGGGACCG
GGTGGAGCCGGGCCAGCCCCTTCTGGAGCTGGAGACGGACAAGGCGGTGGTGGAGGTGCCCGCGGAGGCGGGCGGCGTGG
TGAAGCGGGTTTTGGTCAAGGTGGGGGACGAGGTGCGCCCGGGACAGCCCTTCCTGGAGCTCGCCGAGGCCGAGGGCGGG
GCGGAAGCGCCTCCCCTTAAGGCGGAGGAAAGGCCGGAAGCGCCCGCCCCCAAGGCCGAGGAGGCGCCCCGTCCCGCCCC
CAAGGAAGCCCCTCCCGCCCCCCAGGAGGCGCCCTCCGAGCGCCGCCTCATCCCGGCGGCCCCCTCCATAAGGCGGCTCG
CCCGGGAGCTCGGGGTGGACCTCACCCGGCTCCGGGGCACGGGGCTTGCCGGGCGCATCACCGAGGAGGACGTGCGCCGG
GCGGCGGGCCTTGGGGAGGCGGCCCCGGCGGCCCTTCCGGCGGCCCCCGCGCCCAGGCTTCCCGACTTCACCAAGTGGGG
CCCCGTGCGCCGGGAGCCCATGAGCGGGGTGCGCAAGGCCACGGTGCGCTCCATGAGCCAGGCCTGGGCCCAGGTGCCCA
TGGTCACCCACTTTGACGAGGCGGACGTCACCGAGCTGGAGGCTTTGCGCAAGCAGTACGCCAAGAAGGCCGAGGAGAAG
GGGTTCCGCCTCACCCTCACCGCCTTCCTCCTCAAGGCCCTGGCCCTCACCCTGAAGGCCTTCCCCAAGTTCAACGCCTC
CCTGGACGTGGAGGCCCAGGAGATCGTCTACAAGGACTACATCCACATCGGGGTGGCGGTGGACACGCCCCACGGCCTTT
TGGTCCCGGTGATCCGCGACGTGGACCGGAAAGGGGTCTTGCGCCTGGCGGAGGAGCTTCAGGAGATCTCCCAAAGGGCC
CGGGAGCGGAAGCTCTCCCCCGAGGAGATGCAGGGGGCCACCTTCAGCCTCTCCAACCTGGGGGGGATCGGCGGCACCGG
CTTCACCCCCATCGTCAACTGGCCGGAGGTGGCCATCCTCGGGGTCTCCCGCTCCCAGATGAAACCCCTTTGGGACCCCG
GGAAGGAGGCCTTCGTGCCCCGGCTCGTGATGCCCTTCAGCCTCACCTACGACCACCGCCTGATTGACGGGGCGGACGCC
GCCCGCTTCTGCCGCCACCTTGCGGGGATTCTGGAGGACCCCCTGGGCCTCGCCCTAGCCTAG

Upstream 100 bases:

>100_bases
CGGGGAGGGCAAGGTGACGAAGAGGACCCTGAACCAGGCGAGGAAGGACCTGGGCCTTAAGCTGGAGGAGGTGCCGCCCC
ACCGGCGGTAGGGGGAGAGC

Downstream 100 bases:

>100_bases
CCGGAGGAGGCGCTGGGGCGTGGCGATGAGGTCCACCCGCCTTTCCGGGGGCACGGGAAGGCGGGGATAGACCATCACCG
GGTGGGCGAGGGTGGCGAAG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MEVKLPELGDNVTQATVVGVLVKEGDRVEPGQPLLELETDKAVVEVPAEAGGVVKRVLVKVGDEVRPGQPFLELAEAEGG
AEAPPLKAEERPEAPAPKAEEAPRPAPKEAPPAPQEAPSERRLIPAAPSIRRLARELGVDLTRLRGTGLAGRITEEDVRR
AAGLGEAAPAALPAAPAPRLPDFTKWGPVRREPMSGVRKATVRSMSQAWAQVPMVTHFDEADVTELEALRKQYAKKAEEK
GFRLTLTAFLLKALALTLKAFPKFNASLDVEAQEIVYKDYIHIGVAVDTPHGLLVPVIRDVDRKGVLRLAEELQEISQRA
RERKLSPEEMQGATFSLSNLGGIGGTGFTPIVNWPEVAILGVSRSQMKPLWDPGKEAFVPRLVMPFSLTYDHRLIDGADA
ARFCRHLAGILEDPLGLALA

Sequences:

>Translated_420_residues
MEVKLPELGDNVTQATVVGVLVKEGDRVEPGQPLLELETDKAVVEVPAEAGGVVKRVLVKVGDEVRPGQPFLELAEAEGG
AEAPPLKAEERPEAPAPKAEEAPRPAPKEAPPAPQEAPSERRLIPAAPSIRRLARELGVDLTRLRGTGLAGRITEEDVRR
AAGLGEAAPAALPAAPAPRLPDFTKWGPVRREPMSGVRKATVRSMSQAWAQVPMVTHFDEADVTELEALRKQYAKKAEEK
GFRLTLTAFLLKALALTLKAFPKFNASLDVEAQEIVYKDYIHIGVAVDTPHGLLVPVIRDVDRKGVLRLAEELQEISQRA
RERKLSPEEMQGATFSLSNLGGIGGTGFTPIVNWPEVAILGVSRSQMKPLWDPGKEAFVPRLVMPFSLTYDHRLIDGADA
ARFCRHLAGILEDPLGLALA
>Mature_420_residues
MEVKLPELGDNVTQATVVGVLVKEGDRVEPGQPLLELETDKAVVEVPAEAGGVVKRVLVKVGDEVRPGQPFLELAEAEGG
AEAPPLKAEERPEAPAPKAEEAPRPAPKEAPPAPQEAPSERRLIPAAPSIRRLARELGVDLTRLRGTGLAGRITEEDVRR
AAGLGEAAPAALPAAPAPRLPDFTKWGPVRREPMSGVRKATVRSMSQAWAQVPMVTHFDEADVTELEALRKQYAKKAEEK
GFRLTLTAFLLKALALTLKAFPKFNASLDVEAQEIVYKDYIHIGVAVDTPHGLLVPVIRDVDRKGVLRLAEELQEISQRA
RERKLSPEEMQGATFSLSNLGGIGGTGFTPIVNWPEVAILGVSRSQMKPLWDPGKEAFVPRLVMPFSLTYDHRLIDGADA
ARFCRHLAGILEDPLGLALA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=438, Percent_Identity=32.4200913242009, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=29.185520361991, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=27.645788336933, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=37.9310344827586, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=27.645788336933, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI260898739, Length=163, Percent_Identity=36.1963190184049, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786305, Length=422, Percent_Identity=44.7867298578199, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI1786946, Length=420, Percent_Identity=33.5714285714286, Blast_Score=216, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=30.9133489461358, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17560088, Length=446, Percent_Identity=31.8385650224215, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=412, Percent_Identity=32.2815533980583, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=28.3387622149837, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=436, Percent_Identity=27.9816513761468, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=28.7912087912088, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=32.9466357308585, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI20129315, Length=230, Percent_Identity=30.8695652173913, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24582497, Length=230, Percent_Identity=30.8695652173913, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=35.3982300884956, Blast_Score=131, Evalue=9e-31,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45456; Mature: 45456

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKLPELGDNVTQATVVGVLVKEGDRVEPGQPLLELETDKAVVEVPAEAGGVVKRVLVK
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHH
VGDEVRPGQPFLELAEAEGGAEAPPLKAEERPEAPAPKAEEAPRPAPKEAPPAPQEAPSE
HCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RRLIPAAPSIRRLARELGVDLTRLRGTGLAGRITEEDVRRAAGLGEAAPAALPAAPAPRL
CCCCCCCHHHHHHHHHHCCCEEHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
PDFTKWGPVRREPMSGVRKATVRSMSQAWAQVPMVTHFDEADVTELEALRKQYAKKAEEK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHC
GFRLTLTAFLLKALALTLKAFPKFNASLDVEAQEIVYKDYIHIGVAVDTPHGLLVPVIRD
CCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCHHHHHHHC
VDRKGVLRLAEELQEISQRARERKLSPEEMQGATFSLSNLGGIGGTGFTPIVNWPEVAIL
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEHHCCCCCCCCCCCCCCCCCCEEEE
GVSRSQMKPLWDPGKEAFVPRLVMPFSLTYDHRLIDGADAARFCRHLAGILEDPLGLALA
ECCHHHCCCCCCCCHHHHCCHHHCCEEECCCCHHCCCCHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MEVKLPELGDNVTQATVVGVLVKEGDRVEPGQPLLELETDKAVVEVPAEAGGVVKRVLVK
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHH
VGDEVRPGQPFLELAEAEGGAEAPPLKAEERPEAPAPKAEEAPRPAPKEAPPAPQEAPSE
HCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RRLIPAAPSIRRLARELGVDLTRLRGTGLAGRITEEDVRRAAGLGEAAPAALPAAPAPRL
CCCCCCCHHHHHHHHHHCCCEEHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
PDFTKWGPVRREPMSGVRKATVRSMSQAWAQVPMVTHFDEADVTELEALRKQYAKKAEEK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHC
GFRLTLTAFLLKALALTLKAFPKFNASLDVEAQEIVYKDYIHIGVAVDTPHGLLVPVIRD
CCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCHHHHHHHC
VDRKGVLRLAEELQEISQRARERKLSPEEMQGATFSLSNLGGIGGTGFTPIVNWPEVAIL
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEHHCCCCCCCCCCCCCCCCCCEEEE
GVSRSQMKPLWDPGKEAFVPRLVMPFSLTYDHRLIDGADAARFCRHLAGILEDPLGLALA
ECCHHHCCCCCCCCHHHHCCHHHCCEEECCCCHHCCCCHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]