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The map label for this gene is rsuA [C]

Identifier: 55980117

GI number: 55980117

Start: 143466

End: 144197

Strand: Direct

Name: rsuA [C]

Synonym: TTHA0148

Alternate gene names: 55980117

Gene position: 143466-144197 (Clockwise)

Preceding gene: 55980116

Following gene: 55980123

Centisome position: 7.76

GC content: 70.08

Gene sequence:

>732_bases
ATGAAGGGGGAGAGGCTGGACAAAGTCCTCGCCCGGCTGGGCCTGGGAAGCCGCAAGGAGGTGGCCCGGCTGGTGCGCCA
AGGGAAGGTGCGGGTCCTGGGCCAGGTGGTGAAGGACCCCGGCTTCCGCGTGCCGGAGGGCGCCGCCTTGGAAGTGGAGG
GAAGACCCCTGGAGGTGCGCCGCCACCTCCACGTCCTCCTGCACAAGCCCCCAGGCTTCGTGACGAGCCGGACCGAGGGG
CCTTCTGTCTACGCCCTCCTTCAGGGCTTCCCCACCCGGGACCTCTCCCCGGTGGGCCGCCTGGACAAGGACGCCGAGGG
GCTTTTGCTCTTCACCACCGACGGCCTCCTCCTCCACCGCCTCACCCACCCCCGGCACAAGGTGGCCAAGCGCTACCTGG
TGCACTTGGAGAGGCCCGTGGGGCCTAAGGACCAGGAGGCCTTCCGGGAAGGAATACTCCTGGACGGCAAGAAGACCCTC
CCTGCCGAGCTCACCCCCCTGGAAGACCCCACCTGGGCCGAGGTGGTCCTCAAGGAGGGGCGGTTTCACCAGATCAAGCG
CATGTTCCGGGCCCGGGGCAACCGGGTTCTCCGCCTCAAGCGCCTGGCCCTGGGCCCCCTGGAGCTCGGCGACCTCCCCC
CGGGGAAGGCCCGCCCCCTCACGGAGGCGGAGGAAAAAGCCCTCTACGCCGCCGTGGGCCTAGGGGGCGAGGACCCTGAG
GACGAGGTCTAG

Upstream 100 bases:

>100_bases
TTGGGCACGCCCTTTTGCGCCTGGCCCCCTTACGGGGCCTGAACCGGGGCCGGGCGGAGGCCCAGGGGACCCTTTGGTGG
CTGGAGGGAGCCTCGGAGCG

Downstream 100 bases:

>100_bases
GGCGAGGCGCTCTCCCCGCTCGCCCACGGGCTCCAGGGTGTAGGCCTCCACCCAGAGGGGCTTCCCGAGCCCGGGGAGGC
CCTCGAGGTAGCCCAAGACC

Product: ribosomal large subunit pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKGERLDKVLARLGLGSRKEVARLVRQGKVRVLGQVVKDPGFRVPEGAALEVEGRPLEVRRHLHVLLHKPPGFVTSRTEG
PSVYALLQGFPTRDLSPVGRLDKDAEGLLLFTTDGLLLHRLTHPRHKVAKRYLVHLERPVGPKDQEAFREGILLDGKKTL
PAELTPLEDPTWAEVVLKEGRFHQIKRMFRARGNRVLRLKRLALGPLELGDLPPGKARPLTEAEEKALYAAVGLGGEDPE
DEV

Sequences:

>Translated_243_residues
MKGERLDKVLARLGLGSRKEVARLVRQGKVRVLGQVVKDPGFRVPEGAALEVEGRPLEVRRHLHVLLHKPPGFVTSRTEG
PSVYALLQGFPTRDLSPVGRLDKDAEGLLLFTTDGLLLHRLTHPRHKVAKRYLVHLERPVGPKDQEAFREGILLDGKKTL
PAELTPLEDPTWAEVVLKEGRFHQIKRMFRARGNRVLRLKRLALGPLELGDLPPGKARPLTEAEEKALYAAVGLGGEDPE
DEV
>Mature_243_residues
MKGERLDKVLARLGLGSRKEVARLVRQGKVRVLGQVVKDPGFRVPEGAALEVEGRPLEVRRHLHVLLHKPPGFVTSRTEG
PSVYALLQGFPTRDLSPVGRLDKDAEGLLLFTTDGLLLHRLTHPRHKVAKRYLVHLERPVGPKDQEAFREGILLDGKKTL
PAELTPLEDPTWAEVVLKEGRFHQIKRMFRARGNRVLRLKRLALGPLELGDLPPGKARPLTEAEEKALYAAVGLGGEDPE
DEV

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=227, Percent_Identity=40.0881057268723, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787524, Length=251, Percent_Identity=34.2629482071713, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790453, Length=231, Percent_Identity=28.1385281385281, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27073; Mature: 27073

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGERLDKVLARLGLGSRKEVARLVRQGKVRVLGQVVKDPGFRVPEGAALEVEGRPLEVR
CCCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHH
RHLHVLLHKPPGFVTSRTEGPSVYALLQGFPTRDLSPVGRLDKDAEGLLLFTTDGLLLHR
HHHHHEEECCCCCEECCCCCCCEEHEECCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHH
LTHPRHKVAKRYLVHLERPVGPKDQEAFREGILLDGKKTLPAELTPLEDPTWAEVVLKEG
HCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHCC
RFHQIKRMFRARGNRVLRLKRLALGPLELGDLPPGKARPLTEAEEKALYAAVGLGGEDPE
CHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHEEHEECCCCCCCC
DEV
CCC
>Mature Secondary Structure
MKGERLDKVLARLGLGSRKEVARLVRQGKVRVLGQVVKDPGFRVPEGAALEVEGRPLEVR
CCCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHH
RHLHVLLHKPPGFVTSRTEGPSVYALLQGFPTRDLSPVGRLDKDAEGLLLFTTDGLLLHR
HHHHHEEECCCCCEECCCCCCCEEHEECCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHH
LTHPRHKVAKRYLVHLERPVGPKDQEAFREGILLDGKKTLPAELTPLEDPTWAEVVLKEG
HCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHCC
RFHQIKRMFRARGNRVLRLKRLALGPLELGDLPPGKARPLTEAEEKALYAAVGLGGEDPE
CHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHEEHEECCCCCCCC
DEV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]