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The map label for this gene is lacA [C]

Identifier: 55980088

GI number: 55980088

Start: 119525

End: 120412

Strand: Reverse

Name: lacA [C]

Synonym: TTHA0119

Alternate gene names: 55980088

Gene position: 120412-119525 (Counterclockwise)

Preceding gene: 55980089

Following gene: 55980087

Centisome position: 6.51

GC content: 66.44

Gene sequence:

>888_bases
ATGCCCTGGCTTCTTCCCCGCGAGATCGCGCCCCTCCACCAAAAGGCCCTGGACCGCTATCTGGGTAGCCTCACGGAGAG
GCTTTCCGACCCCAATGTGGACCGGAACGCCTTGGTGCGGGAGGAGCTCGCCCGCCTCCTTTACGGGAGGCCCTACGAGG
AGCTTCTGGAGGCGAACCCGCTCGCGGCCATGGGCCTGGACCCGGAGGGGATCACCTTTGAGGCCGAGTACTACGCCGCC
ACCGACCTGGAGAAGTTCCGGCGGGTCAAGCCCCTCCTCTGGTTCTGGAAGGTCCTGGACCTCACGCCCTTGGGCCAGTC
GGTGCACTCGGGGGTGGCCATAAGGCGGGCCCTCGCCCCATTCATCTTCAAGCGGGTGGGGAAAAACCCCAAGTTCTTCC
AGAACGTGGAGTTCTCCGTGGGCTACAACCTGGAGCTTGGGGACGACGTGGTGGTGCACCGCTACGTCTTCCTGGACGAC
ATCGGCGGCATCAAGATCGGGGACCGCACCTCCCTCTCCGACTACGTCAACGTCTACAGCCACACCCACCACGTCCTGGC
CTCCCCCGACGTCACCCTGAAGGAGACCATCATCGGAAGCGGGGTCCGCATCACCTACCACGCCACCGTCTTGGCGGGGG
TGCGCATCGGGGACGACGCCATGGTGGGCACGGGGGCCGTCGTCACCAAGGACATCCCCCCCCACGCCATCGCCCTGGGC
ATCCCCGCGAGGCCCGTGCGCTACAAGGTCCGCCACGACTGCCCCTACTGCCGCAAGGGGGAGCCCCATCCCTCGGACCT
TGTTCCCAAGCTTCCCGACCGCAAGGGGAACCCCGACTACCCCGATTTCCTGCCCCCGGGCTTCGGAACAAGGGAGGCGG
GCTCTTGA

Upstream 100 bases:

>100_bases
GGCGCAAGGACCCCGAGGCCCTTCGGGAGCTCGGCTACCGCTCCTCCGTAGGGTGAGGCAGGAGGCTGTTTCCCCGGGCC
TTGGGGAGTAGCATGGGTTT

Downstream 100 bases:

>100_bases
CGGGGTGGGGCTTTTTTGGCTAAACTAGCGGGTGCGTGCGGGGGCGATTAGCTCAGCTGGTTAGAGCGCACGCCTGATAA
GCGTGAGGTCGGTGGTTCAA

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MPWLLPREIAPLHQKALDRYLGSLTERLSDPNVDRNALVREELARLLYGRPYEELLEANPLAAMGLDPEGITFEAEYYAA
TDLEKFRRVKPLLWFWKVLDLTPLGQSVHSGVAIRRALAPFIFKRVGKNPKFFQNVEFSVGYNLELGDDVVVHRYVFLDD
IGGIKIGDRTSLSDYVNVYSHTHHVLASPDVTLKETIIGSGVRITYHATVLAGVRIGDDAMVGTGAVVTKDIPPHAIALG
IPARPVRYKVRHDCPYCRKGEPHPSDLVPKLPDRKGNPDYPDFLPPGFGTREAGS

Sequences:

>Translated_295_residues
MPWLLPREIAPLHQKALDRYLGSLTERLSDPNVDRNALVREELARLLYGRPYEELLEANPLAAMGLDPEGITFEAEYYAA
TDLEKFRRVKPLLWFWKVLDLTPLGQSVHSGVAIRRALAPFIFKRVGKNPKFFQNVEFSVGYNLELGDDVVVHRYVFLDD
IGGIKIGDRTSLSDYVNVYSHTHHVLASPDVTLKETIIGSGVRITYHATVLAGVRIGDDAMVGTGAVVTKDIPPHAIALG
IPARPVRYKVRHDCPYCRKGEPHPSDLVPKLPDRKGNPDYPDFLPPGFGTREAGS
>Mature_294_residues
PWLLPREIAPLHQKALDRYLGSLTERLSDPNVDRNALVREELARLLYGRPYEELLEANPLAAMGLDPEGITFEAEYYAAT
DLEKFRRVKPLLWFWKVLDLTPLGQSVHSGVAIRRALAPFIFKRVGKNPKFFQNVEFSVGYNLELGDDVVVHRYVFLDDI
GGIKIGDRTSLSDYVNVYSHTHHVLASPDVTLKETIIGSGVRITYHATVLAGVRIGDDAMVGTGAVVTKDIPPHAIALGI
PARPVRYKVRHDCPYCRKGEPHPSDLVPKLPDRKGNPDYPDFLPPGFGTREAGS

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=142, Percent_Identity=33.8028169014084, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786664, Length=111, Percent_Identity=36.036036036036, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 32915; Mature: 32784

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWLLPREIAPLHQKALDRYLGSLTERLSDPNVDRNALVREELARLLYGRPYEELLEANP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCC
LAAMGLDPEGITFEAEYYAATDLEKFRRVKPLLWFWKVLDLTPLGQSVHSGVAIRRALAP
CEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FIFKRVGKNPKFFQNVEFSVGYNLELGDDVVVHRYVFLDDIGGIKIGDRTSLSDYVNVYS
HHHHHHCCCCCHHHCCEEEECCCEECCCCEEEEEEEEEECCCCEEECCCCCHHHHHHHHH
HTHHVLASPDVTLKETIIGSGVRITYHATVLAGVRIGDDAMVGTGAVVTKDIPPHAIALG
HCCEEEECCCCCHHHHHHCCCEEEEEEEEEEEEEEECCCCEECCCCEEECCCCCCEEEEC
IPARPVRYKVRHDCPYCRKGEPHPSDLVPKLPDRKGNPDYPDFLPPGFGTREAGS
CCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PWLLPREIAPLHQKALDRYLGSLTERLSDPNVDRNALVREELARLLYGRPYEELLEANP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCC
LAAMGLDPEGITFEAEYYAATDLEKFRRVKPLLWFWKVLDLTPLGQSVHSGVAIRRALAP
CEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FIFKRVGKNPKFFQNVEFSVGYNLELGDDVVVHRYVFLDDIGGIKIGDRTSLSDYVNVYS
HHHHHHCCCCCHHHCCEEEECCCEECCCCEEEEEEEEEECCCCEEECCCCCHHHHHHHHH
HTHHVLASPDVTLKETIIGSGVRITYHATVLAGVRIGDDAMVGTGAVVTKDIPPHAIALG
HCCEEEECCCCCHHHHHHCCCEEEEEEEEEEEEEEECCCCEECCCCEEECCCCCCEEEEC
IPARPVRYKVRHDCPYCRKGEPHPSDLVPKLPDRKGNPDYPDFLPPGFGTREAGS
CCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]